Lactonase activity and status of paraoxonase 1 and oxidative stress in neonates of women with gestational diabetes mellitus

Objective.To determine plasma markers of oxidative stress during the second and third trimester of pregnancy in patients with gestational diabetes mellitus (GDM).Study Design.We conducted a prospective nested case-control study involving 400 pregnant women, 22 of whom developed GDM. As control group, 30 normal pregnant women were chosen randomly. Plasma samples were analyzed for 8-iso-prostaglandin F2α(8-iso-PGF2α), advanced oxidative protein products (AOPPs), protein carbonyl (PCO), glutathione peroxidase-3 (GPX-3), and paraoxonase-1 (PON1) at 16–20 weeks, 24–28 weeks, and 32–36 weeks of gestation.Results.Compared to control subjects, the plasma levels of PCO, AOPPs, and 8-iso-PGF2αwere elevated at 16–20 weeks’ and 32–36 weeks’ gestation in GDM. There was no significant difference in PCO and 8-iso-PGF2αat 24–28 weeks in GDM. GPX-3 was statistically significantly increased at 16–20 weeks and 32–36 weeks in GDM. PON1 reduced in patients with GDM. No significant differences were found at 24–28 and 32–36 weeks between the GDM and control groups. In GDM, PCO, AOPPs, and 8-iso-PGF2αlevels were higher and GPX-3 and PON1 levels were lower in the second than the third trimester.Conclusion.Oxidation status increased in GDM, especially protein oxidation, which may contribute to the pathogenesis of GDM.

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Postpartum development of endothelial dysfunction and oxidative stress markers in women with previous gestational diabetes mellitus

Journal of Endocrinological Investigation ◽

10.1007/s40618-013-0045-6 ◽

2014 ◽

Vol 37 (6) ◽

pp. 503-509 ◽

Cited By ~ 20

Author(s):

M. M. Roca-Rodríguez ◽

C. López-Tinoco ◽

M. Murri ◽

A. Fernández-Deudero ◽

M. V. García-Palacios ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Endothelial Dysfunction ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Previous Gestational Diabetes Mellitus ◽

And Oxidative Stress ◽

Previous Gestational Diabetes

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Lactonase Activity, Status, and Genetic Variations of Paraoxonase 1 in Women with Gestational Diabetes Mellitus

Journal of Diabetes Research ◽

10.1155/2020/3483427 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Mi Zhou ◽

Xing-Hui Liu ◽

Qing-Qing Liu ◽

Meng Chen ◽

Huai Bai ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Genetic Variants ◽

Chinese Women ◽

Paraoxonase 1 ◽

High Density Lipoproteins ◽

Dependent Manner ◽

Calcium Dependent

Background. Paraoxonase 1 (PON1) is a calcium-dependent multifunctional enzyme that binds to high-density lipoproteins. The physiological function of PON1 is related to its lactonase activity. However, this activity has not been analyzed in women with gestational diabetes mellitus (GDM). The present study investigated the lactonase activities and status of PON1 and their association with PON1 genetic variants and oxidative stress indices in Chinese women with GDM. Methods. This is a case-control study of 347 women with GDM and 288 women with uncomplicated pregnancies. PON1 levels and lactonase activities were analyzed using 7-O-diethylphosphoryl-3-cyano-4-methyl-7-hydroxycoumarin (DEPCyMC) and 5-thiobutyl butyrolactone (TBBL), respectively. A normalized lactonase activity (NLA) was estimated based on the ratio of TBBLase to DEPCyMCase activity. Serum malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC) levels, and PON1 genetic polymorphisms were also analyzed. Results. PON1 lactonase activity and levels of TOS, TAC, and MDA were higher in the GDM women compared with the control women. The PON1 -108C→T genetic variation decreased the levels and lactonase activities of PON1 in a genotype-dependent manner in the patient and control groups. GDM patients with the PON1 -108TT genotype displayed lower NLA than those with the -108CC or -108CT genotype. GDM patients with the RR genotype of PON1 192Q/R polymorphism had significantly lower PON1 lactonase activities and NLA and tended to have decreased PON1 levels compared with those with the QQ or QR genotype. Multivariable regression analysis revealed that the PON1 -108C/T or 192Q/R variations, apolipoprotein (apo) A1, apoB, TAC, MDA, or age was significant predictors of the levels, lactonase activities, or NLA of PON1. Conclusions. The lactonase activities of PON1 are increased in women with GDM. PON1 genetic variants, increased oxidative stress, and abnormalities in lipoproteins may be associated with these changes.

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Role of nitrosative and oxidative stress on the development of gestational diabetes mellitus and their effect in GDM placenta

Endocrine Abstracts ◽

10.1530/endoabs.37.ep360 ◽

2015 ◽

Author(s):

Cristina Lopez-Tinoco ◽

Francisco Visiedo ◽

Celeste Rosendo ◽

Maria Belen Ojeda ◽

Isabel Mateos ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

And Oxidative Stress

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Influence of selenium supplementation on carbohydrate metabolism and oxidative stress in pregnant women with gestational diabetes mellitus

Journal of Medical Biochemistry ◽

10.2478/jomb-2019-0034 ◽

2019 ◽

Vol 0 (0) ◽

Author(s):

Hadjer Saifi ◽

Yassine Mabrouk ◽

Rayane Saifi ◽

Messaouda Benabdelkader ◽

Mouldi Saidi

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Pregnant Women ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Good Health ◽

Selenium Supplementation ◽

And Oxidative Stress

Summary Background In the presence of conflicting advice about the relationship between selenium-type II diabetes-oxidative stress trio, this study aimed to assess the consequences of selenium supplementation on fasting plasma glucose (FPG) level, antioxidant activities of selenodependent and non-selenodependent enzymes, and other markers of oxidative stress studied for the first time during gestational diabetes mellitus (GDM). Methods This research was carried out among 180 pregnant Algerian women, 60 of whom were in good health, 60 women with GDM did not take supplements, and 60 women with GDM took selenium orally (50 μg/d) for 12 weeks starting from their second trimester of pregnancy. Blood samples were taken in order to assay FPG level and oxidative stress markers. Results Selenium supplementation during GDM has demonstrated its hypoglycemic power in the significant decline of FPG level, and its antioxidant properties in the significant reinforcement of antioxidant activities of erythrocyte selenodependent enzymes (glutathione peroxidase and glutathione reductase), the significant increase in erythrocyte catalase and superoxide dismutase activities simultaneously with the highest decrease in erythrocyte and plasma malondialdehyde levels. This decrease was only significant for plasma carbonyl proteins, which was not the case for erythrocyte carbonyl proteins. Conclusions The recourse to selenium supplementation by seleno-deficient pregnant women with GDM is beneficial for maternal health. This micronutrient exploits its antioxidant and insulin-mimetic properties in the maintenance of blood glucose homeostasis and the fight against oxidative stress, and consequently, its supplementation delays the occurrence of GDM complications.

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