scholarly journals Livestock-associated MRSA survival on house flies (Musca domestica) and stable flies (Stomoxys calcitrans) after removal from a Danish pig farm

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jonno Jorn Stelder ◽  
Lene Jung Kjær ◽  
Lars Bogø Jensen ◽  
Anette Ella Boklund ◽  
Matt Denwood ◽  
...  
Keyword(s):  
House Fly ◽  
Event Analysis ◽  
Time To Event ◽  
House Flies ◽  
Stable Flies ◽  
Kaplan Meier ◽  
Distance Distributions ◽  
Close Proximity ◽  
Pig Farm ◽  
Over Time

AbstractWe caught stable- and house flies on a Danish LA-MRSA positive pig farm. Stable- and house flies were housed together and culled over time to test for the presence of live LA-MRSA bacteria at 24 h intervals to establish the length of time for which LA-MRSA can persist on flies. On average, 7% of stable flies and 27% of house flies tested positive for LA-MRSA immediately upon removal from the farm. LA-MRSA prevalence decreased over time and estimates based on a Kaplan–Meier time-to-event analysis indicated that the probability of a stable- or house fly testing positive for LA-MRSA was 5.4% and 7.8% after 24 h, 3.5% and 4.3% after 48 h, 3.1% and 2.2% after 72 h and 0.4% and 0% after 96 h of removal from the pig farm, respectively. Simultaneously, we found that caged cultivated house flies became carriers of LA-MRSA, without direct contact with pigs, in the same proportions as wild flies inside the farm. We provide distance distributions of Danish pig farms and residential addresses as well as the calculated maximum dispersal potentials of stable- and house flies, which suggest that there is a potential for stable- and house flies dispersing live LA-MRSA bacteria into the surrounding environment of a pig farm. This potential should therefore be considered when modelling the spread between farms or the risk posed to humans living in close proximity to LA-MRSA pig farm sources.

scholarly journals Review of Outcomes after Diagnosis of Malignancy in Kidney Transplant Patients: UNOS Database

2021 ◽  
Vol 2 (3) ◽  
pp. 253-263
Author(s):  
Het Patel ◽  
Nikhil Agrawal ◽  
Voravech Nissaisorakarn ◽  
Ridhi Gupta ◽  
Francesca Cardarelli
Keyword(s):  
Kidney Transplant ◽  
Graft Failure ◽  
Event Analysis ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Time To Event ◽  
Transplant Patients ◽  
Kaplan Meier ◽  
Lung Malignancy ◽  
Kidney Transplant Patients

Malignancy is the third major cause of death among transplant recipients. Patient and kidney transplant outcomes after the diagnosis of malignancy are not well described. We reviewed incidences and outcomes of colorectal, lung, PTLD, and renal malignancy after transplant among patients who received a transplant from January 2000 to December 2018 using the UNOS/OPTN database. Incidence of each malignancy was measured at 5 years and 10 years of transplant. The Kaplan–Meier curve was used for time-to-event analysis (graft and patient outcomes). Additionally, we sought to identify the causes of graft failure among these recipients. We found that 12,764 (5.5%) patients suffered malignancy, excluding squamous and basal cell skin carcinoma after transplant. During the first 5 years of transplant, incidence of colorectal, lung, PTLD, and renal malignancies was 2.99, 9.21, 15.61, and 8.55 per 10,000 person-years, respectively. Rates of graft failure were 10.3%, 7.6%, 19.9%, and 18.8%, respectively, among these patients at 5 years. Mortality rate was highest among patients who suffered lung malignancy (84%), followed by colorectal (61.5%), PTLD (49.1%), and renal (35.5%) at 5 years after diagnosis of malignancy. In conclusion, kidney transplant recipients diagnosed with lung malignancy have the lowest graft survival, compared to PTLD, colorectal, and renal malignancy. PTLD has the highest incidence rate in the first 5 years of transplant.

Survival analysis

2020 ◽  
pp. 181-218
Author(s):  
Bendix Carstensen
Keyword(s):  
Survival Analysis ◽  
Competing Risks ◽  
Cox Model ◽  
Survival Function ◽  
Event Analysis ◽  
Time To Event ◽  
Poisson Models ◽  
Kaplan Meier ◽  
Analysis Survival ◽  
Event Rates

This chapter describes survival analysis. Survival analysis concerns data where the outcome is a length of time, namely the time from inclusion in the study (such as diagnosis of some disease) till death or some other event — hence the term 'time to event analysis', which is also used. There are two primary targets normally addressed in survival analysis: survival probabilities and event rates. The chapter then looks at the life table estimator of survival function and the Kaplan–Meier estimator of survival. It also considers the Cox model and its relationship with Poisson models, as well as the Fine–Gray approach to competing risks.

SURVEY AND RELEASE OF PARASITOIDS (HYMENOPTERA) ATTACKING HOUSE AND STABLE FLIES (DIPTERA: MUSCIDAE) IN DAIRY OPERATIONS

1999 ◽  
Vol 131 (6) ◽  
pp. 743-756 ◽  
Author(s):  
Tanja McKay ◽  
Terry D. Galloway
Keyword(s):  
House Fly ◽  
Stomoxys Calcitrans ◽  
Stable Fly ◽  
Nasonia Vitripennis ◽  
Pupal Parasitoid ◽  
House Flies ◽  
Stable Flies ◽  
Naturally Occurring ◽  
The Status ◽  
No Releases

AbstractIn 1995, Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae), a commercially available pupal parasitoid of the house fly, Musca domestica L., and stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), was purchased to examine the status of wasps being sold to Manitoba producers. Percentage of pupae parasitized, numbers of parasitoids per pupa, total parasitoids, and parasitoid sex ratio were determined for each shipment of parasitoids received. To determine the extent to which these wasps could successfully parasitize house flies and stable flies, parasitoids were released weekly in four Manitoba dairy barns and levels of parasitism estimated. In 10 622 freeze-killed sentinel house fly pupae, 2.2% were parasitized throughout the season by N. vitripennis, and 5.8% were parasitized by eight other species of parasitoids. Of 11 897 naturally occurring house fly and stable fly pupae, 0.6% were parasitized by N. vitripennis, and 3.4% by eight other species of parasitoids. In four barns where there were no releases of N. vitripennis, 1.1% of 11 779 sentinel pupae were parasitized by four species of parasitoids and 3.8% of 8384 naturally occurring house fly and stable fly pupae were parasitized by nine species. The release of an estimated 3 648 093 N. vitripennis did not result in substantial parasitism in either sentinel pupae or naturally occurring pupae. In 1996, live sentinel house fly pupae (n = 50 842) and house fly and stable fly pupae occurring naturally (n = 4691) were collected in two of the nonrelease barns from the 1995 study to examine the activity of endemic parasitoids. Of the sentinel and naturally occurring pupae sampled, 4.0% and 9.4% were parasitized, respectively. Phygadeuon fumator Gravenhörst (Hymenoptera: Ichneumonidae) was the most abundant parasitoid, accounting for 97.4% and 79.9% of parasitoids collected from sentinel pupae and naturally occurring pupae, respectively. Other parasitoids included Urolepis rufipes (Ashmead), Muscidifurax raptor Girault and Sanders, Muscidifurax zaraptor Kogan and Legner, Spalangia subpunctata Först, Spalangia cameroni Perkins, Spalangia nigra Latreille, and a species of Trichomalopsis Crawford (Hymenoptera: Pteromalidae).

scholarly journals Proposals on Kaplan–Meier plots in medical research and a survey of stakeholder views: KMunicate

BMJ Open ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. e030215 ◽  
Author(s):  
Tim P Morris ◽  
Christopher I Jarvis ◽  
William Cragg ◽  
Patrick P J Phillips ◽  
Babak Choodari-Oskooei ◽  
...  
Keyword(s):  
Outcome Measures ◽  
Event Data ◽  
Time To Event ◽  
Web Based ◽  
Time To Event Data ◽  
Kaplan Meier ◽  
Starting Point ◽  
Wide Range ◽  
International Participation ◽  
Over Time

ObjectivesTo examine reactions to the proposed improvements to standard Kaplan–Meier plots, the standard way to present time-to-event data, and to understand which (if any) facilitated better depiction of (1) the state of patients over time, and (2) uncertainty over time in the estimates of survival.DesignA survey of stakeholders’ opinions on the proposals.SettingA web-based survey, open to international participation, for those with an interest in visualisation of time-to-event data.Participants1174 people participated in the survey over a 6-week period. Participation was global (although primarily Europe and North America) and represented a wide range of researchers (primarily statisticians and clinicians).Main outcome measuresTwo outcome measures were of principal importance: (1) participants’ opinions of each proposal compared with a ‘standard’ Kaplan–Meier plot; and (2) participants’ overall ranking of the proposals (including the standard).ResultsMost proposals were more popular than the standard Kaplan–Meier plot. The most popular proposals in the two categories, respectively, were an extended table beneath the plot depicting the numbers at risk, censored and having experienced an event at periodic timepoints, and CIs around each Kaplan–Meier curve.ConclusionsThis study produced a high response number, reflecting the importance of graphics for time-to-event data. Those producing and publishing Kaplan–Meier plots—both authors and journals—should, as a starting point, consider using the combination of the two favoured proposals.

Fractures and health care resource use in cancer patients with chemotherapy-associated peripheral neuropathy (CAPN).

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e13038-e13038
Author(s):  
Arijit Ganguli ◽  
Patrick J Reilly ◽  
Saurabh Ray
Keyword(s):  
Peripheral Neuropathy ◽  
Cancer Patients ◽  
Resource Use ◽  
Index Date ◽  
Event Analysis ◽  
Office Visits ◽  
Inclusion Criteria ◽  
Time To Event ◽  
Kaplan Meier ◽  
Increased Risk

e13038 Background: Chemotherapy has been associated with increased risk of fractures1. This study examines the real-world incidence of fractures and healthcare resource use (HRU) that may be associated with CAPN in cancer patients. Methods: A retrospective analysis utilized a national health insurer claims -database (2001-2009), to identify patients ≥18 yrs with a cancer ICD-9-code (140-239) and a chemotherapy drug code (J9xxx). The 1st chemotherapy date was the "index date." Patients with a record of peripheral neuropathy (PN) in the pre-index date were excluded. Patients with a PN post-index were matched with no-PN post-index (non-PN) based on gender, age and index date. Both groups were compared for number of fractures, HRU (hospital outpatient (OP), office, and emergency-room [ER] visits) and all-cause costs in their 365-days post-index period. Time to 1st fracture post-index was compared using Kaplan Meier time to event analysis. Results: Of 34,625 patient meeting the inclusion criteria, 1675 patients (4.3%) formed the PN group and were matched to non-PN group. At baseline, mean age was 54.9 yrs, 62.5% were females, and no difference in % of bone metastasis (p=0.12) between the groups. In PN group, 5.3% (n=87) had a fracture 365-days post-index compared to 3.5% (n=58) in non-PN group (p<0.05). Mean days to fracture from index date in PN group was shorter than the non-PN group (150.9 vs. 153.4, p<0.05). In PN group, annual mean number of OP visit (14.6 vs. 12.0, p<0.0001), ER visit (0.47 vs. 0.30, p<0.001), and office visits (30.4 vs. 23.3, p<0.0001), were higher compared to non-PN group. Annual healthcare cost of PN patients was 21% higher than non-PN patients ($64,578 vs. $53,221) and CAPN-related cost in PN group was estimated to be $5,580 annually. Conclusions: Patients with CAPN were associated with higher incidence of fractures, HRU and cost.

Time-to-Event Analyses: Return to Unrestricted Participation Following Sport-Related Concussion in a Cohort of High School Athletes

2020 ◽  
Author(s):  
Tracey Covassin ◽  
Abigail C. Bretzin ◽  
Erica Beidler ◽  
Jessica Wallace
Keyword(s):  
High School ◽  
High School Student ◽  
Student Athletes ◽  
Event Analysis ◽  
Time To Event ◽  
Injury Surveillance System ◽  
Kaplan Meier ◽  
Injury Reporting ◽  
Recovery Trajectory ◽  
High School Athletic

Abstract Context: Understanding time-loss resulting from sport-related concussion (SRC) within individual sports allows high school athletic trainers to provide accurate and clinically evidence-based information. Currently there is a lack of research regarding patterns of clinical recovery outcomes in high school student-athletes across sports. Objective: To describe the time to authorized unrestricted RTP following SRC in a large cohort of high school student-athletes in variety of sports using a time-to-event analysis. Design: Descriptive Epidemiology Study. Setting: Aggregate injury and player exposure data from the STATE-XXX High School Athletic Association (XHSAA) Head Injury Reporting System (HIRS). Patients or Other Participants: High school student-athletes. Main Outcome Measure(s): Dates for SRC injury events and authorized unrestricted RTP were entered into the HIRS for each case, and were used to calculate time to unrestricted RTP. Survival analysis determined time to authorized RTP for males and females in weekly increments across sports and academic years. Separate Kaplan-Meier analyses adjusted for SRC cases with a history of concussion also identified the proportions of student-athletes that obtained authorized medical clearance in weekly increments. Results: There was a total of 15,821 SRC cases, 10,375 (65.6%) male and 5,446 (34.4%) female, reported during the 2015–16 through 2018–19 academic years. The median time to authorized unrestricted RTP was 11 days for all cases. Approximately, 30% of concussed student-athletes were not cleared for unrestricted RTP by 14 days following their SRC diagnosis, with 13% taking longer than 21 days to unrestricted RTP after SRC. Conclusions: The results from this multi-site, State-based injury surveillance system indicate that it is not abnormal for high school student-athletes to take longer than 14 days to fully recovery from a SRC. This information may be useful for educating high school student-athletes and sport stakeholders, normalizing SRC recovery trajectory perceptions, and establishing realistic RTP timeline expectations.

scholarly journals Time-to-event analysis for sports injury research part 1: time-varying exposures

2018 ◽  
Vol 53 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Rasmus Oestergaard Nielsen ◽  
Michael Lejbach Bertelsen ◽  
Daniel Ramskov ◽  
Merete Møller ◽  
Adam Hulme ◽  
...  
Keyword(s):  
Sport Injury ◽  
Sports Injury ◽  
Training Load ◽  
Time Varying ◽  
Event Analysis ◽  
Time To Event ◽  
Effect Measure ◽  
Event Models ◽  
Analytical Approaches ◽  
Over Time

Background‘How much change in training load is too much before injury is sustained, among different athletes?’ is a key question in sports medicine and sports science. To address this question the investigator/practitioner must analyse exposure variables that change over time, such as change in training load. Very few studies have included time-varying exposures (eg, training load) and time-varying effect-measure modifiers (eg, previous injury, biomechanics, sleep/stress) when studying sports injury aetiology.AimTo discuss advanced statistical methods suitable for the complex analysis of time-varying exposures such as changes in training load and injury-related outcomes.ContentTime-varying exposures and time-varying effect-measure modifiers can be used in time-to-event models to investigate sport injury aetiology. We address four key-questions (i) Does time-to-event modelling allow change in training load to be included as a time-varying exposure for sport injury development? (ii) Why is time-to-event analysis superior to other analytical concepts when analysing training-load related data that changes status over time? (iii) How can researchers include change in training load in a time-to-event analysis? and, (iv) Are researchers able to include other time-varying variables into time-to-event analyses? We emphasise that cleaning datasets, setting up the data, performing analyses with time-varying variables and interpreting the results is time-consuming, and requires dedication. It may need you to ask for assistance from methodological peers as the analytical approaches presented this paper require specialist knowledge and well-honed statistical skills.ConclusionTo increase knowledge about the association between changes in training load and injury, we encourage sports injury researchers to collaborate with statisticians and/or methodological epidemiologists to carefully consider applying time-to-event models to prospective sports injury data. This will ensure appropriate interpretation of time-to-event data.

scholarly journals A pathogenic fungus uses volatiles to entice male flies into fatal matings with infected female cadavers

2021 ◽  
Author(s):  
Andreas Naundrup ◽  
Björn Bohman ◽  
Charles Kwadha ◽  
Annette Jensen ◽  
Paul G. Becher ◽  
...  
Keyword(s):  
Pathogenic Fungus ◽  
House Fly ◽  
Phenotypic Trait ◽  
Healthy Male ◽  
Entomophthora Muscae ◽  
Infected Female ◽  
House Flies ◽  
Close Proximity ◽  
Sexual Mimicry ◽  
Male House

To ensure dispersal, many parasites and pathogens behaviourally manipulate infected hosts. Other pathogens and certain insect-pollinated flowers use sexual mimicry and release deceptive mating signals. However, it is unusual for pathogens to rely on both behavioural host manipulation and sexual mimicry. Here, we show that the host-specific and behaviourally manipulating pathogenic fungus, Entomophthora muscae, generates a chemical blend of volatile sesquiterpenes and alters the level of natural host cuticular hydrocarbons in dead infected female house fly (Musca domestica) cadavers. Healthy male house flies respond to the fungal compounds and are enticed into mating with dead female cadavers. This is advantageous for the fungus as close proximity between host individuals leads to an increased probability of infection. The fungus-emitted volatiles thus represent the evolution of an extended phenotypic trait that exploit male flies' willingness to mate and benefit the fungus by altering the behavioural phenotype of uninfected healthy male host flies.

Review of Methods to Monitor House Fly (Musca domestica) Abundance and Activity

2020 ◽  
Vol 113 (6) ◽  
pp. 2571-2580 ◽  
Author(s):  
Alec C Gerry
Keyword(s):  
Management Program ◽  
House Fly ◽  
Pathogen Transmission ◽  
Urban Waste ◽  
House Flies ◽  
Large Numbers ◽  
Action Threshold ◽  
Routine Monitoring ◽  
Negative Impacts ◽  
Over Time

Abstract The house fly is a ubiquitous pest commonly associated with animal facilities and urban waste. When present in large numbers, house flies can negatively impact humans and animals through nuisance and the transmission of pathogens. Since the development of fly traps and sticky papers to capture flies in the late 1800s, these and other methods have been used as a means to monitor change in house fly density or fly activity over time. Methods include substrate sampling to record density of immature flies, visual observations of adult fly activity, instantaneous counts of landing or resting flies, accumulation of adult flies on/in traps, or accumulation of fly fecal and regurgitation spots deposited by flies onto white cards. These methods do not estimate true house fly density, but rather provide an index of house fly activity that is related to both fly density and the frequency of individual fly behavior (e.g., frequency of flight, landing events) and which is likely more predictive of negative impacts such as nuisance and pathogen transmission. Routine monitoring of house fly activity is a critical component of a house fly management program. Fly activity should be held to a level below a predetermined activity threshold ('action threshold') above which negative impacts are anticipated to occur. This article is a review of methods utilized for monitoring house fly (Diptera: Muscidae) activity.

Hymenopterous parasitoids of house fly and stable fly puparia in Prince Edward Island and New Brunswick, Canada

2007 ◽  
Vol 139 (5) ◽  
pp. 748-750 ◽  
Author(s):  
C. Noronha ◽  
G.A.P. Gibson ◽  
K.D. Floate
Keyword(s):  
New Brunswick ◽  
Prince Edward Island ◽  
Dairy Farms ◽  
House Fly ◽  
Stomoxys Calcitrans ◽  
Stable Fly ◽  
Additional Species ◽  
House Flies ◽  
Stable Flies ◽  
Percent Parasitism

AbstractPuparia of house flies, Musca domestica L., and stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae), were collected on dairy farms in Prince Edward Island (PEI) and New Brunswick (NB) and held for emergence of hymenopterous parasitoids. Percent parasitism for PEI in 2003 and 2004 was 6.7 (n = 10 060 puparia) and 1.0 (n = 36 992 puparia), respectively. Percent parasitism for NB was not determined in 2003, but was 9.1% (n = 3052 puparia) in 2004. A parasitoid provisionally identified as Phygadeuon ?fumator Gravenhörst (Ichneumonidae) predominated in both provinces. Additional species recovered included Aphaereta pallipes (Say) (Braconidae) and Muscidifurax raptor Girault and Saunders, Spalangia cameroni Perkins (PEI only), Spalangianigra Latreille (NB only), Spalangia subpunctata Förster (NB only), Trichomalopsis americana (Gahan) (PEI only), and Urolepis rufipes (Ashmead) (Pteromalidae). Dissection of host puparia from which neither flies nor wasps emerged yielded a relatively large number of additional parasitoids, particularly S. nigra.

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