Isolation of anteiso-C17, iso-C17, iso-C16, and iso-C15 Bacillomycin D from Bacillus amyloliquefaciens SD-32 and Their Antifungal Activities against Plant Pathogens

AbstractThe success of Bacillus amyloliquefaciens as a biological control agent relies on its ability to outgrow plant pathogens. It is also thought to interact with its plant host by inducing systemic resistance. In this study, the ability of B. amyloliquefaciens MBI600 to elicit defense (or other) responses in tomato seedlings and plants was assessed upon the expression of marker genes and transcriptomic analysis. Spray application of Serifel, a commercial formulation of MBI600, induced responses in a dose-dependent manner. Low dosage primed plant defense by activation of SA-responsive genes. Suggested dosage induced defense by mediating synergistic cross-talk between JA/ET and SA-signaling. Saturation of tomato roots or leaves with MBI600 elicitors activated JA/ET signaling at the expense of SA-mediated responses. The complex signaling network that is implicated in MBI600-tomato seedling interactions was mapped. MBI600 and flg22 (a bacterial flagellin peptide) elicitors induced, in a similar manner, biotic and abiotic stress responses by the coordinated activation of genes involved in JA/ET biosynthesis as well as hormone and redox signaling. This is the first study to suggest the activation of plant defense following the application of a commercial microbial formulation under conditions of greenhouse crop production.

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Knockout of rapC Improves the Bacillomycin D Yield Based on De Novo Genome Sequencing of Bacillus amyloliquefaciens fmbJ

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.8b00418 ◽

2018 ◽

Vol 66 (17) ◽

pp. 4422-4430 ◽

Cited By ~ 5

Author(s):

Jing Sun ◽

Shiquan Qian ◽

Jing Lu ◽

Yanan Liu ◽

Fengxia Lu ◽

...

Keyword(s):

Genome Sequencing ◽

Bacillus Amyloliquefaciens ◽

De Novo ◽

Bacillomycin D

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Bacillomycin D Produced by Bacillus amyloliquefaciens Is Involved in the Antagonistic Interaction with the Plant-Pathogenic Fungus Fusarium graminearum

Applied and Environmental Microbiology ◽

10.1128/aem.01075-17 ◽

2017 ◽

Vol 83 (19) ◽

Cited By ~ 57

Author(s):

Qin Gu ◽

Yang Yang ◽

Qiming Yuan ◽

Guangming Shi ◽

Liming Wu ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Fusarium Graminearum ◽

Bacillus Amyloliquefaciens ◽

Morphological Changes ◽

Reactive Oxygen ◽

Oxygen Species ◽

Strong Activity ◽

Content Type ◽

Mitogen Activated Protein ◽

Bacillomycin D

ABSTRACT Fusarium graminearum (teleomorph: Ascomycota, Hypocreales, Gibberella, Gibberella zeae) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. Controlling this toxin-producing pathogen is a significant challenge. In the present study, the commercially available strain Bacillus amyloliquefaciens (Bacteria, Firmicutes, Bacillales, Bacillus) FZB42 showed strong activity against F. graminearum. The lipopeptide bacillomycin D, produced by FZB42, was shown to contribute to the antifungal activity. Purified bacillomycin D showed strong activity against F. graminearum, and its 50% effective concentration was determined to be approximately 30 μg/ml. Analyses using scanning and transmission electron microscopy revealed that bacillomycin D caused morphological changes in the plasma membranes and cell walls of F. graminearum hyphae and conidia. Fluorescence microscopy combined with different dyes showed that bacillomycin D induced the accumulation of reactive oxygen species and caused cell death in F. graminearum hyphae and conidia. F. graminearum secondary metabolism also responded to bacillomycin D challenge, by increasing the production of deoxynivalenol. Biological control experiments demonstrated that bacillomycin D exerted good control of F. graminearum on corn silks, wheat seedlings, and wheat heads. In response to bacillomycin D, F. graminearum genes involved in scavenging reactive oxygen species were downregulated, whereas genes involved in the synthesis of deoxynivalenol were upregulated. Phosphorylation of MGV1 and HOG1, the mitogen-activated protein kinases of F. graminearum, was increased in response to bacillomycin D. Taken together, these findings reveal the mechanism of the antifungal action of bacillomycin D. IMPORTANCE Biological control of plant disease caused by Fusarium graminearum is desirable. Bacillus amyloliquefaciens FZB42 is a representative of the biocontrol bacterial strains. In this work, the lipopeptide bacillomycin D, produced by FZB42, showed strong fungicidal activity against F. graminearum. Bacillomycin D caused morphological changes in the plasma membrane and cell wall of F. graminearum, induced accumulation of reactive oxygen species, and ultimately caused cell death in F. graminearum. Interestingly, when F. graminearum was challenged with bacillomycin D, the deoxynivalenol production, gene expression, mitogen-activated protein kinase phosphorylation, and pathogenicity of F. graminearum were significantly altered. These findings clarified the mechanisms of the activity of bacillomycin D against F. graminearum and highlighted the potential of B. amyloliquefaciens FZB42 as a biocontrol agent against F. graminearum.

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Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of Bacillus methylotrophicus

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.000226 ◽

2015 ◽

Vol 65 (Pt_7) ◽

pp. 2104-2109 ◽

Cited By ~ 48

Author(s):

Christopher A. Dunlap ◽

Soo-Jin Kim ◽

Soon-Wo Kwon ◽

Alejandro P. Rooney

Keyword(s):

Bacillus Amyloliquefaciens ◽

Type Strain ◽

Plant Pathogens ◽

Phylogenetic Analyses ◽

Draft Genome ◽

Genomic Analysis ◽

Comparative Genomic ◽

Phylogenomic Analysis ◽

Growth Promoters ◽

Bacillus Methylotrophicus

The rhizosphere-isolated bacteria belonging to the Bacillus amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. These properties have made these strains the focus of commercial interest. Here, we present the draft genome sequence of B. methylotrophicus KACC 13105T ( = CBMB205T). Comparative genomic analysis showed only minor differences between this strain and the genome of the B. amyloliquefaciens subsp. plantarum type strain, with the genomes sharing approximately 95 % of the same genes. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar. In fact, our results show that the type strain of B. amyloliquefaciens subsp. plantarum FZB42T ( = DSM 23117T = BGSC 10A6T) does not cluster with other members of the B. amyloliquefaciens taxon. Instead, it clusters well within a clade of strains that are assigned to B. methylotrophicus, including the type strain of that species. Therefore, we propose that the subspecies B. amyloliquefaciens subsp. plantarum should be reclassified as a later heterotypic synonym of B. methylotrophicus.

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Purification and characterisation of a fungicidal peptide from Bacillus amyloliquefaciens NCPSJ7

Czech Journal of Food Sciences ◽

10.17221/163/2016-cjfs ◽

2017 ◽

Vol 35 (No. 2) ◽

pp. 113-121

Author(s):

Wang Junhua ◽

Zhao Shuangzhi ◽

Qiu Jiying ◽

Zhou Qingxin ◽

Li Xiaoyong ◽

...

Keyword(s):

Electron Microscopy ◽

Molecular Mass ◽

Fusarium Oxysporum ◽

Bacillus Amyloliquefaciens ◽

Fungicidal Activity ◽

Pathogenic Fungi ◽

Antifungal Activities ◽

Minimum Fungicidal Concentration ◽

Antimicrobial Substances ◽

Wide Range

Bacillus amyloliquefaciens NCPSJ7 could secrete extracellular antimicrobial substances, showing potent antifungal activities. An active peptide AFP3 was isolated from the fermentation supernatant. After chromatography, the purified peptide was tested for the fungicidal activity, molecular mass, and stability. The results indicated that the peptide with a molecular mass of around 3.3 kDa, showed discernible inhibition of the pathogen Fusarium oxysporum f. sp. niveum with the minimum fungicidal concentration of 31 µg/ml. It also exhibited excellent inhibition of some representative pathogenic fungi at a low concentration. Moreover, the peptide remained active at a wide range of temperatures and pH. Ion Na<sup>+</sup> may even increase the antifungal activities. At the same time, the peptide could well tolerate the treatment with trypsin. Electron microscopy was used to investigate the effect of the peptide on the pathogens. The peptide inhibited the growth of pathogens by disrupting the integrity of the hyphal membranes, resulting in their lysis. The potent fungicidal activities and stability made the peptide be a candidate for a biopreservative.

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Antifungal Activity of β-Pinene-Based Hydronopyl Quaternary Ammonium Salts Against Phytopathogenic Fungi

Natural Product Communications ◽

10.1177/1934578x20948365 ◽

2020 ◽

Vol 15 (8) ◽

pp. 1934578X2094836

Author(s):

Xue Zhen Feng ◽

Zhuanquan Xiao ◽

Li Zhang ◽

Shengliang Liao ◽

Shangxing Chen ◽

...

Keyword(s):

Antifungal Activity ◽

Plant Pathogens ◽

Value Added ◽

Structure Activity Relationship ◽

Phytopathogenic Fungi ◽

Activity Relationship ◽

Resonance Spectroscopy ◽

Antifungal Activities ◽

Structure Activity ◽

Diethyl Ammonium

β-Pinene can be used as a cheap source to synthesize a large number of high value-added derivatives. In this study, a series of β-pinene derivatives was prepared, and the antifungal activities of the compounds were assessed against phytopathogenic fungi. Eight N-alkyl hydronopyl diethyl ammonium halide salts were synthesized by the reaction of hydronopyl diethyl ammonium halide with 8 halogenated alkanes. The structures of the synthesized products were characterized by Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy and mass spectrometry. The antifungal activities of these derivatives were tested against 11 plant pathogens, and the preliminary structure-activity relationship is discussed. Some derivatives exhibited moderate to significant antifungal activity due to the fusion of the hydronopyl, a long-chain alkyl, bromine, and iodine anionic groups. In contrast to the structure-activity relationship of compounds 2a, 2b, and 2c, iodine ions in 2f, 2g, and 2f had a significant effect on enhancing the antifungal activity against Colletotrichum gloeosporioides, S clerotinia sclerotiorum, Phytophthora capsici, Phomopsis, Sphaeropsis sapinea, Glomerella cingulata, and Fusicoccum aesculi. A higher molecular weight could increase the antifungal activity against Fusarium proliferatum, Alternaria kikuchiana, Sclerotinia sclerotiorum, P. capsici, Phomopsis, and S. sapinea. Compounds 2d and 2e exhibited broad-spectrum antifungal activity against the tested strains. These derivatives are expected to be used as precursor molecules for novel pesticide development in further research.

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ChemInform Abstract: Phthalide Derivatives with Antifungal Activities Against the Plant Pathogens Isolated from the Liquid Culture of Pestalotiopsis photiniae.

ChemInform ◽

10.1002/chin.201214203 ◽

2012 ◽

Vol 43 (14) ◽

pp. no-no

Author(s):

Xiao-Long Yang ◽

Su Zhang ◽

Qiong-Bo Hu ◽

Du-Qiang Luo ◽

Yan Zhang

Keyword(s):

Liquid Culture ◽

Plant Pathogens ◽

Antifungal Activities

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Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to Antifungal Activity and Biofilm Formation

Applied and Environmental Microbiology ◽

10.1128/aem.02645-12 ◽

2012 ◽

Vol 79 (3) ◽

pp. 808-815 ◽

Cited By ~ 96

Author(s):

Zhihui Xu ◽

Jiahui Shao ◽

Bing Li ◽

Xin Yan ◽

Qirong Shen ◽

...

Keyword(s):

Biofilm Formation ◽

Bacillus Amyloliquefaciens ◽

Root Colonization ◽

Antagonistic Activity ◽

Vital Role ◽

Content Type ◽

Reverse Transcription Pcr ◽

Bacillomycin D

ABSTRACTBacillus amyloliquefaciensstrains are capable of suppressing soilborne pathogens through the secretion of an array of lipopeptides and root colonization, and biofilm formation ability is considered a prerequisite for efficient root colonization. In this study, we report that one of the lipopeptide compounds (bacillomycin D) produced by the rhizosphere strainBacillus amyloliquefaciensSQR9 not only plays a vital role in the antagonistic activity againstFusarium oxysporumbut also affects the expression of the genes involved in biofilm formation. When the bacillomycin D and fengycin synthesis pathways were individually disrupted, mutant SQR9M1, which was deficient in the production of bacillomycin D, only showed minor antagonistic activity againstF. oxysporum, but another mutant, SQR9M2, which was deficient in production of fengycin, showed antagonistic activity equivalent to that of the wild-type strain ofB. amyloliquefaciensSQR9. The results fromin vitro, rootin situ, and quantitative reverse transcription-PCR studies demonstrated that bacillomycin D contributes to the establishment of biofilms. Interestingly, the addition of bacillomycin D could significantly increase the expression levels ofkinCgene, but KinC activation is not triggered by leaking of potassium. These findings suggest that bacillomycin D contributes not only to biocontrol activity but also to biofilm formation in strainB. amyloliquefaciensSQR9.

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