Proteomic traits vary across taxa in a coastal Antarctic phytoplankton bloom

The ISME Journal ◽

10.1038/s41396-021-01084-9 ◽

2021 ◽

Author(s):

J. Scott P. McCain ◽

Andrew E. Allen ◽

Erin M. Bertrand

Keyword(s):

Ross Sea ◽

Phytoplankton Bloom ◽

Proteome Level ◽

Ocean Study ◽

Regulatory Cost ◽

Trait Inferences ◽

Eukaryotic Microbes ◽

Practical Recommendations ◽

Allocation Strategies

AbstractProduction and use of proteins is under strong selection in microbes, but it is unclear how proteome-level traits relate to ecological strategies. We identified and quantified proteomic traits of eukaryotic microbes and bacteria through an Antarctic phytoplankton bloom using in situ metaproteomics. Different taxa, rather than different environmental conditions, formed distinct clusters based on their ribosomal and photosynthetic proteomic proportions, and we propose that these characteristics relate to ecological differences. We defined and used a proteomic proxy for regulatory cost, which showed that SAR11 had the lowest regulatory cost of any taxa we observed at our summertime Southern Ocean study site. Haptophytes had lower regulatory cost than diatoms, which may underpin haptophyte-to-diatom bloom progression in the Ross Sea. We were able to make these proteomic trait inferences by assessing various sources of bias in metaproteomics, providing practical recommendations for researchers in the field. We have quantified several proteomic traits (ribosomal and photosynthetic proteomic proportions, regulatory cost) in eukaryotic and bacterial taxa, which can then be incorporated into trait-based models of microbial communities that reflect resource allocation strategies.

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A comparison between polynya area and associated ice production with mooring-based measurements of temperature, salinity and currents in the southwestern Ross Sea, Antarctica

Annals of Glaciology ◽

10.3189/172756411795931705 ◽

2011 ◽

Vol 52 (57) ◽

pp. 291-300 ◽

Cited By ~ 3

Author(s):

Stefan Kern ◽

Stefano Aliani

Keyword(s):

Ross Sea ◽

Time Lag ◽

Ice Formation ◽

Terra Nova Bay ◽

In Situ Observations ◽

Satellite Microwave ◽

Terra Nova ◽

Ice Production ◽

The Impact

AbstractWintertime (April–September) area estimates of the Terra Nova Bay polynya (TNBP), Antarctica, based on satellite microwave radiometry are compared with in situ observations of water salinity, temperature and currents at a mooring in Terra Nova Bay in 1996 and 1997. In 1996, polynya area anomalies and associated anomalies in polynya ice production are significantly correlated with salinity anomalies at the mooring. Salinity anomalies lag area and/or ice production anomalies by about 3 days. Up to 50% of the variability in the salinity at the mooring position can be explained by area and/or ice production anomalies in the TNBP for April–September 1996. This value increases to about 70% when considering shorter periods like April–June or May–July, but reduces to 30% later, for example July–September, together with a slight increase in time lag. In 1997, correlations are smaller, less significant and occur at a different time lag. Analysis of ocean currents at the mooring suggests that in 1996 conditions were more favourable than in 1997 for observing the impact of descending plumes of salt-enriched water formed in the polynya during ice formation on the water masses at the mooring depth.

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Use of Microautoradiography Combined with Fluorescence In Situ Hybridization To Determine Dimethylsulfoniopropionate Incorporation by Marine Bacterioplankton Taxa

Applied and Environmental Microbiology ◽

10.1128/aem.70.8.4648-4657.2004 ◽

2004 ◽

Vol 70 (8) ◽

pp. 4648-4657 ◽

Cited By ~ 70

Author(s):

Maria Vila ◽

Rafel Simó ◽

Ronald P. Kiene ◽

Jarone Pinhassi ◽

José M. González ◽

...

Keyword(s):

In Situ Hybridization ◽

Gulf Of Mexico ◽

Fluorescence In Situ Hybridization ◽

Marine Bacteria ◽

Heterotrophic Bacteria ◽

Phytoplankton Bloom ◽

Mediterranean Coast ◽

Marine Bacterioplankton ◽

Significant Uptake

ABSTRACT The fraction of planktonic heterotrophic bacteria capable of incorporating dissolved dimethylsulfoniopropionate (DMSP) and leucine was determined at two coastal sites by microautoradioagraphy (AU). In Gulf of Mexico seawater microcosm experiments, the proportion of prokaryotes that incorporated sulfur from [35S]DMSP ranged between 27 and 51% of 4′,6-diamidino-2-phenylindole (DAPI)-positive cells, similar to or slightly lower than the proportion incorporating [3H]leucine. In the northwest Mediterranean coast, the proportion of cells incorporating sulfur from [35S]DMSP increased from 5 to 42% from January to March, coinciding with the development of a phytoplankton bloom. At the same time, the proportion of cells incorporating [3H]leucine increased from 21 to 40%. The combination of AU and fluorescence in situ hybridization (FISH) revealed that the Roseobacter clade (α-proteobacteria) accounted for 13 to 43% of the microorganisms incorporating [35S]DMSP at both sampling sites. Significant uptake of sulfur from DMSP was also found among members of the γ-proteobacteria and Cytophaga-Flavobacterium groups. Roseobacter and γ-proteobacteria exhibited the highest percentage of DAPI-positive cells incorporating 35S from DMSP (around 50%). Altogether, the application of AU with [35S]DMSP combined with FISH indicated that utilization of S from DMSP is a widespread feature among active marine bacteria, comparable to leucine utilization. These results point toward DMSP as an important substrate for a broad and diverse fraction of marine bacterioplankton.

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Anomalous wind triggered the largest phytoplankton bloom in the oligotrophic North Pacific Subtropical Gyre

Scientific Reports ◽

10.1038/s41598-019-51989-x ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Chun Hoe Chow ◽

Wee Cheah ◽

Jen-Hua Tai ◽

Sin-Fu Liu

Keyword(s):

North Pacific ◽

Phytoplankton Bloom ◽

Subtropical Gyre ◽

Velocity Shear ◽

North Pacific Subtropical Gyre ◽

The North ◽

Large Eddy ◽

Anomalous Wind ◽

Anticyclonic Eddies

Abstract In summer 2010, a massive bloom appeared in the middle (16–25°N, 160–200°E) of the North Pacific Subtropical Gyre (NPSG) creating a spectacular oasis in the middle of the largest oceanic desert on Earth. Peaked in June 2010 covering over two million km2 in space, this phytoplankton bloom is the largest ever recorded by ocean color satellites in the NPSG over the period from 1997 to 2013. The initiation and mechanisms sustaining the massive bloom were due to atmospheric and oceanic anomalies. Over the north (25–30°N) of the bloom, strong anticyclonic winds warmed sea surface temperature (SST) via Ekman convergence. Subsequently, anomalous westward ocean currents were generated by SST meridional gradients between 19°N and 25°N, producing strong velocity shear that caused large number of mesoscale (100-km in order) cyclonic eddies in the bloom region. The ratio of cyclonic to anticyclonic eddies of 2.7 in summer 2010 is the highest over the 16-year study period. As a result of the large eddy-number differences, eddy-eddy interactions were strong and induced submesoscale (smaller than 100 km) vertical pumping as observed in the in-situ ocean profiles. The signature of vertical pumping was also presented in the in-situ measurements of chlorophyll and nutrients, which show higher concentrations in 2010 than other years.

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High-resolution spatio-temporal distribution of a coastal phytoplankton bloom using laser in situ scattering and transmissometry (LISST)

Harmful Algae ◽

10.1016/j.hal.2008.04.004 ◽

2008 ◽

Vol 7 (6) ◽

pp. 808-816 ◽

Cited By ~ 32

Author(s):

S. Anglès ◽

A. Jordi ◽

E. Garcés ◽

M. Masó ◽

G. Basterretxea

Keyword(s):

High Resolution ◽

Phytoplankton Bloom ◽

Temporal Distribution ◽

Spatio Temporal ◽

Coastal Phytoplankton ◽

In Situ Scattering

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Surface layer variability in the Ross Sea, Antarctica as assessed by in situ fluorescence measurements

Progress In Oceanography ◽

10.1016/j.pocean.2010.08.002 ◽

2011 ◽

Vol 88 (1-4) ◽

pp. 28-45 ◽

Cited By ~ 30

Author(s):

Walker O. Smith ◽

Vernon Asper ◽

Sasha Tozzi ◽

Xiao Liu ◽

Sharon E. Stammerjohn

Keyword(s):

Surface Layer ◽

Ross Sea ◽

Fluorescence Measurements

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Summer and early-fall Sea-ice concentration in the Ross Sea: comparison of in Situ ASPeCt observations and satellite passive microwave estimates

Annals of Glaciology ◽

10.3189/172756406781811466 ◽

2006 ◽

Vol 44 ◽

pp. 303-309 ◽

Cited By ~ 18

Author(s):

Margaret A. Knuth ◽

Stephen F. Ackley

Keyword(s):

Sea Ice ◽

Satellite Data ◽

Ross Sea ◽

Passive Microwave ◽

Sea Ice Concentration ◽

Point Sampling ◽

Continuous Sampling ◽

Ice Concentration ◽

Observation Protocol

AbstractSea-ice conditions were observed using the AsPeCt observation protocol on three cruises in the Ross Sea spanning the Antarctic Summer Season (APIs, December 1999–February 2000; Anslope 1, March–April 2003; Anslope 2, February–April 2004). An additional dataset was analyzed from helicopter video Surveys taken during the APIs cruise. The helicopter video was analyzed using two techniques: first, as an AsPeCt dataset where it was Sampled visually for ice concentration, floe Sizes and ice type on a point basis at 11 km intervals; Second, computerized image processing on a Subset of nine helicopter flights to obtain ice concentration on a continuous basis (1 S intervals) for the entire flight. This continuous Sampling was used to validate the point-sampling methods to characterize the ice cover; the ‘AsPeCt Sampling’ on the helicopter video and the use of the AsPeCt protocol on the Ship Surveys. The estimates for average ice concentration agreed within 5% for the continuous digitized data and point Sampling at 11 km intervals in this comparison. The Ship and video in Situ datasets were then compared with ice concentrations from SsM/I passive microwave Satellite data derived using the Bootstrap and NAsA-Team algorithms. Less than 50% of the variance in Summer ice concentration observed in Situ was explainable by Satellite microwave data. The Satellite data were also inconsistent in measurement, both underestimating and overestimating the concentration for Summer conditions, but improved in the fall period when conditions were colder. This improvement was in the explainable variance of >70%, although in Situ concentration was underestimated (albeit consistently) by the Satellite imagery in fall.

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Changes in Heterotrophic Picoplankton Community Structure after Induction of a Phytoplankton Bloom under Different Light Regimes

Diversity ◽

10.3390/d11100195 ◽

2019 ◽

Vol 11 (10) ◽

pp. 195 ◽

Cited By ~ 1

Author(s):

Karayanni ◽

Kormas ◽

Moustaka-Gouni ◽

Sommer

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Relative Abundance ◽

Environmental Changes ◽

Phytoplankton Bloom ◽

Phytoplankton Growth ◽

Light Regimes ◽

Operational Taxonomic Units ◽

Abundant Otus

Bacterial and archaeal diversity and succession were studied during a mesocosm experiment that investigated whether changing light regimes could affect the onset of phytoplankton blooms. For this, 454-pyrosequencing of the bacterial V1-V3 and archaeal V3-V9 16S rRNA regions was performed in samples collected from four mesocosms receiving different light irradiances at the beginning and the end of the experiment and during phytoplankton growth. In total, 46 bacterial operational taxonomic units (OTUs) with ≥1% relative abundance occurred (22–34 OTUs per mesocosm). OTUs were affiliated mainly with Rhodobacteraceae, Flavobacteriaceae and Alteromonadaceae. The four mesocosms shared 11 abundant OTUs. Dominance increased at the beginning of phytoplankton growth in all treatments and decreased thereafter. Maximum dominance was found in the mesocosms with high irradiances. Overall, specific bacterial OTUs had different responses in terms of relative abundance under in situ and high light intensities, and an early phytoplankton bloom resulted in different bacterial community structures both at high (family) and low (OTU) taxonomic levels. Thus, bacterial community structure and succession are affected by light regime, both directly and indirectly, which may have implications for an ecosystem’s response to environmental changes.

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Swimming in the Antarctic scallop Adamussium colbecki: analysis of in situ video recordings

Antarctic Science ◽

10.1017/s0954102098000455 ◽

1998 ◽

Vol 10 (4) ◽

pp. 369-375 ◽

Cited By ~ 30

Author(s):

Alan D. Ansell ◽

Riccardo Cattaneo-Vietti ◽

Mariachiara Chiantore

Keyword(s):

Ross Sea ◽

Maximum Velocity ◽

Remotely Operated Vehicle ◽

Mean Velocity ◽

Terra Nova Bay ◽

Video Recordings ◽

Swimming Trajectories ◽

Opening And Closing ◽

The Antarctic

Swimming activity of the Antarctic scallop at a temperature of c. −1.4°C was analysed using in situ video recordings obtained from a remotely operated vehicle (ROV) in Terra Nova Bay, Ross Sea. Data are presented on swimming trajectories, distance travelled and velocity during a swimming bout, adduction frequency, shell gape angle, and the angular opening and closing velocities of shell. This scallop is an effective swimmer although swimming bouts in response to the ROV were generally short, consisting of 2–5 adduction cycles in the take-off phase followed by 1–4 (exceptionally 14) adductions during level swimming. The maximum velocity during each adduction cycle ranged from 19.4–43.1 cm s−1 and the mean velocity during a swimming bout from 12.0–23.5 cm s−1. Each adduction cycle consists of opening, closing and glide phases of approximately equal duration. Adduction frequency during swimming averaged 1.5 adductions s−1.

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A model for the spreading and sinking of the Deep Ice Shelf Water in the Ross Sea

Antarctic Science ◽

10.1017/s0954102003001020 ◽

2003 ◽

Vol 15 (1) ◽

pp. 25-30 ◽

Cited By ~ 14

Author(s):

ANGELO RUBINO ◽

GIORGIO BUDILLON ◽

STEFANO PIERINI ◽

GIANCARLO SPEZIE

Keyword(s):

Ross Sea ◽

Deep Ocean ◽

Ocean Dynamics ◽

Shelf Water ◽

Density Structure ◽

Ice Shelf ◽

Ice Shelf Water ◽

Realistic Topography ◽

Good Agreement

Spreading and sinking of the Deep Ice Shelf Water (DISW) in the Ross Sea are analysed using in situ observations and the results of a nonlinear, reduced gravity, layered numerical model, which is able to simulate the motion of a bottom trapped current over realistic topography. The model is forced by prescribing thickness and density of the DISW layer at the southern model boundary as well as ambient density stratification above the DISW layer. This density structure is imposed using hydrographic data acquired by the Italian PNRA-CLIMA project. In the model water of the quiescent ambient ocean is allowed to entrain in the active deep layer due to a simple entrainment parameterization. The importance of forcing the model with a realistic ambient density is demonstrated by carrying out a numerical simulation using an idealized ambient density. In a more realistic simulation the path and the density structure of the DISW vein flowing over the Challenger Basin are obtained and are found to be in good agreement with data. It is found that entrainment, which is particularly active in regions of strong topographic variation, significantly influences the pattern followed by the DISW layer. The evolution of the DISW layer beyond the continental shelf, i.e., in a region where the paucity of experimental data does not allow for a detailed description of the deep ocean dynamics, is also investigated.

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