scholarly journals Immunomodulatory Effects of Perioperative Dexmedetomidine in Ovarian Cancer: An In Vitro and Xenograft Mouse Model Study

2021 ◽  
Vol 11 ◽  
Author(s):  
Seokyung Shin ◽  
Ki Jun Kim ◽  
Hye Jeong Hwang ◽  
Sewon Noh ◽  
Ju Eun Oh ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Ovarian Cancer ◽  
Mouse Model ◽  
Cell Viability ◽  
Nk Cell ◽  
Cell Activity ◽  
Tumor Burden ◽  
Control Group ◽  
Nk Cell Activity ◽  
Dexmedetomidine Infusion

BackgroundThe surgical stress response (SSR) causes immunosuppression which may cause residual tumor growth and micrometastasis after cancer surgery. We investigated whether dexmedetomidine affects cancer cell behavior and immune function in an ovarian cancer xenograft mouse model.MethodsThe effect of dexmedetomidine on cell viability and cell cycle was assessed using SK-OV-3 cells at drug concentrations of 0.5, 0.1, 5, and 10 µg mL-1. BALB/c nude mice were used for the ovarian cancer model with the Dexmedetomidine group (n=6) undergoing surgery with dexmedetomidine infusion and the Control group (n=6) with saline infusion for 4 weeks. Natural killer (NK) cell activity, serum proinflammatory cytokines, and cortisol were measured at predetermined time points and tumor burden was assessed 4 weeks after surgery.ResultsDexmedetomidine had no effect on cell viability or cell cycle. Following a sharp decrease on postoperative day (POD) 1, NK cell activity recovered faster in the Dexmedetomidine group with significant difference vs. the Control group on POD 3 (P=0.028). In the Dexmedetomidine group, cortisol levels were lower on POD 3 (P=0.004) and TNF-α levels were lower at 4 weeks after surgery (P<0.001) compared to the Control group. The Dexmedetomidine group showed lower tumor burden at 4 weeks vs. the Control group as observed by both tumor weight (P<0.001) and the in vivo imaging system (P=0.03).ConclusionsDexmedetomidine infusion may improve ovarian cancer surgery outcome by suppressing the SSR and stress mediator release. Further studies are needed to elucidate the mechanisms by which dexmedetomidine acts on cancer and immune cells.

Depletion of ascorbic acid impairs NK cell activity against ovarian cancer in a mouse model

Immunobiology ◽  
2012 ◽  
Vol 217 (9) ◽  
pp. 873-881 ◽  
Author(s):  
Jee-Eun Kim ◽  
Hang-Sun Cho ◽  
Hoon-Shik Yang ◽  
Da-Jung Jung ◽  
Seung-Woo Hong ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ascorbic Acid ◽  
Mouse Model ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity

Analysis of the Effect of Psychological Nursing Intervention on Perioperative Mood and NK Cell Activity in Breast Cancer Patients

2021 ◽  
Vol 7 (5) ◽  
pp. 3301-3314
Author(s):  
Jihong Yuan ◽  
Linghong Yuan ◽  
Yi Hu ◽  
Jingjing Gong ◽  
Yang Tong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Nk Cell ◽  
Psychological Intervention ◽  
Cell Activity ◽  
Nursing Intervention ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Nk Cell Activity ◽  
Intervention Measures

Breast cancer (Breast Cancer, BC) is one of the most common malignant tumors in women in the world. It is the first malignant tumor that causes the death of women in developing countries. It seriously threatens the lives and health of women and causes damage to the family, economy and society. Through psychological nursing intervention, it has a positive effect on the perioperative mood and NK cell activity of breast cancer patients. The purpose of this article is to explore the effects of psychological intervention on the perioperative mood and NK cell activity of breast cancer patients. This article is based on the concept and theory of psychological nursing intervention for female breast cancer patients, and establishes personalized intervention measures in conjunction with clinical practice to intervene the emotions of breast cancer patients during the perioperative period. This article analyzes the nature, intensity, and causes of negative psychology of the subject through in-depth understanding of the subject’s psychological emotions, coping ability, personality characteristics, past emotional experience, and social support, and discovers the different emotional characteristics of the patient, and formulates the personality psychological intervention measures to stimulate positive and optimistic attitudes and ease the degree of negative psychology. This article analyzes the psychological characteristics of several breast cancer patients. Take corresponding psychological intervention measures. Cope with a series of bad psychology caused by image damage caused by total mastectomy. The results and data in this article show that the incidence of anxiety and depression in the control group is higher than that of the psychological intervention group at 7 days after the operation, with P values of 0.0059 and 0. 0215.Psychological intervention reduces the incidence of negative emotions and reduces the negativeness of patients. The intensity of emotion has played a good clinical effect.

Effect of eccentric exercise on natural killer cell activity

1995 ◽  
Vol 78 (4) ◽  
pp. 1442-1446 ◽  
Author(s):  
J. Palmo ◽  
S. Asp ◽  
J. R. Daugaard ◽  
E. A. Richter ◽  
M. Klokker ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Eccentric Exercise ◽  
Nk Cell ◽  
Killer Cell ◽  
Cell Activity ◽  
Control Group ◽  
Systemic Effects ◽  
Nk Cell Activity ◽  
Blood Samples

The effect of eccentric one-legged exercise on natural killer (NK) cell activity was studied in eight healthy males. To distinguish between local and systemic effects, blood samples were collected from veins in the exercising leg and resting arm. However, the results did not significantly differ between the leg and arm. To eliminate diurnal variations, the results were compared with a control group that did not exercise but had blood samples collected at the same time points. In the exercising group, plasma creatine kinase increased progressively during and up to 4 days after exercise. The percentage of CD16+ NK cells increased during exercise, which was paralleled by an increase in the NK cell activity per fixed number of blood mononuclear cells. The NK cell activity on a per NK cell basis did not change. The percentage of CD3+, CD4+, CD8+, CD19+, and CD14+ cells did not change significantly during exercise. The present study thus showed that eccentric exercise with a relatively small muscle mass (1 quadriceps femoris muscle) causes systemic effects on NK cells. It is suggested that the increase in plasma epinephrine during eccentric exercise is responsible for the observed increase in the percentage of CD16+ cells.

Social Support, Psychological Distress, and Natural Killer Cell Activity in Ovarian Cancer

2005 ◽  
Vol 23 (28) ◽  
pp. 7105-7113 ◽  
Author(s):  
Susan K. Lutgendorf ◽  
Anil K. Sood ◽  
Barrie Anderson ◽  
Stephanie McGinn ◽  
Heena Maiseri ◽  
...  
Keyword(s):  
Social Support ◽  
Ovarian Cancer ◽  
Tumor Microenvironment ◽  
Natural Killer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity ◽  
Nk Cytotoxicity

Purpose Psychosocial stress has been related to impaired immunity in cancer patients. However, the extent to which these relationships exist in immune cells in the tumor microenvironment in humans has not been explored. We examined relationships among distress, social support, and natural killer (NK) cell activity in ovarian cancer patients in peripheral-blood mononuclear cells (PBMC), ascitic fluid, and tumor-infiltrating lymphocytes (TIL). Patients and Methods Patients awaiting surgery for a pelvic mass suspected of being ovarian cancer completed psychological questionnaires and gave a presurgical sample of peripheral blood. Samples of tumor and ascites were taken during surgery, lymphocytes were then isolated, and NK cytotoxicity and percentage were determined. The final sample, which was confirmed by surgical diagnosis, included 42 patients with epithelial ovarian cancer and 23 patients with benign masses. Results Peripheral NK cell activity was significantly lower among ovarian cancer patients than in patients with benign masses. Among ovarian cancer patients, NK cytotoxicity in TIL was significantly lower than in PBMC or ascitic fluid. Social support was related to higher NK cytotoxicity in PBMC and TIL, adjusting for stage. Distress was related to lower NK cytotoxicity in TIL. A multivariate model indicated independent associations of both distress and social support with NK cell activity in TIL. Conclusion Psychosocial factors, such as social support and distress, are associated with changes in the cellular immune response, not only in peripheral blood, but also at the tumor level. These relationships were more robust in TIL. These findings support the presence of stress influences in the tumor microenvironment.

Analysis of the Effect of Psychological Nursing Intervention on Perioperative Mood and NK Cell Activity in Breast Cancer Patients

2021 ◽  
Vol 7 (4) ◽  
pp. 443-456
Author(s):  
Jihong Yuan ◽  
Linghong Yuan ◽  
Yi Hu ◽  
Jingjing Gong ◽  
Yang Tong ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Nk Cell ◽  
Psychological Intervention ◽  
Cell Activity ◽  
Nursing Intervention ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Nk Cell Activity ◽  
Intervention Measures

Breast cancer (Breast Cancer, BC) is one of the most common malignant tumors in women in the world. It is the first malignant tumor that causes the death of women in developing countries. It seriously threatens the lives and health of women and causes damage to the family, economy and society. Through psychological nursing intervention, it has a positive effect on the perioperative mood and NK cell activity of breast cancer patients. The purpose of this article is to explore the effects of psychological intervention on the perioperative mood and NK cell activity of breast cancer patients. This article is based on the concept and theory of psychological nursing intervention for female breast cancer patients, and establishes personalized intervention measures in conjunction with clinical practice to intervene the emotions of breast cancer patients during the perioperative period. This article analyzes the nature, intensity, and causes of negative psychology of the subject through in - depth understanding of the subject's psychological emotions, coping ability, personality characteristics, past emotional experience, and social support, and discovers the different emotional characteristics of the patient, and formulates the personality psychological intervention measures to stimulate positive and optimistic attitudes and ease the degree of negative psychology. This article analyzes the psychological characteristics of several breast cancer patients. Take corresponding psychological intervention measures. Cope with a series of bad psychology caused by image damage caused by total mastectomy. The results and data in this article show that the incidence of anxiety and depression in the control group is higher than that of the psychological intervention group at 7 days after the operation, with P values of 0.0059 and 0. 0215. Psychological intervention reduces the incidence of negative emotions and reduces the negativeness of patients. The intensity of emotion has played a good clinical effect.

scholarly journals Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Myoung Hwa Kim ◽  
Jeong-Rim Lee ◽  
Ki-Joon Kim ◽  
Ji Hae Jun ◽  
Hye Jeong Hwang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nk Cell ◽  
Cell Activity ◽  
Treated Group ◽  
Control Group ◽  
Nk Cell Activity ◽  
Clinical Dose ◽  
Group 2 ◽  
Mcf 7 ◽  
Group 1

AbstractIn our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the anti-tumor effect of tramadol in vivo. Female nude mice orthotopically inoculated with luciferase-expressing MCF-7 cells, were randomly divided into the control (saline), tramadol group 1 (1.5 mg kg−1 day−1), tramadol group 2 (3 mg kg−1 day−1), and morphine (0.5 mg kg−1 day−1) (n = 5/group). Bioluminescence signals after D-luciferin injection, tumor size, and tumor weight were compared among groups after 4 weeks. Estrogen receptor (ER), progesterone receptor (PR), and transient receptor potential vanilloid (TRPV)-1 expression, natural killer (NK) cell activity, and serum interleukin (IL)-1β, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-6 were then examined. Tumour growth was attenuated in tramadol-treated groups (P < 0.05). NK cell activity was significantly decreased only in the morphine treated group not in sham, control, and tramadol groups. The expression levels of ERα, PRα and β, and TRPV1 were decreased in tramadol group 2 compared with those in the morphine group, but not compared to the control group. Serum levels of IL-6 and TNFα were reduced in both tramadol-treated group 1 and 2 compared to the control group. Overall, clinical dose of tramadol has anti-tumour effects on MCF-7 cell-derived breast cancer in a xenograft mouse model.

scholarly journals The effect of surgery and chemotherapy on blood NK cell activity in patients with ovarian cancer

Cancer ◽  
1983 ◽  
Vol 51 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Barbara Lukomska ◽  
Waldemar L. Olszewski ◽  
Arnfinn Engeset ◽  
Per Kolstad
Keyword(s):  
Ovarian Cancer ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity

scholarly journals Inhibition of NK cell activity by IL-17 allows vaccinia virus to induce severe skin lesions in a mouse model of eczema vaccinatum

2009 ◽  
Vol 206 (6) ◽  
pp. 1219-1225 ◽  
Author(s):  
Yuko Kawakami ◽  
Yoshiaki Tomimori ◽  
Kenji Yumoto ◽  
Shunji Hasegawa ◽  
Tomoaki Ando ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Vaccinia Virus ◽  
Nk Cells ◽  
Nk Cell ◽  
Cytotoxic T Lymphocyte ◽  
Cell Activity ◽  
Skin Lesions ◽  
Smallpox Vaccination ◽  
Nk Cell Activity

Threats of bioterrorism have renewed efforts to better understand poxvirus pathogenesis and to develop a safer vaccine against smallpox. Individuals with atopic dermatitis are excluded from smallpox vaccination because of their propensity to develop eczema vaccinatum, a disseminated vaccinia virus (VACV) infection. To study the underlying mechanism of the vulnerability of atopic dermatitis patients to VACV infection, we developed a mouse model of eczema vaccinatum. Virus infection of eczematous skin induced severe primary erosive skin lesions, but not in the skin of healthy mice. Eczematous mice exhibited lower natural killer (NK) cell activity but similar cytotoxic T lymphocyte activity and humoral immune responses. The role of NK cells in controlling VACV-induced skin lesions was demonstrated by experiments depleting or transferring NK cells. The proinflammatory cytokine interleukin (IL)-17 reduced NK cell activity in mice with preexisting dermatitis. Given low NK cell activities and increased IL-17 expression in atopic dermatitis patients, these results can explain the increased susceptibility of atopic dermatitis patients to eczema vaccinatum.

Immune Responses to Guided Imagery During Breast Cancer Treatment

2008 ◽  
Vol 9 (3) ◽  
pp. 205-214 ◽  
Author(s):  
Cecile A. Lengacher ◽  
Mary P. Bennett ◽  
Lois Gonzalez ◽  
Danielle Gilvary ◽  
Charles E. Cox ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nk Cells ◽  
Guided Imagery ◽  
Nk Cell ◽  
Intervention Group ◽  
Cell Activity ◽  
Control Group ◽  
Cell Cytotoxicity ◽  
Nk Cell Activity ◽  
Nk Cell Cytotoxicity

Background: The use of relaxation and guided imagery to reduce stress and improve immune function has great potential benefits for patients with breast cancer. Methods: This pilot study used a pretest—posttest experimental design with 28 breast cancer patients, aged 25 to 75 years, with the diagnosis of stage 0, 1, or 2 breast cancer. The experimental group received a relaxation and guided imagery intervention and the control group received standard care. The effects of the intervention on immune function were measured by natural killer (NK) cell cytotoxicity and IL-2—activated NK cell activity prior to surgery and 4 weeks postsurgery. NK cell activity was measured using a 15-hr incubation chromium release assay. Cytotoxicity of NK cells was measured against chromium-labeled K-562 target cells. IL-2 was used to enhance reactivity of NK cells against tumor cells. After incubation for 15 hr, cytotoxicity was measured through the release of radioactive chromium. Results: Significant differences between groups were found at 4 weeks postsurgery. T-tests showed increased NK cell cytotoxicity for the intervention group at 100:1, 50:1, and 25:1 effector cell: target cell ratios (E:T) ( p < .01 to p < .05) and increased activation for IL-2 at 100:1, 50:1, 25:1, and 12.5:1 (E:T) ( p < .01 to p < .05) for the intervention group as compared to the control group. Discussion: These findings suggest that a relaxation intervention such as guided imagery could have an effect on NK cell cytotoxicity and NK cell cytotoxicity after activation with IL-2 in patients undergoing surgery for breast cancer.

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