scholarly journals Practical Method to Screen Contaminated Holograms of Flocs Using Light Intensity

2021 ◽  
Vol 8 ◽  
Author(s):  
Sun Min Choi ◽  
Jun Young Seo ◽  
Guan-hong Lee ◽  
Xiaoteng Shen ◽  
Ho Kyung Ha

Submergible digital holographic camera can measure the in situ size and shape of suspended particles, such as complex flocs and biological organisms, without disturbance. As the number of particles in the water column increases, overlapping concentric rings (interference patterns) can contaminate the holographic images. Using light intensity (LI), this study proposes a practical method to assess the degree of contamination and screen out contaminated images. The outcomes from image processing support that LI normalized on a gray scale of 0 (black) to 255 (white) can be a reliable criterion for defining the contamination boundary. Results found that as LI increased, the shape of the particle size distribution shifted from a positively skewed to a normal distribution. When LI was lower than approximately 80, owing to the distortion of particle properties, the settling velocities derived from the contaminated holograms with mosaic patterns were underestimated compared to those from the uncontaminated holograms. The proposed method can contribute to a more accurate estimation of the transport and behavior of cohesive sediments in shallow estuarine environments.

2021 ◽  
Vol 91 (4) ◽  
pp. 390-398
Author(s):  
Yuhao Li ◽  
Bruce R. Sutherland ◽  
Murray K. Gingras ◽  
George W. Owttrim ◽  
Kurt O. Konhauser

ABSTRACT Sedimentation velocities of various chemical sediments are typically calculated using Stokes's law. However, applying it to chemical sediments that form in situ in the water column is not ideal because the particle properties do not fulfill many of the assumptions underpinning the applicability of Stokes' law. As a consequence, it has been difficult to predict the sedimentation rate of ancient chemical sediments, such as Precambrian banded iron formations (BIF), because their primary sediments likely comprised aggregates of ferric hydroxides, such as ferrihydrite [Fe(OH)3], and marine bacterial biomass, including cyanobacteria. In this work we use a new experimental method to address the mechanisms by which primary BIF sediment, formed by the oxidation of dissolved Fe(II) by O2 and simultaneously incubated with cyanobacterium Synechococcus sp., were deposited to the Archean ocean. Specifically, we formed the aggregates in situ over a wide range of initial pH and Fe(II) concentrations, continuously recorded the entire settling processes of aggregates under each condition, and then processed the data in MATLAB according to different settling mechanisms. Our results demonstrate that ferrihydrite–cyanobacteria aggregates settled to the ocean floor either through the formation of uniformly descending concentration fronts or through convective plumes. The sedimentation mechanism depended on both initial Fe(II) concentration and the pH. Correspondingly, two algorithms were developed to characterize the sedimentation velocity. These algorithms tracked the alteration of light intensity from low to high as sediments descended from an initially homogeneous state through a water tank, and as well calculated the average light intensity over time, from which vertical time series were constructed allowing calculation of the sedimentation velocity. Our method not only provides an accurate estimation of the in situ sedimentation velocity of cell–mineral aggregates, but also provides new insights into the physical mechanisms by which the primary sediments composing BIF were deposited.


Author(s):  
Miss Payal W. Paratpure

Tracking of public bus location requires a GPS device to be installed, and lots of bus operators in developing countries don't have such an answer in situ to supply an accurate estimation of bus time of arrival (ETA). Without ETA information, it's very difficult for the overall public to plan their journey effectively. In this paper, implementation of an innovative IOT solution to trace the real time location of buses without requiring the deployment of a GPS device is discussed. It uses Bluetooth Low Energy (BLE) proximity beacon to trace the journey of a bus by deploying an Estimate location beacon on the bus. BLE detection devices (Raspberry Pi 4) are installed at selected bus stops along the path to detect the arrival of buses. Once detected, the situation of the bus is submitted to a cloud server to compute the bus ETAs. A field trial is currently being conducted in Johor, Malaysia together with an area bus operator on one single path. Our test results showed that the detection of BLE beacons is extremely accurate and it's feasible to trace the situation of buses without employing a GPS device during a cost-effective way.


2013 ◽  
Vol 17 (12) ◽  
pp. 4925-4939 ◽  
Author(s):  
L. Seoane ◽  
G. Ramillien ◽  
F. Frappart ◽  
M. Leblanc

Abstract. Time series of regional 2° × 2° Gravity Recovery and Climate Experiment (GRACE) solutions have been computed from 2003 to 2011 with a 10-day resolution by using an energy integral method over Australia (112° E–156° E; 44° S–10° S). This approach uses the dynamical orbit analysis of GRACE Level 1 measurements, and specially accurate along-track K-band range rate (KBRR) residuals with a 1 μm s−1 level of errors, to estimate the total water mass over continental regions. The advantages of regional solutions are a significant reduction of GRACE aliasing errors (i.e. north–south stripes) providing a more accurate estimation of water mass balance for hydrological applications. In this paper, the validation of these regional solutions over Australia is presented, as well as their ability to describe water mass change as a response of climate forcings such as El Niño. Principal component analysis of GRACE-derived total water storage (TWS) maps shows spatial and temporal patterns that are consistent with independent data sets (e.g. rainfall, climate index and in situ observations). Regional TWS maps show higher spatial correlations with in situ water table measurements over Murray–Darling drainage basin (80–90%), and they offer a better localization of hydrological structures than classical GRACE global solutions (i.e. Level 2 Groupe de Recherche en Géodésie Spatiale (GRGS)) products and 400 km independent component analysis solutions as a linear combination of GRACE solutions provided by different centers.


Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3353
Author(s):  
Jo Ann Chew ◽  
Tina Widowski ◽  
Eugenia Herwig ◽  
Tory Shynkaruk ◽  
Karen Schwean-Lardner

The effects of light intensity (L) are not well studied in pullets. Our research objective was to study the effect of L on navigational success, behavior, and welfare of two pullet strains (S). In two repeated trials, a 3 × 2 × 4 factorial arrangement tested three L (10, 30, 50 lux) and two S (Lohmann Brown-Lite (LB), LSL-Lite (LW)) at four ages. One thousand eight hundred pullets/S (0–16 wk) were randomly assigned to floor pens within light-tight rooms (three pens/S/room, four rooms/L) containing four parallel perches and a ramp. Data collection included jumping frequency and success (24h continuous sampling), novel object tests (fear), heterophil to lymphocyte (H/L) ratios (stress), and behavior (instantaneous scan sampling) during photoperiods. L did not affect injurious behavior, fear, or H/L. Pullets reared at 50 lux spent more time preening than at 10 lux. Pullets reared at 10 lux spent more time wall pecking than at 50 lux. Time spent standing and preening and total number and accuracy of jumping increased with age. Pullets reared at 30 lux had higher jumping frequency than at 10 lux; accuracy was not affected. LW jumped more than LB, but with similar success. LB spent more time exploring and scored higher in the fear and stress assessments, suggesting S differences.


2021 ◽  
Author(s):  
Jutta Kesti ◽  
John Backman ◽  
Ewan James O'Connor ◽  
Anne Hirsikko ◽  
Eija Asmi ◽  
...  

Abstract. Aerosol particles play an important in role in the microphysics of clouds and hence on their likelihood to precipitate. In the changing climate already dry areas such as the United Arab Emirates (UAE) are predicted to become even drier. Comprehensive observations of the daily and seasonal variation in aerosol particle properties in such locations are required reducing the uncertainty in such predictions. We analyse observations from a one-year measurement campaign at a background location in the United Arab Emirates to investigate the properties of aerosol particles in this region, study the impact of boundary layer mixing on background aerosol particle properties measured at the surface and study the temporal evolution of the aerosol particle cloud formation potential in the region. We used in-situ aerosol particle measurements to characterise the aerosol particle composition, size, number and cloud condensation nuclei (CCN) properties, in-situ SO2 measurements as an anthropogenic signature and a long-range scanning Doppler lidar to provide vertical profiles of the horizontal wind and turbulent properties to monitor the evolution of the boundary layer. Anthropogenic sulphate dominated the aerosol particle mass composition in this location. There was a clear diurnal cycle in the surface wind direction, which had a strong impact on aerosol particle total number concentration, SO2 concentration and black carbon mass concentration. Local sources were the predominant source of black carbon, as concentrations clearly depended on the presence of turbulent mixing, with much higher values during calm nights. The measured concentrations of SO2, instead, were highly dependent on the surface wind direction as well as on the depth of the boundary layer when entrainment from the advected elevated layers occurred. The wind direction at the surface or of the elevated layer suggests that the cities of Dubai, Abu Dhabi and other coastal conurbations were the remote sources of SO2. We observed new aerosol particle formation events almost every day (on four days out of five on average). Calm nights had the highest CCN number concentrations and lowest κ values and activation fractions. We did not observe any clear dependence of CCN number concentration and κ parameter on the height of the daytime boundary layer, whereas the activation fraction did show a slight increase with increasing boundary layer height, due to the change in the shape of the aerosol particle size distribution where the relative portion of larger aerosol particles increased with increasing boundary layer height. We believe that this indicates that size is more important than chemistry for aerosol particle CCN activation at this site. The combination of instrumentation used in this campaign enabled us to identify periods when anthropogenic pollution from remote sources that had been transported in elevated layers was present, and had been mixed down to the surface in the growing boundary layer.


1972 ◽  
Vol 50 (12) ◽  
pp. 2673-2682 ◽  
Author(s):  
William H. Harvey ◽  
James D. Caponetti

Intact, set III, cinnamon fern cataphyll and frond primordia, which were shown to have no predisposition to fertility in situ, produced sporangia when excised and cultured under sterile conditions in Knudson's medium supplemented with various levels of sucrose and maintained on 11 different regimens of light, darkness, and temperature for 10 weeks. Increasing levels of sucrose resulted in increased fertility under all environmental conditions, but the highest percentage of fertility was obtained under conditions of continuous dark at 26 °C. As the length of the light phase of the photoperiods decreased, a progressive increase in induction of fertile leaves was observed, suggesting that periods of long light exposure are inhibitory to the initiation of sporangia. Conversely, as the light intensity was increased, an inhibition of sporophyll differentiation occurred. Sporangia excised from dark-induced sporophylls and cultured in the light produced viable spores which germinated yielding haploid gametophytes that ultimately produced sporophytes.


2017 ◽  
Vol 48 (3) ◽  
pp. 616-633 ◽  
Author(s):  
G. Farina ◽  
S. Alvisi ◽  
M. Franchini

This paper presents a procedure for estimating discharge in a river cross-section based on the combined use of dimensionless isovels and point velocity measurements. Specifically, taking the Biot–Savart law on the magnetic field induced by an electric current in a wire as their basis as already done by other researchers, the authors propose a new formulation of the relationship characterizing the effect of the wetted perimeter on the range of velocities in a cross-section in order to take explicit account of roughness, expressed by means of Manning's coefficient. Once appropriately nondimensionalized, the isoeffect contours can be read as dimensionless isovels. Assuming in situ velocity measurements are available, discharge at a cross-section can be computed using two different methods. The proposed procedure was applied to six case studies characterized by river cross-sections which differed greatly from one another. The results show that the two methods proposed for estimating discharge lead to equivalent outcomes, and in all the cases the procedure as a whole enables a sufficiently accurate estimation of discharge, even when it is based on a limited number of velocity measurements or on the measurement of maximum surface-water velocity alone.


2020 ◽  
Vol 105 (3) ◽  
pp. 307-318 ◽  
Author(s):  
Benjamin M. Urann ◽  
Véronique Le Roux ◽  
Timm John ◽  
Grace M. Beaudoin ◽  
Jaime D. Barnes

Abstract We present in situ secondary ion mass spectrometry (SIMS) and electron microprobe analyses of coexisting garnet, omphacite, phengite, amphibole, and apatite, combined with pyrohydrolysis bulk-rock analyses to constrain the distribution, abundance, and behavior of halogens (F and Cl) in six MORB-like eclogites from the Raspas Complex (Southern Ecuador). In all cases concerning lattice-hosted halogens, F compatibility decreases from apatite (1.47–3.25 wt%), to amphibole (563–4727 μg/g), phengite (610–1822 μg/g), omphacite (6.5–54.1 μg/g), and garnet (1.7–8.9 μg/g). The relative compatibility of Cl in the assemblage is greatest for apatite (192–515 μg/g), followed by amphibole (0.64–82.7 μg/g), phengite (1.2–2.1 μg/g), omphacite (<0.05–1.0 μg/g), and garnet (<0.05 μg/g). Congruence between SIMS-reconstructed F bulk abundances and yield-corrected bulk pyrohydrolysis analyses indicates that F is primarily hosted within the crystal lattice of eclogitic minerals. However, SIMS-reconstructed Cl abundances are a factor of five lower, on average, than pyrohydrolysis-derived bulk concentrations. This discrepancy results from the contribution of fluid inclusions, which may host at least 80% of the bulk rock Cl. The combination of SIMS and pyrohydrolysis is highly complementary. Whereas SIMS is well suited to determine bulk F abundances, pyrohydrolysis better quantifies bulk Cl concentrations, which include the contribution of fluid inclusion-hosted Cl. Raspas eclogites contain 145–258 μg/g F and at least 7–11 μg/g Cl. We estimate that ~95% of F is retained in the slab through eclogitization and returned to the upper mantle during subduction, whereas at least 95% of subducted Cl is removed from the rock by the time the slab equilibrates at eclogite facies conditions. Our calculations provide further evidence for the fractionation of F from Cl during high-pressure metamorphism in subduction zones. Although the HIMU (high U/Pb) mantle source (dehydrated oceanic crust) is often associated with enrichments in Cl/K and F/Nd, Raspas eclogites show relatively low halogen ratios identical within uncertainty to depleted MORB mantle (DMM). Thus, the observed halogen enrichments in HIMU ocean island basalts require either further fractionation during mantle processing or recycling of a halogen-enriched carrier lithology such as serpentinite into the mantle.


1973 ◽  
Vol 30 (5) ◽  
pp. 708-711 ◽  
Author(s):  
F. J. Ward ◽  
Masami Nakanishi

For an in situ experiment conducted in Shiozu Bay, Lake Biwa, Japan, primary productivity estimates from liquid scintillation radioactivity counts of wet algae were generally higher than those from Geiger–Müller radioactivity counts of desiccated algae. Values at 0 m were similar, the G–M estimate at 0.5 m was 10% higher, but from 3 to 13 m the liquid scintillation values ranged from 11 to 33% higher than G–M estimates. The 20-m estimates were low and similar. Differences were caused primarily by 14C losses during desiccation prior to G–M counting. Increasing loss rates between 0.5 and 3.0 m may have been caused by decreasing light intensity. On the basis of surface area, the estimate from liquid scintillation data was 27% greater than that obtained from G–M data.


Minerals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 433
Author(s):  
Annemerel Mol ◽  
Renata van der Weijden ◽  
Johannes Klok ◽  
Cees Buisman

In the biodesulfurization (BD) process under halo-alkaline conditions, toxic hydrogen sulfide is oxidized to elemental sulfur by a mixed culture of sulfide oxidizing bacteria to clean biogas. The resulting sulfur is recovered by gravitational settling and can be used as raw material in various industries. However, if the sulfur particles do not settle, it will lead to operational difficulties. In this study, we investigated the properties of sulfur formed in five industrial BD facilities. Sulfur particles from all samples showed large differences in terms of shape, size, and settleability. Both single crystals (often bipyramidal) and aggregates thereof were observed with light and scanning electron microscopy. The small, non-settled particles account for at least 13.6% of the total number of particles and consists of small individual particles with a median of 0.3 µm. This is undesirable, because those particles cannot be removed from the BD facility by gravitational settling and lead to operational interruption. The particles with good settling properties are aggregates (5–20 µm) or large single crystals (20 µm). We provide hypotheses as to how the differences in sulfur particle properties might have occurred. These findings provide a basis for understanding the relation between sulfur particle properties and formation mechanisms.


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