Steady-state chlorophyll fluorescence (Fs) measurements as a tool to follow variations of net CO2 assimilation and stomatal conductance during water-stress in C3 plants

2002 ◽  
Vol 114 (2) ◽  
pp. 231-240 ◽  
Author(s):  
Jaume Flexas ◽  
José Mariano Escalona ◽  
Sebastian Evain ◽  
Javier Gulías ◽  
Ismaël Moya ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Steady State ◽  
Water Stress ◽  
Stomatal Conductance ◽  
Co2 Assimilation ◽  
C3 Plants ◽  
Net Co2 Assimilation

Steady-State and Maximum Chlorophyll Fluorescence Responses to Water Stress in Grapevine Leaves

2000 ◽  
Vol 73 (3) ◽  
pp. 283-297 ◽  
Author(s):  
Jaume Flexas ◽  
Jean-Marie Briantais ◽  
Zoran Cerovic ◽  
Hipólito Medrano ◽  
Ismael Moya
Keyword(s):  
Chlorophyll Fluorescence ◽  
Steady State ◽  
Water Stress ◽  
Grapevine Leaves

scholarly journals Influence of Surflan (Oryzalin) Concentrations in Irrigation Water on Growth and Physiological Processes of Gardenia jasminoides radicans and Pennisetum rupelli

1997 ◽  
Vol 15 (4) ◽  
pp. 169-172
Author(s):  
Rajgopal Bhandary ◽  
Ted Whitwell ◽  
Jeanne Briggs ◽  
R. Thomas Fernandez
Keyword(s):  
Stomatal Conductance ◽  
Irrigation Water ◽  
Treatment Initiation ◽  
Co2 Assimilation ◽  
Ethylene Synthesis ◽  
Sensitive Species ◽  
Ethylene Evolution ◽  
Gardenia Jasminoides ◽  
Physiological Processes ◽  
Net Co2 Assimilation

Abstract This study investigated the effect of oryzalin concentrations on growth, CO2 assimilation, stomatal conductance, transpiration and ethylene synthesis in dwarf gardenia and fountain grass (tolerant and sensitive species). The plant species were subjected to irrigation water containing 10 μg/liter, 100 μg/liter and 1000 μg/liter oryzalin. CO2 assimilation, stomatal conductance and transpiration were measured 2 and 4 weeks after treatment initiation, and ethylene evolution was determined at the end of the study. Oryzalin did not affect net CO2 assimilation, stomatal conductance, transpiration or ethylene evolution for dwarf gardenia or fountain grass. Dwarf gardenia shoot and root weights were not affected by oryzalin, but shoot and root weights of fountain grass were reduced by the highest concentration.

Water transport from stem to stomata: the coordination of hydraulic and gas exchange traits across 33 subtropical woody species

2019 ◽  
Vol 39 (10) ◽  
pp. 1665-1674 ◽  
Author(s):  
Xiaorong Liu ◽  
Hui Liu ◽  
Sean M Gleason ◽  
Guillermo Goldstein ◽  
Shidan Zhu ◽  
...  
Keyword(s):  
Stomatal Conductance ◽  
Gas Exchange ◽  
Water Transport ◽  
Explanatory Power ◽  
Woody Species ◽  
Hydraulic Conductance ◽  
Co2 Assimilation ◽  
Leaf Hydraulic Conductance ◽  
Net Co2 Assimilation ◽  
Leaf Tissues

Abstract Coordination between sapwood-specific hydraulic conductivity (Ks) and stomatal conductance (gs) has been identified in previous studies; however, coordination between leaf hydraulic conductance (Kleaf) and gs, as well as between Kleaf and Ks is not always consistent. This suggests that there is a need to improve our understanding of the coordination among hydraulic and gas exchange traits. In this study, hydraulic traits (e.g., Ks and Kleaf) and gas exchange traits, including gs, transpiration (E) and net CO2 assimilation (Aarea), were measured across 33 co-occurring subtropical woody species. Kleaf was divided into two components: leaf hydraulic conductance inside the xylem (Kleaf-x) and outside the xylem (Kleaf-ox). We found that both Kleaf-x and Kleaf-ox were coordinated with gs and E, but the correlations between Kleaf-ox and gs (or E) were substantially weaker, and that Ks was coordinated with Kleaf-x, but not with Kleaf-ox. In addition, we found that Ks, Kleaf-x and Kleaf-ox together explained 63% of the variation in gs and 42% of the variation in Aarea across species, with Ks contributing the largest proportion of explanatory power, whereas Kleaf-ox contributed the least explanatory power. Our results demonstrate that the coordination between leaf water transport and gas exchange, as well as the hydraulic linkage between leaf and stem, were weakened by Kleaf-ox. This highlights the possibility that water transport efficiencies of stem and leaf xylem, rather than that of leaf tissues outside the xylem, are important determinants of stomatal conductance and photosynthetic capacity across species.

Water Stress Effects on Variations of Steady-State Chlorophyll Fluorescence (Fs) in Response to Light

1998 ◽  
pp. 2589-2593
Author(s):  
Jaume Flexas ◽  
José M. Escalona ◽  
José Cifre ◽  
Hipólito Medrano
Keyword(s):  
Chlorophyll Fluorescence ◽  
Steady State ◽  
Water Stress ◽  
Stress Effects

Carbon Isotope Discrimination measured Concurrently with Gas Exchange to Investigate CO2 Diffusion in Leaves of Higher Plants

1986 ◽  
Vol 13 (2) ◽  
pp. 281 ◽  
Author(s):  
JR Evans ◽  
TD Sharkey ◽  
JA Berry ◽  
GD Farquhar
Keyword(s):  
Stomatal Conductance ◽  
Gas Exchange ◽  
Carbon Isotope ◽  
Higher Plants ◽  
Isotope Composition ◽  
Co2 Assimilation ◽  
Carbon Isotope Composition ◽  
C3 Plants ◽  
Isotopic Discrimination ◽  
Co2 Diffusion

Conventional gas-exchange techniques that measure the stomatal conductance and rate of CO2 assimilation of leaves were combined with measurements of the carbon isotope composition of CO2 in air passing over a leaf. Isotopic discrimination during uptake was determined from the difference in the carbon isotope composition of air entering and leaving the leaf chamber. Isotopic discrimination measured over the short term correlated strongly with that determined from combusted leaf material. Environmental conditions were manipulated to alter the relative influences of stomatal conductance and carboxylation on the discrimination of carbon isotopes by intact leaves. With C3 plants, discrimination increased as the gradient in partial pressure of CO2 across the stomata decreased. For C4 plants there was little change in discrimination despite substantial changes in the diffusion gradient across the sto- mata. These results are consistent with, and provide the first direct experimental support for, theoretical equations describing discrimination during photosynthesis. Despite uncertainties about various processes affecting carbon isotope composition, the resistance to the transfer of CO2 from the intercellular airspaces to the sites of carboxylation in the mesophyll chloroplasts was estimated using this technique. For wheat the estimated resistance was 1.2-2.4 m2 s bar mol -1.

Photoprotection in water-stressed plants of durum wheat (Triticum turgidum var. durum): changes in chlorophyll fluorescence, spectral signature and photosynthetic pigments

2002 ◽  
Vol 29 (1) ◽  
pp. 35 ◽  
Author(s):  
Eduardo A. Tambussi ◽  
Jaume Casadesus ◽  
Sergi Munné-Bosch ◽  
José Luis Araus
Keyword(s):  
Chlorophyll Fluorescence ◽  
Water Stress ◽  
Durum Wheat ◽  
Triticum Turgidum ◽  
Plant Stress ◽  
Co2 Assimilation ◽  
Photochemical Quenching ◽  
Spectral Signature ◽  
Antioxidant Defences ◽  
Carotene Content

We analysed the photoprotective response in water-stressed plants of durum wheat (Triticum turgidum L. var. durum cv. Mexa). The plants were grown in a greenhouse for 4 weeks and then exposed to water stress by withholding water for 8 d. Development of water stress was monitored as the decrease in relative water content (RWC) and net CO2 assimilation of the last fully developed leaf. The photoprotective response was evaluated in the same leaves by analysing modulated chlorophyll fluorescence, leaf spectroradiometrical changes, and pigment content. Measurements were performed 3, 6 (moderate stress) and 8 (severe stress) d after water-stress treatment began. The non-photochemical quenching of chlorophyll fluorescence (qN), as well as the contents of zeaxanthin and antheraxanthin increased significantly after 6 d of treatment. However, a further rise in these xanthophylls on day 8 was not associated with any increase in qN. In addition, the β-carotene content rose significantly on day 8, suggesting an increase in antioxidant defences. The photochemical index (PI), derived from spectroradiometrical measurements, showed a strong progressive drop on days 6 and 8, which was paralleled by an increase in the de-epoxidation state of the xanthophyll cycle (DPS), in particular by the zeaxanthin content. At midday, PI was strongly (negatively) correlated with DPS and qN. These results suggest that the PI may be a reliable indicator of photoprotection in the study of plant stress, and in breeding programs.

scholarly journals Effect of drought on gas exchange and chlorophyll fluorescence of groundnut genotypes

2021 ◽  
Vol 13 (4) ◽  
pp. 1478-1487
Author(s):  
K. Manojkumar ◽  
S. Vincent ◽  
M. Raveendran ◽  
R. Anandham ◽  
V. Babu Rajendra Prasad ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Water Stress ◽  
Stomatal Conductance ◽  
Gas Exchange ◽  
Photosynthetic Rate ◽  
Photosynthetic Activity ◽  
Water Deficit Stress ◽  
Abiotic Factor ◽  
Gas Exchange Parameters ◽  
Ps Ii

Drought is one of the major threats to groundnut productivity, causing a greater loss than any other abiotic factor. Water stress conditions alter plant photosynthetic activity, impacting future growth and assimilating mobilization towards sink tissues. The purpose of this study was to investigate how drought impacts the photosynthesis of plants and its links to drought tolerance. The influence of reproductive stage drought on photosynthetic activity and chlorophyll fluorescence of groundnut is well studied. The experiment was conducted in Kharif 2019 (Jul-Sep), where recent series in groundnut genotypes (60 nos) sown under rainfed conditions and water stress was created by withholding irrigation for 20 days between 35-55 days after sowing in the field to simulate drought conditions. Imposition of water deficit stress reduced PS II efficiency, which significantly altered the photosynthetic rate in the leaf. Observation of gas exchange parameters viz., photosynthetic rate, stomatal conductance and transpiration rate after 20 days of stress imposition revealed that of all 60 genotypes, 20 genotypes (VG 17008, VG 17046VG 18005, VG 18102, VG 18077, VG 19572, VG 19709, VG 18111, VG19561, VG19576, VG 19620, VG 19681, VG 19688, etc.,) had better Photosynthetic rate, Stomatal conductance. Similarly, PS II efficiency analyzed through fluorescence meter revealed that among the 60 and all the genotypes given above recorded higher value in Fv/Fm. Results obtained from Cluster analysis and PCA confirmed that photosynthetic rate and Fv/Fm is useful parameter in screening adapted cultivars under drought stress. These findings lay the groundwork for a future study to decipher the molecular pathways underpinning groundnut drought resistance.

scholarly journals MATTER ACCUMULATION AND PHOTOSYNTHETIC RESPONSES OF MACAW PALM TO CYCLICAL DROUGHT

2016 ◽  
Vol 29 (4) ◽  
pp. 850-858 ◽  
Author(s):  
CLENILSO SEHNEN MOTA ◽  
MARCO ANTONIO OLIVA CANO
Keyword(s):  
Water Stress ◽  
Co2 Assimilation ◽  
Leaf Water ◽  
Photosynthetic Parameters ◽  
Dry Matter Accumulation ◽  
Stomatal Limitation ◽  
Net Co2 Assimilation ◽  
Significant Difference ◽  
Macaw Palm ◽  
Photosynthetic Responses

ABSTRACT The objective of this work was to evaluate the plant-water relations, photosynthetic parameters and growth of macaw (Acrocomia aculeata (Jacp.) Lodd. ex Mart.) plants, thus, ten plants were subjected to three successive drought and rehydration cycles and ten other plants were irrigated normally as control. The drought cycles consisted of a suspension of irrigation until the net CO2 assimilation rate (A) reach values lower than 5% of the control, and a rehydration until a plants recover at least 85% of A of the control plants. Reductions in A, stomatal conductance (gs ) and transpiration (E), above 95%, were found with predawn leaf water potential (Ψpd) of -1.85 MPa. After rehydration, the gs and E of plants with and without water stress was not different, however, A presented a significant difference. Leaf water potentials below -0.5 MPa reduced the intracellular and atmospheric CO2 ratio and increased water use efficiency, and values of -1.85 MPa, when both showed an increase and decrease, respectively. The reduction of A and gs was linear and proportional to Ψpd. Total dry matter accumulation reduced by 50% in the plants subjected to drought. The cyclic water stress resulted in reduction of gas exchange and matter accumulation in macaw palm plants; a stomatal limitation of A occurred until Ψpd of -1.85 MPa, and then a non-stomatal limitation.

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