Bacteria Inspired Internal Standard SERS Substrate for Quantitative Detection

2020 ◽  
Author(s):  
Jiawei Liu ◽  
Zilan Hong ◽  
Weimin Yang ◽  
Chen Liu ◽  
Zhicheng Lu ◽  
...  
Keyword(s):  
Internal Standard ◽  
Quantitative Detection ◽  
Sers Substrate

scholarly journals Fabrication and Characterization of a Highly-Sensitive Surface-Enhanced Raman Scattering Nanosensor for Detecting Glucose in Urine

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 629 ◽  
Author(s):  
Yudong Lu ◽  
Ting Zhou ◽  
Ruiyun You ◽  
Yang Wu ◽  
Huiying Shen ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Internal Standard ◽  
Surface Enhanced Raman Scattering ◽  
Quantitative Detection ◽  
Core Shell ◽  
Ag Nps ◽  
Vis Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Herein we utilized coordination interactions to prepare a novel core-shell plasmonic nanosensor for the detection of glucose. Specifically, Au nanoparticles (NPs) were strongly linked with Ag+ ions to form a sacrificial Ag shell by using 4-aminothiophenol (4-PATP) as a mediator, which served as an internal standard to decrease the influence of the surrounding on the detection. The resultant Au-PATP-Ag core-shell systems were characterized by UV-vis spectroscopy, transmission electron microscopy, and surface-enhanced Raman scattering (SERS) techniques. Experiments performed with R6G (rhodamine 6G) and CV (crystal violet) as Raman reporters demonstrated that the Au@Ag nanostructure amplified SERS signals obviously. Subsequently, the Au@Ag NPs were decorated with 4-mercaptophenylboronic acid (4-MPBA) to specifically recognize glucose by esterification, and a detection limit as low as 10−4 M was achieved. Notably, an enhanced linearity for the quantitative detection of glucose (R2 = 0.995) was obtained after the normalization of the spectral peaks using 4-PATP as the internal standard. Finally, the practical applicability of the developed sensing platform was demonstrated by the detection of glucose in urine with acceptable specificity.

scholarly journals Application of Aluminum Hydroxide for Improvement of Label-Free SERS Detection of Some Cephalosporin Antibiotics in Urine

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 91 ◽  
Author(s):  
Natalia E. Markina ◽  
Alexey V. Markin
Keyword(s):  
Aluminum Hydroxide ◽  
Surface Enhanced Raman Spectroscopy ◽  
Negative Influence ◽  
Quantitative Detection ◽  
Therapeutic Drug ◽  
Excitation Wavelength ◽  
Sers Substrate ◽  
Label Free ◽  
Surface Enhanced Raman ◽  
Sers Detection

This report is dedicated to development of surface-enhanced Raman spectroscopy (SERS) based analysis protocol for detection of antibiotics in urine. The key step of the protocol is the pretreatment of urine before the detection to minimize background signal. The pretreatment includes extraction of intrinsic urine components using aluminum hydroxide gel (AHG) and further pH adjusting of the purified sample. The protocol was tested by detection of a single antibiotic in artificially spiked samples of real urine. Five antibiotics of cephalosporin class (cefazolin, cefoperazone, cefotaxime, ceftriaxone, and cefuroxime) were used for testing. SERS measurements were performed using a portable Raman spectrometer with 638 nm excitation wavelength and silver nanoparticles as SERS substrate. The calibration curves of four antibiotics (cefuroxime is the exception) cover the concentrations required for detection in patient’s urine during therapy (25/100‒500 μg/mL). Random error of the analysis (RSD < 20%) and limits of quantification (20‒90 μg/mL) for these antibiotics demonstrate the applicability of the protocol for reliable quantitative detection during therapeutic drug monitoring. The detection of cefuroxime using the protocol is not sensitive enough, allowing only for qualitative detection. Additionally, time stability and batch-to-batch reproducibility of AHG were studied and negative influence of the pretreatment protocol and its limitations were estimated and discussed.

scholarly journals Insight into the working wavelength of hotspot effects generated by popular nanostructures

2019 ◽  
Vol 8 (1) ◽  
pp. 24-34 ◽  
Author(s):  
Zhong Wang ◽  
Kesu Cai ◽  
Yang Lu ◽  
Haining Wu ◽  
Yuee Li ◽  
...  
Keyword(s):  
Electric Field ◽  
Field Enhancement ◽  
Quantitative Detection ◽  
Excitation Wavelength ◽  
Sers Substrate ◽  
Nanorod Arrays ◽  
Electric Field Enhancement ◽  
Incident Laser ◽  
Incident Wavelength ◽  
Excitation Laser

Abstract A proper excitation wavelength is much important for the application of surface-enhanced Raman spectroscopy (SERS) in the biochemical field. Here, based on a SERS substrate model with an incident Gaussian beam, we investigate the dependence of the electric field enhancement on the incident wavelength of the excitation laser for popular nanostructures, including nanosphere dimer, nanorod dimer, and nanorod arrays. The results in the present manuscript indicate that both the nanosphere and nanorod dimer present a much broader plasmonic excitation wavelength range extending to the near-infrared region. The enhancement effect of Nanorod arrays is strongly dependent on the incident direction of excitation light. Finally, according to the conclusions above, a SERS substrate consisting of nanocubes based on the SPP eigen-mode is proposed and the electric field enhancement is homogeneous, and insensitive to the polarization of the incident laser. The enhancement factor is not ultrahigh; however, good homogeneousness permits for quantitative detection of lower concentration components in mixtures. Graphical abstract: By investigating the dependence of the electric field enhancement on the incident wavelength of the excitation laser for popular nanostructures, we propose a SERS substrate consisting of Au nanocubes based on the SPP eigenmode. The electric field enhancement is homogeneous, and insensitive to the polarization of the incident laser. Though the enhancement factor is not ultrahigh, good homogeneousness permits for quantitative detection of lower concentration components in mixtures.

scholarly journals Quantitative Reverse Transcription-PCR Assay with an Internal Standard for the Detection of Prostate-specific Antigen mRNA

1999 ◽  
Vol 45 (9) ◽  
pp. 1397-1407 ◽  
Author(s):  
Alice Ylikoski ◽  
Minna Sjöroos ◽  
Åke Lundwall ◽  
Matti Karp ◽  
Timo Lövgren ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Detection Limit ◽  
Prostate Specific Antigen ◽  
Reverse Transcription ◽  
Specific Antigen ◽  
Internal Standard ◽  
Quantitative Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

Abstract Background: Circulating prostate cells can be detected with a reverse transcription-PCR (RT-PCR) assay for prostate-specific antigen (PSA) mRNA. We have developed a new quantitative RT-PCR method for measuring PSA mRNA. Methods: The method uses a PSA-like internal standard (IS) mRNA that is added into the sample at the beginning of the RNA extraction and coamplified by RT-PCR with the PSA in the sample. After PCR amplification, the IS and PSA products are selectively detected by hybridization in a microtitration plate using probes labeled with fluorescent europium chelates. Results: The method was validated with PSA and IS mRNAs and PSA-expressing cells to obtain a detection limit of 50 PSA mRNA copies (i.e., signal 2 times the mean of zero signal), linearity up to 106 copies, and detection of a single PSA-expressing cell. In preliminary evaluations, 60% (n = 10) of the prostate cancer patients with skeletal metastases gave results above the detection limit (500 PSA mRNA copies in 5 mL of blood). The total number of PSA copies ranged from 900 ± 200 to 44 100 ± 4900 (mean ± SD) in the samples, corresponding to ∼1–100 PSA-expressing cells in 5 mL of blood. In the controls (n = 34), none of the healthy females and 2 of 19 healthy males had detectable PSA mRNA [700 ± 100 and 2000 ± 900 (mean ± SD) PSA mRNA copies in 5 mL of blood for the 2 males]. Conclusions: The assay provides sensitive and quantitative detection of PSA mRNA expression from blood samples and can be used to establish the clinically significant number of PSA mRNA copies in prostate cancer.

Modified method of erythropoietin (ESF) bioassay in vitro using mouse fetal liver cells. II. measurement of ESF in human serum

Blood ◽  
1978 ◽  
Vol 52 (3) ◽  
pp. 569-577 ◽  
Author(s):  
G de Klerk ◽  
AA Hart ◽  
C Kruiswijk ◽  
R Goudsmit
Keyword(s):  
Human Serum ◽  
Fetal Liver ◽  
Internal Standard ◽  
Immune Serum ◽  
Quantitative Detection ◽  
Modified Method ◽  
The Difference ◽  
Normal Males ◽  
Test Serum

Abstract A modification of the mouse fetal liver cell bioassay for erythropoietin (ESF) that allows quantitative detection of ESF in human serum is described. The modification consists of (1) correction for the effect of serum iron on 59Fe incorporation into heme and (2) the use of an “internal standard,” i.e., a standard ESF preparation dissolved in the assayed test serum. As a result of this modification the statistical method of bioassay analysis had to be changed fundamentally. The mean serum concentration of ESF measured in 20 normal males was 48 microunit/ml, as compared to 29 microunit/ml in 18 females. The difference was significant at p = 0.017. The stimulatory activity of a human serum on 59Fe incorporation into heme could be neutralized by an anti-human ESF immune serum.

scholarly journals Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics

2021 ◽  
Author(s):  
Jing Zhou ◽  
Hongzhe Wang ◽  
Caiyun Miao ◽  
Yunxi Yao ◽  
Jianshe Ma
Keyword(s):  
Matrix Effects ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Absolute Bioavailability ◽  
Quantitative Detection ◽  
Ionization Source ◽  
Mouse Blood ◽  
The Matrix ◽  
Product Ions

AbstractA rapid and simple UPLC-MS/MS method was developed to determine toddalolactone in mouse blood and applied to measure the pharmacokinetics of toddalolactone in mice. Blood samples were first preprocessed by ethyl acetate liquid-liquid extraction. Oxypeucedanin hydrate (internal standard, IS) and toddalolactone were gradient eluted from a UPLC BEH C18 column using a mobile phase consisting of acetonitrile and water (0.1% formic acid). Using electrospray ionization (ESI) as the ionization source, multiple reaction monitoring was used to detect the precursor and product ions of m/z 309.2 and 205.2, respectively, for toddalolactone and of m/z 305.1 and 203.0 for IS, respectively, for quantitative detection. A calibration curve was run over the concentration range of 5–4,000 ng/mL (r > 0.995). The matrix effects ranged from 93.5 to 98.4%, and the recovery was higher than 77.3%. The precision was less than 13%, and the accuracy ranged from 90.9 to 108.4%. The developed UPLC-MS/MS method was successfully used for measuring the pharmacokinetics of toddalolactone in mice after oral (20 mg/kg) and intravenous administration (5 mg/kg), and the absolute bioavailability of toddalolactone was 22.4%.

scholarly journals Pharmacokinetics and bioavailability of liensinine in mouse blood by UPLC-MS/MS

2020 ◽  
Author(s):  
Shuhua Tong ◽  
Yuqi Zeng ◽  
Jianshe Ma ◽  
Congcong Wen
Keyword(s):  
Pharmacokinetic Study ◽  
Mobile Phase ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Quantitative Detection ◽  
Reaction Monitoring ◽  
Mouse Blood ◽  
Blood Samples ◽  
The Matrix ◽  
Better Than

AbstractLiensinine is a bisbenzyltetrahydroisoquinoline alkaloid extracted from lotus (Nelumbo nucifera GAERTNER., Nelumbonaceae), especially in its embryo loti “Lien Tze Hsin” (green embryo of mature seed). A rapid and simple UPLC-MS/MS method was developed to determine liensinine in mouse blood and its application to a pharmacokinetic study. The blood samples were preprocessed by protein precipitation using acetonitrile. Midazolam (internal standard, IS) and liensinine were gradient eluted by mobile phase of methanol and water (0.1% formic acid) in a Waters UPLC BEH C18 column. The multiple reaction monitoring of m/z 611.3 → 206.1 for liensinine and m/z 326.2 → 291.1 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 400 ng/mL (r > 0.995). The accuracy ranged from 92.2 to 108.2%, the precision of intra-day and inter-day was less than 14%, and the matrix effect was between 100.0% and 109.6%, the recovery was better than 71.0%. The developed UPLC-MS/MS method was successfully used for a pharmacokinetic study of liensinine in mice after oral (5 mg/kg) and intravenous administration (1 mg/kg), and the absolute availability of liensinine was 1.8%.

Quantitative detection of crystal violet using a surface-enhanced Raman scattering based on flower-like HAp/Ag nanocomposite

2021 ◽  
Author(s):  
Yamin Lin ◽  
Mengmeng Zheng ◽  
Xin Zhao ◽  
Dan Liu ◽  
Jiamin Gao ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Raman Scattering ◽  
Crystal Violet ◽  
Quantitative Detection ◽  
Sers Substrate ◽  
One Pot ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Herein, we proposed a simple one-pot sol-thermal strategy to prepare highly sensitive and reproducible SERS substrate. The silver-doped hydroxyapatite nanocomposite (HAp/Ag) could suppress the oxidation of the silver nanoparticles, which...

scholarly journals Determination of eugenitin in mouse blood by ultra-performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study

2021 ◽  
Author(s):  
Chongliang Lin ◽  
Dezhen Song ◽  
Haodong Jiang ◽  
Lvqi Luo ◽  
Xi Bao ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Multiple Reaction Monitoring ◽  
Internal Standard ◽  
Ultra Performance Liquid Chromatography ◽  
Quantitative Detection ◽  
Syzygium Aromaticum ◽  
Mouse Blood ◽  
The Matrix ◽  
Liquid Chromatography Tandem Mass

Abstract Eugenitin is a non-volatile chromone derivative which is always found in dried flower buds of Syzygium aromaticum L. (Merr.) & L.M. Perry. Until now, there were no reports about the pharmacokinetics of eugenitin in biological fluids. A UPLC-MS/MS method developed to determine eugenitin in mouse blood. The blood samples were prepared by protein precipitation with acetonitrile. Chrysin (internal standard, IS) and eugenitin were gradient eluted by mobile phase of acetonitrile and water (0.1% formic acid) in a BEH C18 column. The multiple reaction monitoring (MRM) of m/z 221.1→206.0 for eugenitin and m/z 255.1→152.9 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 500 ng/mL (r > 0.995). The accuracy ranged from 98 to 113%, the precision was less than 12%, and the matrix effect was between 86 and 94%, the recovery was better than 81%. The developed method was successfully used for pharmacokinetics of eugenitin in mice after intravenous (5 mg/kg) and oral (20 mg/kg) administration, and the absolute availability of eugenitin was 12%.

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