scholarly journals Piglets can secrete acidic mammalian chitinase from the pre weaning stage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kiyonori Kawasaki ◽  
Tomomi Osafune ◽  
Saya Tamehira ◽  
Kiminobu Yano
Keyword(s):  
Gene Expression ◽  
Growth Stages ◽  
Pork Production ◽  
Stomach Tissue ◽  
Degrading Enzymes ◽  
Its Gene ◽  
Mrna And Protein Expression ◽  
Acidic Mammalian Chitinase ◽  
Weaning Piglets ◽  
Fattening Pigs

AbstractFishmeal substitutes (such as insect-based feeds) in pig diets can promote sustainable pork production. Insect powders contain chitin, a nitrogen-containing indigestible material, and pigs must have the capacity to secrete chitin-degrading enzymes to benefit from these diets. The chitin-degrading enzyme (acidic mammalian chitinase; AMCase) and its gene expression have been detected in the stomach tissue of approximately 6-month-old fattening pigs; however, it remains unclear from which stage chitin-degrading enzymes are secreted. In the present study, the stomach tissue of piglets was collected from the suckling stage (14 d old) to 56 d to evaluate chitin-degrading enzymes and associated gene expression. AMCase mRNA and protein expression was detected in the stomach tissue of all piglets from days 14 to 56. AMCase secretion might increase with the increase in stomach tissue weight as piglets grow. Insect powders can therefore be used in the diets of pre-weaning piglets. The gastric AMCase level was approximately 30% that of fattening pigs. The appropriate inclusion of insect meals in the diets of pigs at different growth stages still needs to be determined.

scholarly journals GhKWL1 Upregulates GhERF105 but Its Function Is Impaired by Binding with VdISC1, a Pathogenic Effector of Verticillium dahliae

2021 ◽  
Vol 22 (14) ◽  
pp. 7328
Author(s):  
Yang Chen ◽  
Mi Zhang ◽  
Lei Wang ◽  
Xiaohan Yu ◽  
Xianbi Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Plants ◽  
Gossypium Hirsutum ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Defense Response ◽  
Upland Cotton ◽  
Effector Protein ◽  
Positive Regulator ◽  
Its Gene

Verticillium wilt, caused by Verticillium dahliae, is a devastating disease for many important crops, including cotton. Kiwellins (KWLs), a group of cysteine-rich proteins synthesized in many plants, have been shown to be involved in response to various phytopathogens. To evaluate genes for their function in resistance to Verticillium wilt, we investigated KWL homologs in cotton. Thirty-five KWL genes (GhKWLs) were identified from the genome of upland cotton (Gossypium hirsutum). Among them, GhKWL1 was shown to be localized in nucleus and cytosol, and its gene expression is induced by the infection of V. dahliae. We revealed that GhKWL1 was a positive regulator of GhERF105. Silencing of GhKWL1 resulted in a decrease, whereas overexpression led to an increase in resistance of transgenic plants to Verticillium wilt. Interestingly, through binding to GhKWL1, the pathogenic effector protein VdISC1 produced by V. dahliae could impair the defense response mediated by GhKWL1. Therefore, our study suggests there is a GhKWL1-mediated defense response in cotton, which can be hijacked by V. dahliae through the interaction of VdISC1 with GhKWL1.

scholarly journals Transcriptome Analysis of Differentially Expressed mRNA Related to Pigeon Muscle Development

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2311
Author(s):  
Hao Ding ◽  
Yueyue Lin ◽  
Tao Zhang ◽  
Lan Chen ◽  
Genxi Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Differentially Expressed Genes ◽  
Growth And Development ◽  
Muscle Development ◽  
Expression Patterns ◽  
Differentially Expressed ◽  
Pathway Enrichment Analysis ◽  
Growth Stages ◽  
Gene Expression And Regulation

The mechanisms behind the gene expression and regulation that modulate the development and growth of pigeon skeletal muscle remain largely unknown. In this study, we performed gene expression analysis on skeletal muscle samples at different developmental and growth stages using RNA sequencing (RNA−Seq). The differentially expressed genes (DEGs) were identified using edgeR software. Weighted gene co−expression network analysis (WGCNA) was used to identify the gene modules related to the growth and development of pigeon skeletal muscle based on DEGs. A total of 11,311 DEGs were identified. WGCNA aggregated 11,311 DEGs into 12 modules. Black and brown modules were significantly correlated with the 1st and 10th day of skeletal muscle growth, while turquoise and cyan modules were significantly correlated with the 8th and 13th days of skeletal muscle embryonic development. Four mRNA−mRNA regulatory networks corresponding to the four significant modules were constructed and visualised using Cytoscape software. Twenty candidate mRNAs were identified based on their connectivity degrees in the networks, including Abca8b, TCONS−00004461, VWF, OGDH, TGIF1, DKK3, Gfpt1 and RFC5, etc. A KEGG pathway enrichment analysis showed that many pathways were related to the growth and development of pigeon skeletal muscle, including PI3K/AKT/mTOR, AMPK, FAK, and thyroid hormone pathways. Five differentially expressed genes (LAST2, MYPN, DKK3, B4GALT6 and OGDH) in the network were selected, and their expression patterns were quantified by qRT−PCR. The results were consistent with our sequencing results. These findings could enhance our understanding of the gene expression and regulation in the development and growth of pigeon muscle.

Fat feeding impairs glycogen synthase activity in mice without effects on its gene expression

Metabolism ◽  
2003 ◽  
Vol 52 (5) ◽  
pp. 535-539 ◽  
Author(s):  
Xudong Huang ◽  
Mona Hansson ◽  
Esa Laurila ◽  
Bo Ahrén ◽  
Leif Groop
Keyword(s):  
Gene Expression ◽  
Glycogen Synthase ◽  
Its Gene ◽  
Fat Feeding ◽  
Glycogen Synthase Activity

scholarly journals Dexamethasone administration attenuates the inhibitory effect of lipopolysaccharide on IGF-I and IGF-binding protein-3 in adult rats

2005 ◽  
Vol 185 (3) ◽  
pp. 467-476 ◽  
Author(s):  
Teresa Priego ◽  
Miriam Granado ◽  
Ana Isabel Martín ◽  
Asunción López-Calderón ◽  
María Angeles Villanúa
Keyword(s):  
Gene Expression ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Serum Concentrations ◽  
Gh Receptor ◽  
Its Gene ◽  
Igf I ◽  
Igf Binding ◽  
Igf Binding Protein ◽  
Igfbp 3

The aim of this study was to investigate whether glucocorticoid administration had a beneficial effect on serum concentrations of insulin-like growth factor I (IGF-I) and on IGF-binding protein 3 (IGFBP-3) in rats injected with lipopolysaccharide (LPS). Adult male rats were injected with LPS or saline and pretreated with dexamethasone or saline. Dexamethasone administration decreased growth hormone (GH) receptor and IGF-I mRNA levels in the liver of control rats. LPS decreased GH receptor and IGF-I gene expression in the liver of saline-treated rats but not in the liver of dexamethasone-pretreated rats. In the kidney, GH receptor mRNA levels were not modified by dexamethasone or LPS treatment. However, LPS decreased renal IGF-I gene expression and dexamethasone pretreatment prevented this decrease. Serum concentrations of IGF-I were decreased by LPS, and dexamethasone pretreatment attenuated this effect. The gene expression of IGFBP-3 in the liver and kidney and its circulating levels were decreased by LPS. In control rats dexamethasone increased circulating IGFBP-3 and its gene expression in the liver, and decreased the proteolysis of this protein. Dexamethasone pretreatment attenuated the LPS-induced decrease in IGFBP-3 gene expression in the liver and prevented the LPS-induced decrease in IGFBP-3 gene expression in the kidney. Moreover, dexamethasone pretreatment attenuated the LPS-induced decrease in serum concentrations of IGFBP-3 and decreased the LPS-induced IGFBP-3 proteolysis in serum. In conclusion, dexamethasone pretreatment partially attenuates the inhibitory effect of LPS on serum IGF-I by blocking the decrease of its gene expression in the kidney as well as by attenuating the decrease in serum concentrations of IGFBP-3.

GENE EXPRESSION OF MATRIX-DEGRADING ENZYMES IN PATELLAR TENDINOPATHY

2013 ◽  
Vol 47 (9) ◽  
pp. e2.3-e2
Author(s):  
T Samiric ◽  
C J Handley ◽  
J Cook ◽  
J Parkinson
Keyword(s):  
Gene Expression ◽  
Patellar Tendinopathy ◽  
Degrading Enzymes ◽  
Matrix Degrading Enzymes

Differential effects of early-life and post-weaning galactooligosaccharides intervention on colonic bacterial composition and function in weaning piglets

2021 ◽  
Author(s):  
Shiyi Tian ◽  
Jue Wang ◽  
Jing Wang ◽  
Weiyun Zhu
Keyword(s):  
Gene Expression ◽  
Early Life ◽  
Short Chain Fatty Acids ◽  
Combined Effect ◽  
Economic Losses ◽  
Swine Industry ◽  
Bacterial Composition ◽  
Colonic Microbiota ◽  
Α Diversity ◽  
Weaning Piglets

Recently, we have proved that the early-life galactooligosaccharides (GOS) intervention could improve the colonic function by altering the bacterial composition in the suckling piglets. However, whether the early-life GOS (ELG) intervention could have a long influence of the colonic microbiota, and the ELG and post-weaning GOS (PWG) combined intervention would have an interaction effect on maintaining colonic health in weaning piglets remain to be explored. Thus in this study, we illustrated the differential effect of ELG and PWG intervention on colonic microbiota and colonic function of weaning piglets. Our results showed that both the ELG and PWG intervention decreased the diarrhea frequency of weaning piglets, while the PWG intervention increased colonic indexes. After 16S rRNA MiSeq sequencing of gut bacteria belonged to colonic niches (mucosa and digesta), the PWG increased the α-diversity of colonic mucosal bacteria was revealed. In addition, we found both the ELG and PWG intervention enriched the abundance of short chain fatty acids (SCFAs) producer in different colonic niches and increased total SCFAs concentrations in colonic digesta. These changes selectively modulated the mRNA expression of pattern recognition receptors and barrier proteins in the colonic mucosa. Of note, the combined effect of ELG and PWG effectively enhanced colonic SCFAs producer enrichment and up-regulated the butyrate concentration. Meanwhile, the gene expression of MyD88-NFκB signaling and the pro-inflammatory cytokines contents were markedly reduced under the combined effect of ELG and PWG. Importance Reducing the disorders of gut ecosystem is an effective way to relieve weaning stresses of piglets and save economic losses in the modern swine industry. To this end, prebiotics were often added in diet during the weaning transition. In present study, we demonstrated that the ELG and PWG intervention had shown different effects on the bacterial composition of different colonic niches and colonic function in the weaning piglets. Especially under the combined effect of ELG and PWG intervention, the gene expression of MyD88-NFκB signaling and the contents of pro-inflammation cytokines decreased with the increasing concentration of butyrate, which is one of the important microbial metabolites in the colon of weaning piglets. These findings further provided new insights into nutritional interventions to alleviate intestinal ecosystem dysbiosis and gut dysfunction in the piglets during the weaning transition.

scholarly journals First draft genome sequence of a strain belonging to the Zoogloea genus and its gene expression in situ

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Emilie E. L. Muller ◽  
Shaman Narayanasamy ◽  
Myriam Zeimes ◽  
Cédric C. Laczny ◽  
Laura A. Lebrun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Its Gene

scholarly journals Glucose Signaling Pathway and Growth Conditions Regulate Gene Expression in Retrotransposon Ty2

2009 ◽  
Vol 64 (7-8) ◽  
pp. 526-532 ◽  
Author(s):  
Sezai Türkel ◽  
Özgür Bayram ◽  
Elif Arık
Keyword(s):  
Gene Expression ◽  
Growth Conditions ◽  
Glucose Sensors ◽  
Yeast Cells ◽  
Growth Stages ◽  
Regulate Gene Expression ◽  
Glucose Signaling ◽  
Yeast Cultures ◽  
Membrane Bound ◽  
High Level

Gene expression in the yeast retrotransposon Ty2 is regulated at transcriptional and translational levels. In this study, we have shown that the transcription of Ty2 is partially dependent on the membrane-bound glucose sensors Gpr1p and Mth1p in Saccharomyces cerevisiae. Transcription of Ty2 decreased approx. 3-fold in the gpr1, mth1 yeast mutant. Moreover, our results revealed that the transcription of Ty2 fluctuates during the growth stages of S. cerevisae. Both transcription and the frameshift rate of Ty2 rapidly dropped when the stationary stage yeast cells were inoculated into fresh medium. There was an instant activation of Ty2 transcription and a high level expression during the entire logarithmic stage of yeast growth. However, the transcription of Ty2 decreased 2-fold when the yeast cultures entered the stationary stage. The frameshift rate in Ty2 also varied depending on the growth conditions. The highest frameshift level was observed during the mid-logarithmic stage. It decreased up to 2-fold during the stationary stage. Furthermore, we have found that the frameshift rate of Ty2 diminished at least 5-fold in slowly growing yeasts. These results indicate that the transcription and the frameshift efficiency are coordinately regulated in the retrotransposon Ty2 depending on the growth conditions of S. cerevisiae.

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