scholarly journals Pharmacokinetic and metabolomic analyses of the neuroprotective effects of salvianolic acid A in a rat ischemic stroke model

2017 ◽  
Vol 38 (11) ◽  
pp. 1435-1444 ◽  
Author(s):  
Si-qi Feng ◽  
Nan Aa ◽  
Jian-liang Geng ◽  
Jing-qiu Huang ◽  
Run-bin Sun ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Stroke Model ◽  
Neuroprotective Effects ◽  
Salvianolic Acid ◽  
Salvianolic Acid A

Gelatin nanoparticles enhance the neuroprotective effects of intranasally administered osteopontin in rat ischemic stroke model

2014 ◽  
Vol 4 (5-6) ◽  
pp. 395-399 ◽  
Author(s):  
Elizabeth Joachim ◽  
Il-Doo Kim ◽  
Yinchuan Jin ◽  
Kyekyoon Kim ◽  
Ja-Kyeong Lee ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Stroke Model ◽  
Gelatin Nanoparticles ◽  
Neuroprotective Effects

Cerebral Protection of Salvianolic Acid A by the Inhibition of Granulocyte Adherence

2011 ◽  
Vol 39 (01) ◽  
pp. 111-120 ◽  
Author(s):  
Min Jiang ◽  
Xiao-Ying Wang ◽  
Wang-Yi Zhou ◽  
Jing Li ◽  
Jie Wang ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Vascular Endothelial Cells ◽  
Leukocyte Adhesion ◽  
Ischemia Reperfusion ◽  
Experimental Studies ◽  
Cerebral Protection ◽  
Active Components ◽  
Salvianolic Acid ◽  
Salvianolic Acid A ◽  
Adhesion Rate

Ischemic stroke is one of the most common causes of death and disability that is induced by ischemia reperfusion (IR). Granulocyte adherence has been proven to be a principal cause of IR. Salvianolic acid A (Sal A) is one of the major active components of Danshen, a Chinese herbal medicine used for the treatment of cardiovascular and cerebrovascular diseases, such as ischemic stroke. Some experimental studies have shown the strong cerebral protection effect of Sal A. However, little information is available about the effect of Sal A on granulocyte adherence to brain micro-vascular endothelial cells (BMEC). Therefore, the aim of the present study was to investigate the effect of Sal A on the leukocyte adhesion rate and the intercellular cell adhesion molecule-1 (ICAM-1) expression in BMEC injured by hypoxia/reoxygenation (H/R), using a rheometer, qRT-PCR, and flow cytometry (FCM). The results of the adhesion rate gathered by the rheometer showed that Sal A could remarkably inhibit the adherence of granulocytes on BMEC in the case of H/R injury. Moreover, PCR and FCM results showed that Sal A could decrease the expression of ICAM-1 on BMEC on the gene and protein levels. In conclusion, the study demonstrated that the inhibition of granulocyte adherence is one of the targets of Sal A in the treatment of ischemic stroke. Meanwhile, Sal A inhibits of granulocyte adherence by decreasing the expression of ICAM-1 in BMEC.

scholarly journals Stroke neuroprotection revisited: Intra-arterial verapamil is profoundly neuroprotective in experimental acute ischemic stroke

2015 ◽  
Vol 36 (4) ◽  
pp. 721-730 ◽  
Author(s):  
Michael E Maniskas ◽  
Jill M Roberts ◽  
Ishi Aron ◽  
Justin F Fraser ◽  
Gregory J Bix
Keyword(s):  
Ischemic Stroke ◽  
Side Effects ◽  
Channel Blocker ◽  
Infarct Volume ◽  
Stroke Model ◽  
Neuroprotective Effects ◽  
Injection Volume ◽  
Cellular Apoptosis ◽  
Systemic Side Effects

While clinical trials have now solidified the role of thrombectomy in emergent large vessel occlusive stroke, additional therapies are needed to optimize patient outcome. Using our previously described experimental ischemic stroke model for evaluating adjunctive intra-arterial drug therapy after vessel recanalization, we studied the potential neuroprotective effects of verapamil. A calcium channel blocker, verapamil is often infused intra-arterially by neurointerventionalists to treat cerebral vasospasm. Such a direct route of administration allows for both focused targeting of stroke-impacted brain tissue and minimizes potential systemic side effects. Intra-arterial administration of verapamil at a flow rate of 2.5 µl/min and injection volume of 10 µl immediately after middle cerebral artery recanalization in C57/Bl6 mice was shown to be profoundly neuroprotective as compared to intra-arterial vehicle-treated stroke controls. Specifically, we noted a significant (P ≤ 0.05) decrease in infarct volume, astrogliosis, and cellular apoptosis as well as a significant increase in neuronal survival and functional outcome over seven days. Furthermore, intra-arterial administration of verapamil was well tolerated with no hemorrhage, systemic side effects, or increased mortality. Thus, verapamil administered intra-arterially immediately following recanalization in experimental ischemic stroke is both safe and neuroprotective and merits further study as a potential therapeutic adjunct to thrombectomy.

scholarly journals Dietary Supplementation Exerts Neuroprotective Effects in Ischemic Stroke Model

2008 ◽  
Vol 11 (1) ◽  
pp. 201-214 ◽  
Author(s):  
Takao Yasuhara ◽  
Koichi Hara ◽  
Mina Maki ◽  
Tadashi Masuda ◽  
Cyndy D. Sanberg ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Dietary Supplementation ◽  
Stroke Model ◽  
Neuroprotective Effects

Heat Shock Protein 70 kDa as a Target for Diagnostics and Therapy of Cardiovascular and Cerebrovascular Diseases

2019 ◽  
Vol 25 (6) ◽  
pp. 710-714 ◽  
Author(s):  
Ekaterina V. Konstantinova ◽  
Natalia S. Chipigina ◽  
Marina H. Shurdumova ◽  
E.I. Kovalenko ◽  
Alexander M. Sapozhnikov
Keyword(s):  
Myocardial Infarction ◽  
Ischemic Stroke ◽  
Heat Shock ◽  
Cerebrovascular Diseases ◽  
Peripheral Blood Lymphocytes ◽  
Neuroprotective Effects ◽  
Adverse Conditions ◽  
Shock Proteins ◽  
Diagnostics And Therapy ◽  
Main Factor

Acute focal ischemia is a main factor of pathogenesis of a number of widespread cardiovascular and cerebrovascular diseases, in particular, myocardial infarction and ischemic stroke. It is known that under the conditions of ischemia expression of intracellular heat shock proteins (HSPs), especially HSP70, grows greatly irrespective of the cell type. This stress-induced cell response is connected with cytoprotective properties of HSP70. The protective functions of HSP70 contribute to the cell survival under adverse conditions and inhibit development of programmed cell death. It was shown, that the level of HSP70 increases in cardiomyocytes and brain cells in response to ischemia, that was connected with cardioprotective and neuroprotective effects. Besides, in recent years, clinical studies of HSP70 have demonstrated elevated level of HSP70 in peripheral blood lymphocytes in groups of patients with ischemic stroke and myocardial infarction. This review indicates that HSP70 can serve as a target for developing new approaches to diagnostics and therapy of cardiovascular and cerebrovascular diseases.

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