scholarly journals Picochlorum celeri as a model system for robust outdoor algal growth in seawater

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anagha Krishnan ◽  
Maria Likhogrud ◽  
Melissa Cano ◽  
Scott Edmundson ◽  
Jenna B. Melanson ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
High Temperatures ◽  
Growth Rates ◽  
Saline Water ◽  
Algal Growth ◽  
Model System ◽  
Fast Growth ◽  
Sea Salts ◽  
Technology And Innovation ◽  
Raceway Ponds

AbstractWith fast growth rates, broad halotolerance and the ability to thrive at high temperatures, algae in the genus Picochlorum are emerging as promising biomass producers. Recently, we isolated a remarkably productive strain, Picochlorum celeri, that attains > 40 g m−2 day−1 productivities using simulated outdoor light. To test outdoor productivities, Picochlorum celeri was cultivated in 820 L raceway ponds at the Arizona Center for Algae Technology and Innovation. Picochlorum celeri demonstrated the highest outdoor biomass productivities reported to date at this testbed averaging ~ 31 g m−2 day−1 over four months with a monthly (August) high of ~ 36 g m−2 day−1. Several single day productivities were > 40 g m−2 day−1. Importantly for sustainability, Picochlorum celeri achieved these productivities in saline water ranging from seawater to 50 parts per thousand sea salts, without any biocides or pond crashes, for over 143 days. Lastly, we report robust genetic engineering tools for future strain improvements.

Comparison of Aminocyclopyrachlor to Common Herbicides for Kudzu (Pueraria montana) Management

2011 ◽  
Vol 4 (4) ◽  
pp. 419-426 ◽  
Author(s):  
Patrick J. Minogue ◽  
Stephen F. Enloe ◽  
Anna Osiecka ◽  
Dwight K. Lauer
Keyword(s):  
Growth Rates ◽  
Groundwater Pollution ◽  
Fast Growth ◽  
Control Measures ◽  
Volume Index ◽  
Metsulfuron Methyl ◽  
Herbicide Treatment ◽  
Herbicide Treatments ◽  
Repeated Applications ◽  
Pueraria Montana

AbstractKudzu is an invasive perennial climbing vine characterized by fast growth rates and tolerance to control measures. Repeated applications with high rates of 2,4-D plus picloram provide effective kudzu control, but picloram use is not permitted in certain states due to groundwater pollution concerns. Studies were conducted in Alabama and Florida to compare kudzu control with aminocyclopyrachlor, a new herbicide, to control provided by aminopyralid, clopyralid, metsulfuron methyl, and picloram plus 2,4-D, which are common treatments for kudzu management. Two annual applications of the same herbicide treatment were evaluated for effects on kudzu cover, kudzu volume index, and cover of other vegetation. Aminocyclopyrachlor at 140 to 280 g ae ha−1 (2 to 4 oz ae ac−1) was as effective as the standard 4.48 kg ae ha−1 (4 lb ae ac−1) 2,4-D amine plus 1.2 kg ae ha−1 picloram for kudzu control. There were no differences in kudzu control among the three rates of aminocyclopyrachlor tested. Colonization by graminoids, forbs, and Rubus spp. at 2 yr was greatest for herbicides providing the best kudzu control: aminocyclopyrachlor, and 2,4-D plus picloram. Herbicide treatments were more effective in controlling kudzu at the Alabama location, but repeated annual applications for 2 yr did not completely eliminate kudzu with any treatment at either site.

scholarly journals The Expanding Molecular Genetics Tool Kit in Chlamydia

2018 ◽  
Vol 200 (24) ◽  
Author(s):  
Raphael H. Valdivia ◽  
Robert J. Bastidas
Keyword(s):  
Genetic Engineering ◽  
Molecular Genetics ◽  
Genetic Manipulation ◽  
Molecular Genetic ◽  
Model System ◽  
New Method ◽  
Content Type ◽  
Host Interactions ◽  
Additional Tool ◽  
Genomic Regions

ABSTRACT Chlamydia has emerged as an important model system for the study of host pathogen interactions, in part due to a resurgence in the development of tools for its molecular genetic manipulation. An additional tool, published by Keb et al. (G. Keb, R. Hayman, and K. A. Fields, J. Bacteriol. 200:e00479-18, 2018, https://doi.org/10.1128/JB.00479-18), now allows for custom genetic engineering of genomic regions that were traditionally recalcitrant to genetic manipulation, such as genes within operons. This new method will be an essential instrument for the elucidation of Chlamydia-host interactions.

Dormancy, germination and seedling growth of two Kalaharian perennials of the genus Harpagophytum (Pedaliaceae)

1988 ◽  
Vol 4 (2) ◽  
pp. 185-198 ◽  
Author(s):  
W. H. O. Ernst ◽  
T. Tietema ◽  
E. M. Veenendaal ◽  
R. Masene
Keyword(s):  
Gibberellic Acid ◽  
High Temperatures ◽  
Seedling Growth ◽  
Seed Coat ◽  
Growth Rates ◽  
Natural Conditions ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Respiration Rates ◽  
Drought Avoidance

ABSTRACTDormancy and germination ecology of two Harpagophytum species (Pedaliaceae) from an open Acacia savanna in Botswana were investigated. The maintenance of dormancy is governed partly by the seed coat but mainly by the endosperm and the embryo itself, as demonstrated by removal of the endosperm. Dissemination of the seed from the fruit can be delayed for several years without affecting the viability of the embryo, due to very low respiration rates.Germination can be enhanced slightly by high temperatures under natural conditions, and by gibberellic acid or removal of the endosperm under laboratory conditions. Relative growth rates for both Harpagophytum species are lower than for subtropical grasses and legumes, due to a strong investment in root and tuber biomass. Germination and seedling growth is discussed in relation to the drought avoidance syndrome and the animal disperser syndrome.

scholarly journals Enhancement of the tolerance ofArabidopsisto high temperatures by genetic engineering of the synthesis of glycinebetaine

1998 ◽  
Vol 16 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Alia ◽  
Hidenori Hayashi ◽  
Atsushi Sakamoto ◽  
Norio Murata
Keyword(s):  
Genetic Engineering ◽  
High Temperatures

scholarly journals Cytoplasmic and Periplasmic Proteomic Signatures of Exponentially Growing Cells of the Psychrophilic BacteriumPseudoalteromonas haloplanktisTAC125

2010 ◽  
Vol 77 (4) ◽  
pp. 1276-1283 ◽  
Author(s):  
Boris Wilmes ◽  
Holger Kock ◽  
Susanne Glagla ◽  
Dirk Albrecht ◽  
Birgit Voigt ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Growth Rates ◽  
Stress Proteins ◽  
High Capacity ◽  
Tca Cycle ◽  
Fast Growth ◽  
Natural Environments ◽  
Permissive Temperature ◽  
Moderate Growth

ABSTRACTThe psychrophilic model bacteriumPseudoalteromonas haloplanktisis characterized by remarkably fast growth rates under low-temperature conditions in a range from 5°C to 20°C. In this study the proteome of cellular compartments, the cytoplasm and periplasm, ofP. haloplanktisstrain TAC125 was analyzed under exponential growth conditions at a permissive temperature of 16°C. By means of two-dimensional protein gel electrophoresis and mass spectrometry, a first inventory of the most abundant cytoplasmic and periplasmic proteins expressed in a peptone-supplemented minimal medium was established. By this approach major enzymes of the amino acid catabolism of this marine bacterium could be functionally deduced. The cytoplasmic proteome showed a predominance of amino acid degradation pathways and tricarboxylic acid (TCA) cycle enzymes but also the protein synthesis machinery. Furthermore, high levels of cold acclimation and oxidative stress proteins could be detected at this moderate growth temperature. The periplasmic proteome was characterized by a significant abundance of transporters, especially of highly expressed putative TonB-dependent receptors. This high capacity for protein synthesis, efficient amino acid utilization, and substrate transport may contribute to the fast growth rates of the copiotrophic bacteriumP. haloplanktisin its natural environments.

Directional solidification and microstructural studies of the peritectic Y2BaCuO5 phase

2001 ◽  
Vol 16 (4) ◽  
pp. 1123-1134 ◽  
Author(s):  
E. Sudhakar Reddy ◽  
J. G. Noudem ◽  
M. Tarka ◽  
G. J. Schmitz
Keyword(s):  
Liquid Phase ◽  
Directional Solidification ◽  
High Temperatures ◽  
Growth Rates ◽  
Solidification Rate ◽  
Microstructure Formation ◽  
Solidification Morphology ◽  
Microstructural Studies

Directional solidification using a Bridgman furnace was performed to produce textured Y2BaCuO5 (211) rods of both stoichiometric and off-stoichiometric compositions and to investigate microstructure formation at various solidification rates. The solidification morphology of the samples changed from planar to cellular and eventually to equiaxed blocky grains with increasing solidification rate. The microstructure of the stoichiometric 211 sample revealed elongated, aligned YBa2Cu3Oy (123) phase residual in the 211 matrix. The 211 samples rich in Y2O3 phase showed no trace of residual 123 but did show trapped Y2O3 particles. The morphology of the Y2O3 particles varied from spherical to a rodlike morphology as well along the length of a specific sample as also with decreasing growth rates in different samples. The Y2O3 particles in samples exposed for longer time to the liquid phase at high temperatures exhibited coarsening and unidirectional coalescence into a rodlike morphology and retained their morphology even in the 211 matrix after solidification.

ℵ0-categorical structures with arbitrarily fast growth of algebraic closure

2002 ◽  
Vol 67 (3) ◽  
pp. 897-909
Author(s):  
David M. Evans ◽  
M. E. Pantano
Keyword(s):  
Growth Rate ◽  
Automorphism Group ◽  
Finite Subset ◽  
Growth Rates ◽  
Algebraic Closure ◽  
Fast Growth ◽  
Permutation Groups ◽  
Natural Numbers ◽  
Categorical Structure ◽  
Image Position

Various results have been proved about growth rates of certain sequences of integers associated with infinite permutation groups. Most of these concern the number of orbits of the automorphism group of an ℵ0-categorical structure on the set of unordered n-subsets or on the set of n-tuples of elements of . (Recall that by the Ryll-Nardzewski Theorem, if is countable and ℵ0-categorical, the number of the orbits of its automorphism group Aut() on the set of n-tuples from is finite and equals the number of complete n-types consistent with the theory of .) The book [Ca90] is a convenient reference for these results. One of the oldest (in the realms of ‘folklore’) is that for any sequence (Kn)n∈ℕ of natural numbers there is a countable ℵ0-categorical structure such that the number of orbits of Aut() on the set of n-tuples from is greater than kn for all n.These investigations suggested the study of the growth rate of another sequence. Let be an ℵ0-categorical structure and X be a finite subset of . Let acl(X) be the algebraic closure of X, that is, the union of the finite X-definable subsets of . Equivalently, this is the union of the finite orbits on of Aut()(X), the pointwise stabiliser of X in Aut(). Define

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