scholarly journals The silk of gorse spider mite Tetranychus lintearius represents a novel natural source of nanoparticles and biomaterials

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Antonio Abel Lozano-Pérez ◽  
Ana Pagán ◽  
Vladimir Zhurov ◽  
Stephen D. Hudson ◽  
Jeffrey L. Hutter ◽  
...  
Keyword(s):  
Spider Mite ◽  
Biomedical Application ◽  
Attenuated Total Reflectance ◽  
Cell Cytoplasm ◽  
Total Reflectance ◽  
Silk Production ◽  
Young’S Moduli ◽  
Mammalian Cell Growth ◽  
Silkworm Silk

Abstract Spider mites constitute an assemblage of well-known pests in agriculture, but are less known for their ability to spin silk of nanoscale diameters and high Young’s moduli. Here, we characterize silk of the gorse spider mite Tetranychus lintearius, which produces copious amounts of silk with nano-dimensions. We determined biophysical characteristics of the silk fibres and manufactured nanoparticles and biofilm derived from native silk. We determined silk structure using attenuated total reflectance Fourier transform infrared spectroscopy, and characterized silk nanoparticles using field emission scanning electron microscopy. Comparative studies using T. lintearius and silkworm silk nanoparticles and biofilm demonstrated that spider mite silk supports mammalian cell growth in vitro and that fluorescently labelled nanoparticles can enter cell cytoplasm. The potential for cytocompatibility demonstrated by this study, together with the prospect of recombinant silk production, opens a new avenue for biomedical application of this little-known silk.

In Vitro Study on Bleached Enamel

2007 ◽  
Vol 361-363 ◽  
pp. 833-836
Author(s):  
Xiao Jie Wang ◽  
Boriana Mihailova ◽  
Arndt Klocke ◽  
Stefanie Heidrich ◽  
Ulrich Bismayer
Keyword(s):  
Citric Acid ◽  
Aqueous Solutions ◽  
Electron Probe ◽  
In Vitro Study ◽  
Superficial Layer ◽  
Commercial Product ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Surface Crystal

The surface crystal chemistry of enamel treated with peroxide-based (the commercial product Opalescence Xtra Boost as well as aqueous solutions of H2O2) and citric-acid-containing bleaching agents (the commercial product Rapid White as well as aqueous solutions of C6H8O7) were studied by Attenuated Total Reflectance Infrared Spectroscopy and electron-probe microanalysis. The results show that the treatment with H2O2-based reagents leads to no side effects on the enamel superficial layer, whereas C6H8O7-based agents deteriorate the enamel mineral component. Hence, bleaching with citric-acid-containing products cannot be recommended.

scholarly journals Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Sung Eun Kim ◽  
Young-Pil Yun ◽  
Deok-Won Lee ◽  
Eun Young Kang ◽  
Won Jae Jeong ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Biphasic Calcium Phosphate ◽  
Bone Substitutes ◽  
Calcium Deposition ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
X Ray ◽  
Stimulation Of

Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). Anin vitrorelease study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days.In vitroresults revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

Attenuated total reflectance/Fourier transform infrared (ATR/FTIR) mapping coupled with principal component analysis for the study of in vitro degradation of porous polylactide/hydroxyapatite composite material

2014 ◽  
Vol 6 (15) ◽  
pp. 5590-5595 ◽  
Author(s):  
Nan Jing ◽  
Xiaoting Jiang ◽  
Qian Wang ◽  
Yongjiao Tang ◽  
Pudun Zhang
Keyword(s):  
Principal Component Analysis ◽  
Composite Material ◽  
Fourier Transform ◽  
Principal Component ◽  
Component Analysis ◽  
Attenuated Total Reflectance ◽  
In Vitro Degradation ◽  
Total Reflectance ◽  
Hydroxyapatite Composite

We proposes coupling ATR/FTIR mapping with principal component analysis for the biomimetic degradation of poly(l-lactide)/hydroxyapatite composite material.

The Utilization of Ftir (Fourier Transform Infra-Red) Method Combined with Chemometrics For Authentication of Indonesian Coffee Powder

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Firmansyah A. ◽  
Winingsih W. ◽  
Soebara Y S
Keyword(s):  
Fourier Transform ◽  
Natural Product ◽  
High Performance ◽  
Principal Component ◽  
Raw Material ◽  
Attenuated Total Reflectance ◽  
Coffee Bean ◽  
Product Analysis ◽  
Total Reflectance ◽  
And Cluster Analysis

Analysis of natural product remain challenging issues for analytical chemist, since natural products are complicated system of mixture. The most popular methods of choice used for quality control of raw material and finished product are high performance liquid chromatography (HPLC), gas chromatography (GC) and mass spectrometry (MS). The utilization of FTIR-ATR (Fourier Transform Infrared-Attenuated Total Reflectance) method in natural product analysis is still limited. This study attempts to expand the use of FTIR spectroscopy in authenticating Indonesian coffee powder.The coffee samples studied were taken from nine regions in Indonesia, namely Aceh Gayo, Flores, Kintamani, Mandheling, Papua, Sidikalang, Toraja, Kerinci and Lampung.The samples in the form of coffee bean from various regions were powdered . The next step conducted was to determine the spectrum using the FTIR-ATR (Attenuated Total Reflectance) using ZnSe crystal of 8000 resolution. Spectrum samples, then, were analyzed using chemometrics. The utilized chemometric model was the principal component analysis (PCA) and cluster analysis (CA). Based on the chemometric analysis, there are similarities between Aceh Gayo coffee with Toraja coffee, Mandailing coffee, Kintamani coffee and Flores coffee. Sidikalang coffee has a similarity to Flores coffee; Papua coffee has a similarity to Sidikalang coffee; Lampung coffee has a similarity to Sidikalang coffee, while Kerinci coffee has a similarity to Papua coffee.

Quality evaluation of tugaksheeree samples by ATR-FTIR spectroscopy using multicomponent analysis

2020 ◽  
Vol 10 ◽  
Author(s):  
Nikunj D. Patel ◽  
Niranjan S. Kanaki
Keyword(s):  
Principal Component Analysis ◽  
Cluster Analysis ◽  
Quality Evaluation ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Attenuated Total Reflectance ◽  
Chemometric Methods ◽  
Total Reflectance ◽  
Fingerprint Region ◽  
Ftir Technique

Background: Numerous Ayurvedic formulations contains tugaksheeree as key ingredient. Tugaksheereeis the starch gained from the rhizomes of two plants, Curcuma angustifoliaRoxb. (Zingiberaceae) and Marantaarundinacea (MA) Linn. (Marantaceae). Objective: The primary concerns in quality assessment of Tugaksheeree occur due to adulteration or substitution. Method: In current study, Fourier transform infrared (FTIR) technique with attenuated total reflectance (ATR) facility was used to evaluate tugaksheeree samples. Total 10 different samples were studied and transmittance mode was kept to record the spectra devoid of pellets of KBR. Further treatment was given with multi component tools by considering fingerprint region of the spectra. Multivariate analysis was performed by various chemometric methods. Result: Multi component methods like Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA)were used to discriminate the tugaksheeree samples using Minitab software. Conclusion: This method can be used as a tool to differentiate samples of tugaksheeree from its adulterants and substitutes.

Negatively Charged Ions to Probe Self-Assembled Monolayers Reorganization Driven by Interchain Interactions

2021 ◽  
Author(s):  
Angelo Tricase ◽  
Angela Stefanachi ◽  
Rosaria Anna Anna Picca ◽  
Eleonora Macchia ◽  
Alessandro Favia ◽  
...  
Keyword(s):  
Grazing Angle ◽  
Self Assembled Monolayers ◽  
Attenuated Total Reflectance ◽  
Interchain Interaction ◽  
Total Reflectance ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Interchain Interactions ◽  
Ir Study ◽  
Charged Ions

A combined cyclic voltammetry (CV) and grazing angle – attenuated total reflectance (GA-ATR) IR study on the interchain interaction driven reorganization of self-assembled monolayers (SAMs) in an electric field, is...

Detection of bloodstains using attenuated total reflectance-Fourier transform infrared spectroscopy supported with PCA and PCA–LDA

2021 ◽  
pp. 002580242110109
Author(s):  
Sweety Sharma ◽  
Rito Chophi ◽  
Jaskirandeep Kaur Jossan ◽  
Rajinder Singh
Keyword(s):  
Fourier Transform ◽  
Ftir Spectroscopy ◽  
Body Fluid ◽  
Due Process ◽  
Dna Analysis ◽  
Fourier Transform Infrared ◽  
Body Fluids ◽  
Attenuated Total Reflectance ◽  
Total Reflectance ◽  
Non Destructive

The most important task in a criminal investigation is to detect and identify the recovered biological stains beyond reasonable scientific doubt and preserve the sample for further DNA analysis. In the light of this fact, many presumptive and confirmatory tests are routinely employed in the forensic laboratories to determine the type of body fluid. However, the currently used techniques are specific to one type of body fluid and hence it cannot be utilized to differentiate multiple body fluids. Moreover, these tests consume the samples in due process, and thus it becomes a great limitation especially considering the fact that samples are recovered in minute quantity in forensic cases. Therefore, such limitations necessitate the use of non-destructive techniques that can be applied simultaneously to all types of bodily fluids and allow sample preservation for further analysis. In the current work, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy has been used to circumvent the aforementioned limitations. The important factors which could influence the detection of blood such as the effect of substrates, washing/chemical treatment, ageing, and dilution limits on the analysis of blood have been analysed. In addition, blood discrimination from non-blood substance (biological and non-biological in nature) has also been studied. Chemometric technique that is PCA–LDA has been used to discriminate blood from other body fluids and it resulted in 100% accurate classification. Furthermore, blood and non-blood substances including fake blood have also been classified into separate clusters with a 100% accuracy, sensitivity, and specificity. All-inclusive, this preliminary study substantiates the potential application of ATR-FTIR spectroscopy for the non-destructive identification of blood traces in simulated forensic casework conditions with 0% rate of false classification.

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