Microbiological analysis of root canals associated with periapical abscesses (with pain and swelling) and the antimicrobial susceptibility of isolated bacteria

2006 ◽  
Vol 18 (4) ◽  
pp. 327-337 ◽  
Author(s):  
Selcuk M. Ozbek ◽  
M. Sinan Evcil ◽  
Ahmet Ozbek ◽  
Aziz Sahin Erdoğan ◽  
Mustafa Köseoğlu
Keyword(s):  
Antimicrobial Susceptibility ◽  
Microbiological Analysis ◽  
Root Canals

scholarly journals The effect of timing temporary cements to treat induced pulp necrosis in the teeth of dogs

2007 ◽  
Vol 27 (2) ◽  
pp. 85-88
Author(s):  
Léslie M. Domingues-Falqueiro ◽  
Jonathan Ferreira ◽  
Fernanda M. Lopes ◽  
Agnes Tymoszczenko ◽  
Marco A. Gioso
Keyword(s):  
Root Canal ◽  
Microbial Reduction ◽  
Endodontic Treatment ◽  
Microbiological Analysis ◽  
Single Session ◽  
Root Canal Filling ◽  
Root Canals ◽  
Gutta Percha ◽  
Dressing Materials ◽  
Block Section

During endodontic therapy (pulpectomy, root canal debridement and root canal filling) microbiological management is a major concern. Bacteria present in dentine tubules, apical foramina and apical delta are causally related to failure of the procedure. Studies have shown that during single session endodontic treatment bacteria remain within dental structures. The aim of the present study was to evaluate endodontic treatment performed as two sessions, using temporary endodontic dressing materials for different periods in four groups of experimental dogs. A total of 80 roots of second and third upper premolar teeth and second, third and fourth lower premolar teeth were divided into four groups. The pulp chamber was opened with burrs and the pulp exposed for 60 days to induce pulpal inflammation and necrosis. Groups II, III and IV were treated with calcium hydroxide plus camphorated paramono-chlorophenol (PMCC) for 7, 15 and 30 days, respectively. In all groups, the root canals were filled with zinc oxide-eugenol and gutta-percha cones. Clinical and radiographical measurements were performed every 2 weeks. After 60 days a small block section containing the teeth, surrounding periapical tissues and the periodontium was removed for histological and microbiological study. Histological analysis revealed intense inflammatory response in all groups. Microbiological analysis showed microbial reduction inversely proportional to the period of time that the intracanal temporary medicament was left in place.

scholarly journals Bacterial etiology of Keratitis and their antimicrobial susceptibility pattern at a tertiary care hospital.

2020 ◽  
Vol 35 (4) ◽  
pp. 271-275
Author(s):  
Fahd Kamal Akhtar ◽  
Zubair Saleem ◽  
Muna Malik ◽  
Adeel Chaudhry ◽  
Ayesha Sajjad ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Microbiological Analysis ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Sample Collection ◽  
Agar Culture ◽  
Wire Loop ◽  
Antimicrobial Susceptibility Pattern

Purpose: The aim of this study was to find out Bacterial etiology and antimicrobial susceptibility pattern of bacteria causing infective keratitis at a tertiary care hospital of LahoreStudy Design:Descriptive cross sectional studyStudy Place and Study Period: PGMI/AMC/LGH, Lahore, in 2015 - 2018. Sample Collection: Non-probability convenience samplingMaterial and Methods: The corneal scrapings taken by the ophthalmologist was immediately inoculated on Chocolate agar, Blood agar and MacConkay agar culture plates with the help of sterilized wire loop in the operation theater.These plates were taken to laboratory for incubation and further microbiological processing. After inoculating on the culture agar plates, the scrapings from cornea were smeared on glass slides with the help of sterilized wire loop. The smears were air-dried stained with Gram’s stain, Kinyoun stain and Giemsa stain. The culture and staining were further processed for microbiological analysis of bacterial etiology and antimicrobial susceptibility.Results:7 of each cases of Staphylococcus aureus and Streptococcus pneumoniae were isolated accounting 28% of total bacteria. Gram negative bacteria are relatively sensitive to all groups of antibiotics as compared to Gram positive bacteria who were resistant of major groups of antibiotic like Fluoroquinolones and aminoglycosides. Conclusion:We concluded that a cumulative antimicrobial data should be published from laboratory on antimicrobial susceptibiblity pattern of bacterial keratitis for its empirical treatment.

scholarly journals Microbiological Analysis of Primary Infected Root Canals with Symptomatic and Asymptomatic Apical Periodontitis of Young Permanent Teeth

2020 ◽  
Vol 24 (3) ◽  
pp. 44-51
Author(s):  
Jelena Marinković ◽  
Tatjana Marković ◽  
Snežana Brkić ◽  
Milena Radunović ◽  
Ivan Soldatović ◽  
...  
Keyword(s):  
School Children ◽  
Clinical Symptoms ◽  
Treatment Strategies ◽  
Apical Periodontitis ◽  
Permanent Teeth ◽  
Microbiological Analysis ◽  
Microbial Composition ◽  
Root Canals ◽  
Facultative Anaerobes ◽  
Infected Root

SummaryBackground/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms.Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symtoms were recorded.Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms.Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.

scholarly journals Bacteria profile and antibiogram of the bacteria isolated from the exposed pulp of dog canine teeth

2018 ◽  
Vol 183 (3) ◽  
pp. 97-97 ◽  
Author(s):  
José Carlos Almansa Ruiz ◽  
Annelize Jonker ◽  
Anna-Mari Bosman ◽  
Gerhard Steenkamp
Keyword(s):  
Antimicrobial Susceptibility ◽  
Anaerobic Bacteria ◽  
Diffusion Test ◽  
Gram Positive ◽  
Gram Negative ◽  
Root Canals ◽  
Microbial Species ◽  
Facultative Anaerobes

Twenty-seven microbiological samples were taken from root canals (RC) of the canine teeth of 20 dogs where the pulps were non-vital and exposed due to complicated crown fractures. These pulps were cultured for aerobic/anaerobic bacteria. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer diffusion test. A total of 49 cultivable isolates, belonging to 27 different microbial species and 18 different genera, were recovered from the 27 RCs sampled. Twenty (40.81 per cent) of the cultivable isolates were Gram positive while 29 (59.19 per cent) were Gram negative. Facultative anaerobes were the most common bacteria (77.56 per cent). Aerobic isolates represented 18.36 per cent, and strict anaerobes 4.08 per cent. The antimicrobials with the highest in vitro efficacy were gentamicin (100 per cent) and enrofloxacin (93.32 per cent).

scholarly journals Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing

2019 ◽  
Vol 116 (21) ◽  
pp. 10270-10279 ◽  
Author(s):  
Hui Li ◽  
Peter Torab ◽  
Kathleen E. Mach ◽  
Christine Surrette ◽  
Matthew R. England ◽  
...  
Keyword(s):  
Single Cell ◽  
Antimicrobial Susceptibility ◽  
Bacterial Infections ◽  
Susceptibility Testing ◽  
Bacterial Pathogens ◽  
Microfluidic System ◽  
Antimicrobial Susceptibility Testing ◽  
Microbiological Analysis ◽  
Single Cell Level ◽  
Cell Level

Infectious diseases caused by bacterial pathogens remain one of the most common causes of morbidity and mortality worldwide. Rapid microbiological analysis is required for prompt treatment of bacterial infections and to facilitate antibiotic stewardship. This study reports an adaptable microfluidic system for rapid pathogen classification and antimicrobial susceptibility testing (AST) at the single-cell level. By incorporating tunable microfluidic valves along with real-time optical detection, bacteria can be trapped and classified according to their physical shape and size for pathogen classification. By monitoring their growth in the presence of antibiotics at the single-cell level, antimicrobial susceptibility of the bacteria can be determined in as little as 30 minutes compared with days required for standard procedures. The microfluidic system is able to detect bacterial pathogens in urine, blood cultures, and whole blood and can analyze polymicrobial samples. We pilot a study of 25 clinical urine samples to demonstrate the clinical applicability of the microfluidic system. The platform demonstrated a sensitivity of 100% and specificity of 83.33% for pathogen classification and achieved 100% concordance for AST.

Comparative Evaluation of Bactericidal Potential of Four Root Canal Filling Materials against Microflora of Infected Non -Vital Primary Teeth

2010 ◽  
Vol 35 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Priya Harini ◽  
Sham Bhat ◽  
K Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Primary Teeth ◽  
Microbiological Analysis ◽  
Filling Material ◽  
Root Canal Filling ◽  
Posterior Teeth ◽  
Root Canals ◽  
Filling Materials ◽  
Root Canal Filling Materials

Background and objectives: Since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use of antibacterial irrigating agents and root canal filling materials. Recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals of primary teeth. The study determined the antibacterial effectiveness of four root canal filling materials namely Calcium hydroxide,Zinc oxide eugenol, Vitapex and Metapex against microbial specimens obtained directly from necrotic root canals of primary teeth. Method: Microbial specimens were collected using sterile paper points, from 15 primary maxillary and mandibular posterior teeth of randomly selected children in the age group of 4-10 years with infected non vital primary teeth, requiring pulpectomy procedure. The microbial specimens collected were subjected to microbiological analysis and the antimicrobial potential of root canal filling materials were tested using Agar diffusion technique. Results: were statistically analyzed using one-way ANOVA. Facultative/Aerobic organisms were isolated in all the cases, anaerobic organisms were isolated in 80% of the cases, and Candida albicans was isolated in 1 case. ZOE showed superior inhibitory activity against most of the organisms isolated followed by Vitapex, Calcium hydroxide and Metapex in descending order.Conclusion: Our data may be useful as a guide for relative antimicrobial effectiveness or non-effectiveness of the materials employed. In vivo studies are required to state the specific antimicrobial activity and merits and demerits of any of the test filling material.

scholarly journals Bacterial isolates and their antimicrobial susceptibility pattern among patients with ocular infections at the University of Gondar Hospital, Northwest Ethiopia: A 10-years retrospective study

2019 ◽  
Author(s):  
Daniel Seifu ◽  
Amanuel Tesfaye ◽  
Zufan Yiheyis ◽  
Mekin Mohammed ◽  
Gizeaddis Belay ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bacterial Species ◽  
Northwest Ethiopia ◽  
University Hospital ◽  
Multi Drug Resistance ◽  
Microbiological Analysis ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Gram Positive ◽  
Antimicrobial Susceptibility Pattern

Abstract Abstract Objective: The aim of this study was to identify potential bacterial isolates and their antimicrobial susceptibility pattern among patients who visited eye clinic of Gondar university hospital. Results: From year of January 2009 to January 2019 a total of 319 eye discharge specimens were submitted for microbiological analysis, of which 133(41.7%) were culture positive. A total of 42(31.6%) Gram negatives and 91(68.4%) Gram-positive bacteria were isolated. Among isolated Gram-negative bacterial species, Klebsiella spp was the most predominant, 42.9% followed by E. coli 26.2%. Among isolated Gram-positive pathogenic bacterial species, S. aureus was the most prevalent, 59.5% followed by S. pyogenes , 8% and S. pneumoniae , 5.5%. Most of the bacterial isolates were resistant to cotrimoxazole, 81.6%, amoxicillin, 78.7%, tetracycline,76.2%, and ampicillin 75.9%. About 47.4 % of bacterial isolates showed multi drug resistance to three or more classes of antimicrobials. Antimicrobials like: ampicillin, amoxicillin, ceftriaxone, ciprofloxacin, and norfloxacin exhibited year to year increment of resistance (p<0.0001).

scholarly journals Clinico-microbiological analysis of bactibilia isolates in patients of cholecystectomy

2017 ◽  
Vol 5 (9) ◽  
pp. 4030
Author(s):  
Ashima Katyal ◽  
Kiran Bala ◽  
Anand Bansal ◽  
Uma Chaudhary
Keyword(s):  
Gall Bladder ◽  
Antimicrobial Susceptibility ◽  
Histopathological Examination ◽  
Resistance Mechanism ◽  
Microbiological Analysis ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Group A ◽  
Resistant Strains ◽  
Group B

Background: The current study determined microbial flora in bile aspirates from patients undergoing cholecystectomy along with antibiotic susceptibility pattern and resistance mechanism viz. extended spectrum β-lactamase (ESBL).Methods: The study included 110 such patients which were divided in three groups as: Group A that included acute cholecystitis with or without cholelithiasis, Group B included chronic cholecystitis with or without cholelithiasis and Group C included other hepatobiliary ailments requiring cholecystectomy viz. gallbladder carcinoma, acute emphysematous gall bladder and mucocele gall bladder. The bile was subjected to complete microbiological and histopathological examination. Antimicrobial susceptibility of the isolates was performed as per CLSI guidelines.Results: Bacteria were recovered from 07 samples (20%) in Group A, 17 (48.57%) in Group B and 11 (31.43%) in Group C. The polymicrobial infection was seen in 04 (11.43%) patients. The most common organisms isolated were Escherichia coli 15 (38.46%), Klebsiella pneumoniae and Pseudomonas aeruginosa 8 (20.51% each). The majority of Enterobacteriaceae isolates were susceptible to piperacillin-tazobactam and carbapenems. The ESBL production was observed in 17 (43.58%) isolates.Conclusions: Therefore, antimicrobial susceptibility of potential causative organisms, presence of resistant strains in bile, the severity of the cholecystitis, and the local susceptibility pattern must be taken into consideration while prescribing antibiotics. A protocol regarding the management of such cases should be formulated based on observations of similar studies. 

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