scholarly journals Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Feng Zhang ◽  
Guozhong Zhu ◽  
Lei Du ◽  
Xiaoguang Shang ◽  
Chaoze Cheng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Genetic Regulation ◽  
Ion Homeostasis ◽  
Rna Seq ◽  
Salt Stress Tolerance ◽  
Crop Resistance ◽  
Cotton Species ◽  
Diploid Cotton

Abstract Cotton is an economically important crop throughout the world and is a pioneer crop in salt stress tolerance research. Investigation of the genetic regulation of salinity tolerance will provide information for salt stress-resistant breeding. Here, we employed next-generation RNA-Seq technology to elucidate the salt-tolerant mechanisms in cotton using the diploid cotton species Gossypium davidsonii which has superior stress tolerance. A total of 4744 and 5337 differentially expressed genes (DEGs) were found to be involved in salt stress tolerance in roots and leaves, respectively. Gene function annotation elucidated salt overly sensitive (SOS) and reactive oxygen species (ROS) signaling pathways. Furthermore, we found that photosynthesis pathways and metabolism play important roles in ion homeostasis and oxidation balance. Moreover, our studies revealed that alternative splicing also contributes to salt-stress responses at the posttranscriptional level, implying its functional role in response to salinity stress. This study not only provides a valuable resource for understanding the genetic control of salt stress in cotton, but also lays a substantial foundation for the genetic improvement of crop resistance to salt stress.

scholarly journals Melatonin: A Small Molecule but Important for Salt Stress Tolerance in Plants

2019 ◽  
Vol 20 (3) ◽  
pp. 709 ◽  
Author(s):  
Haoshuang Zhan ◽  
Xiaojun Nie ◽  
Ting Zhang ◽  
Shuang Li ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Ion Homeostasis ◽  
Free Radical Scavenger ◽  
Limiting Factors ◽  
Polyamine Metabolism ◽  
Antioxidant Systems ◽  
Radical Scavenger ◽  
Salt Stress Tolerance

Salt stress is one of the most serious limiting factors in worldwide agricultural production, resulting in huge annual yield loss. Since 1995, melatonin (N-acetyl-5-methoxytryptamine)—an ancient multi-functional molecule in eukaryotes and prokaryotes—has been extensively validated as a regulator of plant growth and development, as well as various stress responses, especially its crucial role in plant salt tolerance. Salt stress and exogenous melatonin lead to an increase in endogenous melatonin levels, partly via the phyto-melatonin receptor CAND2/PMTR1. Melatonin plays important roles, as a free radical scavenger and antioxidant, in the improvement of antioxidant systems under salt stress. These functions improve photosynthesis, ion homeostasis, and activate a series of downstream signals, such as hormones, nitric oxide (NO) and polyamine metabolism. Melatonin also regulates gene expression responses to salt stress. In this study, we review recent literature and summarize the regulatory roles and signaling networks involving melatonin in response to salt stress in plants. We also discuss genes and gene families involved in the melatonin-mediated salt stress tolerance.

scholarly journals Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis

2020 ◽  
Vol 21 (5) ◽  
pp. 1595 ◽  
Author(s):  
Hye-Yeon Seok ◽  
Linh Vu Nguyen ◽  
Doai Van Nguyen ◽  
Sun-Young Lee ◽  
Yong-Hwan Moon
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Rna Helicase ◽  
Rna Seq ◽  
Salt Stress Tolerance ◽  
Salt Stress Condition ◽  
Dead Box ◽  
Helicase Gene ◽  
Upregulated Genes

Previously, we reported that overexpression of AtRH17, an Arabidopsis DEAD-box RNA helicase gene, confers salt stress-tolerance via a pathway other than the well-known salt stress-responsive pathways. To decipher the salt stress-responsive pathway in AtRH17-overexpressing transgenic plants (OXs), we performed RNA-Sequencing and identified 397 differentially expressed genes between wild type (WT) and AtRH17 OXs. Among them, 286 genes were upregulated and 111 genes were downregulated in AtRH17 OXs relative to WT. Gene ontology annotation enrichment and KEGG pathway analysis showed that the 397 upregulated and downregulated genes are involved in various biological functions including secretion, signaling, detoxification, metabolic pathways, catabolic pathways, and biosynthesis of secondary metabolites as well as in stress responses. Genevestigator analysis of the upregulated genes showed that nine genes, namely, LEA4-5, GSTF6, DIN2/BGLU30, TSPO, GSTF7, LEA18, HAI1, ABR, and LTI30, were upregulated in Arabidopsis under salt, osmotic, and drought stress conditions. In particular, the expression levels of LEA4-5, TSPO, and ABR were higher in AtRH17 OXs than in WT under salt stress condition. Taken together, our results suggest that a high AtRH17 expression confers salt stress-tolerance through a novel salt stress-responsive pathway involving nine genes, other than the well-known ABA-dependent and ABA-independent pathways.

scholarly journals Manganese-induced salt stress tolerance in rice seedlings: regulation of ion homeostasis, antioxidant defense and glyoxalase systems

2016 ◽  
Vol 22 (3) ◽  
pp. 291-306 ◽  
Author(s):  
Anisur Rahman ◽  
Md. Shahadat Hossain ◽  
Jubayer-Al Mahmud ◽  
Kamrun Nahar ◽  
Mirza Hasanuzzaman ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Antioxidant Defense ◽  
Ion Homeostasis ◽  
Rice Seedlings ◽  
Salt Stress Tolerance

scholarly journals Functional Characterization of a Sugar Beet BvbHLH93 Transcription Factor in Salt Stress Tolerance

2021 ◽  
Vol 22 (7) ◽  
pp. 3669
Author(s):  
Yuguang Wang ◽  
Shuang Wang ◽  
Ye Tian ◽  
Qiuhong Wang ◽  
Sixue Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Sugar Beet ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Functional Characterization ◽  
Bhlh Transcription Factor ◽  
Salt Stress Tolerance ◽  
Antioxidant Genes

The basic/helix–loop–helix (bHLH) transcription factor (TF) plays an important role for plant growth, development, and stress responses. Previously, proteomics of NaCl treated sugar beet leaves revealed that a bHLH TF, BvbHLH93, was significantly increased under salt stress. The BvbHLH93 protein localized in the nucleus and exhibited activation activity. The expression of BvbHLH93 was significantly up-regulated in roots and leaves by salt stress, and the highest expression level in roots and leaves was 24 and 48 h after salt stress, respectively. Furthermore, constitutive expression of BvbHLH93 conferred enhanced salt tolerance in Arabidopsis, as indicated by longer roots and higher content of chlorophyll than wild type. Additionally, the ectopic expression lines accumulated less Na+ and MDA, but more K+ than the WT. Overexpression of the BvBHLH93 enhanced the activities of antioxidant enzymes by positively regulating the expression of antioxidant genes SOD and POD. Compared to WT, the overexpression plants also had low expression levels of RbohD and RbohF, which are involved in reactive oxygen species (ROS) production. These results suggest that BvbHLH93 plays a key role in enhancing salt stress tolerance by enhancing antioxidant enzymes and decreasing ROS generation.

scholarly journals Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Binghui Shan ◽  
Wei Wang ◽  
Jinfeng Cao ◽  
Siqi Xia ◽  
Ruihua Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Agronomic Traits ◽  
Salt Stress Tolerance ◽  
Developmental Defects ◽  
Functional Roles ◽  
Regulatory Module

REVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. However, the knowledge regarding the functional roles of RVEs is extremely limited in soybean. In this study, the soybean gene GmMYB133 was shown to be homologous to the RVE8 clade genes of Arabidopsis. GmMYB133 displayed a non-rhythmical but salt-inducible expression pattern. Like AtRVE8, overexpression of GmMYB133 in Arabidopsis led to developmental defects such as short hypocotyl and late flowering. Seven light-responsive or auxin-associated genes including AtPIF4 were transcriptionally depressed by GmMYB133, suggesting that GmMYB133 might negatively regulate plant growth. Noticeably, the overexpression of GmMYB133 in Arabidopsis promoted seed germination and plant growth under salt stress, and the contents of chlorophylls and malondialdehyde (MDA) were also enhanced and decreased, respectively. Consistently, the expressions of four positive regulators responsive to salt tolerance were remarkably elevated by GmMYB133 overexpression, indicating that GmMYB133 might confer salt stress tolerance. Further observation showed that GmMYB133 overexpression perturbed the clock rhythm of AtPRR5, and yeast one-hybrid assay indicated that GmMYB133 could bind to the AtPRR5 promoter. Moreover, the retrieved ChIP-Seq data showed that AtPRR5 could directly target five clients including AtPIF4. Thus, a regulatory module GmMYB133-PRR5-PIF4 was proposed to regulate plant growth and salt stress tolerance. These findings laid a foundation to further address the functional roles of GmMYB133 and its regulatory mechanisms in soybean.

scholarly journals GarWRKY5, a Member of the WRKY Transcription Factor Gene Family from a Diploid Cotton Species (Gossypium aridum L.), Is Involved in Salt Stress Response

2019 ◽  
Author(s):  
Qi Guo ◽  
Liang Zhao ◽  
Xinqi Fan ◽  
Peng Xu ◽  
Zhenzhen Xu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Stress Response ◽  
Jasmonic Acid ◽  
Virus Induced Gene Silencing ◽  
Wild Type ◽  
Salt Stress Tolerance ◽  
Salt Stress Response ◽  
Cotton Species ◽  
Diploid Cotton

Abstract Background Cotton is one of the most economically important crops in the world, and it is exposed to various abiotic stresses during its lifecycle, especially salt stress. However, the molecular mechanisms underlying cotton tolerance to salt stress are still not fully understood due to the complex nature of salt response. Therefore, identification of salt stress-tolerance-related functional genes will help us to understand key components involved in stress response and to provide valuable genes for salt stress tolerance improvement via genetic engineering in cotton. In a previous study, expression of a Group III WRKY gene family member from the diploid cotton species Gossypium aridum, GarWRKY5, was significantly induced in response to salt stress. Results In this present study, virus-induced gene silencing of GarWRKY5 in cotton showed enhanced salt sensitivity compared to wild-type plants under salt stress. Overexpression of GarWRKY5 in Arabidopsis positively regulated salt tolerance at the stages of seed germination and vegetative growth. Additionally, GarWRKY5-overexpressing plants exhibited higher activities of superoxide dismutase (SOD) and peroxidase (POD) under salt stress. The transcriptome sequencing analysis of transgenic Arabidopsis plants and wild-type plants revealed that there was enriched co-expression of genes involved in reactive oxygen species (ROS) scavenging (including glutamine S-transferases (GSTs) and SODs) and altered response to jasmonic acid and salicylic acid in the GarWRKY5-OE lines. Conclusion GarWRKY5 is involved in salt stress response by the jasmonic acid- or salicylic acid-mediated signaling pathway based on overexpression of GarWRKY5 in Arabidopsis and virus-induced gene silencing of GarWRKY5 in cotton.

scholarly journals Salt-Induced Damage is Alleviated by Short-Term Pre-Cold Treatment in Bermudagrass (Cynodon dactylon)

Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 347
Author(s):  
Jibiao Fan ◽  
Jilei Xu ◽  
Weihong Zhang ◽  
Maurice Amee ◽  
Dalin Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Cynodon Dactylon ◽  
Cold Treatment ◽  
Ion Homeostasis ◽  
Salt Stress Tolerance ◽  
Land Utilization ◽  
Short Term ◽  
Positive Role ◽  
Cell Membrane Stability

Excess salinity is a major environmental stress that limits growth and development of plants. Improving salt stress tolerance of plants is important in order to enhance land utilization and crop yield. Cold priming has been reported to trigger the protective processes in plants that increase their stress tolerance. Bermudagrass (Cynodon dactylon) is one of the most widely used turfgrass species around the world. However, the effect of cold priming on salt tolerance of bermudagrass is largely unknown. In the present study, wild bermudagrass was pre-treated with 4 °C for 6 h before 150 mM NaCl treatment for one week. The results showed that the cell membrane stability, ion homeostasis and photosynthesis process which are usually negatively affected by salt stress in bermudagrass were alleviated by short-term pre-cold treatment. Additionally, the gene expression profile also corresponded to the change of physiological indexes in bermudagrass. The results suggest that cold priming plays a positive role in improving salt stress tolerance of bermudagrass.

scholarly journals Overexpression of the DEAD-Box RNA Helicase Gene AtRH17 Confers Tolerance to Salt Stress in Arabidopsis

2018 ◽  
Vol 19 (12) ◽  
pp. 3777 ◽  
Author(s):  
Linh Nguyen ◽  
Hye-Yeon Seok ◽  
Dong-Hyuk Woo ◽  
Sun-Young Lee ◽  
Yong-Hwan Moon
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Rna Helicase ◽  
Activation Tagging ◽  
Salt Stress Tolerance ◽  
Salt Stress Response ◽  
Dead Box ◽  
Stress Responsive Genes

Plants adapt to abiotic stresses by complex mechanisms involving various stress-responsive genes. Here, we identified a DEAD-box RNA helicase (RH) gene, AtRH17, in Arabidopsis, involved in salt-stress responses using activation tagging, a useful technique for isolating novel stress-responsive genes. AT895, an activation tagging line, was more tolerant than wild type (WT) under NaCl treatment during germination and seedling development, and AtRH17 was activated in AT895. AtRH17 possesses nine well-conserved motifs of DEAD-box RHs, consisting of motifs Q, I, Ia, Ib, and II-VI. Although at least 12 orthologs of AtRH17 have been found in various plant species, no paralog occurs in Arabidopsis. AtRH17 protein is subcellularily localized in the nucleus. AtRH17-overexpressing transgenic plants (OXs) were more tolerant to high concentrations of NaCl and LiCl compared with WT, but no differences from WT were detected among seedlings exposed to mannitol and freezing treatments. Moreover, in the mature plant stage, AtRH17 OXs were also more tolerant to NaCl than WT, but not to drought, suggesting that AtRH17 is involved specifically in the salt-stress response. Notably, transcriptions of well-known abscisic acid (ABA)-dependent and ABA-independent stress-response genes were similar or lower in AtRH17 OXs than WT under salt-stress treatments. Taken together, our findings suggest that AtRH17, a nuclear DEAD-box RH protein, is involved in salt-stress tolerance, and that its overexpression confers salt-stress tolerance via a pathway other than the well-known ABA-dependent and ABA-independent pathways.

Sign in / Sign up

Export Citation Format

Share Document