scholarly journals Development of a tunable method to generate various three-dimensional microstructures by replenishing macromolecules such as extracellular matrix components and polysaccharides

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fumiya Tao ◽  
Kanae Sayo ◽  
Kazuyuki Sugimoto ◽  
Shigehisa Aoki ◽  
Nobuhiko Kojima
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities.

1985 ◽  
Vol 101 (3) ◽  
pp. 914-923 ◽  
Author(s):  
J Landry ◽  
D Bernier ◽  
C Ouellet ◽  
R Goyette ◽  
N Marceau
Keyword(s):  
Extracellular Matrix ◽  
Rat Liver ◽  
Single Cells ◽  
Three Dimensional ◽  
Cell Types ◽  
Tyrosine Aminotransferase ◽  
Liver Cells ◽  
Specific Pattern ◽  
Extracellular Matrix Components ◽  
Matrix Components

Liver cells isolated from newborn rats and seeded on a non-adherent plastic substratum were found to spontaneously re-aggregate and to form, within a few days, spheroidal aggregates that eventually reached a plateaued diameter of 150-175 micron. Analyses on frozen sections from these spheroids by immunofluorescence microscopy using antibodies to various cytoskeletal elements and extracellular matrix components revealed a sorting out and a histotypic reorganization of three major cell types. A first type consisted of cells that segregated out on the aggregate surface forming a monolayer cell lining; a second type was identified as hepatocytes that regrouped in small islands often defining a central lumen; and a third group of cells reorganized into bile duct-like structures. This intercellular organization in the aggregates was paralleled by the accumulation of extracellular matrix components (laminin, fibronectin, and collagen) and their deposition following a specific pattern around each cell population structure. Determinations of albumin secretion and tyrosine aminotransferase induction by dexamethasone and glucagon at various times after the initiation of the cultures revealed a maintenance of the hepatocyte-differentiated functions for at least up to 2 mo at the levels measured at 3-5 d. It is concluded that cells dispersed as single cells from newborn rat liver conserve in part the necessary information to reconstruct a proper three-dimensional cyto-architecture and that the microenvironment so generated most likely represents a basic requirement for the optimal functioning of these differentiated cells.

scholarly journals Can We Build Artificial Stem Cell Compartments?

2003 ◽  
Vol 2003 (3) ◽  
pp. 164-169 ◽  
Author(s):  
Carlos E. Semino
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Three Dimensional ◽  
Soluble Factors ◽  
Cell Compartments ◽  
Synthetic Materials ◽  
Extracellular Matrix Components ◽  
Main Factors ◽  
Matrix Components

Animals carry stem cells throughout their entire life, from embryogenesis to senescence. Their function during development and adulthood consists basically of forming and sustaining functional tissues while maintaining a small self-renewing population. They reside in a complex three-dimensional environment consisting of other nearby cells extracellular matrix components, endogenous or exogenous soluble factors, and physical, structural, or mechanical properties of the tissues they inhabit. Can we artificially recreate tissue development such that stem cells can both self-renew and be instructed to mature properly? The main factors required to regulate the maintenance and differentiation of some types of stem cells are known. In addition, new bioengineered synthetic materials that mimic extracellular matrix components can be used as initial scaffolding for building stem cell microenvironments.

scholarly journals Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shaohua Wu ◽  
Vikas Kumar ◽  
Peng Xiao ◽  
Mitchell Kuss ◽  
Jung Yul Lim ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Cell Phenotype ◽  
Primary Concern ◽  
Ross Operation ◽  
Age Related ◽  
Cardiovascular Morbidity And Mortality ◽  
Extracellular Matrix Components ◽  
Matrix Components

AbstractHeart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.

Production of Extracellular Matrix Components in Tissue-Engineered Blood Vessels

2006 ◽  
Vol 12 (4) ◽  
pp. 831-842 ◽  
Author(s):  
Sepideh Heydarkhan-Hagvall ◽  
Maricris Esguerra ◽  
Gisela Helenius ◽  
Rigmor Söderberg ◽  
Bengt R. Johansson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Blood Vessels ◽  
Extracellular Matrix Components ◽  
Tissue Engineered Blood Vessels ◽  
Matrix Components

Guided differentiation of bone marrow stromal cells on co-cultured cartilage and bone scaffolds

Soft Matter ◽  
2015 ◽  
Vol 11 (38) ◽  
pp. 7648-7655 ◽  
Author(s):  
Paul Lee ◽  
Katelyn Tran ◽  
Gan Zhou ◽  
Asheesh Bedi ◽  
Namdev B. Shelke ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bone Scaffolds ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Osteochondral Tissue ◽  
Tissue Material

A biphasic micro and nanostructured scaffold with hydroxyapatite and extracellular matrix components was created for the regeneration of osteochondral tissue. Material cues of the biphasic scaffold supported differentiation of bone marrow stromal cells in both osteogenic and chondrogenic lineages.

scholarly journals The Role of the Extracellular Matrix Components in Cutaneous Wound Healing

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Pawel Olczyk ◽  
Łukasz Mencner ◽  
Katarzyna Komosinska-Vassev
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Growth Factors ◽  
Wound Repair ◽  
Structural Integrity ◽  
Healing Process ◽  
Cellular Functions ◽  
Extracellular Matrix Components ◽  
Essential Components ◽  
Matrix Components

Wound healing is the physiologic response to tissue trauma proceeding as a complex pathway of biochemical reactions and cellular events, secreted growth factors, and cytokines. Extracellular matrix constituents are essential components of the wound repair phenomenon. Firstly, they create a provisional matrix, providing a structural integrity of matrix during each stage of healing process. Secondly, matrix molecules regulate cellular functions, mediate the cell-cell and cell-matrix interactions, and serve as a reservoir and modulator of cytokines and growth factors’ action. Currently known mechanisms, by which extracellular matrix components modulate each stage of the process of soft tissue remodeling after injury, have been discussed.

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