Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis

The rice blast (fungal pathogen: Magnaporthe oryzae and host: Oryza sativa) is one of the most important model pathosystems for understanding plant–microbe interactions. Although both genome sequences were published as the first cases of pathogen and host, only a few in planta transcriptome data during infection are available. Due to technical difficulties, previously reported fungal transcriptome data are not highly qualified to comprehensively profile the expression of fungal genes during infection. Here, we report the high-quality transcriptomes of M. oryzae and rice during infection using a sheath infection-based RNA sequencing approach. This comprehensive expression profiling of the fungal pathogen and its host will provide a better platform for understanding the plant–microbe interactions at the genomic level and serve as a valuable resource for the research community.

Download Full-text

Systematic investigation of circular RNAs in Ascosphaera apis, a fungal pathogen of honeybee larvae

Gene ◽

10.1016/j.gene.2018.07.076 ◽

2018 ◽

Vol 678 ◽

pp. 17-22 ◽

Cited By ~ 6

Author(s):

Rui Guo ◽

Dafu Chen ◽

Huazhi Chen ◽

Zhongmin Fu ◽

Cuiling Xiong ◽

...

Keyword(s):

Fungal Pathogen ◽

Systematic Investigation ◽

Circular Rnas ◽

Ascosphaera Apis

Download Full-text

MeRIP-seq and RNA-seq data of mycelium and spore of Ascosphaera apis, a widespread bee fungal pathogen

10.1101/2020.03.16.993840 ◽

2020 ◽

Author(s):

Yu Du ◽

Haibin Jiang ◽

Zhiwei Zhu ◽

Jie Wang ◽

Huazhi Chen ◽

...

Keyword(s):

Fungal Pathogen ◽

Rna Isolation ◽

Transcript Level ◽

Current Data ◽

List Type ◽

Rna Seq ◽

Ascosphaera Apis ◽

Bee Health ◽

Intergenic Regions

ABSTRACTAscosphaera apis is widespread fungal pathogen of honeybee larvae, causing chalkbrood, a chronic disease that weakens bee health and colony productivity. In this article, mecylia and spores of A. apis were respectively purified followed by RNA isolation, cDNA library construction, MeRIP-seq and RNA-seq. A total of 62,551,172, 41,773,158, 49,535,092 and 61,569,610 raw reads were produced from Aam_IP, Aas_IP, Aam_input and Aas_input groups, respectively. After quality control, 58,484,368, 37,381,432, 44,655,434 and 58,739,742 clean reads were obtained. Furthermore, 47,706,205, 31,356,690, 35,259,810 and 44,319,061 clean reads were mapped to the reference genome of A. apis, including 39,337,036, 26,731,957, 31,987,396 and 40,017,855 unique mapped reads, and 8,369,169, 4,624,733, 3,272,414 and 4,301,206 multiple mapped reads. Among them, 96.31%, 96.51%, 96.82% and 97.11% of clean reads were mapped to exons; 2.09%, 2.31%,1.83% and 1.81% to introns; 1.60%, 1.18%, 1.35% and 1.08% to intergenic regions.Value of the dataThe data can be used to investigate the relationship between the m6A modification extent and the transcript level in the A. apis transcriptome.This dataset contributes to transcriptome-wide characterization of the m6A distributing patterns in mRNAs and non-coding RNAs in A. apis mycelium and spore.Current data benefits new functions of m6A modification in the transcripts extensively modified by m6A in A. apis mycelium and spore.Our data could be used to characterize differential patterns of the m6A methylation between mycelium and spore of A. apis.

Download Full-text

Co-incubation of dsRNA reduces proportion of viable spores of Ascosphaera apis, a honey bee fungal pathogen

Journal of Apicultural Research ◽

10.1080/00218839.2020.1754090 ◽

2020 ◽

Vol 59 (5) ◽

pp. 791-799

Author(s):

James P. Tauber ◽

Ralf Einspanier ◽

Jay D. Evans ◽

Dino P. McMahon

Keyword(s):

Honey Bee ◽

Fungal Pathogen ◽

Ascosphaera Apis

Download Full-text

Nanopore long-read transcriptome data of fungal pathogen of chalkbrood disease, Ascosphaera apis

10.1101/2020.03.12.989863 ◽

2020 ◽

Cited By ~ 5

Author(s):

Yu Du ◽

Huazhi Chen ◽

Jie Wang ◽

Zhiwei Zhu ◽

Cuiling Xiong ◽

...

Keyword(s):

Alternative Splicing ◽

Fungal Pathogen ◽

Length Distribution ◽

Full Length ◽

List Type ◽

Transcriptome Data ◽

Ascosphaera Apis ◽

Oxford Nanopore ◽

Long Read ◽

Colony Productivity

ABSTRACTAscosphaera apis is a fungal pathogen that exclusively infects honeybee larvae, leading to chalkbrood disease, which damages the number of adult honeybees and colony productivity. In this article, A. apis mecylia and spores were respectively purified followed by Oxford Nanopore sequencing via PromethION platform. In total, 6,321,704 and 6,259,727 raw reads were generated from Aam and Aas, with a length distribution among 1 kb~10 kb. The quality (Q) scores of majority of raw reads were Q9 (Aam) and Q11 (Aas). Additionally, 5,669,436 and 6,233,159 clean reads were gained, among them 79.32% and 79.62% were identified as being full-length. The lengths of redundant reads-removed full-length transcripts were among 1 kb~8 kb and 1 kb~9 kb, and most abundant length for both was 1 kb. Furthermore, the length of redundant transcripts-removed clean reads was ranged from 1 kb~7 kb, with the largest group of 1 kb. The data reported here provides a beneficial genetic resource for improving genome and transcriptome annotations of A. apis and for exploring alternative splicing and polyadenylation of A. apis mRNAs.Value of the resultCurrent dataset enables better understanding of the complexity of A. apis transcriptome.The long-read transcriptome data can be used to identify of genes and transcripts associated with A. apis infection mechanism.The accessible data provides full-length transcripts for improving gene structure and functional annotation of A. apis transcriptome.This dataset could be utilized for investigation of alternative splicing and polyadenylation of A. apis mRNAs.

Download Full-text

High mobility group (HMG-box) genes in the honeybee fungal pathogen Ascosphaera apis

Mycologia ◽

10.3852/mycologia.99.4.553 ◽

2007 ◽

Vol 99 (4) ◽

pp. 553-561 ◽

Cited By ~ 7

Author(s):

K.A. Aronstein ◽

K.D. Murray ◽

J.H. de Leon ◽

X. Qin ◽

G.M. Weinstock

Keyword(s):

Fungal Pathogen ◽

High Mobility ◽

High Mobility Group ◽

Ascosphaera Apis ◽

Hmg Box

Download Full-text

Comparative transcriptome profiling of Chinese wild grapes provides insights into powdery mildew resistance

Phytopathology ◽

10.1094/phyto-01-21-0006-r ◽

2021 ◽

Author(s):

Chen Jiao ◽

Xuepeng Sun ◽

Xiaoxiao Yan ◽

Xiaozhao Xu ◽

Qin Yan ◽

...

Keyword(s):

Fatty Acid ◽

Powdery Mildew ◽

Fungal Pathogen ◽

Fatty Acid Biosynthesis ◽

Transcriptome Profiling ◽

Wine Industry ◽

Post Inoculation ◽

Comparative Transcriptome ◽

Genes Encoding ◽

Stage Analysis

Erysiphe necator, the fungal pathogen of grape powdery mildew disease, poses a great threat to the grape fruit market and wine industry. To better understand the molecular basis of grape responses to E. necator, we performed comparative transcriptome profiling on two Chinese wild grape accessions with varying degrees of resistance to E. necator. A total of 2,856, 2,678 and 1,542 differentially expressed genes (DEGs) were identified in the susceptible Vitis pseudoreticulata ‘Hunan-1’ at 6, 24, and 96 hours post-inoculation of E. necator, respectively, while 1,921, 2,498 and 3,249 DEGs were identified in the resistant V. quinquangularis ‘Shang-24’. ‘Hunan-1’ had substantially larger fraction of down-regulated genes than ‘Shang-24’ at every infection stage. Analysis of DEGs revealed that up-regulated genes were mostly associated with defense response and disease resistance-related metabolite biosynthesis, while signaling genes were significantly suppressed in ‘Hunan-1’. Interestingly, fatty acid biosynthesis and elongation related genes were suppressed by the fungus in ‘Shang-24’ but somehow induced in ‘Hunan-1’, consistent with that E. necator is likely a fatty acid auxotroph that requires lipids from the host. Moreover, genes involved in biosynthesis and signaling of phytohormones, such as jasmonic acid and cytokinin, as well as genes encoding protein kinases and NLR proteins were differentially responded to E. necator in the two wild grapes. The variation of gene regulation associated with nutrient uptake by the fungus and with signaling transduction and pathogen recognition suggests a multi-layered regulatory network that works in concert to defend fungal pathogen infections.

Download Full-text

Water activity of the bee fungal pathogen Ascosphaera apis in relation to colony conditions

Apidologie ◽

10.1007/s13592-016-0461-7 ◽

2016 ◽

Vol 48 (2) ◽

pp. 159-167

Author(s):

Jay A. Yoder ◽

Blake W. Nelson ◽

Leighanne R. Main ◽

Andre L. Lorenz ◽

Andrew J. Jajack ◽

...

Keyword(s):

Water Activity ◽

Fungal Pathogen ◽

Ascosphaera Apis

Download Full-text

Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis

Insects ◽

10.3390/insects11070419 ◽

2020 ◽

Vol 11 (7) ◽

pp. 419

Author(s):

Zhiguo Li ◽

Mengshang Hou ◽

Yuanmei Qiu ◽

Bian Zhao ◽

Hongyi Nie ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Honey Bee ◽

Fungal Pathogen ◽

Disease Diagnosis ◽

Enzyme Linked Immunosorbent Assay ◽

Antioxidant Enzyme Activities ◽

Ascosphaera Apis ◽

Specific Activities ◽

Honey Bee Larvae

The fungus Ascosphaera apis, an obligate fungal pathogen of honey bee brood, causes chalkbrood disease in honey bee larvae worldwide. Biological characteristics of the fungal pathogen and the molecular interactions between A. apis and honey bees have been studied extensively. However, little is known about the effects of A. apis infection on antioxidant enzyme activities and metabolic profiles of the gut of honey bee larvae. In this study, sandwich enzyme-linked immunosorbent assay and LC-MS based untargeted metabolomic analysis were employed to determine the changes in the specific activities of antioxidant enzymes and the metabolomic profiles in gut tissues of A. apis-infected larvae (105 A. apis spores per larva) and controls. Results showed that specific activities of superoxide dismutase, catalase and glutathione S-transferase were significantly higher in the guts of the control larvae than in the guts of the A. apis-infected larvae. The metabolomic data revealed that levels of 28 and 52 metabolites were significantly higher and lower, respectively, in the guts of A. apis-infected larvae than in the guts of control larvae. The 5-oxo-ETE level in the infected larvae was two times higher than that in the control larvae. Elevated 5-oxo-ETE levels may act as a potential metabolic biomarker for chalkbrood disease diagnosis, suggesting that A. apis infection induced obvious oxidative stress in the honey bee larvae. The levels of metabolites such as taurine, docosahexaenoic acid, and L-carnitine involved in combating oxidative stress were significantly decreased in the gut of A. apis-infected larvae. Overall, our results suggest that A. apis infection may compromise the ability of infected larvae to cope with oxidative stress, providing new insight into changing patterns of physiological responses to A. apis infection in honey bee larvae by concurrent use of conventional biochemical assays and untargeted metabolomics.

Download Full-text