scholarly journals Morphological reassessment of the movable calcar of delphacid planthoppers (Hemiptera: Fulgoromorpha: Delphacidae)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Darya Markevich ◽  
Marcin Walczak ◽  
Oleg Borodin ◽  
Jacek Szwedo ◽  
Jolanta Brożek

AbstractThis study presents the morphology of calcar in adult Delphacidae based on representatives of the genera Ugyops Guérin-Meneville, 1834, Notuchus Fennah, 1969 (Ugyopini), Asiraca Latreille, 1798 (Asiracini), Kelisia Fieber, 1866, (Kelisini), Stenocranus Fieber, 1866 (Stenocranini), Chloriona Fieber, 1866, Megadelphax Wagner, 1963, Muellerianella Wagner, 1963, Javesella Fennah, 1963, Conomelus Fieber, 1866, Euconomelus Haupt, 1929, Hyledelphax Vilbaste, 1968, Stiroma Fieber, 1866, Struebingianella Wagner, 1963 and Xanthodelphax Wagner, 1963 (Delphacini). We used SEM electron microscopy, to define seven types of calcar structure (Types 1, 2, 5, 6, 7, 8, and 9) based on combinations of characters including shape, number of teeth and differentiation of sensory structures in species from fifteen genera. Additionally, two other types (Types 3 and 4) were determined based on the calcar descriptions from previous studies. Similarities and differences in calcar structure and function were discussed and emerging relationships between planthopper species and their particular habitats were indicated.

Author(s):  
Robert L. Ochs

By conventional electron microscopy, the formed elements of the nuclear interior include the nucleolus, chromatin, interchromatin granules, perichromatin granules, perichromatin fibrils, and various types of nuclear bodies (Figs. 1a-c). Of these structures, all have been reasonably well characterized structurally and functionally except for nuclear bodies. The most common types of nuclear bodies are simple nuclear bodies and coiled bodies (Figs. 1a,c). Since nuclear bodies are small in size (0.2-1.0 μm in diameter) and infrequent in number, they are often overlooked or simply not observed in any random thin section. The rat liver hepatocyte in Fig. 1b is a case in point. Historically, nuclear bodies are more prominent in hyperactive cells, they often occur in proximity to nucleoli (Fig. 1c), and sometimes they are observed to “bud off” from the nucleolar surface.


1999 ◽  
Vol 82 (08) ◽  
pp. 271-276 ◽  
Author(s):  
Glen Spraggon ◽  
Stephen Everse ◽  
Russell Doolittle

IntroductionAfter a long period of anticipation,1 the last two years have witnessed the first high-resolution x-ray structures of fragments from fibrinogen and fibrin.2-7 The results confirmed many aspects of fibrinogen structure and function that had previously been inferred from electron microscopy and biochemistry and revealed some unexpected features. Several matters have remained stubbornly unsettled, however, and much more work remains to be done. Here, we review several of the most significant findings that have accompanied the new x-ray structures and discuss some of the problems of the fibrinogen-fibrin conversion that remain unresolved. * Abbreviations: GPR—Gly-Pro-Arg-derivatives; GPRPam—Gly-Pro-Arg-Pro-amide; GHRPam—Gly-His-Arg-Pro-amide


1998 ◽  
Vol 79 (05) ◽  
pp. 1034-1040 ◽  
Author(s):  
E. Nitschmann ◽  
L. Berry ◽  
S. Bridge ◽  
M. W. C. Hatton ◽  
M. Richardson ◽  
...  

SummaryWe hypothesised that there are important physiologic differences in arterial wall structure and function with respect to antithrombotic activity in the very young (pre-puberty) compared to adults. Electron microscopy, gel electrophoresis, and activity assays were used to examine differences in aorta structure and function comparing prepubertal rabbits (pups) to adult rabbits. Differences in endothelial function, extracellular matrix structure, proteoglycan (PG) distribution and glycosaminoglycan (GAG) content and function were shown. In both intima and media, total PG, chondroitin sulfate (CS) PG and heparan sulfate (HS) PG content were significantly increased in pups compared to adult rabbits. These findings corresponded to increased concentrations by mass analyses of CS GAG and DS GAG in aortas from pups. There was also a significant increase in antithrombin activity in pups due to HS GAG. In conclusion, differences in both structure and antithrombin activity of aortas from pups compared to adult rabbits suggest that young arteries may have greater antithrombotic potential that is, at least in part, related to increased HS GAG.


1999 ◽  
Vol 232 (1-2) ◽  
pp. 169-178 ◽  
Author(s):  
John M Robinson ◽  
Toshihiro Kobayashi ◽  
Harumichi Seguchi ◽  
Toshihiro Takizawa

2020 ◽  
Vol 8 (1) ◽  
pp. 485-496 ◽  
Author(s):  
Vikas Raj Sharma ◽  
Ananya Shrivastava ◽  
Benoit Gallet ◽  
Elizaveta Karepina ◽  
Peggy Charbonnier ◽  
...  

The combined use of light sheet fluorescence microscopy and 3D electron microscopy enables to reveal the fine details of bile canaliculi structure and function in matrix-free hepatic spheroids.


1990 ◽  
Vol 259 (4) ◽  
pp. G564-G570 ◽  
Author(s):  
S. Arvidsson ◽  
K. Carter ◽  
A. Yanaka ◽  
S. Ito ◽  
W. Silen

The effects of intracellular acidosis induced by acidification of the basolateral (nutrient) perfusate on the structure and function of the oxynticopeptic cell were studied in in vitro frog gastric mucosa. Changing the pH of the unbuffered nutrient perfusate (UNB) from 7.2 to 3.5 acidified the oxynticopeptic cell with no change in potential difference (PD) or resistance (R). Intracellular pH (pHi), PD, and R were 7.05 +/- 0.01, 16 +/- 1 mV, 165 +/- 7 omega.cm2 before and 6.44 +/- 0.01, 16 +/- 2 mV, 170 +/- 9 omega.cm2 after nutrient acidification. Acid secretion (H+) increased from 0.86 +/- 0.07 to 1.88 +/- 0.18 mu eq.cm-2.h-1. Addition of forskolin to tissues perfused with nutrient pH (pHn) 3.5 decreased PD to 2 +/- 2 mV and further increased H+ to 3.07 +/- 0.19 mu eq.cm-2.h-1. By light and electron microscopy oxynticopeptic cells perfused with UNB, pHn 3.5, appeared normal. Oxynticopeptic cells in tissues pretreated with omeprazole and then exposed to UNB, pHn 3.5, had extensive morphological damage. On increasing the pH of the nutrient perfusate from 3.5 to 7.2 there was prompt recovery of pHi in untreated and forskolin-stimulated mucosae (pHi 6.87 +/- 0.06 and 6.85 +/- 0.04) but no recovery of pHi in tissues pretreated with omeprazole or cimetidine (pHi 6.26 +/- 0.04 and 6.44 +/- 0.06, n = 6, 30 min after reexposure to UNB, pHn 7.2). We conclude that in a secreting mucosa intracellular acidification of the oxynticopeptic cell to pHi 6.4 is associated with normal morphology, PD, R, and increased H+, and that intracellular acidosis is not de facto deleterious.


2011 ◽  
Vol 17 (S2) ◽  
pp. 226-227
Author(s):  
J Timlin ◽  
A Collins ◽  
M Liberton ◽  
H Jones ◽  
H Pakrasi

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.


Author(s):  
A.G. Lewis ◽  
L. Chatters ◽  
M. Raudsepp

Tigriopus californicus uses several appendages and processes in the collection and manipulation of food. Their structure and function appear to enable the species to utilize the variety of food materials found in splashpools, including: detritus and organic floes; superficial material on particles; faecal pellets; protists; diatoms; and small crustaceans. From light and scanning electron microscopy and video, the labrum and labium appear to be adapted for biting soft and hard food materials and holding food for trituration by the mandibles. From energy dispersive X-ray spectra, the gnathobase of the mandible is suggested to be sclerotized but not calcified or silicified. It has an array of bilobate and multilobate teeth, clusters of spinous processes, and a heavy, spine-bearing process to move food into the oesophagus. There is also a flange which articulates in a groove in the labrum which appears to provide a guide for the gnathobase as it moves vertically.


1983 ◽  
Vol 13 (2) ◽  
pp. 255-266 ◽  
Author(s):  
P. L. Lantos

SYNOPSISOur concept of the structure and function of the normal and diseased brain has developed throughout the centuries. The first stage in the discovery of the brain stretched over three millennia, from the earliest descriptions by the Egyptians in the sixteenth century B.C. to the comprehensive anatomical treatise of Vesalius. The invention of the light microscope brought to the eye a previously invisible world, and heralded the beginnings of the systematic histological investigation of the immensely complex cellular networks of the brain. With the advent of electron microscopy, the organelles and connections of brain cells have been revealed, and the new era of molecular biology has begun. Neuropathology, which concerns itself with diseases of the brain, spinal cord, nerve and muscle, has enormously benefited from these developments to establish the morphological basis of diseases of the nervous system.


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