A Mass Spectrometry Screening Method for Antiaggregatory Activity of Proteins Covalently Modified by Combinatorial Library Members:  Application to Sickle Hemoglobin

2000 ◽  
Vol 2 (4) ◽  
pp. 314-317 ◽  
Author(s):  
Soobong Park ◽  
Linda Wanna ◽  
Michael E. Johnson ◽  
Duane L. Venton
Keyword(s):  
Mass Spectrometry ◽  
Combinatorial Library ◽  
Screening Method ◽  
Sickle Hemoglobin ◽  
Antiaggregatory Activity

Drug screening method development for paper spray coupled to a triple quadrupole mass spectrometer

2017 ◽  
Vol 9 (34) ◽  
pp. 5037-5043 ◽  
Author(s):  
Rachel Jett ◽  
Christine Skaggs ◽  
Nicholas E. Manicke
Keyword(s):  
Mass Spectrometry ◽  
Drug Screening ◽  
Method Development ◽  
Screening Method ◽  
Direct Analysis ◽  
Triple Quadrupole ◽  
Analysis Method ◽  
Quadrupole Mass ◽  
Triple Quadrupole Mass Spectrometer ◽  
Paper Spray

Paper spray mass spectrometry is a direct analysis method in which compounds are extracted and ionized from biofluids dried on paper.

Gas-Liquid Chromatographic/Mass Spectrometric Screening Method for T-2 Toxin in Milk

1979 ◽  
Vol 62 (6) ◽  
pp. 1274-1280
Author(s):  
George J Collins ◽  
Joseph D Rosen
Keyword(s):  
Mass Spectrometry ◽  
Electron Impact ◽  
Chemical Ionization ◽  
Impact Ionization ◽  
Trimethylsilyl Ether ◽  
Screening Method ◽  
Internal Standard ◽  
Electron Impact Ionization ◽  
Gas Liquid Chromatography ◽  
Ionization Mass

Abstract A method for the analysis of T-2 toxin in milk is presented. Ethyl acetate extracts of milk samples which had been spiked with T-2 toxin were purified by thin layer chromatography and derivatized with N,O-bis(trimethylsilyl)acetamide to produce the T-2 toxin trimethylsilyl ether (T-2 toxin-TMS). N,O-bis(trimethylsilyl-d9)acetamide was used to make T-2 toxin d9-trimethylsilyl ether (T-2 toxin-d9TMS) which was added to the derivatized milk extract as an internal standard. Samples were analyzed by combined gas-liquid chromatography/mass spectrometry using either electron impact ionization or chemical ionization mass spectrometry. In electron impact ionization analyses, simultaneous monitoring of the T-2 toxin-TMS fragment ion at m/z 436 and the T-2 toxin-d9TMS fragment ion at m/z 445 gave a T-2 toxin-TMS detectability estimated at 6 μg/kg. In chemical ionization analyses, the T-2 toxin-TMS fragment ion at m/z 377 and the T-2 toxin-d9TMS fragment ion at m/z 386 were simultaneously monitored to give a T-2 toxin-TMS detectability estimated at 3 μg/kg. Average recovery was 85% at 200 μg/kg and 65% at 20 μg/kg.

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