scholarly journals Postoperative proliferative vitreoretinopathy development is linked to vitreal CXCL5 concentrations

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Souska Zandi ◽  
Isabel B. Pfister ◽  
Justus G. Garweg
Keyword(s):  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
De Novo ◽  
Multiple Comparisons ◽  
Vitreous Fluid ◽  
Gm Csf ◽  
Cytokines And Chemokines ◽  
Pathophysiological Role ◽  
Statistical Level ◽  
Ifn Γ

AbstractThe specific changes linked to de novo development of postoperative PVR have remained elusive and were the object of the underlying study. Vitreous fluid (VF) was obtained at the beginning of vitrectomy from 65 eyes that underwent vitrectomy for primary rhegmatogenous retinal detachment (RRD) without preoperative PVR. Eyes developing postoperative PVR within 6 months after re-attachment surgery were compared to those which did not regarding the preoperative concentrations of 43 cytokines and chemokines in the VF, using multiplex beads analysis. For all comparisons Holm’s correction was applied in order to control for multiple comparisons. Twelve out of 65 eyes (18.5%) developed PVR postoperatively. While 12 of the chemokines and cytokines presented concentration differences on a statistical level of p < 0.05 (CXCL5, CCL11, CCL24, CCL26, GM-CSF, IFN-γ, CCL8, CCL7, MIF, MIG/CXCL9, CCL19, and CCL25), CXCL5 was the only cytokine with sufficiently robust difference in its VF concentrations to achieve significance in eyes developing postoperative PVR compared to eyes without PVR. CXCL5 may represent a potent biomarker for the de novo development of postoperative PVR. In line with its pathophysiological role in the development of PVR, it might serve as a basis for the development of urgently needed preventive options.

scholarly journals IMMMUNE MEDIATORS IN IDIOPATHIC UVEITIS

2021 ◽  
Author(s):  
Marie-Helene Errera ◽  
Ana Pratas ◽  
Sylvain Fisson ◽  
Thomas Manicom ◽  
Marouane Boubaya ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Unsupervised Classification ◽  
Control Group ◽  
Serum Samples ◽  
Gm Csf ◽  
Cytokines And Chemokines ◽  
Age Related ◽  
Order Of Magnitude ◽  
Ifn Γ ◽  
Endothelial Growth Factor

To investigate which cytokines, chemokines and growth factors are involved in the immunopathogenesis of idiopathic uveitis, and whether cytokine profiles are associated with. Serum and aqueous humor (AH) samples of 75 patients with idiopathic uveitis were analyzed by multiplex immunoassay. Infectious controls consisted of 16 patients with ocular toxoplasmosis all confirmed by intraocular fluid analyses. Noninfectious controls consisted of 7 patients with Behçet disease related uveitis and 15 patients with sarcoidosis related uveitis. The control group consisted of AH and serum samples from 47 noninflammatory control patients with age-related cataract. In each sample, 27 immune mediators ± IL-21 and IL-23 were measured. In idiopathic uveitis, 13 of the 29 mediators, including most proinflammatory and vascular mediators such as IL-6, IL-8, IL-12, G-CSF, GM-CSF, MCP-1, IP-10, TNF-α and VEGF, were significantly elevated in the aqueous humor when compared to all controls. Moreover, IL-17, IP-10, and IL-21, were significantly elevated in the serum when compared to all controls. We clustered 4 subgroups of idiopathic uveitis using a statistical analysis of hierarchical unsupervised classification, characterized by the order of magnitude of concentrations of intraocular cytokines. The pathogenesis of idiopathic uveitis is characterized by the presence of predominantly proinflammatory cytokines and chemokines and vascular endothelial growth factor with high expression levels as compared to other causes of uveitis. There are indications for obvious Th-1/ IL21-Th17 pathways but also IL9-Th9 and increased IFN-γ-inducing cytokine (IL12) and IFN-γ-inducible CXC chemokine (IP-10). The combined data suggest that immune mediator expression is different among idiopathic uveitis. This study suggests various clusters among the idiopathic uveitis group rather than one specific uveitis entity.

scholarly journals Extracellular purine metabolism and signaling of CD73-derived adenosine in murine Treg and Teff cells

2011 ◽  
Vol 301 (2) ◽  
pp. C530-C539 ◽  
Author(s):  
Michael Romio ◽  
Benjamin Reinbeck ◽  
Sabine Bongardt ◽  
Sandra Hüls ◽  
Sandra Burghoff ◽  
...  
Keyword(s):  
T Cells ◽  
Proinflammatory Cytokines ◽  
Wild Type ◽  
Murine Splenocytes ◽  
Gm Csf ◽  
A2a Receptors ◽  
Cytokines And Chemokines ◽  
Tnf Α ◽  
Ifn Γ ◽  
Macrophage Colony

CD73-derived adenosine acts as potent inhibitor of inflammation, and regulatory T cells (Treg) have been shown to express CD73 as a novel marker. This study explored the role of endogenously formed adenosine in modulating NF-κB activity and cytokine/chemokine release from murine Treg and effector T cells (Teff) including key enzymes/purinergic receptors of extracellular ATP catabolism. Stimulating murine splenocytes and CD4+ T cells with anti-CD3/anti-CD28 significantly upregulated activated NF-κB in CD73−/− T cells (wild type: 4.36 ± 0.21; CD73−/−: 6.58 ± 0.75; n = 4; P = 0.029). This was associated with an augmented release of proinflammatory cytokines IL-2, TNF-α, and IFN-γ. Similar changes were observed with the CD73 inhibitor APCP (50 μM) on NF-κB and IFN-γ in wild-type CD4+ T-cells. Treatment of stimulated CD4+ T-cells with adenosine (25 μM) potently reduced IFN-γ release which is mediated by adenosine A2a receptors (A2aR). AMP (50 μM) also reduced cytokine release which was not inhibited by APCP. In Teff, A2aR activation (CGS21680) potently inhibited the release of IL-1, IL-2, IL-3, IL-4, IL-12, IL-13, IFN-γ, TNF-α, granulocyte-macrophage colony-stimulating factor (GM-CSF), CCL3, and CCL4. However, in Treg, CGS21680 did not alter cytokine/chemokine release. In summary, CD73-derived adenosine tonically inhibits active NF-κB in CD4+ T-cells, thereby modulating the release of a broad spectrum of proinflammatory cytokines and chemokines. Downregulation of P2X7 and upregulation of CD73 in Treg after antigenic stimulation may be an important mechanism to maintain the ability of Treg to generate immunosuppressive adenosine.

scholarly journals Oxidative Stress in the Vitreous Fluid with Rhegmatogenous Retinal Detachment

2017 ◽  
Vol 08 (05) ◽  
Author(s):  
Shizuka Takahashi ◽  
Atsuko Maeno ◽  
Mitsuru Nakazawa ◽  
Yukihiko Suzuki ◽  
Kobu Adachi
Keyword(s):  
Oxidative Stress ◽  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Vitreous Fluid

scholarly journals Antioxidant Status in the Vitreous of Eyes with Rhegmatogenous Retinal Detachment with and without Proliferative Vitreoretinopathy, Macular Hole and Epiretinal Membrane

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 453
Author(s):  
Agata Pietras-Baczewska ◽  
Katarzyna Nowomiejska ◽  
Agnieszka Brzozowska ◽  
Mario Damiano Toro ◽  
Wojciech Załuska ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Macular Hole ◽  
Epiretinal Membrane ◽  
Proliferative Vitreoretinopathy ◽  
Antioxidant Status ◽  
Rhegmatogenous Retinal Detachment ◽  
Vitreous Body ◽  
Total Antioxidant Status ◽  
Antioxidant Enzyme Activities ◽  
Vitreous Fluid

(1) Background: The aim of the study was to test the hypothesis that the antioxidant status in the vitreous body of eyes, which had been vitrectomized due to rhegmatogenous retinal detachment (RRD) with or without proliferative vitreoretinopathy (PVR), is higher than in eyes vitrectomized due to other retinal diseases. (2) Methods: four patient groups were analyzed: 22 eyes of patients with RRD without PVR, 27 eyes with RRD and PVR, 22 eyes with macular hole (MH) and 10 eyes with epiretinal membrane (ERM). Spectrophotometric methods were used to determine the total antioxidant status (TAS) values as well as superoxide dismutase (SOD) and glutathione reductase (GR) activities in the vitreous fluid samples. (3) Results: no significant differences in TAS values and antioxidant enzyme activities were observed among patient with RRD with and without PVR and with MH and ERM. The longer the duration of RRD leading to PVR and better postoperative visual acuity, the higher the TAS level. No significant differences were found between “macula on” and “macula off” subgroups within the RRD group and the RRD combined with PVR group. (4) Conclusions: The preliminary results do not support the thesis that the antioxidant status of vitrectomized eyes is different in patients with RRD with or without PVR in comparison to patients with MH and ERM. In patients with RRD, PVR presence and detached macula do not affect the values of TAS, SOD and GR in the vitreous fluid. The duration of the disease influences TAS in the vitreous in eyes with RRD complicated with PVR.

scholarly journals Characterization of the biological antioxidant potential in the vitreous fluid from patients with rhegmatogenous retinal detachment

2016 ◽  
Vol 94 (6) ◽  
Author(s):  
Atsuko Maeno ◽  
Yukihiko Suzuki ◽  
Kobu Adachi ◽  
Shizuka Takahashi ◽  
Yumiko Yokoi ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Antioxidant Potential ◽  
Vitreous Fluid

scholarly journals Profiles of Inflammatory Cytokines in the Vitreous Fluid from Patients with Rhegmatogenous Retinal Detachment and Their Correlations with Clinical Features

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Shizuka Takahashi ◽  
Kobu Adachi ◽  
Yukihiko Suzuki ◽  
Atsuko Maeno ◽  
Mitsuru Nakazawa
Keyword(s):  
Diabetic Retinopathy ◽  
Retinal Detachment ◽  
Clinical Features ◽  
Inflammatory Cytokines ◽  
Rhegmatogenous Retinal Detachment ◽  
Vitreous Fluid ◽  
Vein Occlusion ◽  
Pathologic Features ◽  
Vitreoretinal Diseases ◽  
Photoreceptor Damage

Purpose. To characterize the profiles for inflammatory cytokines in the vitreous fluid from patients with rhegmatogenous retinal detachment (RRD) by comparing those of other vitreoretinal diseases and to analyze the correlation between intravitreal cytokines and clinical features.Materials and Methods. Vitreous fluid was obtained at the time of surgery from 28 RRD eyes. Vitreous fluid was similarly collected from patients with macular hole (MH), epiretinal membrane, proliferative diabetic retinopathy (PDR), and retinal vein occlusion as controls. Twenty-seven cytokines were measured. Intravitreal cytokine profiles in RRD were characterized by comparing these with those in other vitreoretinal diseases. We also analyzed the correlations between vitreous cytokines and clinical features.Results. There were statistical differences in the MCP-1, MIP-1β, and IP-10 between the RRD and MH, while the IL-6 and IL-8 exhibited levels that were between those for the PDR and MH. MIP-1βwas significantly correlated to both the extent and duration of the RRD, while IL-8 was significantly correlated to the extent of the RRD.Conclusions. MCP-1, MIP-1β, and IP-10 may modify the pathologic features of RRD. The levels of these cytokines are related in part to the clinical features and the level of photoreceptor damage.

scholarly journals Correlation between miR-148 Expression in Vitreous and Severity of Rhegmatogenous Retinal Detachment

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Taichi Tsunekawa ◽  
Hiroki Kaneko ◽  
Kei Takayama ◽  
Shiang-Jyi Hwang ◽  
Akio Oishi ◽  
...  
Keyword(s):  
Retinal Detachment ◽  
Rhegmatogenous Retinal Detachment ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Vitreous Hemorrhage ◽  
Retinal Break ◽  
Vitreous Fluid ◽  
Mesenchymal Transition ◽  
Expression Levels ◽  
Retinal Breaks

Purpose. We had earlier reported positive hsa-miR-148a-3p expression in eyes with rhegmatogenous retinal detachment (RRD) and its involvement in the epithelial-mesenchymal transition of retinal pigment epithelium in vitro. Here we investigated the association of hsa-miR-148a-3p expression levels in the vitreous fluid of patients with RRD with severity of RRD. Methods. The hsa-miR-148a-3p expression levels in the vitreous fluid, range (degree) of retinal detachment (RD), and pixels of retinal break were measured in 27 eyes with RRD. The association of hsa-miR-148a-3p expression levels with other factors was evaluated by multiple regression analysis. Results. The hsa-miR-148a-3p expression levels, time from onset of RRD to vitrectomy, range of RD, and pixels of retinal breaks were 23.68±43.00, 12.07±15.36 days, 155.85±86.67 degrees, and 37000±67100 pixels, respectively. Five eyes with RRD had vitreous hemorrhage preoperatively. The hsa-miR-148a-3p expression levels were significantly associated with pixels of retinal breaks (β=0.699) and the time from onset of RRD to vitrectomy (β=0.358) but not with the range of RD or presence of vitreous hemorrhage. Conclusion. The hsa-miR-148a-3p expression levels in the vitreous fluid were significantly associated with the size of retinal break and disease duration.

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