Syntheses, characterizations, and interconversion reactions of cis- and trans-bis(2,4-pentanedionato)diaquachromium(III) complexes. Application to the cleavage reactions of the chromium-carbon bonds in trans-(dichloromethyl and chloromethyl)bis(2,4-pentanedionato)aquachromium(III) complexes in aqueous solutions

1988 ◽  
Vol 27 (6) ◽  
pp. 986-989 ◽  
Author(s):  
Hiroshi Ogino ◽  
Yoichi Abe ◽  
Masato Shoji
Keyword(s):  
Aqueous Solutions ◽  
Carbon Bonds ◽  
In Trans ◽  
Cleavage Reactions ◽  
Cis And Trans

The Stereospecific Synthesis of Neutral and Cationic Tertiary Phosphine Complexes of Platinum(II)

1974 ◽  
Vol 52 (4) ◽  
pp. 692-695 ◽  
Author(s):  
Keith R. Dixon ◽  
Kenneth C. Moss ◽  
Martin A. R. Smith
Keyword(s):  
Synthetic Methods ◽  
Stereospecific Synthesis ◽  
Tertiary Phosphine ◽  
Phosphine Complexes ◽  
Square Planar ◽  
Cleavage Reactions ◽  
Cis And Trans

Stereospecific synthetic methods for the preparation of complexes of the type cis- and trans[PtClL(PEt3)2]+ and cis-[PtClX(PEt3)2] have been developed. Preparations of complexes where L = py, PPh3, PClPh2, or P(OPh)3 and X = SCN, NO2, NO3, N3, NCO, or Br are described and it is shown that bridge cleavage reactions in square planar platinum(II) complexes occur with retention of stereo chemistry.

Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae

1991 ◽  
Vol 11 (11) ◽  
pp. 5710-5717
Author(s):  
E A Malone ◽  
C D Clark ◽  
A Chiang ◽  
F Winston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Copy Number ◽  
Cell Cycle Control ◽  
Control Point ◽  
Temperature Sensitive ◽  
Upstream Activating Sequence ◽  
In Trans ◽  
Cis And Trans ◽  
Insertion Mutations

SPT16 was previously identified as a high-copy-number suppressor of delta insertion mutations in the 5' regions of the HIS4 and LYS2 genes of Saccharomyces cerevisiae. We have constructed null mutations in the SPT16 gene and have demonstrated that it is essential for growth. Temperature-sensitive-lethality spt16 alleles have been isolated and shown to be pleiotropic; at a temperature permissive for growth, spt16 mutations suppress delta insertion mutations, a deletion of the SUC2 upstream activating sequence, and mutations in trans-acting genes required for both SUC2 and Ty expression. In addition, SPT16 is identical to CDC68, a gene previously shown to be required for passage through the cell cycle control point START. However, at least some transcriptional effects caused by spt16 mutations are independent of arrest at START. These results and those in the accompanying paper (A. Rowley, R. A. Singer, and G. C. Johnston, Mol. Cell. Biol. 11:5718-5726, 1991) indicate that SPT16/CDC68 is required for normal transcription of many loci in S. cerevisiae.

A silencer element from the alpha-globin gene inhibits expression of beta-like genes

1988 ◽  
Vol 8 (11) ◽  
pp. 5047-5051
Author(s):  
G F Atweh ◽  
J M Liu ◽  
H E Brickner ◽  
X X Zhu
Keyword(s):  
Globin Gene ◽  
Expression System ◽  
Globin Genes ◽  
Beta Globin ◽  
Base Pair Fragment ◽  
Alpha Globin ◽  
In Trans ◽  
Cis And Trans ◽  
In Cis ◽  
Silencer Element

We have studied the cis and trans interactions of the alpha- and beta-globin genes in a transient expression system. We found that the alpha-globin gene inhibited beta-globin expression in cis but not in trans. The silencer element responsible for this inhibition was localized to a 259-base-pair fragment at the 5' end of the alpha-globin gene.

Morphology and radical reactions of Cu(II) and Co(II) sulfonated phthalocyanines covalently linked to poly(ethyleneamide)

2010 ◽  
Vol 14 (01) ◽  
pp. 69-80 ◽  
Author(s):  
Gustavo T. Ruiz ◽  
Alexander G. Lappin ◽  
Guillermo Ferraudi
Keyword(s):  
Aqueous Solutions ◽  
Redox Reactions ◽  
Radical Reactions ◽  
Stable Bundles ◽  
Carbon Bonds ◽  
Amine Groups ◽  
Covalently Linked ◽  
The Stability ◽  
Sulfonated Phthalocyanines

The tetrasulfonated Cu II( tspc )4-, tspc = 4,4′,4″,4‴-phthalocyaninetetrasulfonate, and the trisulfonated Co II( trspc )3-, trspc = n , n ′, n ″-phthalocyanine-trisulfonate and n, n′ and n″ indicate the sulfonated positions of the various isomers, were covalently linked to a polyethyleneimine, M n ~ 10000 and M w ~ 25000, backbone. A fraction of the amine groups in a strand was converted to Cu II pc (- SO 3)3(- SO 2 N <)\3- or Co II pc (- SO 3)2(- SO 2 N <)\2- pendants and the remaining were acetylated. Strands of the polymers, poly(K3CuIItspc) and poly(K2CoIItrspc) , formed spherical bundles with diameters ~1000 nm for poly(K3CuIItspc) and ~100 nm for poly(K2CoIItrspc) . The stability of the bundles is metal-dependent. Poly(K2CoIItrspc) forms the most stable bundles with respect to hydrolytic and redox reactions. ESR and optical spectroscopies as well as reactions of the pendants with pulse radiolytically generated radicals, namely e - sol , C•H2OH, (CH3)2COHC•H2 , CO 2•-, N 3• and SO 4•-, revealed a distribution of pendants between isolated and aggregated forms in a bundle. The reduction of the Cu(II) to Cu(I) in aqueous solutions of the poly(K3CuIItspc) was associated with pendants present in a mostly hydrophobic environment. Unstable phthalocyanine pendants with CoIII -carbon bonds are formed when CoIIpc(-SO3)2(-SO2N<)\2- reacted with C -centered radicals, ( CH3)2COHC•H2 and C•H2OH . The species with CoIII -carbon bonds were precursors to the formation of CoIpc(-SO3)2(-SO2N<)\3- pendants. The redox reactions of the pendants in these polymers are compared with those of K4CuIItspc and K3CoIItrspc .

Comparison of cis and trans-Platinum Mononucleobase Compounds with DNA and Protein Models

2008 ◽  
Vol 61 (9) ◽  
pp. 694 ◽  
Author(s):  
Joseph V. Strukl ◽  
Queite A. de Paula ◽  
Xiaohong Yang ◽  
Yun Qu ◽  
Nicholas P. Farrell
Keyword(s):  
Nmr Spectroscopy ◽  
Antiviral Activity ◽  
Aqueous Solutions ◽  
Guanosine Monophosphate ◽  
Molar Ratio ◽  
Trans Isomers ◽  
Specific Proteins ◽  
Low Cytotoxicity ◽  
Cis And Trans ◽  
Protein Models

Reactions of 5′-guanosine monophosphate (5′-GMP) and N-acetylmethionine (N-ac-l-Met) with the mononucleobase compounds, cis-[PtCl(L)n(9-EtGH)]+ (L = NH3, 4-pic, n = 2; L = en, n = 1) in a 1:1 molar ratio have been studied in aqueous solutions at pH 7.3 using 1H and 195Pt NMR spectroscopy. There is a high kinetic preference for sulfur over nitrogen binding. These results are compared with the trans isomers. Based on low cytotoxicity and a high sulfur/nitrogen preference the cis isomers may also present suitable features for antiviral activity through interaction with specific proteins.

Cleavage of carbon–carbon bonds by radical reactions

2019 ◽  
Vol 48 (9) ◽  
pp. 2615-2656 ◽  
Author(s):  
Paramasivam Sivaguru ◽  
Zikun Wang ◽  
Giuseppe Zanoni ◽  
Xihe Bi
Keyword(s):  
Bond Cleavage ◽  
Radical Reactions ◽  
Carbon Bond ◽  
Carbon Carbon Bond ◽  
Carbon Carbon Bonds ◽  
Carbon Bonds ◽  
Cleavage Reactions

This review provides insights into the in situ generated radicals triggered carbon–carbon bond cleavage reactions.

scholarly journals The Yeast Casein Kinase Yck3p Is Palmitoylated, then Sorted to the Vacuolar Membrane with AP-3-dependent Recognition of a YXXϕ Adaptin Sorting Signal

2004 ◽  
Vol 15 (3) ◽  
pp. 1397-1406 ◽  
Author(s):  
Beimeng Sun ◽  
Linyi Chen ◽  
Wei Cao ◽  
Amy F. Roth ◽  
Nicholas G. Davis
Keyword(s):  
Plasma Membrane ◽  
Mammalian Cells ◽  
Casein Kinase ◽  
Vacuolar Membrane ◽  
Sorting Signal ◽  
Casein Kinases ◽  
In Trans ◽  
Vacuolar Sorting ◽  
Cis And Trans ◽  
In Cis

Our previous work found the two yeast plasma membrane-localized casein kinases Yck1p and Yck2p to be palmitoylated on C-terminal Cys-Cys sequences by the palmitoyl transferase Akr1p. The present work examines a third casein kinase, Yck3p, which ends with the C-terminal sequence Cys-Cys-Cys-Cys-Phe-Cys-Cys-Cys. Yck3p is palmitoylated and localized to the vacuolar membrane. While the C-terminal cysteines are required for this palmitoylation, Akr1p is not. Palmitoylation requires the C-terminal Yck3p residues 463-524, whereas information for vacuolar sorting maps to the 409-462 interval. Vacuolar sorting is disrupted in cis through deletion of the 409-462 sequences and in trans through mutation of the AP-3 adaptin complex; both cis- and trans-mutations result in Yck3p missorting to the plasma membrane. This missorted Yck3p restores 37°C viability to yck1Δ yck2-ts cells. yck1Δ yck2-ts suppressor mutations isolated within the YCK3 gene identify the Yck3p vacuolar sorting signal—the tetrapeptide YDSI, a perfect fit to the YXXϕ adaptin-binding consensus. Although YXXϕ signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the first signal of this class to be identified in yeast.

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