scholarly journals Biological efficacy of perpendicular type-I collagen protruded from TiO2-nanotubes

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Chia-Yu Chen ◽  
David. M. Kim ◽  
Cliff Lee ◽  
John Da Silva ◽  
Shigemi Nagai ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Type I Collagen ◽  
Cell Attachment ◽  
In Vitro Study ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Type I ◽  
Nih3t3 Cells ◽  
Biological Efficacy

AbstractThe aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO2 nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO2 nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO2 surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO2 nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal.

scholarly journals Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays

2021 ◽  
Vol 19 ◽  
pp. 228080002198969
Author(s):  
Min-Xia Zhang ◽  
Wan-Yi Zhao ◽  
Qing-Qing Fang ◽  
Xiao-Feng Wang ◽  
Chun-Ye Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Type I Collagen ◽  
Inhibition Zone ◽  
Negative Control ◽  
Type I ◽  
Nih3t3 Cells ◽  
Post Operation

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

Effect of Poly (Lactic–Co–Glycolic Acid) (75:25)/ Hydroxyapatite Composite on Chondrocyte Culture for Tissue Reconstruction

2007 ◽  
Vol 342-343 ◽  
pp. 353-356 ◽  
Author(s):  
Jung Bok Lee ◽  
Seong Mi Yu ◽  
Sang Gil Lee ◽  
Jae Bong Choi ◽  
Jeong Koo Kim
Keyword(s):  
Composite Film ◽  
Cell Attachment ◽  
Composite Films ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Control Group ◽  
Cellular Attachment ◽  
Control Film ◽  
Content Ratio

PLGA (75:25)/hydroxyapatite (HA) composite films were fabricated by solvent-casting method to investigate the effect of various hydroxyapatite content ratio to the PLGA film for cellular attachment and proliferation. Mechanical property of the composite film was characterized by tensile test. The ultimate tensile strength of 10% HA content film was two folds higher than control group. The surface of the film was characterized by contact angle measurement. The PLGA/HA composite film was more hydrophilic than control film. In vitro chondrocyte responses to the composite films were measured by cellular attachment and proliferation test. The attached and proliferated cells were significantly higher on PLGA/HA (10%) composite film than control group (1.44 times higher in attachment test and 1.31 times higher for 6th-day at culture in proliferation assaying, p<0.05). Base on these finding, the PLGA/HA (10%) composite was effective for the cell attachment for the initial stage of cultivation and cell proliferation.

scholarly journals A durable and biocompatible ascorbic acid-based covalent coating method of polydimethylsiloxane for dynamic cell culture

2017 ◽  
Vol 14 (132) ◽  
pp. 20170318 ◽  
Author(s):  
Joni Leivo ◽  
Sanni Virjula ◽  
Sari Vanhatupa ◽  
Kimmo Kartasalo ◽  
Joose Kreutzer ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cell Attachment ◽  
Collagen Type I ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Type I ◽  
Simple Method ◽  
Dynamic Conditions ◽  
Coating Method ◽  
Cross Linker

Polydimethylsiloxane (PDMS) is widely used in dynamic biological microfluidic applications. As a highly hydrophobic material, native PDMS does not support cell attachment and culture, especially in dynamic conditions. Previous covalent coating methods use glutaraldehyde (GA) which, however, is cytotoxic. This paper introduces a novel and simple method for binding collagen type I covalently on PDMS using ascorbic acid (AA) as a cross-linker instead of GA. We compare the novel method against physisorption and GA cross-linker-based methods. The coatings are characterized by immunostaining, contact angle measurement, atomic force microscopy and infrared spectroscopy, and evaluated in static and stretched human adipose stem cell (hASC) cultures up to 13 days. We found that AA can replace GA as a cross-linker in the covalent coating method and that the coating is durable after sonication and after 6 days of stretching. Furthermore, we show that hASCs attach and proliferate better on AA cross-linked samples compared with physisorbed or GA-based methods. Thus, in this paper, we provide a new PDMS coating method for studying cells, such as hASCs, in static and dynamic conditions. The proposed method is an important step in the development of PDMS-based devices in cell and tissue engineering applications.

scholarly journals 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton’s Jelly Mesenchymal Stem Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1448
Author(s):  
Maria Camilla Ciardulli ◽  
Joseph Lovecchio ◽  
Pasqualina Scala ◽  
Erwin Pavel Lamparelli ◽  
Tina Patricia Dale ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Type I Collagen ◽  
In Vitro Study ◽  
Wharton’S Jelly ◽  
Controlled Delivery ◽  
Type I ◽  
Wharton's Jelly

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

scholarly journals Expression of type I collagen in response to Isoniazid exposure is indirect and is facilitated by collateral induction of cytochrome P450 2E1: An in-vitro study

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0236992
Author(s):  
Suman Santra ◽  
Debasree Bishnu ◽  
Gopal Krishna Dhali ◽  
Amal Santra ◽  
Abhijit Chowdhury
Keyword(s):  
Cytochrome P450 ◽  
Type I Collagen ◽  
In Vitro Study ◽  
Vitro Study ◽  
Type I ◽  
Cytochrome P450 2E1

Effect of Adhesion or Collagen Molecules on Cell Attachment, Insulin Secretion, and Glucose Responsiveness in the Cultured Adult Porcine Endocrine Pancreas: A Preliminary Study

2003 ◽  
Vol 12 (4) ◽  
pp. 439-446 ◽  
Author(s):  
Kazuya Edamura ◽  
Koko Nasu ◽  
Yukiko Iwami ◽  
Hiroyuki Ogawa ◽  
Nobuo Sasaki ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Type I Collagen ◽  
Cell Function ◽  
Cell Attachment ◽  
Control Group ◽  
Type I ◽  
Collagen Group ◽  
Collagen Molecules ◽  
Glucose Responsiveness

The effect of either adhesion or collagen molecules on cell attachment, insulin secretion, and glucose responsiveness was investigated in adult porcine pancreatic endocrine (PE) cells that were cultured for a longer term in vitro. Six different types of molecules—laminin, fibronectin, poly-L-lysine (PLL), type I collagen, gelatin, and Matrigel—were used. Approximately 2.0 × 105 cells per dish of each molecule type were cultured for 4 weeks. In the laminin group, the insulin accumulation was maintained at a significantly higher level than in the control group at 4 weeks of culture, and glucose-stimulated insulin secretion and the insulin-positive rate were also higher than in the control group. In the Matrigel group, islet-like cell clusters were formed, but insulin accumulation rapidly decreased at 3–4 weeks of culture. A large number of PE cells attached tightly and spread in the fibronectin group until the fourth week of culture, but their function was not better than those in the control group. In the PLL and gelatin groups, the PE cell function was not significantly different from that of the control group. In the type I collagen group, insulin secretion was inferior to that of the other groups. The results of this study suggest that laminin is the most suitable extracellular matrix for the long-term culture preservation of PE cells.

Increase in the Biocompatibility of the Neutralized Chitosan Dermal Scaffold by Reconstruction of Wound Healing Microenvironment: In Vitro Study

2007 ◽  
Vol 342-343 ◽  
pp. 185-188 ◽  
Author(s):  
Yong Ha Youn ◽  
Chun Ho Kim ◽  
Young Ju Choi ◽  
Yong Jae Gin ◽  
Young Sook Son
Keyword(s):  
Wound Healing ◽  
Type I Collagen ◽  
Binding Capacity ◽  
In Vitro Study ◽  
Type I ◽  
Cell Binding ◽  
Chitosan Scaffold ◽  
Dry Process ◽  
Wound Healing Effect

The porous neutralized chitosan scaffold (NCS) was prepared by freeze-dry method. Its poor cell binding capacity was improved approximately five folds by mixing or coating of atelomeric type I collagen. In order to recreate wound-healing microenvironment within the NCS for the better wound healing effect, various concentrations of bFGF and fibronectin (FN) were supplied in the secondary freeze-dry process of the scaffold. NCS+ bFGF and NCS+FN improved the cell binding capacity by four folds and three folds respectively. Therefore supplementation of collagen, b-FGF and/or fibronectin in the NCS can improve the biocompatibility of the chitosanbased scaffold which itself revealed poor cell binding capacity.

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