HSA Coated Iron Oxide Nanoparticles as Drug Delivery Vehicles for Cancer Therapy

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

Download Full-text

A comprehensive study on 2D, 3D and solid tumor environment to explore a multifunctional biogenic nanoconjugate

Scientific Reports ◽

10.1038/s41598-021-87364-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

B. S. Unnikrishnan ◽

G. U. Preethi ◽

T. T. Sreelekha

Keyword(s):

Drug Delivery ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Tumor Burden ◽

Oxide Nanoparticles ◽

Cell Internalization ◽

Tumor Environment ◽

D Cell ◽

Cancer Spheroids ◽

Comprehensive Study

AbstractEmergence of nanotechnology created a drastic change in the field of cancer therapy due to their unique features in drug delivery and imaging. Polysaccharide based nanoparticles have received extensive attention in recent years as promising nanoparticle mediated drug delivery systems. Polysaccharides are endorsed with versatile merits including high drug encapsulation efficiency, efficient drug protection against chemical or enzymatic degradation, unique ability to create a controlled release and cellular internalization. In the current study, we have fabricated doxorubicin-loaded carboxymethylated PST001 coated iron oxide nanoparticles (DOX@CM-PST-IONPs) for better management of cancer. CM-PST coated iron oxide nanoparticles co-encapsulated with chemotherapeutic drug doxorubicin, can be utilized for targeted drug delivery. Biocompatible and non-toxic nanoconjugates was found to be effective in both 2-D and 3-D cell culture system with efficient cancer cell internalization. The bench-marked potential of CM-PIONPs to produce reactive oxygen species makes it a noticeable drug delivery system to compact neoplasia. These nanoconjugates can lay concrete on a better way for the elimination of cancer spheroids and tumor burden.

Download Full-text

Dendrimers and Dendrimers-Grafted Superparamagnetic Iron Oxide Nanoparticles: Synthesis, Characterization, Functionalization, and Biological Applications in Drug Delivery Systems

Nano- and Microscale Drug Delivery Systems ◽

10.1016/b978-0-323-52727-9.00005-4 ◽

2017 ◽

pp. 75-94

Author(s):

Asghar Taheri-Kafrani ◽

Hamidreza Shirzadfar ◽

Elham Tavassoli-Kafrani

Keyword(s):

Drug Delivery ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

Drug Delivery Systems ◽

Superparamagnetic Iron Oxide ◽

Delivery Systems ◽

Superparamagnetic Iron Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Biological Applications ◽

Nanoparticles Synthesis

Download Full-text

Using Chitosan or Chitosan Derivatives in Cancer Therapy

Polysaccharides ◽

10.3390/polysaccharides2040048 ◽

2021 ◽

Vol 2 (4) ◽

pp. 795-816

Author(s):

Md Salman Shakil ◽

Kazi Mustafa Mahmud ◽

Mohammad Sayem ◽

Mahruba Sultana Niloy ◽

Sajal Kumar Halder ◽

...

Keyword(s):

Drug Delivery ◽

Cancer Therapy ◽

Anticancer Agents ◽

Chitosan Nanoparticles ◽

Chemotherapeutic Drugs ◽

Chitosan Derivatives ◽

Drug Delivery Vehicles ◽

Modified Chitosan ◽

Therapeutic Benefits ◽

Delivery Vehicles

Cancer is one of the major causes of death worldwide. Chemotherapeutic drugs have become a popular choice as anticancer agents. Despite the therapeutic benefits of chemotherapeutic drugs, patients often experience side effects and drug resistance. Biopolymers could be used to overcome some of the limitations of chemotherapeutic drugs, as well as be used either as anticancer agents or drug delivery vehicles. Chitosan is a biocompatible polymer derived from chitin. Chitosan, chitosan derivatives, or chitosan nanoparticles have shown their promise as an anticancer agent. Additionally, functionally modified chitosan can be used to deliver nucleic acids, chemotherapeutic drugs, and anticancer agents. More importantly, chitosan-based drug delivery systems improved the efficacy, potency, cytotoxicity, or biocompatibility of these anticancer agents. In this review, we will investigate the properties of chitosan and chemically tuned chitosan derivatives, and their application in cancer therapy.

Download Full-text