Comparison of solithromycin with erythromycin in Enterococcus faecalis and Enterococcus faecium from China: antibacterial activity, clonality, resistance mechanism, and inhibition of biofilm formation

This study investigated eight types of Slovak dry fermented meat products (salami and sausages) that are available on the market and were produced by three different producers in different regions of Slovakia. The total counts of enterococci in these products ranged from 2.0 up to 6.0 cfu/g (log10). Three species were identified among the 15 selected enterococcal strains; Enterococcus faecium (8 strains), Enterococcus faecalis (3) and Enterococcus hirae (4). They were hemolysis-negative (γ-hemolysis) with a biofilm-forming ability, which was evaluated as low-grade biofilm formation, susceptible to conventional antibiotics and mainly susceptible to lantibiotic bacteriocins, namely, gallidermin and nisin; they even showed a higher susceptibility to gallidermin than to nisin. They were also susceptible to enterocin–durancin, but most strains showed resistance to enterocin A/P. This study indicated that bacteriocins can play a key role in preventing and/or protecting from undesirable bacterial multiplication or contamination in the food industry and that they have great potential for further experimental applications.

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Importance of Enterococcus spp. for Forming a Biofilm

Czech Journal of Food Sciences ◽

10.17221/1087-cjfs ◽

2009 ◽

Vol 27 (Special Issue 1) ◽

pp. S354-S356 ◽

Cited By ~ 5

Author(s):

L. Necidová ◽

B. Janštová ◽

S. Karpíšková ◽

Š. Cupáková ◽

M. Dušková ◽

...

Keyword(s):

Enterococcus Faecalis ◽

Enterococcus Faecium ◽

Biofilm Formation ◽

Dairy Products ◽

Formation Potential ◽

Farm Level ◽

Milk Samples ◽

Bulk Milk ◽

Dairy Plant ◽

Plant Level

The aim of this study was to monitore the capability of Enterococcus fecalis and Enterococcus fecium to form biofilms. Enterococci isolates originated from individual milk, bulk milk samples and environmental swabs obtained at farm level, dairy plant level including semi and final dairy products. Biofilm formation potential was determined by growing the tested strains in glas tubes containing BHI medium. The capability of forming biofilms was detected in 28% of Enterococcus spp. strains. Higher number of biofilm forming strains of the Enterococcus faecium (33%) than Enterococcus faecalis (28%) has been registered. Isolates obtained at plant level were forming biofilms more often than isolates from plant level and in final products (cheese and curd cheese), no isolate has been seen to be able to form biofilm.

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Detection of virulence factors in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolates from a Tunisian hospital

Canadian Journal of Microbiology ◽

10.1139/w06-136 ◽

2007 ◽

Vol 53 (3) ◽

pp. 372-379 ◽

Cited By ~ 17

Author(s):

N. Klibi ◽

K. Ben Slama ◽

Y. Sáenz ◽

A. Masmoudi ◽

S. Zanetti ◽

...

Keyword(s):

Enterococcus Faecalis ◽

Virulence Factors ◽

Enterococcus Faecium ◽

Biofilm Formation ◽

Virulence Genes ◽

Virulence Gene ◽

Gelatinase Activity ◽

Genes Encoding ◽

High Level

Phenotypic and genotypic determination of virulence factors were carried out in 46 high-level gentamicin-resistant (HLGR) clinical Enterococcus faecalis (n = 34) and Enterococcus faecium (n = 12) isolates recovered from different patients in La Rabta Hospital in Tunis, Tunisia, between 2000 and 2003 (all these isolates harboured the aac(6′)–aph(2″) gene). The genes encoding virulence factors (agg, gelE, ace, cylLLS, esp, cpd, and fsrB) were analysed by PCR and sequencing. The production of gelatinase and hemolysin, the adherence to caco-2 and hep-2 cells, and the capacity for biofilm formation were investigated in all 46 HLGR enterococci. The percentages of E. faecalis isolates harbouring virulence genes were as follows: gelE, cpd, and ace (100%); fsrB (62%); agg (56%); cylLLS (41.2%); and esp (26.5%). The only virulence gene detected among the 12 HLGR E. faecium isolates was esp (58%). Gelatinase activity was detected in 22 of the 34 E. faecalis isolates (65%, most of them with the gelE+–fsrB+ genotype); the remaining 12 isolates were gelatinase-negative (with the gelE+–fsrB– genotype and the deletion of a 23.9 kb fragment of the fsr locus). Overall, 64% of the cylLLS-containing E. faecalis isolates showed β-hemolysis. A high proportion of our HLGR E. faecalis isolates, in contrast to E. faecium, showed moderate or strong biofilm formation or adherence to caco-2 and hep-2 cells.

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Antibacterial Activity against Enterococcus faecium, Enterococcus faecalis and Inhibitory Activity of Monoamine Oxidase A & B by Persea americana “Avocado” Seed Extracts

Asian Journal of Chemistry ◽

10.14233/ajchem.2021.23294 ◽

2021 ◽

Vol 33 (9) ◽

pp. 2093-2098

Author(s):

N-D. Nogbou ◽

Z.T. Mkhombo ◽

M.T. Olivier ◽

M.O. Ogunrombi ◽

R.B. Maseko ◽

...

Keyword(s):

Antibacterial Activity ◽

Monoamine Oxidase ◽

Enterococcus Faecalis ◽

Inhibitory Activity ◽

Enterococcus Faecium ◽

Persea Americana ◽

Monoamine Oxidase A ◽

Resistant Bacteria ◽

Mao A ◽

Seed Extracts

This study reported various Persia americana seed extracts for the presence and total content of phytochemicals, antibacterial activity and inhibitory activity against monoamine oxidase (MAO) A & B enzymes. Phytochemical studies showed that phenols, flavonoids and tannins were found in all the polar solvents. The highest antibacterial activity was exhibited against Gram-positive bacteria by acetone extract on Enterococcus faecium and methanol extract on Enterococcus faecalis. From the monoamine inhibition experiments, a significant inhibitory activity was observed from ethanolic extracts against MAO-A and from n-hexane extracts against MAO-B. This study demonstrates the antimicrobial activity of P. americana seed extracts against enterococci bacteria, E. faecium and E. faecalis. This finding offers hope of a potential new antibacterial compound in the treatment of these multidrug resistant bacteria.

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Enterococcus faeciumWB2000 Inhibits Biofilm Formation by Oral Cariogenic Streptococci

International Journal of Dentistry ◽

10.1155/2011/834151 ◽

2011 ◽

Vol 2011 ◽

pp. 1-5 ◽

Cited By ~ 11

Author(s):

Nao Suzuki ◽

Masahiro Yoneda ◽

Yuko Hatano ◽

Tomoyuki Iwamoto ◽

Yosuke Masuo ◽

...

Keyword(s):

Streptococcus Mutans ◽

Enterococcus Faecalis ◽

Enterococcus Faecium ◽

Biofilm Formation ◽

Inhibitory Effect ◽

Mutans Streptococci ◽

Streptococcus Sobrinus ◽

Dual Cultures

This study investigated the inhibitory effect of probioticEnterococcus faeciumWB2000 on biofilm formation by cariogenic streptococci. The ability ofE. faeciumWB2000 and JCM5804 andEnterococcus faecalisJCM5803 to inhibit biofilm formation by seven laboratory oral streptococcal strains and 13 clinical mutans streptococcal strains was assayed. TheEnterococcalstrains inhibited biofilm formation in dual cultures with the mutans streptococcal strainsStreptococcus mutansXc andStreptococcus sobrinusJCM5176 (P<0.05), but not with the noncariogenic streptococcal strains.Enterococcus faeciumWB2000 inhibited biofilm formation by 90.0% (9/10) of the clinicalS. mutansstrains and 100% (3/3) of the clinicalS. sobrinusstrains. After culturing, the pH did not differ between single and dual cultures. The viable counts of floating mutans streptococci were lower in dual cultures withE. faeciumWB2000 than in single cultures.Enterococcus faeciumWB2000 acted as a probiotic bacterial inhibitor of cariogenic streptococcal biofilm formation.

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