scholarly journals Functional metagenomic analysis of quorum sensing signaling in a nitrifying community

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chuan Hao Tan ◽  
Yee Phan Yeo ◽  
Muhammad Hafiz ◽  
Noele Kai Jing Ng ◽  
Sujatha Subramoni ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Quorum Sensing ◽  
Activated Sludge ◽  
Treatment Plant ◽  
Feedback System ◽  
Homoserine Lactone ◽  
Community Interactions ◽  
Gene Encoding ◽  
Secondary Messenger

AbstractQuorum sensing (QS) can function to shape the microbial community interactions, composition, and function. In wastewater treatment systems, acylated homoserine lactone (AHL)-based QS has been correlated with the conversion of floccular biomass into microbial granules, as well as EPS production and the nitrogen removal process. However, the role of QS in such complex communities is still not fully understood, including the QS-proficient taxa and the functional QS genes involved. To address these questions, we performed a metagenomic screen for AHL genes in an activated sludge microbial community from the Ulu Pandan wastewater treatment plant (WWTP) in Singapore followed by functional validation of luxI activity using AHL biosensors and LC–MSMS profiling. We identified 13 luxI and 30 luxR homologs from the activated sludge metagenome. Of those genes, two represented a cognate pair of luxIR genes belonging to a Nitrospira spp. and those genes were demonstrated to be functionally active. The LuxI homolog synthesized AHLs that were consistent with the dominant AHLs in the activated sludge system. Furthermore, the LuxR homolog was shown to bind to and induce expression of the luxI promoter, suggesting this represents an autoinduction feedback system, characteristic of QS circuits. Additionally, a second, active promoter was upstream of a gene encoding a protein with a GGDEF/EAL domain, commonly associated with modulating the intracellular concentration of the secondary messenger, c-di-GMP. Thus, the metagenomic approach used here was demonstrated to effectively identify functional QS genes and suggests that Nitrospira spp. maybe QS is active in the activated sludge community.

scholarly journals Activated Sludge Microbial Community and Treatment Performance of Wastewater Treatment Plants in Industrial and Municipal Zones

2020 ◽  
Vol 17 (2) ◽  
pp. 436 ◽  
Author(s):  
Yongkui Yang ◽  
Longfei Wang ◽  
Feng Xiang ◽  
Lin Zhao ◽  
Zhi Qiao
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Wastewater Sludge ◽  
Community Diversity ◽  
Nitrogen And Phosphorus ◽  
Metabolic Functions

Controlling wastewater pollution from centralized industrial zones is important for reducing overall water pollution. Microbial community structure and diversity can adversely affect wastewater treatment plant (WWTP) performance and stability. Therefore, we studied microbial structure, diversity, and metabolic functions in WWTPs that treat industrial or municipal wastewater. Sludge microbial community diversity and richness were the lowest for the industrial WWTPs, indicating that industrial influents inhibited bacterial growth. The sludge of industrial WWTP had low Nitrospira populations, indicating that influent composition affected nitrification and denitrification. The sludge of industrial WWTPs had high metabolic functions associated with xenobiotic and amino acid metabolism. Furthermore, bacterial richness was positively correlated with conventional pollutants (e.g., carbon, nitrogen, and phosphorus), but negatively correlated with total dissolved solids. This study was expected to provide a more comprehensive understanding of activated sludge microbial communities in full-scale industrial and municipal WWTPs.

Isolation of Biofilm-Forming Bacteria from the Secondary Effluent of the Wastewater Treatment Plant and its Ability to Produce N-Acylhomoserine Lactone as Quorum Sensing Signal

2017 ◽  
Vol 863 ◽  
pp. 135-140 ◽  
Author(s):  
Eri Nasuno ◽  
Yuto Abe ◽  
Ken-ichi Iimura ◽  
Masaki Ohno ◽  
Tetsuji Okuda ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Quorum Sensing ◽  
Wastewater Treatment Plant ◽  
Biofilm Formation ◽  
Communication Systems ◽  
Treatment Plant ◽  
Homoserine Lactone ◽  
Side Chain ◽  
Secondary Effluent ◽  
Acyl Side Chain

Bacterial quorum sensing (QS) is one of the cell-to-cell communication systems. N-Acyl-homoserine lactones (AHLs) are the most common QS signals and responsible for biofilm formation in gram-negative bacteria. Inactivation of QS, also referred to as quorum quenching, has been regarded as a popular strategy to control membrane bioreactor (MBR) operations because biofouling in MBR wastewater treatment systems is often caused by biofilm formation. In the present study, 24 bacterial strains were isolated from the secondary effluent of a wastewater treatment plant and AHL productivity and biofilm formation were evaluated on 8 out of 24 isolates. The isolated 8 strains can produce AHLs that only possess a long (C8-C14) acyl side chain. The representative 3 strains were selected from these isolates based on the higher activity of biofilm formation. The AHL separation analysis with a thin layer chromatography clearly showed that N-octanoyl-L-homoserine lactone (C8-HSL) and N-hexanoyl-L-homoserine lactone (C6-HSL) existed in the secondary effluent sample, while no C6-HSL producing strain was isolated. C8-HSL was identified as the product of isolate No. 6. These results suggest that the secondary effluent probably contains various cell-to-cell signaling molecules derived not only from the inhabitants but also from the other microorganisms involved in the activated sludge for the biological pre-treatment. In this secondary effluent, AHL trapping techniques can be proposed as one of the acceptable strategies for the control of the QS systems because the remaining AHLs have relatively long acyl side chain and low concentrations.

The Effect of Copper on The Structure of the Ammonia-Oxidizing Microbial Community in an Activated Sludge Wastewater Treatment Plant

2008 ◽  
Vol 57 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Pamela Principi ◽  
Federica Villa ◽  
Barbara Giussani ◽  
Elisabetta Zanardini ◽  
Francesca Cappitelli ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Effect Of Copper

DESIGN PLANNING WASTEWATER TREATMENT PLANT OF NATA DE COCO INDUSTRY WITH THE ACTIVATED SLUDGE PROCESS

2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Dinda Rita K. Hartaja ◽  
Imam Setiadi
Keyword(s):  
Waste Water ◽  
Wastewater Treatment ◽  
Activated Sludge ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Appropriate Technology ◽  
Waste Water Treatment Plant ◽  
Activated Sludge Process ◽  
High Level

Generally, wastewater of nata de coco industry contains suspended solids and COD were high, ranging from 90,000 mg / l. The high level of of the wastewater pollutants, resulting in nata de coco industry can not be directly disposed of its wastewater into the environment agency. Appropriate technology required in order to process the waste water so that the treated water can meet the environmental quality standards that are allowed. Designing the waste water treatment plant that is suitable and efficient for treating industrial wastewater nata de coco is the activated sludge process. Wastewater treatment using activated sludge process of conventional (standard) generally consists of initial sedimentation, aeration and final sedimentation.Keywords : Activated Sludge, Design, IPAL

Rehabilitation and Upgrading of the Beni Suef City Wastewater Treatment Plant

1990 ◽  
Vol 22 (7-8) ◽  
pp. 131-138
Author(s):  
Ahmed Fadel
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Primary Treatment ◽  
High Rate ◽  
Shock Load ◽  
Economic Evaluations ◽  
Activated Sludge Process ◽  
Trickling Filter

Many of Egypt's cities have existing treatment plants under operation that have been constructed before 1970. Almost all of these treatment plants now need rehabilitation and upgrading to extend their services for a longer period. One of these plants is the Beni Suef City Wastewater Treatment Plant. The Beni Suef WWTP was constructed in 1956. It has primary treatment followed by secondary treatment employing intermediate rate trickling filters. The BOD, COD, and SS concentration levels are relatively high. They are approximately 800, 1100, and 600 mg/litre, respectively. The Beni Suef city required the determination of the level of work needed for the rehabilitation and upgrading of the existing 200 l/s plant and to extend its capacity to 440 l/s at year 2000 A description of the existing units, their deficiencies and operation problems, and the required rehabilitation are presented and discussed in this paper. Major problems facing the upgrading were the lack of space for expansion and the shortage of funds. It was, therefore, necessary to study several alternative solutions and methods of treatment. The choice of alternatives was from one of the following schemes: a) changing the filter medium, its mode of operation and increasing the number of units, b) changing the trickling filter to high rate and combining it with the activated sludge process, for operation by one of several possible combinations such as: trickling filter-solids contact, roughing filter-activated sludge, and trickling filter-activated sludge process, c) dividing the flow into two parts, the first part to be treated using the existing system and the second part to be treated by activated sludge process, and d) expanding the existing system by increasing the numbers of the different process units. The selection of the alternative was based on technical, operational and economic evaluations. The different alternatives were compared on the basis of system costs, shock load handling, treatment plant operation and predicted effluent quality. The flow schemes for the alternatives are presented. The methodology of selecting the best alternative is discussed. From the study it was concluded that the first alternative is the most reliable from the point of view of costs, handling shock load, and operation.

Operational Results of the Wolfsburg Wastewater Treatment Plant

1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Phosphate Removal ◽  
Total Nitrogen Content ◽  
Activated Sludge Process

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.

Management of toxicity effects in a large wastewater treatment plant

1997 ◽  
Vol 36 (2-3) ◽  
pp. 1-8 ◽  
Author(s):  
P. Grau ◽  
B. P. Da-Rin
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Severe Case ◽  
Treatment Efficiency ◽  
Filamentous Bulking ◽  
Biological Phenomena ◽  
Inhibition Of Nitrification ◽  
Toxicity Effects

An unusually severe case of toxicity accompanied by activated sludge filamentous bulking was observed at the wastewater treatment plant Sao Paulo-Barueri. Treatment efficiency of the plant, operated without major problems for more than five years before, was significantly hindered for almost six months. Occurrence of toxic shocks was confirmed partly directly but mostly indirectly by inhibition of nitrification and biological phenomena related to toxicity. Several measures adopted, including the recycled activated sludge chlorination, are described in the paper.

scholarly journals The diversity of active microbial groups in an activated sludge process treating painting process wastewater

2020 ◽  
Vol 148 ◽  
pp. 01002
Author(s):  
Herto Dwi Ariesyady ◽  
Mentari Rizki Mayanda ◽  
Tsukasa Ito
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Activated Sludge ◽  
Biological Treatment ◽  
Rrna Gene ◽  
Activated Sludge Process ◽  
Painting Process ◽  
Process Wastewater

Activated sludge process is one of the wastewater treatment method that is applied for many wastewater types including painting process wastewater of automotive industry. This wastewater is well-known to have high heavy metals concentration which could deteriorate water environment if appropriate performance of the wastewater treatment could not be achieved. In this study, we monitored microbial community diversity in a Painting Biological Treatment (PBT) system. We applied a combination of cultivation and genotypic biological methods based on 16S rRNA gene sequence analysis to identify the diversity of active microbial community. The results showed that active microbes that could grow in this activated sludge system were dominated by Gram-negative bacteria. Based on 16S rRNA gene sequencing analysis, it was revealed that their microbial diversity has close association with Bacterium strain E286, Isosphaera pallida, Lycinibacillus fusiformis, Microbacterium sp., Orchobactrum sp., Pseudomonas guariconensis, Pseudomonas sp. strain MR84, Pseudomonas sp. MC 54, Serpens sp., Stenotrophomonas acidaminiphila, and Xylella fastidiosa with similarity of 86 – 99%. This findings reflects that microbial community in a Painting Biological Treatment (PBT) system using activated sludge process could adapt with xenobiotics in the wastewater and has a wide range of diversity indicating a complex metabolism mechanism in the treatment process.

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