scholarly journals Rheology of rounded mammalian cells over continuous high-frequencies

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Gotthold Fläschner ◽  
Cosmin I. Roman ◽  
Nico Strohmeyer ◽  
David Martinez-Martin ◽  
Daniel J. Müller
Keyword(s):  
High Frequency ◽  
Mammalian Cells ◽  
Mechanical Response ◽  
Cell Mass ◽  
Low Frequency ◽  
Mass Measurements ◽  
High Frequencies ◽  
Polymer Relaxation ◽  
Continuous Frequency ◽  
Rheological Experiments

AbstractUnderstanding the viscoelastic properties of living cells and their relation to cell state and morphology remains challenging. Low-frequency mechanical perturbations have contributed considerably to the understanding, yet higher frequencies promise to elucidate the link between cellular and molecular properties, such as polymer relaxation and monomer reaction kinetics. Here, we introduce an assay, that uses an actuated microcantilever to confine a single, rounded cell on a second microcantilever, which measures the cell mechanical response across a continuous frequency range ≈ 1–40 kHz. Cell mass measurements and optical microscopy are co-implemented. The fast, high-frequency measurements are applied to rheologically monitor cellular stiffening. We find that the rheology of rounded HeLa cells obeys a cytoskeleton-dependent power-law, similar to spread cells. Cell size and viscoelasticity are uncorrelated, which contrasts an assumption based on the Laplace law. Together with the presented theory of mechanical de-embedding, our assay is generally applicable to other rheological experiments.

scholarly journals ANALYSIS OF HIGH-FREQUENCY C-CORE MAGNETIC FLUX LEAKAGES FOR BONE TUMOR WITH INDUCTION HEATING BY USING MULTI-COIL

2019 ◽  
Author(s):  
Metharak Jokpudsa ◽  
Supawat Kotchapradit ◽  
Chanchai Thongsopa ◽  
Thanaset Thosdeekoraphat
Keyword(s):  
Magnetic Field ◽  
High Frequency ◽  
Tumor Tissue ◽  
Low Frequency ◽  
Heat Energy ◽  
High Frequencies ◽  
Frequency Magnetic Field ◽  
High Frequency Magnetic Field ◽  
The Magnetic Field ◽  
Flux Leakage

High-frequency magnetic field has been developed pervasively. The induction of heat from the magnetic field can help to treat tumor tissue to a certain extent. Normally, treatment by the low-frequency magnetic field needed to be combined with magnetic substances. To assist in the induction of magnetic fields and reduce flux leakage. However, there are studies that have found that high frequencies can cause heat to tumor tissue. In this paper present, a new magnetic application will focus on the analysis of the high-frequency magnetic nickel core with multi-coil. In order to focus the heat energy using a high-frequency magnetic field into the tumor tissue. The magnetic coil was excited by 915 MHz signal and the combination of tissues used are muscle, bone, and tumor. The magnetic power on the heating predicted by the analytical model, the power loss density (2.98e-6 w/m3) was analyzed using the CST microwave studio.

An Oscillating Disk Rheometer for Measuring Dynamic Properties during Vulcanization

1963 ◽  
Vol 36 (2) ◽  
pp. 451-458 ◽  
Author(s):  
G. E. Decker ◽  
R. W. Wise ◽  
D. Guerry
Keyword(s):  
Small Angle ◽  
High Frequency ◽  
Unique Feature ◽  
Dynamic Properties ◽  
Dynamic Stiffness ◽  
Low Frequency ◽  
Stress And Strain ◽  
Loss Angle ◽  
High Frequencies ◽  
Rubber Specimen

Abstract A forced oscillating-disk rheometer has been developed which can measure both the low and high frequency dynamic properties of a rubber specimen throughout vulcanization. It may also be used to measure the rheological properties of unvulcanized polymers. The instrument consists of a cone-shaped disk which is embedded in the rubber specimen and oscillated through a small angle while the specimen is heated under pressure. Both stress and strain in terms of torque and displacement, respectively, are measured by appropriate transducers and recorded on an oscillograph. Provisions are made for changing both frequency and strain. At low frequency, the instrument is a convenient tool for determining all of the curing parameters of a rubber specimen. At high frequencies, the change in the dynamic properties of a rubber specimen throughout vulcanization may be continuously followed. A unique feature of the rheometer is that the loss angle may be determined which permits resolution of the measured dynamic stiffness into its elastic and viscous components.

Mechanisms of Noise Transmission through Helicopter Gearbox Support Struts

1994 ◽  
Vol 116 (4) ◽  
pp. 548-554 ◽  
Author(s):  
M. J. Brennan ◽  
R. J. Pinnington ◽  
S. J. Elliott
Keyword(s):  
High Frequency ◽  
Vibration Isolation ◽  
Low Frequency ◽  
Structural Members ◽  
Vibration Transmission ◽  
Gear Noise ◽  
High Frequencies ◽  
Passive Vibration Isolation ◽  
In Series ◽  
Frequency Behavior

Helicopter gearbox support struts are structural members designed to take large compressive and tensile mechanical loads. They also act as vibration transmission paths connecting the major noise and vibration generators of the main rotor and gearbox to the fuselage and cabin. In this paper the mechanisms of vibration transmission through these struts are examined. The differences between the low frequency behavior that is important in the transmission of the rotor vibrations, and the high frequency behavior that is important in the transmission of gear noise are discussed. A flexible element is introduced in series with the strut, and the limitations of this simple passive measure in attenuating longitudinal and flexural vibrations are highlighted. The analysis shows that there is a difference in the mechanisms of vibration transmission between low and high frequencies, and that there are difficulties in applying effective passive vibration isolation measures to this system.

scholarly journals High-frequency Turnover in Pulsar Spectra

2004 ◽  
Vol 218 ◽  
pp. 339-340 ◽  
Author(s):  
J. Kijak ◽  
O. Maron
Keyword(s):  
High Frequency ◽  
Low Frequency ◽  
High Frequencies

Spectra of several pulsars with turnover at unusually high frequencies ∼ 1 GHz are presented. These are relatively young pulsars with DM typically larger than for pulsars showing a low-frequency turnover.

scholarly journals A possible mechanism for flattening of pulsar spectra at high frequencies

2000 ◽  
Vol 177 ◽  
pp. 395-396
Author(s):  
I.F. Malov
Keyword(s):  
Spectral Index ◽  
High Frequency ◽  
Linear Part ◽  
Low Frequency ◽  
Usual Model ◽  
High Frequencies ◽  
Unusual Behaviour ◽  
Curvature Radiation

Pulsar spectra have three characteristic parts: a low frequency turnover (the region of the maximum), a linear part with a constant spectral index, and a high frequency cut-off (after it the spectrum becomes considerably steeper) (Malofeev et al. 1994). These three parts can be described in the frame of the usual model of curvature radiation (Malov 1979; Ochelkov & Usov 1984; Kuz’min & Solov’ev 1986; Malov & Malofeev 1991). Kramer et al. (1997) have found a flattening in the spectra of 4 pulsars at frequencies above 30 GHz. It cannot be understood in such model. We propose the possible explanation for the unusual behaviour of these spectra.

Electroencephalographic and blood parameters changes in anaesthetised goats subjected to slaughter without stunning and slaughter following different electrical stunning methods

2019 ◽  
Vol 59 (5) ◽  
pp. 849
Author(s):  
A. B. Sabow ◽  
Y. M. Goh ◽  
I. Zulkifli ◽  
M. Z. Ab Kadir ◽  
U. Kaka ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Adverse Effects ◽  
Meat Quality ◽  
Tissue Damage ◽  
High Frequency ◽  
Sensory Input ◽  
Low Frequency ◽  
Blood Parameters ◽  
Brain Electrical Activity ◽  
High Frequencies

Electrical stunning is the most widely used stunning method for sheep and goats. Because low frequency head-to-back electrical stunning induces cardiac arrest, it is non-compliant with halal requirements. In addition, conventional head-only electrical stunning method can have adverse effects on carcass and meat quality. To address these issues high-frequency electrical stunning systems were developed. High frequency head-to-back removes the potential for pain and distress compared with non-stunned slaughter and does not induce cardiac arrest like low frequency head-to-back stunning making it appropriate for halal. However, this claim is yet to be proven through a comprehensive neurophysiological study. Thus, the present study examined the effects of different pre-slaughter electrical stunning methods and slaughter without stunning on electroencephalographic and blood biochemistry changes. Thirty-two male Boer crossbred bucks were distributed into four groups of eight animals each and subjected to slaughter without stunning (SWS), low frequency head-only (LFHO) and low frequency head-to-back (LFHB) or high frequency head-to-back electrical stunning (HFHB). Slaughtering of animals with or without stunning was performed under minimal anaesthesia. Based on electroencephalograph results, at slaughter, the SWS animals showed an increase in brain electrical activity, which is consistent with the presence of post slaughter noxious sensory input associated with tissue damage. Meanwhile the electroencephalograph activities decreased immediately after application of electrical stunning in all animals, suggesting that they were insensible to pain during and after the neck cut. Electrical brain activities for goats subjected to HFHB were comparable to those for LFHO and those for LFHB. The percentage of animals that exhibited severe clonic activity was significantly lower after LFHB or HFHB stunning compared with LFHO. The concentrations of catecholamines and hyperglycemia in electrically stunned goats were higher compared with those from SWS. It was observed that LFHB caused cardiac arrest in all goats whereas all HFHB goats had no cardiac arrest. High frequency head-to-back electrical stunning may be an improvement on non-stunned slaughter and has advantages for meat quality. However, stunning effectiveness after head-to-back electrical stunning with high frequencies needs to be evaluated under industrial conditions before any recommendation is given.

scholarly journals Model involving gene inactivation in the generation of autosomal recessive mutants in mammalian cells in culture.

1982 ◽  
Vol 2 (9) ◽  
pp. 1126-1133 ◽  
Author(s):  
A E Simon ◽  
M W Taylor ◽  
W E Bradley ◽  
L H Thompson
Keyword(s):  
Chinese Hamster Ovary ◽  
High Frequency ◽  
Mammalian Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Low Frequency ◽  
Two Dimensional ◽  
Wild Type ◽  
Proposed Model ◽  
Class 1

We present evidence for a two-step model for expression of the recessive phenotype at the diploid adenine phosphoribosyl transferase (aprt) locus in Chinese hamster ovary cells. This model proposes a high-frequency event leading to allelic inactivation and a low-frequency event leading to a structural alteration of the APRT protein. Either event can occur first, resulting in two types of heterozygous cells. The proposed model is based on analysis of Chinese hamster ovary presumptive aprt heterozygotes and APRT- mutants, derived by two different laboratories. The major class of heterozygotes (class 1) had approximately 50% parental APRT activity, 50% immunologically precipitable APRT protein, and only wild-type enzyme as based on two-dimensional gel electrophoresis and thermal inactivation studies. We propose that one allele at the aprt locus has been inactivated in these heterozygotes. APRT- mutants derived from any single class 1 heterozygote arose at a low frequency and contained either no immunologically detectable APRT protein or an APRT enzyme which was, in most cases, demonstrably altered. The second class of heterozygotes, consisting of two independent isolates, gave rise to APRT- cells at a high frequency (10(-3) to 10(-5). These heterozygous cell lines had 50% of parental APRT activity and only wild-type spot, or wild-type and an electrophoretic variant spot, on two-dimensional gels. These aprt heterozygotes appear to have arisen by mutation at one allele. APRT- mutants derived from either heterozygote of this class had all lost the wild-type activity, consistent with the proposed model.

Permeability and borehole Stoneley waves: Comparison between experiment and theory

Geophysics ◽  
1989 ◽  
Vol 54 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Kenneth W. Winkler ◽  
Hsui‐Lin Liu ◽  
David Linton Johnson
Keyword(s):  
High Frequency ◽  
Low Frequency ◽  
Theoretical Models ◽  
Oil Field ◽  
Stoneley Wave ◽  
Biot Theory ◽  
Frequency Dependent ◽  
Low Frequencies ◽  
High Frequencies ◽  
Theoretical Predictions

We performed laboratory experiments to evaluate theoretical models of borehole. Stoneley wave propagation in permeable materials. A Berea sandstone and synthetic samples made of cemented glass beads were saturated with silicone oils. We measured both velocity and attenuation over a frequency band from 10 kHz to 90 kHz. Our theoretical modeling incorporated Biot theory and Deresiewicz‐Skalak boundary conditions into a cylindrical geometry and included frequency‐dependent permeability. By varying the viscosity of the saturating pore fluid, we were able to study both low‐frequency and high‐frequency regions of Biot theory, as well as the intermediate transition zone. In both low‐frequency and high‐frequency regions of the theory, we obtained excellent agreement between experimental observations and theoretical predictions. Velocity and attenuation (1/Q) are frequency‐dependent, especially at low frequencies. Also at low frequencies, velocity decreases and attenuation increases with increasing fluid mobility (permeability/viscosity). More complicated behavior is observed at high frequencies. These results support recent observations from the oil field suggesting that Stoneley wave velocity and attenuation may be indicative of formation permeability.

Nonlinear Dynamic Behaviour of the Intervertebral Disc

2013 ◽  
Author(s):  
Giacomo Marini ◽  
Gerd Huber ◽  
Stephen J. Ferguson
Keyword(s):  
Dynamic Response ◽  
Intervertebral Disc ◽  
High Frequency ◽  
Nonlinear Dynamic ◽  
Mechanical Response ◽  
Numerical Models ◽  
Low Frequency ◽  
Upper Body ◽  
Highly Nonlinear ◽  
Flexion Extension

The intervertebral disc, like many collagen-based tissues, has a mechanical response which is highly nonlinear (1). This characteristic is due to both the arrangement and composition of the tissue constituents of the disc (2). Over the past decades several studies have reported the nonlinear response of the disc for different loading scenarios. In particular, past studies were focused on the quasi-static and low frequency (< 10Hz) response to pure and combined cyclic loading, such as axial compression, shear, flexion/extension moment (3–6). The information provided by these studies has been applied in several fields, from the validation of numerical models to the development of disc prostheses. However, such loading conditions are only partially representative of the in-situ load that the intervertebral disc normally experiences. High frequency dynamics stimuli, such as that experienced while driving a car on a rough surface or driving heavy industrial machinery, are also important. It is well known that long-term exposure to vibrational loading is detrimental to normal disc metabolism (7,8). Despite its relevance only a few studies have investigated the dynamic response of the disc to high frequency vibration (9,10) with sometimes different outcomes. In particular, no study has shown an asymmetric, nonlinear dynamic behavior of the system, even though it is evident in quasi-static testing — the well-known tension / compression asymmetry. This aspect is somehow neglected when building rigid body models of the upper body for impact simulation where a Kelvin-Voigt model with linear stiffness is normally used. The aim of this experimental study was therefore to investigate the nonlinear dynamic response of the intervertebral disc to high frequency loadings, taking different pre-loads and displacement amplitude into account.

Mechanical Response of the Lungs at High Frequencies

1978 ◽  
Vol 100 (2) ◽  
pp. 57-66 ◽  
Author(s):  
J. J. Fredberg ◽  
A. Hoenig
Keyword(s):  
Efficient Method ◽  
High Frequency ◽  
Input Impedance ◽  
Mechanical Response ◽  
Airway Wall ◽  
Frequency Range ◽  
Resonant Frequencies ◽  
High Frequencies ◽  
Branching Networks ◽  
Adult Humans

We put forward an efficient method for computing the input impedance of complex asymmetrically branching duct networks, and apply this method to simulation of the dynamic response of the lungs of normal adult humans in the frequency range extending to 10,000 Hz. The results indicate that the response of comparable symmetric and asymmetric branching networks differ at high frequency (> 2 kHz in air), and that the airway wall response is an important factor in determining system damping and resonant frequencies.

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