scholarly journals Measurement of hindered diffusion in complex geometries for high-speed studies of single-molecule forces

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tobias F. Bartsch ◽  
Camila M. Villasante ◽  
Felicitas E. Hengel ◽  
Ahmed Touré ◽  
Daniel M. Firester ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
High Speed ◽  
Optical Trap ◽  
Glass Coverslip ◽  
Hydrodynamic Coupling ◽  
Hindered Diffusion ◽  
Protocadherin 15 ◽  
Human Hearing ◽  
Infinite Wall

AbstractIn a high-speed single-molecule experiment with a force probe, a protein is tethered between two substrates that are manipulated to exert force on the system. To avoid nonspecific interactions between the protein and nearby substrates, the protein is usually attached to the substrates through long, flexible linkers. This approach precludes measurements of mechanical properties with high spatial and temporal resolution, for rapidly exerted forces are dissipated into the linkers. Because mammalian hearing operates at frequencies reaching tens to hundreds of kilohertz, the mechanical processes that occur during transduction are of very short duration. Single-molecule experiments on the relevant proteins therefore cannot involve long tethers. We previously characterized the mechanical properties of protocadherin 15 (PCDH15), a protein essential for human hearing, by tethering an individual monomer through very short linkers between a probe bead held in an optical trap and a pedestal bead immobilized on a glass coverslip. Because the two confining surfaces were separated by only the length of the tethered protein, hydrodynamic coupling between those surfaces complicated the interpretation of the data. To facilitate our experiments, we characterize here the anisotropic and position-dependent diffusion coefficient of a probe in the presence of an effectively infinite wall, the coverslip, and of the immobile pedestal.

scholarly journals Measurement of hindered diffusion in complex geometries for high-speed single-molecule experiments

2020 ◽  
Author(s):  
Tobias F. Bartsch ◽  
Camila M. Villasante ◽  
Ahmed Touré ◽  
Daniel M. Firester ◽  
Felicitas E. Hengel ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Molecule ◽  
High Speed ◽  
Optical Trap ◽  
Glass Coverslip ◽  
Hydrodynamic Coupling ◽  
Hindered Diffusion ◽  
Protocadherin 15 ◽  
Human Hearing ◽  
Infinite Wall

AbstractIn a high-speed single-molecule experiment, a protein is tethered between two substrates that are manipulated to exert force on the system. To avoid nonspecific interactions between the protein and nearby substrates, the protein is usually attached to the substrates through long, flexible linkers. This approach precludes measurements of mechanical properties with high spatial and temporal resolution, for rapidly exerted forces are dissipated into the linkers. Because mammalian hearing operates at frequencies reaching tens to hundreds of kilohertz, the mechanical processes that occur during transduction are of very short duration. Single-molecule experiments on the relevant proteins therefore cannot involve long tethers. We previously characterized the mechanical properties of protocadherin 15 (PCDH15), a protein essential for human hearing, by tethering an individual monomer through very short linkers between a probe bead held in an optical trap and a pedestal bead immobilized on a glass coverslip. Because the two confining surfaces were separated by only the length of the tethered protein, hydrodynamic coupling between those surfaces complicated the interpretation of the data. To facilitate our experiments, we characterize here the anisotropic and position-dependent diffusion coefficient of a probe in the presence of an effectively infinite wall, the coverslip, and of the immobile pedestal.

scholarly journals Single molecule mechanics resolves the earliest events in force generation by cardiac myosin

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Michael S Woody ◽  
Donald A Winkelmann ◽  
Marco Capitanio ◽  
E Michael Ostap ◽  
Yale E Goldman
Keyword(s):  
Single Molecule ◽  
High Speed ◽  
Atp Hydrolysis ◽  
Optical Trap ◽  
Force Generation ◽  
Actin Binding ◽  
Cardiac Myosin ◽  
Phosphate Release ◽  
Hydrolysis Products ◽  
Key Steps

Key steps of cardiac mechanochemistry, including the force-generating working stroke and the release of phosphate (Pi), occur rapidly after myosin-actin attachment. An ultra-high-speed optical trap enabled direct observation of the timing and amplitude of the working stroke, which can occur within <200 μs of actin binding by β-cardiac myosin. The initial actomyosin state can sustain loads of at least 4.5 pN and proceeds directly to the stroke or detaches before releasing ATP hydrolysis products. The rates of these processes depend on the force. The time between binding and stroke is unaffected by 10 mM Pi which, along with other findings, indicates the stroke precedes phosphate release. After Pi release, Pi can rebind enabling reversal of the working stroke. Detecting these rapid events under physiological loads provides definitive indication of the dynamics by which actomyosin converts biochemical energy into mechanical work.

scholarly journals Single molecule mechanics resolves the earliest events in force generation by cardiac myosin

2019 ◽  
Author(s):  
Michael S. Woody ◽  
Donald A. Winkelmann ◽  
Marco Capitanio ◽  
E. Michael Ostap ◽  
Yale E. Goldman
Keyword(s):  
Single Molecule ◽  
High Speed ◽  
Atp Hydrolysis ◽  
Optical Trap ◽  
Force Generation ◽  
Actin Binding ◽  
Cardiac Myosin ◽  
Phosphate Release ◽  
Hydrolysis Products ◽  
Key Steps

AbstractKey steps of cardiac mechanochemistry, including the force-generating working stroke and the release of phosphate (Pi), occur rapidly after myosin-actin attachment. An ultra-high-speed optical trap enabled direct observation of the timing and amplitude of the working stroke, which can occur within <200 μs of actin binding by β-cardiac myosin. The initial actomyosin state can sustain loads of at least 4.5 pN and proceeds directly to the stroke or detaches before releasing ATP hydrolysis products. The rates of these processes depend on the force. The time between binding and stroke is unaffected by 10 mM Pi which, along with other findings, indicates the stroke precedes phosphate release. After Pi release, Pi can rebind enabling reversal of the working stroke. Detecting these rapid events under physiological loads provides definitive indication of the dynamics by which actomyosin converts biochemical energy into mechanical work.

Influence of ageing treatment on microstructure, mechanical properties and adhesive wear behaviour of 6063 aluminium alloy

2014 ◽  
Vol 66 (4) ◽  
pp. 520-524 ◽  
Author(s):  
Serkan Büyükdoğan ◽  
Süleyman Gündüz ◽  
Mustafa Türkmen
Keyword(s):  
Mechanical Properties ◽  
Wear Resistance ◽  
High Speed ◽  
Al Alloys ◽  
Wear Behaviour ◽  
Al Alloy ◽  
Strain Ageing ◽  
Content Type ◽  
Static Strain ◽  
Structural Applications

Purpose – The paper aims to provide new observations about static strain ageing in aluminium (Al) alloys which are widely used in structural applications. Design/methodology/approach – The present work aims to provide theoretical and practical information to industries or researchers who may be interested in the effect of static strain ageing on mechanical properties of Al alloys. The data are sorted into the following sections: introduction, materials and experimental procedure, results and discussion and conclusions. Findings – Tensile strength, proof strength (0.2 per cent) and percentage elongation measurement were used to investigate the effect of strain ageing on the mechanical properties. Wear tests were performed by sliding the pin specimens, which were prepared from as-received, solution heat-treated, deformed and undeformed specimens after ageing, on high-speed tool steel (64 HRC). It is concluded that the variations in ageing time improved the strength and wear resistance of the 6063 Al alloy; however, a plastically deformed solution-treated alloy has higher strength and wear resistance than undeformed specimens for different ageing times at 180°C. Practical implications – A very useful source of information for industries using or planning to produce Al alloys. Originality/value – This paper fulfils an identified resource need and offers practical help to the industries.

scholarly journals Fiber Formation and Structural Development of HBA/HNA Thermotropic Liquid Crystalline Polymer in High-Speed Melt Spinning

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1134
Author(s):  
Bo Seok Song ◽  
Jun Young Lee ◽  
Sun Hwa Jang ◽  
Wan-Gyu Hahm
Keyword(s):  
Mechanical Properties ◽  
Shear Rate ◽  
High Speed ◽  
Melt Spinning ◽  
Liquid Crystalline Polymer ◽  
Liquid Crystalline ◽  
Crystalline Polymer ◽  
Fiber Formation ◽  
Structural Development ◽  
Thermotropic Liquid Crystalline Polymer

High-speed melt spinning of thermotropic liquid crystalline polymer (TLCP) resin composed of 4-hydroxybenzoic acid (HBA) and 2-hydroxy-6-napthoic acid (HNA) monomers in a molar ratio of 73/27 was conducted to investigate the characteristic structure development of the fibers under industrial spinning conditions, and the obtained as-spun TLCP fibers were analyzed in detail. The tensile strength and modulus of the fibers increased with shear rate in nozzle hole, draft in spin-line and spinning temperature and exhibited the high values of approximately 1.1 and 63 GPa, respectively, comparable to those of industrial as-spun TLCP fibers, at a shear rate of 70,000 s−1 and a draft of 25. X-ray diffraction demonstrated that the mechanical properties of the fibers increased with the crystalline orientation factor (fc) and the fractions of highly oriented crystalline and non-crystalline anisotropic phases. The results of structure analysis indicated that a characteristic skin–core structure developed at high drafts (i.e., spinning velocity) and low spinning temperatures, which contributed to weakening the mechanical properties of the TLCP fibers. It is supposed that this heterogeneous structure in the cross-section of the fibers was induced by differences in the cooling rates of the skin and core of the fiber in the spin-line.

scholarly journals Enhanced electrical conductivity and mechanical properties in thermally stable fine-grained copper wire

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Qingzhong Mao ◽  
Yusheng Zhang ◽  
Yazhou Guo ◽  
Yonghao Zhao
Keyword(s):  
Mechanical Properties ◽  
Thermal Stability ◽  
Electrical Conductivity ◽  
Yield Strength ◽  
High Speed ◽  
Copper Wire ◽  
Rapid Development ◽  
Dislocation Slip ◽  
Ultrafine Grains ◽  
The Wire

AbstractThe rapid development of high-speed rail requires copper contact wire that simultaneously possesses excellent electrical conductivity, thermal stability and mechanical properties. Unfortunately, these are generally mutually exclusive properties. Here, we demonstrate directional optimization of microstructure and overcome the strength-conductivity tradeoff in copper wire. We use rotary swaging to prepare copper wire with a fiber texture and long ultrafine grains aligned along the wire axis. The wire exhibits a high electrical conductivity of 97% of the international annealed copper standard (IACS), a yield strength of over 450 MPa, high impact and wear resistances, and thermal stability of up to 573 K for 1 h. Subsequent annealing enhances the conductivity to 103 % of IACS while maintaining a yield strength above 380 MPa. The long grains provide a channel for free electrons, while the low-angle grain boundaries between ultrafine grains block dislocation slip and crack propagation, and lower the ability for boundary migration.

scholarly journals Texture and mechanical properties of Cu alloy by cryogenic high-speed rolling

2021 ◽  
Vol 1121 (1) ◽  
pp. 012009
Author(s):  
S Lee ◽  
R Muchime ◽  
R Matsumoto ◽  
H Utsunomiya
Keyword(s):  
Mechanical Properties ◽  
High Speed ◽  
Cu Alloy

scholarly journals Prey Capturing Dynamics and Nanomechanically Graded Cutting Apparatus of Dragonfly Nymph

Materials ◽  
2021 ◽  
Vol 14 (3) ◽  
pp. 559
Author(s):  
Lakshminath Kundanati ◽  
Prashant Das ◽  
Nicola M. Pugno
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
High Speed ◽  
Prey Capture ◽  
High Speed Photography ◽  
Sensory Organs ◽  
Dragonfly Nymph ◽  
Drag Forces ◽  
Predatory Insects ◽  
Dragonfly Nymphs

Aquatic predatory insects, like the nymphs of a dragonfly, use rapid movements to catch their prey and it presents challenges in terms of movements due to drag forces. Dragonfly nymphs are known to be voracious predators with structures and movements that are yet to be fully understood. Thus, we examine two main mouthparts of the dragonfly nymph (Libellulidae: Insecta: Odonata) that are used in prey capturing and cutting the prey. To observe and analyze the preying mechanism under water, we used high-speed photography and, electron microscopy. The morphological details suggest that the prey-capturing labium is a complex grasping mechanism with additional sensory organs that serve some functionality. The time taken for the protraction and retraction of labium during prey capture was estimated to be 187 ± 54 ms, suggesting that these nymphs have a rapid prey mechanism. The Young’s modulus and hardness of the mandibles were estimated to be 9.1 ± 1.9 GPa and 0.85 ± 0.13 GPa, respectively. Such mechanical properties of the mandibles make them hard tools that can cut into the exoskeleton of the prey and also resistant to wear. Thus, studying such mechanisms with their sensory capabilities provides a unique opportunity to design and develop bioinspired underwater deployable mechanisms.

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