Fabrication of Hybrid Hydrogels from Silk Fibroin and Tannic Acid with Enhanced Gelation and Antibacterial Activities

A medical adhesive called TASK based on polyphenol-gel systems was developed and showed wet-adhesive, stretchable, self-healing and restorable properties.

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Study on improving antioxydant and antibacterial activities of silk fibroin by irradiation treatment

Nuclear Science and Technology ◽

10.53747/jnst.v4i2.224 ◽

2014 ◽

Vol 4 (2) ◽

pp. 21-26

Author(s):

Bang Diep Tran ◽

Van Binh Nguyen ◽

Phuong Thao Hoang ◽

Dang Sang Hoang ◽

Huong Trang Nguyen Thuy

Keyword(s):

Silk Fibroin ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Solvent System ◽

Antibacterial Activities ◽

E Coli ◽

High Antibacterial Activity ◽

Irradiation Treatment ◽

Dose Ranging ◽

Dpph Radical Scavenging

The silk fibroin solutions were prepared in solvent system of CaCl2. CH3CH2OH. H2O (mole ratio = 1:2:8) followed dialysis against deionized water. The 3% silk fibroin solutions were irradiated under gamma Co-60 source with dose ranging from 0 to 50 kGy at Hanoi Irradiation Centre and bio-activities of the irradiated silk fibroin solutions were investigated with different radiation doses. The results indicated that the antioxidant and antibacterial activities of fibroin were much improved by gamma irradiation. Maximum value of DPPH radical scavenging activity was 70.4% for the solution of silk fibroin irradiated at 10 kGy. Silk fibroin solutions irradiated at doses higher than 10 kGy also exhibited rather high antibacterial activity against E. coli and S. aureus. In order to estimate the applicability of our irradiated fibroin, the silk fibroin solutions were lyophilized to obtain a pure fibroin powder, then their bio-activities were compared with those of commercial silk fibroin (Proteines De Soie/ Zijdeproteine, Bioflore, Canada). Our fibroin powder revealed higher antioxidant and antibacterial activities. The amino acid compositions of our irradiated fibroin were also higher than that of the commercial product. Thus, the irradiated silk fibroin can be used for further application in cosmetic and other related fields.

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Robust and Tunable Hybrid Hydrogels from Photo-Cross-Linked Soy Protein Isolate and Regenerated Silk Fibroin

ACS Sustainable Chemistry & Engineering ◽

10.1021/acssuschemeng.9b00147 ◽

2019 ◽

Vol 7 (10) ◽

pp. 9257-9271 ◽

Cited By ~ 8

Author(s):

Pramod Dorishetty ◽

Rajkamal Balu ◽

Anjitha Sreekumar ◽

Liliana de Campo ◽

Jitendra P. Mata ◽

...

Keyword(s):

Silk Fibroin ◽

Soy Protein ◽

Soy Protein Isolate ◽

Protein Isolate ◽

Hybrid Hydrogels ◽

Regenerated Silk Fibroin

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Biomimetic Mineralization of Tannic Acid-Supplemented HEMA/SBMA Nanocomposite Hydrogels

Polymers ◽

10.3390/polym13111697 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1697

Author(s):

Tai-Yu Chen ◽

Shih-Fu Ou ◽

Hsiu-Wen Chien

Keyword(s):

Mechanical Properties ◽

Water Content ◽

Tannic Acid ◽

Chemical Structure ◽

Fold Increase ◽

Biomimetic Mineralization ◽

X Ray ◽

Nanocomposite Hydrogels ◽

Hybrid Hydrogels ◽

Structure Water

This study developed a tannic acid (TA)-supplemented 2-hydroxyethyl methacrylate-co-sulfobetaine methacrylate (HEMA-co-SBMA) nanocomposite hydrogel with mineralization and antibacterial functions. Initially, hybrid hydrogels were synthesized by incorporating SBMA into the HEMA network and the influence of SBMA on the chemical structure, water content, mechanical properties, and antibacterial characteristics of the hybrid HEMA/SBMA hydrogels was examined. Then, nanoclay (Laponite XLG) was introduced into the hybrid HEMA/SBMA hydrogels and the effects evaluated of the nanoclay on the chemical structure, water content, and mechanical properties of these supplemented hydrogels. The 50/50 hybrid HEMA/SBMA hydrogel with 30 mg/mL nanoclay showed outstanding mechanical properties (3 MPa) and water content (60%) compared to pure polyHEMA hydrogels. TA then went on to be incorporated into these hybrid nanocomposite hydrogels and its effects investigated on biomimetic mineralization. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) showed that bone-like spheroidal precipitates with a Ca/P ratio of 1.67% were observed after 28 days within these mineralized hydrogels. These mineralized hydrogels demonstrated an almost 1.5-fold increase in compressive moduli compared to the hydrogels without mineralization. These multifunctional hydrogels display good mechanical and biomimetic properties and may have applications in bone regeneration therapies.

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Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function

Materials ◽

10.3390/ma13020285 ◽

2020 ◽

Vol 13 (2) ◽

pp. 285 ◽

Cited By ~ 1

Author(s):

Ahui Liang ◽

Min Zhang ◽

Hong Luo ◽

Longxing Niu ◽

Yanfei Feng ◽

...

Keyword(s):

Silk Fibroin ◽

Wound Dressing ◽

Freeze Drying ◽

Slow Release ◽

Antibacterial Activities ◽

Mass Ratio ◽

And Staphylococcus Aureus ◽

Positively Charged ◽

Porous Poly ◽

Effective Growth

In order to endue silk fibroin (SF) sponges with antibacterial function, positively charged poly(hexamethylene biguanide) hydrochloride (PHMB) was incorporated in SF through electrostatic interaction and by freeze-drying technique. The influence of PHMB on the structure and antibacterial activities of SF sponges was investigated. The zeta potential of SF was increased significantly when PHMB was incorporated in SF. The pores with size from 80 to 300 µm and the microscale holes in the pore walls within PHMB-loaded SF sponges provided the channels of PHMB release. The PHMB loaded in the porous sponges showed continuous and slow release for up to 20 days. Effective growth inhibition of both Escherichia coli and Staphylococcus aureus was achieved when the mass ratio of PHMB/SF was higher than 2/100. These results suggest that the porous PHMB/SF sponges have the potential to be used as a novel wound dressing for open skin wounds.

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3D Bioprinting of Cardiovascular Tissues for In Vivo and In Vitro Applications Using Hybrid Hydrogels Containing Silk Fibroin: State of the Art and Challenges

Current Tissue Microenvironment Reports ◽

10.1007/s43152-020-00026-5 ◽

2020 ◽

Vol 1 (4) ◽

pp. 261-276

Author(s):

Laura Vettori ◽

Poonam Sharma ◽

Jelena Rnjak-Kovacina ◽

Carmine Gentile

Keyword(s):

Silk Fibroin ◽

Mechanical Stability ◽

Biological Properties ◽

Cardiac Cells ◽

3D Bioprinting ◽

Natural Polymers ◽

Cardiovascular Tissues ◽

Hybrid Hydrogels

Abstract Purpose of Review 3D bioprinting of cardiovascular tissues for in vitro and in vivo applications is currently investigated as a potential solution to better mimic the microenvironment typical of the human heart. However, optimal cell viability and tissue vascularization remain two of the main challenges in this regard. Silk fibroin (SF) as a natural biomaterial with unique features supports cell survival and tissue vascularization. This review aims to evaluate the potential of hydrogels containing SF in 3D bioprinting of cardiac tissue that better recapitulate the native cardiac microenvironment. Recent Findings SF hydrogels spontaneously develop nanocrystals, which limit their use for 3D bioprinting applications. Nevertheless, the printability of SF is improved in hybrid hydrogels by mixing it with other natural polymers (such as alginate and gelatin). This is achieved by adding SF with other polymers or by crosslinking it by peroxidase catalysis (i.e., with alginate). Compared to only SF-based hydrogels, hybrid hydrogels provide a durable bioprinted construct with improved mechanical stability and biological properties. To date, studies using cardiac cells in bioprinted SF constructs are yet to be performed. Summary Mixing SF with other polymers in bioprinted hybrid hydrogels improves the printability and durability of 3D bioprinted tissues. Studies using these hydrogels with cardiac cells will be required to evaluate the biocompatibility of SF hybrid hydrogels and to establish their potential use for cardiovascular applications.

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