Gold(I)-Catalyzed Rearrangement of Propargyl Benzyl Ethers: A Practical Method for the Generation and in Situ Transformation of Substituted Allenes

2010 ◽  
Vol 132 (21) ◽  
pp. 7294-7296 ◽  
Author(s):  
Benoit Bolte ◽  
Yann Odabachian ◽  
Fabien Gagosz
Keyword(s):  
Practical Method ◽  
Benzyl Ethers

ChemInform Abstract: Gold(I)-Catalyzed Rearrangement of Propargyl Benzyl Ethers: A Practical Method for the Generation and in situ Transformation of Substituted Allenes.

ChemInform ◽  
2010 ◽  
Vol 41 (39) ◽  
pp. no-no
Author(s):  
Benoit Bolte ◽  
Yann Odabachian ◽  
Fabien Gagosz
Keyword(s):  
Practical Method ◽  
Benzyl Ethers

Benzylation of arenes with benzyl ethers promoted by the in situ prepared superacid BF3–H2O

2014 ◽  
Vol 16 (6) ◽  
pp. 2976 ◽  
Author(s):  
Yu Li ◽  
Yan Xiong ◽  
Xueming Li ◽  
Xuege Ling ◽  
Ruofeng Huang ◽  
...  
Keyword(s):  
Benzyl Ethers

scholarly journals Nanopore-mediated protein delivery enabling three-color single-molecule tracking in living cells

2021 ◽  
Vol 118 (5) ◽  
pp. e2012229118
Author(s):  
Zhongwen Chen ◽  
Yuhong Cao ◽  
Chun-Wei Lin ◽  
Steven Alvarez ◽  
Dongmyung Oh ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Delivery ◽  
Chemical Properties ◽  
Practical Method ◽  
Living Cells ◽  
Egfr Signaling ◽  
Single Molecule Tracking ◽  
Ras Activation ◽  
And Chemical Properties

Multicolor single-molecule tracking (SMT) provides a powerful tool to mechanistically probe molecular interactions in living cells. However, because of the limitations in the optical and chemical properties of currently available fluorophores and the multiprotein labeling strategies, intracellular multicolor SMT remains challenging for general research studies. Here, we introduce a practical method employing a nanopore-electroporation (NanoEP) technique to deliver multiple organic dye-labeled proteins into living cells for imaging. It can be easily expanded to three channels in commercial microscopes or be combined with other in situ labeling methods. Utilizing NanoEP, we demonstrate three-color SMT for both cytosolic and membrane proteins. Specifically, we simultaneously monitored single-molecule events downstream of EGFR signaling pathways in living cells. The results provide detailed resolution of the spatial localization and dynamics of Grb2 and SOS recruitment to activated EGFR along with the resultant Ras activation.

scholarly journals Practical Method to Screen Contaminated Holograms of Flocs Using Light Intensity

2021 ◽  
Vol 8 ◽  
Author(s):  
Sun Min Choi ◽  
Jun Young Seo ◽  
Guan-hong Lee ◽  
Xiaoteng Shen ◽  
Ho Kyung Ha
Keyword(s):  
Light Intensity ◽  
Practical Method ◽  
Accurate Estimation ◽  
Cohesive Sediments ◽  
Degree Of Contamination ◽  
Particle Properties ◽  
And Behavior ◽  
Biological Organisms ◽  
Number Of Particles

Submergible digital holographic camera can measure the in situ size and shape of suspended particles, such as complex flocs and biological organisms, without disturbance. As the number of particles in the water column increases, overlapping concentric rings (interference patterns) can contaminate the holographic images. Using light intensity (LI), this study proposes a practical method to assess the degree of contamination and screen out contaminated images. The outcomes from image processing support that LI normalized on a gray scale of 0 (black) to 255 (white) can be a reliable criterion for defining the contamination boundary. Results found that as LI increased, the shape of the particle size distribution shifted from a positively skewed to a normal distribution. When LI was lower than approximately 80, owing to the distortion of particle properties, the settling velocities derived from the contaminated holograms with mosaic patterns were underestimated compared to those from the uncontaminated holograms. The proposed method can contribute to a more accurate estimation of the transport and behavior of cohesive sediments in shallow estuarine environments.

Catalyst-free formation of 1,4-diketones by addition of silyl enolates to oxyallyl zwitterions in situ generated from α-haloketones

RSC Advances ◽  
2015 ◽  
Vol 5 (83) ◽  
pp. 67901-67908 ◽  
Author(s):  
Juan Luo ◽  
Qihua Jiang ◽  
Hao Chen ◽  
Qiang Tang
Keyword(s):  
Room Temperature ◽  
Practical Method ◽  
Direct Coupling ◽  
Catalyst Free

Here we report an efficient and practical method for the preparation of 1,4-diketones by direct coupling of α-haloketones with silyl enolates at room temperature. No catalysts were required in our protocol.

scholarly journals In Situ Electrophilic Activation of Hydrogen Peroxide for Catalytic Asymmetric α-Hydroxylation of 3-Substituted Oxindoles

Synlett ◽  
2017 ◽  
Vol 28 (11) ◽  
pp. 1291-1294 ◽  
Author(s):  
Takashi Ooi ◽  
Kohsuke Ohmatsu ◽  
Yuichiro Ando
Keyword(s):  
Hydrogen Peroxide ◽  
Phase Transfer ◽  
Practical Method ◽  
Optically Active ◽  
Aqueous Hydrogen Peroxide ◽  
Bond Forming ◽  
Electrophilic Activation ◽  
Oxygen Bond ◽  
Activation Of Hydrogen Peroxide

Peroxy trichloroacetimidic acid, in situ generated from aqueous hydrogen peroxide and trichloroacetonitrile, was found to act as a competent electrophilic oxygenating agent for the direct α-hydroxylation of oxindoles. The use of chiral 1,2,3-triazolium salt as a phase-transfer catalyst enabled rigorous absolute stereocontrol in the carbon–oxygen bond-forming reaction. The present study provides a new, yet practical method for straightforward access to optically active α-hydroxycarbonyl compounds.

scholarly journals An Approach for In Situ Rapid Detection of Deep-Sea Aromatic Amino Acids Using Laser-Induced Fluorescence

Sensors ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 1330
Author(s):  
Ranran Du ◽  
Dingtian Yang ◽  
Guangjia Jiang ◽  
Youren Song ◽  
Xiaoqing Yin
Keyword(s):  
Amino Acids ◽  
Deep Sea ◽  
Rapid Detection ◽  
Aromatic Amino Acids ◽  
Practical Method ◽  
Laser Induced Fluorescence ◽  
Carbon Cycles ◽  
Material Circulation ◽  
Almost All

Amino acids are the material basis of almost all life activities. An improved understanding of the source, state, and cycle of amino acids is essential for determining the energy flow and material circulation of marine ecosystems. In the present study, an in situ rapid detection method of ultraviolet (UV; 266 nm) laser-induced fluorescence (LIF) technology was used to detect three natural, aromatic amino acids in the seawater. The laser-induced fluorescence peaks of aromatic amino acids tryptophan, tyrosine, and phenylalanine were located at 350 nm, 300 nm, and 280 nm, respectively. High, linear correlations between the concentrations of the aromatic amino acids and the fluorescence peak heights were observed, and the lowest detectable concentrations of tryptophan, tyrosine, and phenylalanine were 4.70 × 10−9 mol/L, 2.76 × 10−8 mol/L, and 6.05 × 10−7 mol/L, respectively, which allowed us to quantify their concentrations by using laser-induced fluorescence. This paper not only provides a practical method for the detection of aromatic amino acids in seawater, but a new means to further understand the biogeochemical processes of carbon cycles in the deep sea.

scholarly journals Copper-Catalyzed Benzylic C–H Coupling with Alcohols via Radical Relay

2019 ◽  
Author(s):  
Huayou Hu ◽  
Si-Jie Chen ◽  
Shane Krska ◽  
Shannon Stahl
Keyword(s):  
Copper Catalyst ◽  
Three Dimensional ◽  
Cross Coupling ◽  
Coupling Reactions ◽  
Cross Coupling Reactions ◽  
Selective Functionalization ◽  
Modern Chemical ◽  
Benzyl Ethers ◽  
Site Selective

Cross coupling reactions enable rapid convergent synthesis of diverse molecules and provide the foundation for modern chemical synthesis. The most widely used methods employ sp2-hybridized coupling partners, such as aryl halides or related pre-functionalized substrates. Here, we demonstrate copper-catalyzed oxidative cross-coupling of benzylic C–H bonds with alcohols to afford benzyl ethers, enabled by mechanistic insights that led to a novel reductant-based strategy for in situ regeneration of the active copper catalyst. The reactions employ the C–H substrate as the limiting reagent and exhibit broad scope with respect to both substrate partners. This approach to direct site-selective functionalization of sp3 C–H bonds provides the basis for efficient three-dimensional diversification of organic molecules and should find widespread utility in organic synthesis, particularly for medicinal chemistry applications.

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