Effect of Post-Treatment on Mechanical and Biological Properties of Coaxial Electrospun Core–Shell Structured Poly(lactic-co-glycolic acid)/Gelatin Methacrylamide Fibrous Scaffolds

Silver nanoparticles synthesized with polymers as coating agents is an effective method to overcome their poor stability and aggregation in solution. Silver-polyethylene glycol (Ag-PEG) nanoparticles were synthesized with the thiol-functionalized polyethylene glycol (SH-PEA) as the coating, reducing and stabilizing agent. The UV irradiation time, polymer and silver nitrate concentration for the synthesis were investigated. The concentration of silver nitrate had significant effect on the morphology of Ag-PEG nanoparticles. When increasing the concentration of silver nitrate, SEM and TEM images showed that Ag-PEG nanoparticles changed from Janus to multi-core shell structure. Meanwhile, pure silver particles in the two hybrid nanoparticles presented spherical shape and had the similar size of 15 nm. The antibacterial activities and cytotoxicity of the two structural Ag-PEG nanoparticles were investigated to understand colloid morphology effect on the properties of AgNPs. The results of antibacterial activities showed that the two structural Ag-PEG nanoparticles exhibited strong antibacterial activities against Staphylococcus aureus, Escherichia coli and Bacillus subtilis. The Janus nanoparticles had larger minimal inhibitory concentration (MIC) and minimum bacterial concentration (MBC) values than the multi-core shell counterparts. The results of cytotoxicity showed the Janus Ag-PEG nanoparticles had lower toxicity than the multi-core shell nanoparticles.

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Highly Stable CsPbX 3 /PbSO 4 Core/Shell Nanocrystals Synthesized by a Simple Post‐Treatment Strategy

Advanced Optical Materials ◽

10.1002/adom.202001763 ◽

2020 ◽

pp. 2001763

Author(s):

Qixuan Zhong ◽

Jun Liu ◽

Shuhua Chen ◽

Pengli Li ◽

Jianian Chen ◽

...

Keyword(s):

Treatment Strategy ◽

Post Treatment ◽

Core Shell

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Influence of porosity and pore shape on structural, mechanical and biological properties of polyϵ-caprolactone electro-spun fibrous scaffolds

Nanomedicine ◽

10.2217/nnm.16.21 ◽

2016 ◽

Vol 11 (9) ◽

pp. 1031-1040 ◽

Cited By ~ 22

Author(s):

Kieran P Fuller ◽

Diana Gaspar ◽

Luis M Delgado ◽

Abhay Pandit ◽

Dimitrios I Zeugolis

Keyword(s):

Biological Properties ◽

Pore Shape ◽

Fibrous Scaffolds

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Effects of Dynamic Fluid Pressure on Chondrocytes Cultured in Biodegradable Poly(glycolic acid) Fibrous Scaffolds

Tissue Engineering ◽

10.1089/ten.2005.11.1852 ◽

2005 ◽

Vol 11 (11-12) ◽

pp. 1852-1859 ◽

Cited By ~ 3

Author(s):

Lichun Lu ◽

Xun Zhu ◽

Larry G. Pederson ◽

Esmaiel Jabbari ◽

Bradford Currier ◽

...

Keyword(s):

Glycolic Acid ◽

Fluid Pressure ◽

Fibrous Scaffolds ◽

Biodegradable Poly

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Study on Preparation and Properties of Coaxial Electrospun PLGA/Gelatin Fibers

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1032.35 ◽

2021 ◽

Vol 1032 ◽

pp. 35-41

Author(s):

Jia Li ◽

Shuai Lin ◽

Yue Long Ma ◽

Xian Hua Xu ◽

Wei Zheng ◽

...

Keyword(s):

Glycolic Acid ◽

Coaxial Electrospinning ◽

Electrospun Fibers ◽

Core Shell ◽

High Porosity ◽

Large Specific Surface Area ◽

The Core ◽

Acid Copolymer ◽

Biomedical Field ◽

Core Shell Structure

The coaxial electrospun fibers with large specific surface area, high porosity and core-shell structure have been great applied in biomedical field, especially as drug delivery carriers. In this paper, PLGA(polylactic acid/glycolic acid copolymer) was used as the core and the mixture of PLGA and gelatin was used as the shell. PLGA/gelatin fiber was prepared by coaxial electrospinning technology. The effects of different parameters on the surface morphology and the diameter of fibers were investigated.

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Fabrication of Core-Shell PLGA-Chitosan Microparticles Using Electrospinning: Effects of Polymer Concentration

International Journal of Polymer Science ◽

10.1155/2017/9580209 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Nguyen Thi Hiep ◽

Nguyen Dai Hai ◽

Vo Van Toi

Keyword(s):

Glycolic Acid ◽

Polymer Concentration ◽

Core Shell ◽

The Core ◽

Ft Ir ◽

Ejection Speed ◽

Diphenyl Tetrazolium Bromide ◽

Product Outcome ◽

Labeling Method ◽

Fluorescence Observation

This investigation aims to fabricate the core-shell microparticles composed of poly(lactic-co-glycolic acid) and chitosan (PLGA-CS MPs) using electrospinning. The challenge of using electrospinning is that it has many parameters which change product outcome if any single parameter is changed. However, the advantage of this system is that we can fabricate either micro/nanofibers or micro/nanoparticles. To learn about the effect of liquid concentration, the electrospinning parameters (voltage, needle sizes, distance from needle to collector, and ejection speed) were fixed while the concentration of PLGA or chitosan was varied. The results showed that PLGA microparticles can be fabricated successfully when the concentration of PLGA is smaller than 10 wt%. Presence of the chitosan shell was confirmed by zeta potential measurements, FT-IR, optical observation, and fluorescence observation. Thickness of the chitosan shell can be controlled by changing the concentration of chitosan and measured by fluorescamine labeling method. Moreover, SEM observation showed that concentration of chitosan affected the size of PLGA-CS microparticles. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay test showed that PLGA-CS microparticles possess excellent biocompatibility.

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Brinzolamide loaded core-shell nanoparticles for enhanced coronial penetration in the treatment of glaucoma

Journal of Applied Biomaterials & Functional Materials ◽

10.1177/2280800020942712 ◽

2020 ◽

Vol 18 ◽

pp. 228080002094271

Author(s):

Jing Song ◽

Ziping Zhang

Keyword(s):

Glycolic Acid ◽

Neurodegenerative Disorder ◽

Treatment Strategies ◽

Entrapment Efficiency ◽

Single Step ◽

Core Shell ◽

Shell Nanoparticles ◽

Cytotoxicity Studies ◽

Electrospray Process ◽

Core Shell Nanoparticles

A neurodegenerative disorder, glaucoma is a leading cause of blindness in the world. The conventional treatment strategies do not allow the significant penetration of the drug in the cornea. Therefore, we prepare a brinzolamide (Brz) loaded core-shell nanoparticles (NPs) to enhance the coronial penetration of the drug and thus treating the glaucoma. The shell of the NPs was composed of phosphatidylserine (PS; 1,2-diacyl-sn-glycero-3-phospho-L-serine), whereas the core of the NPs contains the Brz encapsulated in brinzolamide–phosphatidylserine–polymer poly-(DL-lactic acid-co-glycolic acid)–phosphatidylserine (Brz-PS-PLGA). The synthesis of Brz-PS-PLGA was achieved by using a coaxial electrospray process (CEP), which allows the preparation of the particles in a single step. The size of Brz-PS-PLGA with PS shell and brinzolamide–poly (lactic-co-glycolic) acid (Brz-PLGA) without shell was 571 ± 27.02 nm and 456 ± 19.17 nm, respectively. The charges on the surface of Brz-PS-PLGA and Brz-PLGA were (-) 27.45 ± 2.98 mV and (-) 19.47 ± 2.83 mV. The transmission electron microscopy images clearly reveal the PS shell as a light black layer over the dark black PLGA core. The CEP allows the high encapsulation of Brz in Brz-PS-PLGA where percentage of entrapment efficiency for Brz-PS-PLGA was 88.13 ± 6.43%. The release study conducted in a simulated tear fluid revealed the sustained release patterns of Brz from Brz-PS-PLGA and these were nontoxic to the cells as revealed by the cytotoxicity studies. Further, the Brz-PS-PLGA enhanced the coronial penetration of Brz and was capable of significantly reducing the intraocular pressure (IOP) after administration to the rabbit eye in comparison to the Brz-PLGA and free Brz. The results clearly suggest that the PS coating significantly enhances the capability of the particles in reducing IOP.

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