Author Correction: Catechol thwarts virulent dimorphism in Candida albicans and potentiates the antifungal efficacy of azoles and polyenes

Background: Fluconazole (FLZ), a potent antifungal medication, is characterized by poor water solubility that reduced its antifungal efficacy. Objective: This study aimed to prepare FLZ-loaded polymeric nanoparticles (NPs) by using different polymers and techniques as a mean of enhancing the antifungal activity of FLZ. Methods: NP1, NP2, and NP3 were prepared by the double emulsion/solvent evaporation method using PLGA, PCL, and PLA, respectively. The ionotropic pre-gelation technique was applied to prepare an alginate/chitosan-based formulation (NP4). Particle size, zeta potential, encapsulation efficiency, and loading capacity were characterized. FT-IR spectra of FLZ, the polymers, and the prepared NPs were estimated. NP4 was selected for further in-vitro release evaluation. The broth dilution method was used to assess the antifungal activity of NP4 using a resistant clinical isolate of Candida albicans. Results: The double emulsion method produced smaller-sized particles (<390 nm) but with much lower encapsulation efficiency (< 12%). Alternatively, the ionic gelation method resulted in nanosized particles with a markedly higher encapsulation efficiency of about 40%. The FT-IR spectroscopy confirmed the loading of the FLZ molecules in the polymeric network of the prepared NPs. The release profile of NP4 showed a burst initial release followed by a controlled pattern up to 24 hours with a higher percent released relative to the free FLZ suspension. NP4 was able to reduce the value of MIC of FLZ by 20 times. Conclusion: The antifungal activity of FLZ against C. albicans was enhanced markedly via its loading in the alginate/chitosan-based polymeric matrix of NP4.

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In vitro comparative evaluation of antifungal efficacy of three endodontic sealers with and without incorporation of chitosan nanoparticles against Candida albicans

Journal of Conservative Dentistry ◽

10.4103/jcd.jcd_242_19 ◽

2019 ◽

Vol 22 (6) ◽

pp. 564

Author(s):

Amit Jena ◽

Satabdi Pattanaik ◽

Govind Shashirekha

Keyword(s):

Candida Albicans ◽

Comparative Evaluation ◽

Chitosan Nanoparticles ◽

Endodontic Sealers ◽

Antifungal Efficacy

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ANTIFUNGAL EFFICACY OF SECANG HEARTWOOD (CAESALPINIA SAPPAN L.) SOLUTIONS ON BIOFILMS OF CANDIDA ALBICANS ATCC 10231

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s1.16060 ◽

2019 ◽

pp. 160-163

Author(s):

BRINNA LISTIANI ◽

RATNA MEIDYAWATI ◽

DEWA AYU NPA ◽

DWI ARNIAWATY

Keyword(s):

Candida Albicans ◽

Root Canal ◽

Fungal Species ◽

Mean Value ◽

Control Group ◽

Caesalpinia Sappan ◽

Irrigation Solution ◽

The Mean ◽

Preliminary Study ◽

Antifungal Efficacy

Objective: Candida albicans is a predominant fungal species found in persistent root canal infection, and its virulence depends on the ability to formbiofilms. To be able to eliminate this infection, a safe irrigation solution during root canal preparation is needed. This study is conducted to investigatethe antifungal properties of secang heartwood extract solutions on C. albicans biofilm.Methods: C. albicans biofilm was obtained from incubation of C. albicans ATCC 10231 at 96-well plates in 37°C for 24 h. These biofilms were exposedto various treatments: Secang heartwood in three different concentrations (25%, 33%, and 50%), sodium hypochlorite (NaOCl) 2.5%, and controlgroup. The results of the treatment are to see the optical density (OD) value. The higher the OD values, the lower antifungal activity of the solution.Results: The mean OD result from six samples obtained the mean value of various OD values showed in ELISA reader. Antifungal efficacy of 25%secang heartwood was lower than concentration of 33% and 50%. All concentrations of secang heartwood showed higher OD values than 2.5% NaOClbut lower than control group with p=0.01.Conclusion: Secang heartwood possesses antifungal effects against C. albicans biofilm but is lower than 2.5% NaOCl. Concentration of 25% has thestrongest effect against C. albicans among other concentrations. This was a preliminary study of the antifungal efficacy of secang heartwood extractsolutions against C. albicans biofilm. The result indicates that this solution has the potential to be used as an herbal alternative in root canal irrigations.

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Development and Optimization of Luliconazole Spanlastics to Augment the Antifungal Activity against Candida albicans

Pharmaceutics ◽

10.3390/pharmaceutics13070977 ◽

2021 ◽

Vol 13 (7) ◽

pp. 977

Author(s):

Nabil A. Alhakamy ◽

Mohammed W. Al-Rabia ◽

Shadab Md ◽

Alaa Sirwi ◽

Selwan Saud Khayat ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Skin Penetration ◽

Complete Removal ◽

Process Variable ◽

Skin Infections ◽

Sonication Time ◽

Swiss Mice ◽

Low Solubility ◽

Antifungal Efficacy

Luliconazole is a new topical imidazole antifungal drug for the treatment of skin infections. It has low solubility and poor skin penetration which limits its therapeutic applications. In order to improve its therapeutic efficacy, spanlastics nanoformulation was developed and optimized using a combined mixture-process variable design (CMPV). The optimized formulation was converted into a hydrogel formula to enhance skin penetration and increase the efficacy in experimental cutaneous Candida albicans infections in Swiss mice wounds. The optimized formulation was generated at percentages of Span and Tween of 48% and 52%, respectively, and a sonication time of 6.6 min. The software predicted that the proposed formulation would achieve a particle size of 50 nm with a desirability of 0.997. The entrapment of luliconazole within the spanlastics carrier showed significant (p < 0.0001) antifungal efficacy in the immunocompromised Candida-infected Swiss mice without causing any irritation, when compared to the luliconazole treated groups. The microscopic observation showed almost complete removal of the fungal colonies on the skin of the infected animals (0.2 ± 0.05 log CFU), whereas the control animals had 0.2 ± 0.05 log CFU. Therefore, luliconazole spanlastics could be an effective formulation with improved topical delivery for antifungal activity against C. albicans.

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Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001

10.1101/577817 ◽

2019 ◽

Author(s):

Sagarika Devi ◽

Heiko T. Kiesewalter ◽

Renátó Kovács ◽

Jens Christian Frisvad ◽

Tilmann Weber ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Biofilm Formation ◽

Pathogenic Fungi ◽

Plant Pathogenic Fungi ◽

Bacillus Velezensis ◽

High Demand ◽

Sustainable Environment ◽

Inhibitory Potential ◽

Antifungal Efficacy

ABSTRACTFor a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.

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