scholarly journals X-ray Irradiated Vaccine Confers protection against Pneumonia caused by Pseudomonas Aeruginosa

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yanyan Li ◽  
Zhenling Wang ◽  
Xiaoxiao Liu ◽  
Jianying Tang ◽  
Bin Peng ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
X Ray

scholarly journals Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

2021 ◽  
Author(s):  
Davood Gheidari ◽  
Morteza Mehrdad ◽  
Saloomeh Maleki ◽  
Samanesadat Hosseini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Limited Area ◽  
X Ray Diffraction ◽  
X Ray ◽  
Inhibition Concentration ◽  
Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

scholarly journals Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa

2013 ◽  
Vol 135 (28) ◽  
pp. 10318-10321 ◽  
Author(s):  
Siseth Martínez-Caballero ◽  
Mijoon Lee ◽  
Cecilia Artola-Recolons ◽  
César Carrasco-López ◽  
Dusan Hesek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Reaction Products ◽  
X Ray ◽  
Virulence Determinant

Preliminary X-ray crystallographic study of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa

1989 ◽  
Vol 205 (3) ◽  
pp. 617-618 ◽  
Author(s):  
John J. Stezowski ◽  
Helmut Görisch ◽  
Zbigniew Dauter ◽  
Michael Rupp ◽  
Andrea Hoh ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
X Ray ◽  
Crystallographic Study ◽  
Ethanol Dehydrogenase

Crystallization and preliminary X-ray crystallographic analysis of the Pseudomonas aeruginosa cyclohexadienyl dehydratase

1999 ◽  
Vol 55 (2) ◽  
pp. 539-541
Author(s):  
Palangpon Kongsaeree ◽  
Jun Liang ◽  
Roy A. Jensen ◽  
Jon Clardy
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crystal Form ◽  
High Energy ◽  
Crystallographic Analysis ◽  
Unit Cell ◽  
Space Group ◽  
X Ray ◽  
Cell Dimensions ◽  
Unit Cell Dimensions

The title protein has been crystallized in a new crystal form. The crystals belong to the cubic space group P4132 (or P4332) with unit-cell dimensions a = b = c = 126.1 Å at 100 K and typically diffract beyond 1.6 Å at the Cornell High Energy Synchotron Source (CHESS) A1 beamline.

scholarly journals Spatial Structure and Antimicrobial Activity of Cyclopropane Derivative of Limonene

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300
Author(s):  
Daniyar Sadyrbekov ◽  
Timur Saliev ◽  
Yuri Gatilov ◽  
Ivan Kulakov ◽  
Roza Seidakhmetova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Spatial Structure ◽  
Bacterial Strains ◽  
Gram Negative ◽  
X Ray ◽  
Cyclopropane Derivative

A cyclopropane derivative of limonene, (1 S, 4 S, 6 R)-7,7-dichloro-4-[(1 S)-2,2-dichloro-1-methylcyclopropyl]-1-methylbicyclo [4.1.0] heptane (compound 2), was synthesized and its structure was determined by NMR and X-ray crystallographic methods. In addition, an antimicrobial activity of the compound against Gram-positive ( Staphylococcus aureus, Bacillus subtilis) and Gram-negative ( Escherichia coli, Pseudomonas aeruginosa) bacterial strains was also scrutinized.

Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

1991 ◽  
Vol 222 (4) ◽  
pp. 869-871 ◽  
Author(s):  
Stuart A. Wilson ◽  
Naomi E. Chayen ◽  
Andrew M. Hemmings ◽  
Robert E. Drew ◽  
Laurence H. Pearl
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Negative Regulator ◽  
X Ray

scholarly journals Characterization of βαβββ Resistance Proteins from Pseudomonas aeruginosa

2014 ◽  
Vol 70 (a1) ◽  
pp. C717-C717
Author(s):  
Allegra Vit ◽  
Monika Popp ◽  
Eyad Kalawy-Fansa ◽  
Shen Yu ◽  
Wulf Blankenfeldt
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Physiological Function ◽  
Functional Characterization ◽  
Conserved Residues ◽  
X Ray ◽  
Resistance Proteins ◽  
X Ray Crystallography ◽  
Phenazine Derivative ◽  
Function Assignment

Pseudomonas aeruginosa is a multiresistant pathogen that can cause infection in immuno-compromized patients, for example in people suffering from cystic fibrosis. [1] It has complex patho-physiology and produces a large number of exoproducts, among which the phenazines are especially prominent. In P. aeruginosa, the blue phenazine derivative pyocyanin plays a crucial role in infection of the host. [2] This phenazine can generate reactive oxygen species and is thought to act as respiratory pigment and as a virulence factor at the same time. P. aeruginosa has to protect itself from its own phenazines because of the antibiotic action of these substances. Inspired by the fact that the phenazine biosynthesis operon of several bacteria contains a phenazine resistance factor of the βαβββ module protein family, we have searched the genome of P. aeruginosa for proteins of this fold. [3] In P. aeruginosa we could identify 22 of these genes, most without previous functional characterization. A structure-based sequence alignment made it possible to assign these proteins to two classes with two subgroups each, based on the conserved residues in the active site. Using X-ray crystallography and biophysical methods, we further demonstrate that several of these proteins indeed bind phenazines and possibly other antibiotics that contain aromatic moieties. Currently, we are working on the structural characterization and physiological function assignment of all of these βαβββ-module-containing proteins. Ultimately, these data may lead to novel anti-infective strategies.

scholarly journals Biochemical and Structural Characterization of the Subclass B1 Metallo-β-Lactamase VIM-4

2010 ◽  
Vol 55 (3) ◽  
pp. 1248-1255 ◽  
Author(s):  
Patricia Lassaux ◽  
Daouda A. K. Traoré ◽  
Elodie Loisel ◽  
Adrien Favier ◽  
Jean-Denis Docquier ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Escherichia Coli ◽  
Thermal Stability ◽  
Pseudomonas Aeruginosa ◽  
Structural Characterization ◽  
Catalytic Efficiency ◽  
X Ray ◽  
Stabilizing Effect ◽  
Loop 2

ABSTRACTThe metallo-β-lactamase VIM-4, mainly found inPseudomonas aeruginosaorAcinetobacter baumannii, was produced inEscherichia coliand characterized by biochemical and X-ray techniques. A detailed kinetic study performed in the presence of Zn2+at concentrations ranging from 0.4 to 100 μM showed that VIM-4 exhibits a kinetic profile similar to the profiles of VIM-2 and VIM-1. However, VIM-4 is more active than VIM-1 against benzylpenicillin, cephalothin, nitrocefin, and imipenem and is less active than VIM-2 against ampicillin and meropenem. The crystal structure of the dizinc form of VIM-4 was solved at 1.9 Å. The sole difference between VIM-4 and VIM-1 is found at residue 228, which is Ser in VIM-1 and Arg in VIM-4. This substitution has a major impact on the VIM-4 catalytic efficiency compared to that of VIM-1. In contrast, the differences between VIM-2 and VIM-4 seem to be due to a different position of the flapping loop and two substitutions in loop 2. Study of the thermal stability and the activity of the holo- and apo-VIM-4 enzymes revealed that Zn2+ions have a pronounced stabilizing effect on the enzyme and are necessary for preserving the structure.

scholarly journals The canal inclusion complex of allicin with carbamide: Preparation, characterization and microbiological investigation

2005 ◽  
Vol 11 (2) ◽  
pp. 69-73 ◽  
Author(s):  
Vesna Nikolic ◽  
Mihajlo Stankovic ◽  
Ljubisa Nikolic ◽  
Dragan Cvetkovic ◽  
Agnes Kapor ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Inclusion Complex ◽  
Microbiological Investigation ◽  
X Ray ◽  
X Ray Crystallography ◽  
Bacteria And Fungi ◽  
Growth Of Bacteria

The carbamide:allicin canal inclusion complex was prepared in the solid state. The structure of the complex obtained was characterized by x-ray crystallography, infrared spectroscopy and thermogravimetric analysis. The microbiological activities of the inclusion complex and allicin were investigated and compared with respect to fungi (Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404) and bacteria (Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027). It was found that the inclusion complex inhibited the growth of bacteria and fungi for a longer period than allicin in the free state.

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