scholarly journals Antimicrobial Efficacy Assessment of Human Derived Composite Amnion-Chorion Membrane

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nathan D. Palanker ◽  
Chun-Teh Lee ◽  
Robin L. Weltman ◽  
Gena D. Tribble ◽  
Ransome van der Hoeven ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Antimicrobial Properties ◽  
Antimicrobial Efficacy ◽  
Oral Streptococci ◽  
Time Points ◽  
Traditional Assessment ◽  
Efficacy Assessment ◽  
Collagen Membranes ◽  
Porcine Pericardium

Abstract Human derived composite amnion-chorion membrane (ACM) has been used to facilitate wound healing due to reported anti-inflammatory properties and promotion of cell proliferation. This study aimed to assess the antimicrobial properties of the ACM using novel methods to visualize the antimicrobial efficacy of membranes in situ at different time points. Porcine Pericardium Collagen Membranes (PPCM) served as membrane controls. Circular pieces of the membranes were used in three different assays: insert, agar contact and glass-bottom well assays. Streptococcus gordonii were spotted onto the membranes and the plates were subsequently centrifuged to ensure direct bacterial contact with the membranes in the insert and agar contact assays, thus better mimicking bacterial adherence in the oral cavity. After incubation at 37 °C for 8, 24, and 48 hours, the membranes were dyed with the Live/Dead BacLight Bacterial Viability fluorescence stain and analyzed via confocal microscopy. The results demonstrated that the ACM completely inhibited bacterial growth at all time points, whereas the PPCM did not demonstrate any antimicrobial properties. Within the limits of this study, the ACM showed extremely high antimicrobial efficacy against oral streptococci. In addition, our methods may be useful in assessing antimicrobial properties for biomaterials with minimum diffusion ability, when traditional assessment methods are not applicable.

scholarly journals Radiomorphology of the Habib Sealer-Induced Resection Plane during Long-Time Followup:A Longitudinal Single Center Experience after 64 Radiofrequency-AssistedLiver Resections

HPB Surgery ◽  
2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Robert Kleinert ◽  
Roger Wahba ◽  
Christoph Bangard ◽  
Klaus Prenzel ◽  
Arnulf H. Hölscher ◽  
...  
Keyword(s):  
Liver Resection ◽  
Morphological Characteristics ◽  
Postoperative Mortality ◽  
Coagulation Necrosis ◽  
Time Interval ◽  
Safe Alternative ◽  
Time Points ◽  
Single Center Experience ◽  
Long Time

Background. Radiofrequency (RF-) assisted liver resection devices like the Habib sealer induce a necrotic resection plane from which a small margin of necrotic liver tissue remains in situ. The aim of the present paper was to report our long-time experience with the new resection method and the morphological characteristics of the remaining necrotic resection plane. Methods. 64 RF-assisted liver resections were performed using the Habib sealer. Followup was assessed at defined time points. Results. The postoperative mortality was 3,6% and morbidity was 18%. The followup revealed that the necrotic zone was detectable in all analyzed CT and MRI images as a hypodense structure without any contrast enhancement at all time points, irrespectively of the time interval between resection and examination. Conclusion. Liver resection utilizing radiofrequency-induced resection plane coagulation is a safe alternative to the established resection techniques. The residual zone of coagulation necrosis remains basically unchanged during a followup of three years. This has to be kept in mind when evaluating the follow up imaging of these patients.

Noninvasive Carcinoma of the Breast: Angiogenesis and Cell Proliferation

2004 ◽  
Vol 128 (8) ◽  
pp. 893-896 ◽  
Author(s):  
Ying Cao ◽  
Gladell P. Paner ◽  
Leonard B. Kahn ◽  
Prabha B. Rajan
Keyword(s):  
Cell Proliferation ◽  
Microvessel Density ◽  
Carcinoma In Situ ◽  
Nuclear Grade ◽  
Proliferation Index ◽  
Carcinoma Of The Breast ◽  
Cell Proliferation Index ◽  
Noninvasive Carcinoma ◽  
Mib 1

Abstract Context.—Angiogenesis and the cell proliferation index can predict the prognosis of invasive breast carcinoma; however, little is known of their roles in noninvasive tumor. Objective.—To investigate the correlation of microvessel density and cell proliferation index with other histologic parameters (histologic type, nuclear grade, and mitotic count) in 65 cases of noninvasive carcinoma of the breast. Design.—Formalin-fixed, paraffin-embedded tissues from 65 cases of carcinoma in situ of the breast were immunostained with antibody against factor VIII antigen and proliferation-associated nuclear antigen MIB-1. The microvessel density was measured by counting the total number of microvessels around the carcinoma in situ per 10 low-power microscopic fields. The cell proliferation index was calculated by counting MIB-1–positive nuclei in 100 tumor cells. A χ2 test and Spearman rank correlation test were used for statistical analysis. Results.—The microvessel density and cell proliferation index of comedo-type, high-nuclear-grade ductal carcinomas in situ are significantly higher than those of either noncomedo type ductal carcinomas in situ or lobular carcinoma in situ (P < .001). Conclusions.—Angiogenesis and the cell proliferation index are active biological processes and may be considered as markers to separate low- and high-risk patients with noninvasive breast carcinomas.

Contrasting patterns of c-myc and N-myc expression in proliferating, quiescent, and differentiating cells of the embryonic chicken lens

Development ◽  
1992 ◽  
Vol 115 (3) ◽  
pp. 813-820
Author(s):  
L.L. Harris ◽  
J.C. Talian ◽  
P.S. Zelenka
Keyword(s):  
Cell Proliferation ◽  
Protein Product ◽  
Mrna Levels ◽  
Lens Fiber ◽  
Proliferating Cells ◽  
Fiber Cells ◽  
Embryonic Chicken ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

The present study uses the polymerase chain reaction and in situ hybridization to examine c-myc and N-myc mRNA in the embryonic chicken lens at 6, 10, 14 and 19 days of development and compares the pattern of expression obtained with the developmental pattern of cell proliferation and differentiation. In the central epithelium, c-myc mRNA levels were proportional to the percentage of proliferating cells throughout development. N-myc mRNA expression in this region was relatively low and showed no correlation with cell proliferation. The ratio of N-myc to c-myc mRNA increased markedly with the onset of epithelial cell elongation and terminal fiber cell differentiation, although both c-myc and N-myc mRNAs continued to be expressed in postmitotic, elongating cells of the equatorial epithelium and in terminally differentiating lens fiber cells. Thus, increased expression of N-myc, a gene whose protein product may compete with c-myc protein for dimerization partners, accompanies the dissociation of c-myc expression and cell proliferation during terminal differentiation of lens fiber cells.

In situ deposition of nano Cu2O on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties

2021 ◽  
Vol 191 ◽  
pp. 600-607
Author(s):  
Xinglu Zhou ◽  
Anlin Yin ◽  
Junlu Sheng ◽  
Jiayan Wang ◽  
Huifen Chen ◽  
...  
Keyword(s):  
Antimicrobial Properties ◽  
Nanofibrous Scaffolds ◽  
In Situ Deposition

scholarly journals Cognate antigen engagement on parenchymal cells stimulates CD8+ T cell proliferation in situ

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Robyn M. Sutherland ◽  
Sarah L. Londrigan ◽  
Jamie L. Brady ◽  
Emma M. Carrington ◽  
Julia M. Marchingo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Cd8 T Cell ◽  
T Cell Proliferation ◽  
Cognate Antigen ◽  
Parenchymal Cells

Cell Proliferation in the Endolymphatic Sac In Situ After the Rat Waldeyer Ring Equivalent Immunostimulation

2003 ◽  
Vol 113 (9) ◽  
pp. 1609-1614 ◽  
Author(s):  
Zhen Yan ◽  
Ji-Bao Wang ◽  
Shu-Sheng Gong ◽  
Xiang Huang
Keyword(s):  
Cell Proliferation ◽  
Endolymphatic Sac

scholarly journals Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

2017 ◽  
Vol 67 (1) ◽  
pp. 1-10
Author(s):  
Gordana Joksić ◽  
Mileva Mićić ◽  
Jelena Filipović ◽  
Dunja Drakulić ◽  
Miloš Stanojlović ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Immunohistochemical Analysis ◽  
Optimal Dose ◽  
Cell Labeling ◽  
Assay Method ◽  
Proliferating Cells ◽  
Adult Rats ◽  
In Vivo Labeling

AbstractThe study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.

The relationship between cell proliferation and the transcription of the nuclear oncogenes c-myc, c-myb and c-ets-1 during feather morphogenesis in the chick embryo

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 699-713 ◽  
Author(s):  
X. Desbiens ◽  
C. Queva ◽  
T. Jaffredo ◽  
D. Stehelin ◽  
B. Vandenbunder
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Spatial Distribution ◽  
Chick Embryo ◽  
Expression Patterns ◽  
S Phase ◽  
Skin Morphogenesis ◽  
Dermal Cells ◽  
The Relationship

We have described the expression of three nuclear protooncogenes, c-myc, c-myb and c-ets-1 during feather morphogenesis in the chick embryo. In parallel with the expression patterns obtained by in situ hybridization, we have mapped the spatial distribution of S-phase cells by monitoring the incorporation of 5-bromodeoxyuridine. We do not detect c-myc or c-myb transcripts during the early stages when S-phase cells are scattered in the dermis and in the epidermis. Rather c-ets-1 transcripts are abundant in the dermal cells which divide and accumulate under the uniform epidermis. At the onset of the formation of the feather bud, cells within each rudiment cease DNA replicative activities and c-myc transcripts are detected both in the epidermis and in the underlying dermis. This expression precedes the reentry into the S phase. The transcription of c-myb, which has been previously tightly linked to hemopoietic cells is also detected in the developing skin. This expression is essentially located in proliferating epidermal cells on and after the beginning of feather outgrowth. As feather outgrowth proceeds, the distribution of c-myc and c-myb transcripts is restricted to the highly proliferating epidermis. In contrast c-ets-1 transcripts are never detected in the epidermis. During the later stages of skin morphogenesis, the transcription of c-ets-1 is restricted to the endothelial cells of blood vessels, as previously described. We suggest that the differential expression of these nuclear oncogenes reflects the activation of different mitotic controlling pathways during the development of the skin.

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