scholarly journals Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Hossein Mohammad-Beigi ◽  
Yuya Hayashi ◽  
Christina Moeslund Zeuthen ◽  
Hoda Eskandari ◽  
Carsten Scavenius ◽  
...  
Keyword(s):  
Weak Interactions ◽  
Protein Corona ◽  
Dynamic Nature ◽  
Polystyrene Nanoparticles ◽  
Hard Component ◽  
Cell Association ◽  
Differential Binding ◽  
Analytical Separation ◽  
Biological Milieu

Abstract The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

scholarly journals Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

2020 ◽  
Author(s):  
Hossein Mohammad-Beigi ◽  
Yuya Hayashi ◽  
Christina Moeslund Zeuthen ◽  
Hoda Eskandari ◽  
Carsten Scavenius ◽  
...  
Keyword(s):  
Weak Interactions ◽  
Protein Corona ◽  
Interacting Proteins ◽  
Polystyrene Nanoparticles ◽  
Hard Component ◽  
Cell Association ◽  
Differential Binding ◽  
Analytical Separation ◽  
Biological Milieu

AbstractThe current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identified a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins in the SC are revealed as modulators of nanoparticle-cell association, in spite of their short residence time. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

scholarly journals In situ analysis of liposome hard and soft protein corona structure and composition in a single label-free workflow

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 1728-1741 ◽  
Author(s):  
Otto K. Kari ◽  
Joseph Ndika ◽  
Petteri Parkkila ◽  
Antti Louna ◽  
Tatu Lajunen ◽  
...  
Keyword(s):  
Structural Properties ◽  
Protein Corona ◽  
In Situ Analysis ◽  
Label Free ◽  
Holistic Understanding ◽  
Corona Structure

Towards holistic understanding of biological identity: combining corona subsection structural properties with proteomics compositions obtained non-invasively in physiologically relevant conditions.

scholarly journals Nanoparticles-cell association predicted by protein corona fingerprints

Nanoscale ◽  
2016 ◽  
Vol 8 (25) ◽  
pp. 12755-12763 ◽  
Author(s):  
S. Palchetti ◽  
L. Digiacomo ◽  
D. Pozzi ◽  
G. Peruzzi ◽  
E. Micarelli ◽  
...  
Keyword(s):  
Protein Corona ◽  
Cell Association

scholarly journals Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium

2017 ◽  
Vol 121 (2) ◽  
pp. 345-358
Author(s):  
Olivier Leroux ◽  
Michaela Eder ◽  
Friederike Saxe ◽  
John W C Dunlop ◽  
Zoë A Popper ◽  
...  
Keyword(s):  
Cell Walls ◽  
In Situ Analysis ◽  
Dynamic Nature

scholarly journals Inside-Out Regulation of L1 Conformation, Integrin Binding, Proteolysis, and Concomitant Cell Migration

2010 ◽  
Vol 21 (10) ◽  
pp. 1671-1685 ◽  
Author(s):  
Maxine M. Chen ◽  
Chia-Yao Lee ◽  
Hyuma A. Leland ◽  
Grace Y. Lin ◽  
Anthony M. Montgomery ◽  
...  
Keyword(s):  
Cell Migration ◽  
Ductal Adenocarcinoma ◽  
Cell Phenotype ◽  
Poorly Differentiated ◽  
Differential Binding ◽  
A Disintegrin And Metalloproteinase ◽  
Cell Adhesion Molecule L1 ◽  
Inside Out

Previous reports on the expression of the cell adhesion molecule L1 in pancreatic ductal adenocarcinoma (PDAC) cells range from absent to high. Our data demonstrate that L1 is expressed in poorly differentiated PDAC cells in situ and that threonine-1172 (T1172) in the L1 cytoplasmic domain exhibits steady-state saturated phosphorylation in PDAC cells in vitro and in situ. In vitro studies support roles for casein kinase II and PKC in this modification, consistent with our prior studies using recombinant proteins. Importantly, T1172 phosphorylation drives, or is associated with, a change in the extracellular structure of L1, consistent with a potential role in regulating the shift between the closed conformation and the open, multimerized conformation of L1. We further demonstrate that these distinct conformations exhibit differential binding to integrins αvβ3 and αvβ5 and that T1172 regulates cell migration in a matrix-specific manner and is required for a disintegrin and metalloproteinase-mediated shedding of the L1 ectodomain that has been shown to regulate cell migration. These data define a specific role for T1172 of L1 in regulating aspects of pancreatic adenocarcinoma cell phenotype and suggest the need for further studies to elucidate the specific ramifications of L1 expression and T1172 phosphorylation in the pathobiology of pancreatic cancer.

Dynamic Changes in the Distribution of a Satellite Homologous to Intergenic 26-18S rDNA Spacer in the Evolution of Nicotiana

Genetics ◽  
2004 ◽  
Vol 166 (4) ◽  
pp. 1935-1946
Author(s):  
K Y Lim ◽  
K Skalicka ◽  
B Koukalova ◽  
R A Volkov ◽  
R Matyasek ◽  
...  
Keyword(s):  
18S Rdna ◽  
Intergenic Spacer ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Dynamic Nature ◽  
Dynamic Changes ◽  
Nicotiana Tomentosiformis ◽  
Copy Numbers ◽  
Location Patterns

Abstract An ∼135-bp sequence called the A1/A2 repeat was isolated from the transcribed region of the 26-18S rDNA intergenic spacer (IGS) of Nicotiana tomentosiformis. Fluorescence in situ hybridization (FISH) and Southern blot analysis revealed its occurrence as an independent satellite (termed an A1/A2 satellite) outside of rDNA loci in species of Nicotiana section Tomentosae. The chromosomal location, patterns of genomic dispersion, and copy numbers of its tandemly arranged units varied between the species. In more distantly related Nicotiana species the A1/A2 repeats were found only at the nucleolar organizer regions (NOR). There was a trend toward the elimination of the A1/A2 satellite in N. tabacum (tobacco), an allotetraploid with parents closely related to the diploids N. sylvestris and N. tomentosiformis. This process may have already commenced in an S3 generation of synthetic tobacco. Cytosine residues in the IGS were significantly hypomethylated compared with the A1/A2 satellite. There was no clear separation between the IGS and satellite fractions in sequence analysis of individual clones and we found no evidence for CG suppression. Taken together the data indicate a dynamic nature of the A1/A2 repeats in Nicotiana genomes, with evidence for recurrent integration, copy number expansions, and contractions.

The in situ relationships between season of hatching, growth and condition in the southern calamary, Sepioteuthis australis

2004 ◽  
Vol 55 (4) ◽  
pp. 429 ◽  
Author(s):  
Gretta Pecl
Keyword(s):  
East Coast ◽  
Average Rate ◽  
Seasonal Patterns ◽  
Annual Species ◽  
Dynamic Nature ◽  
Growth Season ◽  
Adult Growth ◽  
Relationship Of ◽  
Rates Of Growth

This paper examines seasonal patterns in growth and condition of Sepioteuthis australis from temperate waters of Tasmania, Australia. Growth was described by a power function and was fast for a temperate species, with an average rate over the lifetime of 4–5% BW day–1. Sepioteuthis australis is an annual species, however spawning and hatching of juveniles appears to occur all year round. Analysis of individual juvenile growth demonstrated a correlation between seasonally increasing temperatures and progressively faster growth. Season of hatching also had a clear effect on adult growth; summer-hatched individuals were larger at 170–190 days of age compared with winter-hatched individuals (1002 ± 98 g and 632 ± 27 g respectively). The length–mantle weight relationship of adults was also dependent on season of hatching, with individuals hatched in summer and spring having heavier mantles at a given length than those hatched in winter or autumn. Differential rates of growth or varying levels of condition, or perhaps both, may affect the survivorship of individuals. Growth, condition and potentially lifespan of S. australis are dependent on environmental factors, with the dynamic nature of oceanographical conditions on the east coast of Tasmania resulting in a highly variable and fluctuating population structure.

scholarly journals Particle-by-Particle in Situ Characterization of the Protein Corona via Real-Time 3D Single Particle Tracking Microscopy

2021 ◽  
Author(s):  
Xiaochen Tan ◽  
Kevin Welsher
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Fluids ◽  
Protein Corona ◽  
Single Particle Tracking ◽  
Ex Situ ◽  
High Background ◽  
Particle Nature

<p>Nanoparticles (NPs) adsorb proteins when exposed to biological fluids, forming a dynamic protein corona that affects their fate in biological environments. A comprehensive understanding of the protein corona is lacking due to the inability of current techniques to precisely measure the full corona <i>in situ</i> at the single particle level. Herein, we introduce a 3D real-time single-particle tracking spectroscopy to "lock-on" to single freely-diffusing polystyrene NPs and probe their individual protein coronas. The diffusive motions of the tracked NPs enable quantification of the "hard corona" using mean-squared displacement analysis. Critically, this method's particle-by-particle nature enabled a lock-in-type frequency filtering approach to extract the full protein corona, despite the typically confounding effect of high background signal from unbound proteins. From these results, the dynamic <i>in situ </i>full protein corona is observed to contain double the number of proteins than are observed in the <i>ex situ</i> measured "hard" protein corona.</p><br>

scholarly journals Insights into Characterization Methods and Biomedical Applications of Nanoparticle–Protein Corona

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3093
Author(s):  
Yan Li ◽  
Jae-Seung Lee
Keyword(s):  
Biomedical Applications ◽  
Protein Corona ◽  
Living Systems ◽  
Future Perspectives ◽  
Research Attention ◽  
Characterization Methods ◽  
Biological Environment ◽  
Biological Milieu

Nanoparticles (NPs) exposed to a biological milieu will strongly interact with proteins, forming “coronas” on the surfaces of the NPs. The protein coronas (PCs) affect the properties of the NPs and provide a new biological identity to the particles in the biological environment. The characterization of NP-PC complexes has attracted enormous research attention, owing to the crucial effects of the properties of an NP-PC on its interactions with living systems, as well as the diverse applications of NP-PC complexes. The analysis of NP-PC complexes without a well-considered approach will inevitably lead to misunderstandings and inappropriate applications of NPs. This review introduces methods for the characterization of NP-PC complexes and investigates their recent applications in biomedicine. Furthermore, the review evaluates these characterization methods based on comprehensive critical views and provides future perspectives regarding the applications of NP-PC complexes.

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