scholarly journals Publisher Correction: Freeform micropatterning of living cells into cell culture medium using direct inkjet printing

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Ju An Park ◽  
Sejeong Yoon ◽  
Jimin Kwon ◽  
Hesung Now ◽  
Young Kwon Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Inkjet Printing ◽  
Culture Medium ◽  
Living Cells ◽  
Cell Culture Medium

scholarly journals Freeform micropatterning of living cells into cell culture medium using direct inkjet printing

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ju An Park ◽  
Sejeong Yoon ◽  
Jimin Kwon ◽  
Hesung Now ◽  
Young Kwon Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Inkjet Printing ◽  
Culture Medium ◽  
Living Cells ◽  
Cell Culture Medium

scholarly journals Author Correction: Freeform micropatterning of living cells into cell culture medium using direct inkjet printing

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ju An Park ◽  
Sejeong Yoon ◽  
Jimin Kwon ◽  
Hesung Now ◽  
Young Kwon Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Inkjet Printing ◽  
Culture Medium ◽  
Living Cells ◽  
Cell Culture Medium

Experiments in Nanomechanical Properties of Live Osteoblast Cells and Cell–Biomaterial Interface

2011 ◽  
Vol 2 (4) ◽  
Author(s):  
Rohit Khanna ◽  
Kalpana S. Katti ◽  
Dinesh R. Katti
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Structure ◽  
Living Cells ◽  
Actin Stress Fiber ◽  
Cell Culture Medium ◽  
Nanomechanical Properties ◽  
Cell Substrate ◽  
Significant Difference

Characterizing the mechanical characteristics of living cells and cell–biomaterial composite is an important area of research in bone tissue engineering. In this work, an in situ displacement-controlled nanoindentation technique (using Hysitron Triboscope) is developed to perform nanomechanical characterization of living cells (human osteoblasts) and cell–substrate constructs under physiological conditions (cell culture medium; 37 °C). In situ elastic moduli (E) of adsorbed proteins on tissue culture polystyrene (TCPS) under cell culture media were found to be ∼4 GPa as revealed by modulus mapping experiments. The TCPS substrates soaked in cell culture medium showed significant difference in surface nanomechanical properties (up to depths of ∼12 nm) as compared to properties obtained from deeper indentations. Atomic force microscopy (AFM) revealed the cytoskeleton structures such as actin stress fiber networks on flat cells which are believed to impart the structural integrity to cell structure. Load-deformation response of cell was found to be purely elastic in nature, i.e., cell recovers its shape on unloading as indicated by linear loading and unloading curves obtained at 1000 nm indentation depth. The elastic response of cells is obtained during initial cell adhesion (ECell, 1 h, 1000 nm = 4.4–12.4 MPa), cell division (ECell, 2 days, 1000 nm = 1.3–3.0 MPa), and cell spreading (ECell, 2 days, 1000 nm = 6.9–11.6 MPa). Composite nanomechanical responses of cell–TCPS constructs were obtained by indentation at depths of 2000 nm and 3000 nm on cell-seeded TCPS. Elastic properties of cell–substrate composites were mostly dominated by stiff TCPS (EBulk = 5 GPa) lying underneath the cell.

scholarly journals Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Enkhtuul Gantumur ◽  
Masaki Nakahata ◽  
Masaru Kojima ◽  
Shinji Sakai
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Culture Medium ◽  
Living Cells ◽  
Cross Linking ◽  
3D Bioprinting ◽  
Cell Culture Medium ◽  
Adhesive Surface ◽  
Cell Adhesive ◽  
Human Nose

We report an extrusion-based bioprinting approach, in which stabilization of extruded bioink is achieved through horseradish peroxidase (HRP)-catalyzed cross-linking consuming hydrogen peroxide (H2O2) supplied from HRP and glucose. The bioinks containing living cells, HRP, glucose, alginate possessing phenolic hydroxyl (Ph) groups, and cellulose nanofiber were extruded to fabricate 3D hydrogel constructs. Lattice- and human nose-shaped 3D constructs were successfully printed and showed good stability in cell culture medium for over a week. Mouse 10T1/2 fibroblasts enclosed in the printed constructs remained viable after 7 days of culture. It was also able to switch a non-cell-adhesive surface of the printed construct to cell-adhesive surface for culturing cells on it through a subsequent cross-linking of gelatin possessing Ph moieties. These results demonstrate the possibility of utilizing the presented cross-linking method for 3D bioprinting.

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium

scholarly journals Defined MSC exosome with high yield and purity to improve regenerative activity

2021 ◽  
Vol 12 ◽  
pp. 204173142110086
Author(s):  
Jun Yong Kim ◽  
Won-Kyu Rhim ◽  
Yong-In Yoo ◽  
Da-Seul Kim ◽  
Kyoung-Won Ko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Density Lipoprotein ◽  
High Yield ◽  
Human Umbilical Cord ◽  
Cell Culture Medium ◽  
Human Coronary Artery ◽  
Isolation Methods ◽  
Regenerative Activity ◽  
Yield And Purity

Exosomes derived from mesenchymal stem cells (MSCs) have been studied as vital components of regenerative medicine. Typically, various isolation methods of exosomes from cell culture medium have been developed to increase the isolation yield of exosomes. Moreover, the exosome-depletion process of serum has been considered to result in clinically active and highly purified exosomes from the cell culture medium. Our aim was to compare isolation methods, ultracentrifuge (UC)-based conventional method, and tangential flow filtration (TFF) system-based method for separation with high yield, and the bioactivity of the exosome according to the purity of MSC-derived exosome was determined by the ratio of Fetal bovine serum (FBS)-derived exosome to MSC-derived exosome depending on exosome depletion processes of FBS. The TFF-based isolation yield of exosome derived from human umbilical cord MSC (UCMSC) increased two orders (92.5 times) compared to UC-based isolation method. Moreover, by optimizing the process of depleting FBS-derived exosome, the purity of UCMSC-derived exosome, evaluated using the expression level of MSC exosome surface marker (CD73), was about 15.6 times enhanced and the concentration of low-density lipoprotein-cholesterol (LDL-c), known as impurities resulting from FBS, proved to be negligibly detected. The wound healing and angiogenic effects of highly purified UCMSC-derived exosomes were improved about 23.1% and 71.4%, respectively, with human coronary artery endothelial cells (HCAEC). It suggests that the defined MSC exosome with high yield and purity could increase regenerative activity.

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