scholarly journals Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Myoung Hwa Kim ◽  
Jeong-Rim Lee ◽  
Ki-Joon Kim ◽  
Ji Hae Jun ◽  
Hye Jeong Hwang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nk Cell ◽  
Cell Activity ◽  
Treated Group ◽  
Control Group ◽  
Nk Cell Activity ◽  
Clinical Dose ◽  
Group 2 ◽  
Mcf 7 ◽  
Group 1

AbstractIn our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the anti-tumor effect of tramadol in vivo. Female nude mice orthotopically inoculated with luciferase-expressing MCF-7 cells, were randomly divided into the control (saline), tramadol group 1 (1.5 mg kg−1 day−1), tramadol group 2 (3 mg kg−1 day−1), and morphine (0.5 mg kg−1 day−1) (n = 5/group). Bioluminescence signals after D-luciferin injection, tumor size, and tumor weight were compared among groups after 4 weeks. Estrogen receptor (ER), progesterone receptor (PR), and transient receptor potential vanilloid (TRPV)-1 expression, natural killer (NK) cell activity, and serum interleukin (IL)-1β, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-6 were then examined. Tumour growth was attenuated in tramadol-treated groups (P < 0.05). NK cell activity was significantly decreased only in the morphine treated group not in sham, control, and tramadol groups. The expression levels of ERα, PRα and β, and TRPV1 were decreased in tramadol group 2 compared with those in the morphine group, but not compared to the control group. Serum levels of IL-6 and TNFα were reduced in both tramadol-treated group 1 and 2 compared to the control group. Overall, clinical dose of tramadol has anti-tumour effects on MCF-7 cell-derived breast cancer in a xenograft mouse model.

Analysis of the Effect of Psychological Nursing Intervention on Perioperative Mood and NK Cell Activity in Breast Cancer Patients

2021 ◽  
Vol 7 (5) ◽  
pp. 3301-3314
Author(s):  
Jihong Yuan ◽  
Linghong Yuan ◽  
Yi Hu ◽  
Jingjing Gong ◽  
Yang Tong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Nk Cell ◽  
Psychological Intervention ◽  
Cell Activity ◽  
Nursing Intervention ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Nk Cell Activity ◽  
Intervention Measures

Breast cancer (Breast Cancer, BC) is one of the most common malignant tumors in women in the world. It is the first malignant tumor that causes the death of women in developing countries. It seriously threatens the lives and health of women and causes damage to the family, economy and society. Through psychological nursing intervention, it has a positive effect on the perioperative mood and NK cell activity of breast cancer patients. The purpose of this article is to explore the effects of psychological intervention on the perioperative mood and NK cell activity of breast cancer patients. This article is based on the concept and theory of psychological nursing intervention for female breast cancer patients, and establishes personalized intervention measures in conjunction with clinical practice to intervene the emotions of breast cancer patients during the perioperative period. This article analyzes the nature, intensity, and causes of negative psychology of the subject through in-depth understanding of the subject’s psychological emotions, coping ability, personality characteristics, past emotional experience, and social support, and discovers the different emotional characteristics of the patient, and formulates the personality psychological intervention measures to stimulate positive and optimistic attitudes and ease the degree of negative psychology. This article analyzes the psychological characteristics of several breast cancer patients. Take corresponding psychological intervention measures. Cope with a series of bad psychology caused by image damage caused by total mastectomy. The results and data in this article show that the incidence of anxiety and depression in the control group is higher than that of the psychological intervention group at 7 days after the operation, with P values of 0.0059 and 0. 0215.Psychological intervention reduces the incidence of negative emotions and reduces the negativeness of patients. The intensity of emotion has played a good clinical effect.

Analysis of the Effect of Psychological Nursing Intervention on Perioperative Mood and NK Cell Activity in Breast Cancer Patients

2021 ◽  
Vol 7 (4) ◽  
pp. 443-456
Author(s):  
Jihong Yuan ◽  
Linghong Yuan ◽  
Yi Hu ◽  
Jingjing Gong ◽  
Yang Tong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Nk Cell ◽  
Psychological Intervention ◽  
Cell Activity ◽  
Nursing Intervention ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Nk Cell Activity ◽  
Intervention Measures

Breast cancer (Breast Cancer, BC) is one of the most common malignant tumors in women in the world. It is the first malignant tumor that causes the death of women in developing countries. It seriously threatens the lives and health of women and causes damage to the family, economy and society. Through psychological nursing intervention, it has a positive effect on the perioperative mood and NK cell activity of breast cancer patients. The purpose of this article is to explore the effects of psychological intervention on the perioperative mood and NK cell activity of breast cancer patients. This article is based on the concept and theory of psychological nursing intervention for female breast cancer patients, and establishes personalized intervention measures in conjunction with clinical practice to intervene the emotions of breast cancer patients during the perioperative period. This article analyzes the nature, intensity, and causes of negative psychology of the subject through in - depth understanding of the subject's psychological emotions, coping ability, personality characteristics, past emotional experience, and social support, and discovers the different emotional characteristics of the patient, and formulates the personality psychological intervention measures to stimulate positive and optimistic attitudes and ease the degree of negative psychology. This article analyzes the psychological characteristics of several breast cancer patients. Take corresponding psychological intervention measures. Cope with a series of bad psychology caused by image damage caused by total mastectomy. The results and data in this article show that the incidence of anxiety and depression in the control group is higher than that of the psychological intervention group at 7 days after the operation, with P values of 0.0059 and 0. 0215. Psychological intervention reduces the incidence of negative emotions and reduces the negativeness of patients. The intensity of emotion has played a good clinical effect.

Immune Responses to Guided Imagery During Breast Cancer Treatment

2008 ◽  
Vol 9 (3) ◽  
pp. 205-214 ◽  
Author(s):  
Cecile A. Lengacher ◽  
Mary P. Bennett ◽  
Lois Gonzalez ◽  
Danielle Gilvary ◽  
Charles E. Cox ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nk Cells ◽  
Guided Imagery ◽  
Nk Cell ◽  
Intervention Group ◽  
Cell Activity ◽  
Control Group ◽  
Cell Cytotoxicity ◽  
Nk Cell Activity ◽  
Nk Cell Cytotoxicity

Background: The use of relaxation and guided imagery to reduce stress and improve immune function has great potential benefits for patients with breast cancer. Methods: This pilot study used a pretest—posttest experimental design with 28 breast cancer patients, aged 25 to 75 years, with the diagnosis of stage 0, 1, or 2 breast cancer. The experimental group received a relaxation and guided imagery intervention and the control group received standard care. The effects of the intervention on immune function were measured by natural killer (NK) cell cytotoxicity and IL-2—activated NK cell activity prior to surgery and 4 weeks postsurgery. NK cell activity was measured using a 15-hr incubation chromium release assay. Cytotoxicity of NK cells was measured against chromium-labeled K-562 target cells. IL-2 was used to enhance reactivity of NK cells against tumor cells. After incubation for 15 hr, cytotoxicity was measured through the release of radioactive chromium. Results: Significant differences between groups were found at 4 weeks postsurgery. T-tests showed increased NK cell cytotoxicity for the intervention group at 100:1, 50:1, and 25:1 effector cell: target cell ratios (E:T) ( p < .01 to p < .05) and increased activation for IL-2 at 100:1, 50:1, 25:1, and 12.5:1 (E:T) ( p < .01 to p < .05) for the intervention group as compared to the control group. Discussion: These findings suggest that a relaxation intervention such as guided imagery could have an effect on NK cell cytotoxicity and NK cell cytotoxicity after activation with IL-2 in patients undergoing surgery for breast cancer.

scholarly journals Immunomodulatory Effects of Perioperative Dexmedetomidine in Ovarian Cancer: An In Vitro and Xenograft Mouse Model Study

2021 ◽  
Vol 11 ◽  
Author(s):  
Seokyung Shin ◽  
Ki Jun Kim ◽  
Hye Jeong Hwang ◽  
Sewon Noh ◽  
Ju Eun Oh ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Ovarian Cancer ◽  
Mouse Model ◽  
Cell Viability ◽  
Nk Cell ◽  
Cell Activity ◽  
Tumor Burden ◽  
Control Group ◽  
Nk Cell Activity ◽  
Dexmedetomidine Infusion

BackgroundThe surgical stress response (SSR) causes immunosuppression which may cause residual tumor growth and micrometastasis after cancer surgery. We investigated whether dexmedetomidine affects cancer cell behavior and immune function in an ovarian cancer xenograft mouse model.MethodsThe effect of dexmedetomidine on cell viability and cell cycle was assessed using SK-OV-3 cells at drug concentrations of 0.5, 0.1, 5, and 10 µg mL-1. BALB/c nude mice were used for the ovarian cancer model with the Dexmedetomidine group (n=6) undergoing surgery with dexmedetomidine infusion and the Control group (n=6) with saline infusion for 4 weeks. Natural killer (NK) cell activity, serum proinflammatory cytokines, and cortisol were measured at predetermined time points and tumor burden was assessed 4 weeks after surgery.ResultsDexmedetomidine had no effect on cell viability or cell cycle. Following a sharp decrease on postoperative day (POD) 1, NK cell activity recovered faster in the Dexmedetomidine group with significant difference vs. the Control group on POD 3 (P=0.028). In the Dexmedetomidine group, cortisol levels were lower on POD 3 (P=0.004) and TNF-α levels were lower at 4 weeks after surgery (P&lt;0.001) compared to the Control group. The Dexmedetomidine group showed lower tumor burden at 4 weeks vs. the Control group as observed by both tumor weight (P&lt;0.001) and the in vivo imaging system (P=0.03).ConclusionsDexmedetomidine infusion may improve ovarian cancer surgery outcome by suppressing the SSR and stress mediator release. Further studies are needed to elucidate the mechanisms by which dexmedetomidine acts on cancer and immune cells.

Lymphocyte Subpopulations in Patients with Advanced Breast Cancer Submitted to Neoadjuvant Chemotherapy

2000 ◽  
Vol 86 (5) ◽  
pp. 403-407 ◽  
Author(s):  
Eddie Fernando Candido Murta ◽  
Jurandyr Moreira de Andrade ◽  
Roberto Passeto Falcio ◽  
Sérgio Bighetti
Keyword(s):  
Breast Cancer ◽  
Neoadjuvant Chemotherapy ◽  
Advanced Breast Cancer ◽  
B Lymphocytes ◽  
Interleukin 2 ◽  
Advanced Breast ◽  
Control Group ◽  
Percentage Increase ◽  
Group 2 ◽  
Group 1

Aims and background There is an enhanced immune response in patients with breast cancer after the use of chemotherapy. The objective of this study was therefore to investigate alterations in the number of peripheral lymphocytes in patients with breast cancer after neoadjuvant chemotherapy (NC) and the relationship with prognosis. Methods Thirty women were analyzed. Their UICC staging was IIb (only T3N0 included) and III (N3 not included). Sample analysis was performed using flow cytometry before the first cycle and 18 to 21 days after the last cycle of NC. The lymphocyte subsets studied were: T (CD3, CD4, CD8), B (CD19, CD23), natural killer (NK) (CD56, CD16), and interleukin-2 (CD25). CD3, CD56, CD8, and CD16 lymphocytes were analyzed with double marking. After x = 3.8 ± 1.3 cycles of 5-fluorouracil, epirubicin and cyclophosphamide (FEC), 16 patients showed a complete or partial response (group 1). After three cycles 14 showed no response or tumor progression (group 2). A control group of healthy women was used for pretreatment analysis. Results Before NC there was a significant increase in B lymphocytes and NK cells in comparison to the control group. After NC there was a significant percentage increase in CD3, CD4, CD8, CD25 and CD3+CD56+ cells and a decrease in CD19, CD23, CD56, CD16 and CD16+CD8+ cells. There was a significant fall in the absolute number of CD4, CD19, CD23, CD56, CD16 and CD16+CD8+ lymphocytes and an increase in GD3+CD56+ lymphocytes. Before NC the ratio CD4/CD8 in group 1 was 2.25 ± 0.5 and in group 2 it was 1.79 ± 0.5 (P <0.05). Conclusions Patients with advanced breast cancer showed increases in B and NK lymphocytes. Neoadjuvant chemotherapy (FEC) caused an increase in CD3+CD56+ and a decrease in B lymphocytes. Patients with an increased CD4/CD8 ratio have a better chance of responding to neoadjuvant chemotherapy.

5108 POSTER Association of Decreased NK Cell Activity and IFNgamma Expression With pSTAT Dysregulation in Breast Cancer Patients

2011 ◽  
Vol 47 ◽  
pp. S362 ◽  
Author(s):  
S. Radenkovic ◽  
G. Konjevic ◽  
T. Srdic Rajic ◽  
L. Stamatovic ◽  
M. Milovic ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Nk Cell ◽  
Cell Activity ◽  
Breast Cancer Patients ◽  
Nk Cell Activity

Effect of eccentric exercise on natural killer cell activity

1995 ◽  
Vol 78 (4) ◽  
pp. 1442-1446 ◽  
Author(s):  
J. Palmo ◽  
S. Asp ◽  
J. R. Daugaard ◽  
E. A. Richter ◽  
M. Klokker ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Eccentric Exercise ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Activity ◽  
Control Group ◽  
Systemic Effects ◽  
Nk Cell Activity ◽  
Blood Samples

The effect of eccentric one-legged exercise on natural killer (NK) cell activity was studied in eight healthy males. To distinguish between local and systemic effects, blood samples were collected from veins in the exercising leg and resting arm. However, the results did not significantly differ between the leg and arm. To eliminate diurnal variations, the results were compared with a control group that did not exercise but had blood samples collected at the same time points. In the exercising group, plasma creatine kinase increased progressively during and up to 4 days after exercise. The percentage of CD16+ NK cells increased during exercise, which was paralleled by an increase in the NK cell activity per fixed number of blood mononuclear cells. The NK cell activity on a per NK cell basis did not change. The percentage of CD3+, CD4+, CD8+, CD19+, and CD14+ cells did not change significantly during exercise. The present study thus showed that eccentric exercise with a relatively small muscle mass (1 quadriceps femoris muscle) causes systemic effects on NK cells. It is suggested that the increase in plasma epinephrine during eccentric exercise is responsible for the observed increase in the percentage of CD16+ cells.

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