EVALUATION OF WASTE MUSHROOM MEDIUM AS A FERMENTABLE SUBSTRATE AND BIOETHANOL PRODUCTION

2012 ◽  
Vol 06 ◽  
pp. 745-750
Author(s):  
AI ASAKAWA ◽  
CHIZURU SASAKI ◽  
CHIKAKO ASADA ◽  
YOSHITOSHI NAKAMURA
Keyword(s):  
Steam Explosion ◽  
Lentinula Edodes ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Steam Pressure ◽  
Insoluble Residue ◽  
Steam Explosion Pretreatment ◽  
Simultaneous Saccharification ◽  
Waste Mushroom Medium

Waste Shiitake (Lentinula edodes) mushroom medium, a lignocellulosic aglicultural residue, was evaluated as a fermentable substrate. 87% of the fermentable sugars remained in the waste mushroom medium. The sugar yield of the waste mushroom medium (46.3%) was higher than that of raw mushroom medium (20.3%) after 48 h of enzymatic saccharification by Meicelase because L. edodes changed wood structure. These results indicated that the waste mushroom medium is a suitable substrate for fermentation. Next, the efficient ethanol production using steam explosion pretreatment was studied. After 30 h of simultaneous saccharification and fermentation (SSF) using Meicelase and Saccharomyces cerevisiae AM12, 20.0 g/L ethanol was produced from 100 g/L water-insoluble residue of the waste mushroom medium treated at a steam pressure of 20 atm and a steaming time of 5 min. This corresponded to an ethanol yield of 77.0% of the theoretical, i.e. 14.7 g of ethanol obtained from 100 g of waste mushroom medium.

scholarly journals Pretreatment of Corn Stover Using an Extremely Low-Liquid Ammonia (ELLA) Method for the Effective Utilization of Sugars in Simultaneous Saccharification and Fermentation (SSF) of Ethanol

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 191
Author(s):  
Tin Diep Trung Le ◽  
Vi Phuong Nguyen Truong ◽  
My Thi Tra Ngo ◽  
Tae Hyun Kim ◽  
Kyeong Keun Oh
Keyword(s):  
Corn Stover ◽  
Liquid Ammonia ◽  
Elevated Temperatures ◽  
Simultaneous Saccharification And Fermentation ◽  
Aqueous Ammonia ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Extended Period ◽  
Pretreatment Conditions ◽  
Simultaneous Saccharification

Extremely low-liquid ammonia (ELLA) pretreatment using aqueous ammonia was investigated in order to enhance the enzymatic saccharification of corn stover and subsequent ethanol production. In this study, corn stover was treated with an aqueous ammonia solution at different ammonia loading rates (0.1, 0.2, and 0.3 g NH3/g biomass) and various liquid-to-solid (L/S) ratios (0.55, 1.12, and 2.5). The ELLA pretreatment was conducted at elevated temperatures (90–150 °C) for an extended period (24–120 h). Thereafter, the pretreated material was saccharified by enzyme digestion and subjected to simultaneous saccharification and fermentation (SSF) tests. The effects of key parameters on both glucan digestibility and xylan digestibility were analyzed using analysis of variance (ANOVA). Under optimal pretreatment conditions (L/S = 2.5, 0.1 g-NH3/g-biomass, 150 °C), 81.2% glucan digestibility and 61.1% xylan digestibility were achieved. The highest ethanol yield achieved on the SSF tests was 85.4%. The ethanol concentration was 14.5 g/L at 96 h (pretreatment conditions: liquid-to-solid ratio (L/S) = 2.5, 0.1 g-NH3/g-biomass, 150 °C, 24 h. SSF conditions: microorganism Saccharomyces cerevisiae (D5A), 15 FPU/g-glucan, CTech2, 3% w/v glucan, 37 °C, 150 rpm).

scholarly journals Bioethanol Production From Pineapple Wastes

2014 ◽  
Vol 3 (4) ◽  
pp. 60 ◽  
Author(s):  
Alessia Tropea ◽  
David Wilson ◽  
Loredana G. La Torre ◽  
Rosario B. Lo Curto ◽  
Peter Saugman ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Plant Cell Walls ◽  
Theoretical Yield ◽  
Cellulosic Sugars ◽  
Separate Hydrolysis And Fermentation ◽  
Simultaneous Saccharification

<p>There is great interest in producing bioethanol from biomass and there is much emphasis on exploiting lignocellulose sources, from crop wastes through to energy-rich crops. Some waste streams, however, contain both cellulosic and non-cellulosic sugars. These include wastes from pineapple processing.</p> <p>Pineapple wastes are produced in large amounts throughout the world by canning industries. These wastes are rich in intracellular sugars and plant cell walls which are composed mainly of cellulose, pectic substances and hemicelluloses. The purpose of this study was to investigate the potential to transform such residues into ethanol after enzymatic saccharification of plant cell walls, and fermentation of the resulting simple sugars using the <em>Saccharomyces cerevisiae</em> NCYC 2826 strain. Three different fermentation modes, direct fermentation, separate hydrolysis and fermentation, and simultaneous saccharification and fermentation of the biomass were tested and compared. The results show that the main sugars obtained from pineapple waste were: glucose, uronic acid, xylose, galactose, arabinose and mannose. The highest ethanol yield was achieved after 30 hours of simultaneous saccharification and fermentation, and reached up to 3.9% (v/v), corresponding to the 96% of the theoretical yield.</p>

scholarly journals Effect of Pre-hydrolysis on Simultaneous Saccharification and Fermentation of Native Rye Starch

2020 ◽  
Vol 13 (6) ◽  
pp. 923-936 ◽  
Author(s):  
Ewelina Strąk-Graczyk ◽  
Maria Balcerek
Keyword(s):  
Dry Matter ◽  
Agricultural Sector ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Matter Content ◽  
Raw Material ◽  
Process Efficiency ◽  
Dry Matter Content ◽  
Sem Images ◽  
Simultaneous Saccharification

Abstract The rising population and increasing demand for food place added pressure on the agricultural sector to maintain high process efficiency while implementing environmentally friendly methods. In this study, we investigate the effect of pre-hydrolysis of native rye starch and its influence on the yield of ethanol obtained by simultaneous saccharification and fermentation (SSF) from high gravity rye mashes with 25% and 28% w w−1 dry matter content. Fermentation was carried out in a 3-day system at a temperature of 35 ± 1 °C using the dry distillery yeast Ethanol Red (Saccharomyces cerevisiae). The characteristics of the tested raw material and changes in the native rye starch during enzymatic hydrolysis were analyzed using a scanning electron microscope (SEM). The SEM images revealed characteristic changes on the surface of the starch, which was found to have a layered structure, as well as interesting behavior by the yeast during SSF when the glucose concentration in the environment was lowered. Both in the mashes with 25% and 28% w w−1 dry matter, starch pre-hydrolysis did not significantly increase either the initial amounts of sugars available to the yeast or the fermentation efficiency and ethanol yield in comparison to the mashes without this pre-treatment.

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

scholarly journals Production of ethanol from Jerusalem artichoke by mycelial pellets

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chao Zhang ◽  
Daoji Wu ◽  
Hongqi Yang ◽  
Huixue Ren
Keyword(s):  
Lower Cost ◽  
Simultaneous Saccharification And Fermentation ◽  
Volume Fraction ◽  
Ethanol Yield ◽  
New Technology ◽  
Jerusalem Artichoke ◽  
Mycelial Pellets ◽  
Repeated Use ◽  
Easy Operation ◽  
Simultaneous Saccharification

AbstractMycelial pellets formed by Aspergillus niger A-15 were used to immobilize the ethanol producing yeast Saccharomyces cerevisiae C-15. The operation parameters, such as agitation speed, temperature and mixed proportion of strains were studied. The optimal adsorption 66.9% was obtained when speed was 80r/min, temperature was 40 °C and mixed proportion(mycelial pellets: yeasts) was 1:10. With Jerusalem artichoke flour as substrate, 12.8% (V/V) of ethanol was obtained after 48 h by simultaneous saccharification and fermentation using mycelial pellets. And mycelial pellets could tolerate 19% (volume fraction) ethanol. The above results proved that this new technology was feasible, and it had the advantages of higher ethanol yield, long service life, repeated use, easy operation and lower cost in producing ethanol.

PRODUCTION OF BIOFUEL FROM WASTE LIGNOCELLULOSIC BIOMASS MATERIALS BASED ON ENERGY SAVING VIEWPOINT

2012 ◽  
Vol 06 ◽  
pp. 715-720
Author(s):  
Maki Takano ◽  
Kazuhiro Hoshino
Keyword(s):  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Steam Explosion ◽  
Simultaneous Saccharification And Fermentation ◽  
Biofuel Production ◽  
Fermentable Sugars ◽  
Renewable Material ◽  
Suitable Combination ◽  
Biomass Materials ◽  
Simultaneous Saccharification

To develop biofuel production from waste lignocellulosic biomass materials the rice straw was selected one of renewable material and the degradation condition about pretreatment and enzymatic hydrolysis to obtain effectively fermentable sugars was investigated. Rice straw was pretreated by five kinds of methods and then the components ratio of rice straw was examined. First, the steam explosion was selected based on the degradability and the requirement energy. In addition, the best suitable combination of two cellulases to effective and economical hydrolyze was determined from the degradability of these pretreated rice straws. In the simultaneous saccharification and fermentation of the steam explosion rice straw by combining cellulase cocktail and a novel fermenting fungus, 13.2 g/L ethanol was able to product for 96 h.

Comparison of ultrasound-assisted Fenton reaction and dilute acid-catalysed steam explosion pretreatment of corncobs: cellulose characteristics and enzymatic saccharification

RSC Advances ◽  
2016 ◽  
Vol 6 (80) ◽  
pp. 76848-76854 ◽  
Author(s):  
Sujun Wang ◽  
Xianhong Ouyang ◽  
Wenya Wang ◽  
Qipeng Yuan ◽  
Aixia Yan
Keyword(s):  
Steam Explosion ◽  
Fenton Reaction ◽  
Enzymatic Saccharification ◽  
Dilute Acid ◽  
Steam Explosion Pretreatment ◽  
Ultrasound Assisted

As an emerging method for lignocellulose pretreatment, the ultrasound-assisted Fenton reaction is not well developed in comparison to the dilute acid-catalysed steam explosion.

The influence of the explosive decompression in steam-explosion pretreatment on the enzymatic digestibility of different biomasses

2017 ◽  
Vol 202 ◽  
pp. 269-280 ◽  
Author(s):  
Christoph-M. Seidel ◽  
Thomas Pielhop ◽  
Michael H. Studer ◽  
Philipp Rudolf von Rohr
Keyword(s):  
Corn Stover ◽  
Lignocellulosic Biomass ◽  
Steam Explosion ◽  
Steam Pressure ◽  
Positive Influence ◽  
Herbaceous Plants ◽  
Enzymatic Digestibility ◽  
Maximum Enhancement ◽  
Steam Explosion Pretreatment ◽  
Pretreatment Conditions

For the production of second generation biofuels from lignocellulosic biomass, pretreatment of the biomass feedstock is necessary to overcome its recalcitrance in order to gain fermentable sugars. Due to many reasons, steam-explosion pretreatment is currently the most commonly used pretreatment method for lignocellulosic biomass on a commercial scale [S. Brethauer and M. H. Studer, CHIMIA, 2015, 69, 572–581]. In contrast to others, we showed that the explosive decompression at the end of this pretreatment step can have a positive influence on the enzymatic digestibility of softwood, especially in combination with high enzyme dosages [T. Pielhop, et al., Biotechnology for Biofuels, 2016, 9, 152]. In this study, the influence of the explosive decompression on the enzymatic digestibility of hardwood and herbaceous plants was systematically studied. Beech and corn stover were pretreated under different pretreatment conditions and enzymatically hydrolysed with different enzyme dosages. The maximum enhancement of the digestibility of corn stover was 16.53% after a 2.5 min pretreatment step at 15 barg steam pressure. For beech, a maximum relative enhancement of 58.29% after a 10 min pretreatment step at 15 barg steam pressure could be reached. With this, we show that the explosive decompression can also enhance the enzymatic cellulose digestibility of hardwood and herbaceous plants.

scholarly journals Production of Bioethanol from Selected Lignocellulosic Agrowastes

2019 ◽  
pp. 1-17
Author(s):  
Olotu Emmanuel Juwon ◽  
Olukunle Folake Oluwatoyin
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Titratable Acidity ◽  
Reducing Sugar ◽  
Corn Cobs ◽  
Orange Peels ◽  
Total Titratable Acidity ◽  
Cassava Peels ◽  
Bioethanol Yield ◽  
Simultaneous Saccharification

This study evaluated the ability of cassava peels, banana peels, orange peels and corn cobs hydrolysates to produce bioethanol. Fibre fractions analysis was carried out using standard methods. The samples were pre-treated with acid and base, followed by simultaneous saccharification and fermentation (SSF) for bioethanol production. During fermentation, pH, total titratable acidity, reducing sugar, microbial load and bioethanol yield were determined. The reducing sugar yield for Aspergillus niger and Bacillus cereus were 30.28 g and 13.35 g for corn cobs. The pH was observed to decrease during fermentation period with orange peels having the lowest pH of 2.6 after 240 hours of fermentation using A. Niger and S. cerevisiae, when B. cereus and S. Cerevisiae were used the pH was observed to be 4.10.  Total titratable acidity showed increase in all the substrates, with corn cobs having the highest when B. cereus and S. cerevisiae were used (1.62), followed by cassava peels when A. niger and S. cerevisiae were used (1.52). Highest ethanol yield following simultaneous saccharification and fermentation with A. niger and S. cerevisiae was obtained in corn cobs with 17.43 g/100 g, while orange peels gave the lowest with 8.02 g/100 g, the ethanol yield from each substrates as well as the combined substrates were significantly different at p≤ 0.05. The combined substrates (1:1:1:1) gave the highest ethanol yield of 12.44 g/100 g using A. niger and S. cerevisiae.  This study therefore revealed that A. niger had the highest bioethanol yield using corn cobs as the carbon source, therefore it could be used for mass bioethanol production.

Sign in / Sign up

Export Citation Format

Share Document