scholarly journals GarWRKY5, a Member of the WRKY Transcription Factor Gene Family from a Diploid Cotton Species (Gossypium aridum L.), Is Involved in Salt Stress Response

2019 ◽  
Author(s):  
Qi Guo ◽  
Liang Zhao ◽  
Xinqi Fan ◽  
Peng Xu ◽  
Zhenzhen Xu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Stress Response ◽  
Jasmonic Acid ◽  
Virus Induced Gene Silencing ◽  
Wild Type ◽  
Salt Stress Tolerance ◽  
Salt Stress Response ◽  
Cotton Species ◽  
Diploid Cotton

Abstract Background Cotton is one of the most economically important crops in the world, and it is exposed to various abiotic stresses during its lifecycle, especially salt stress. However, the molecular mechanisms underlying cotton tolerance to salt stress are still not fully understood due to the complex nature of salt response. Therefore, identification of salt stress-tolerance-related functional genes will help us to understand key components involved in stress response and to provide valuable genes for salt stress tolerance improvement via genetic engineering in cotton. In a previous study, expression of a Group III WRKY gene family member from the diploid cotton species Gossypium aridum, GarWRKY5, was significantly induced in response to salt stress. Results In this present study, virus-induced gene silencing of GarWRKY5 in cotton showed enhanced salt sensitivity compared to wild-type plants under salt stress. Overexpression of GarWRKY5 in Arabidopsis positively regulated salt tolerance at the stages of seed germination and vegetative growth. Additionally, GarWRKY5-overexpressing plants exhibited higher activities of superoxide dismutase (SOD) and peroxidase (POD) under salt stress. The transcriptome sequencing analysis of transgenic Arabidopsis plants and wild-type plants revealed that there was enriched co-expression of genes involved in reactive oxygen species (ROS) scavenging (including glutamine S-transferases (GSTs) and SODs) and altered response to jasmonic acid and salicylic acid in the GarWRKY5-OE lines. Conclusion GarWRKY5 is involved in salt stress response by the jasmonic acid- or salicylic acid-mediated signaling pathway based on overexpression of GarWRKY5 in Arabidopsis and virus-induced gene silencing of GarWRKY5 in cotton.

scholarly journals Transcription Factor GarWRKY5 Is Involved in Salt Stress Response in Diploid Cotton Species (Gossypium aridum L.)

2019 ◽  
Vol 20 (21) ◽  
pp. 5244 ◽  
Author(s):  
Qi Guo ◽  
Liang Zhao ◽  
Xinqi Fan ◽  
Peng Xu ◽  
Zhenzhen Xu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Stress Response ◽  
Jasmonic Acid ◽  
Gene Silencing ◽  
Stress Tolerance ◽  
Virus Induced Gene Silencing ◽  
Wild Type ◽  
Salt Stress Tolerance ◽  
Salt Stress Response

Cotton is one of the most economically important crops in the world, and it is exposed to various abiotic stresses during its lifecycle, especially salt stress. However, the molecular mechanisms underlying cotton tolerance to salt stress are still not fully understood due to the complex nature of salt response. Therefore, identification of salt stress tolerance-related functional genes will help us understand key components involved in stress response and provide valuable genes for improving salt stress tolerance via genetic engineering in cotton. In the present study, virus-induced gene silencing of GhWRKY5 in cotton showed enhanced salt sensitivity compared to wild-type plants under salt stress. Overexpression of GarWRKY5 in Arabidopsis positively regulated salt tolerance at the stages of seed germination and vegetative growth. Additionally, GarWRKY5-overexpressing plants exhibited higher activities of superoxide dismutase (SOD) and peroxidase (POD) under salt stress. The transcriptome sequencing analysis of transgenic Arabidopsis plants and wild-type plants revealed that there was enriched coexpression of genes involved in reactive oxygen species (ROS) scavenging (including glutamine S-transferases (GSTs) and SODs) and altered response to jasmonic acid and salicylic acid in the GarWRKY5-OE lines. GarWRKY5 is involved in salt stress response by the jasmonic acid- or salicylic acid-mediated signaling pathway based on overexpression of GarWRKY5 in Arabidopsis and virus-induced gene silencing of GarWRKY5 in cotton.

scholarly journals Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Forests ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 423 ◽  
Author(s):  
Shupei Rao ◽  
Chao Du ◽  
Aijia Li ◽  
Xinli Xia ◽  
Weilun Yin ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Stress Response ◽  
Protein Kinase ◽  
Abiotic Stresses ◽  
Growth And Development ◽  
Populus Euphratica ◽  
Mitogen Activated Protein Kinase ◽  
Salt Stress Response

Populus euphratica Oliv. is a model tree for studying abiotic stress, especially salt stress response. Salt stress is one of the most extensive abiotic stresses, which has an adverse effect on plant growth and development. Salicylic acid (SA) is an important signaling molecule that plays an important role in modulating the plant responses to abiotic stresses. To answer whether the endogenous SA can be induced by salt stress, and whether SA effectively alleviates the negative effects of salt on poplar growth is the main purpose of the study. To elucidate the effects of SA and salt stress on the growth of P. euphratica, we examined the morphological and physiological changes of P. euphratica under 300 mM NaCl after treatment with different concentrations of SA. A pretreatment of P. euphratica with 0.4 mM SA for 3 days effectively improved the growth status of plants under subsequent salt stress. These results indicate that appropriate concentrations of exogenous SA can effectively counteract the negative effect of salt stress on growth and development. Subsequently, transcripts involved in salt stress response via SA signaling were captured by RNA sequencing. The results indicated that numerous specific genes encoding mitogen-activated protein kinase, calcium-dependent protein kinase, and antioxidant enzymes were upregulated. Potassium transporters and Na+/H+ antiporters, which maintain K+/Na+ balance, were also upregulated after SA pretreatment. The transcriptome changes show that the ion transport and antioxidant enzymes were the early enhanced systems in response of P. euphratica to salt via SA, expanding our knowledge about SA function in salt stress defense in P. euphratica. This provides a solid foundation for future study of functional genes controlling effective components in metabolic pathways of trees.

scholarly journals Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Feng Zhang ◽  
Guozhong Zhu ◽  
Lei Du ◽  
Xiaoguang Shang ◽  
Chaoze Cheng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Genetic Regulation ◽  
Ion Homeostasis ◽  
Rna Seq ◽  
Salt Stress Tolerance ◽  
Crop Resistance ◽  
Cotton Species ◽  
Diploid Cotton

Abstract Cotton is an economically important crop throughout the world and is a pioneer crop in salt stress tolerance research. Investigation of the genetic regulation of salinity tolerance will provide information for salt stress-resistant breeding. Here, we employed next-generation RNA-Seq technology to elucidate the salt-tolerant mechanisms in cotton using the diploid cotton species Gossypium davidsonii which has superior stress tolerance. A total of 4744 and 5337 differentially expressed genes (DEGs) were found to be involved in salt stress tolerance in roots and leaves, respectively. Gene function annotation elucidated salt overly sensitive (SOS) and reactive oxygen species (ROS) signaling pathways. Furthermore, we found that photosynthesis pathways and metabolism play important roles in ion homeostasis and oxidation balance. Moreover, our studies revealed that alternative splicing also contributes to salt-stress responses at the posttranscriptional level, implying its functional role in response to salinity stress. This study not only provides a valuable resource for understanding the genetic control of salt stress in cotton, but also lays a substantial foundation for the genetic improvement of crop resistance to salt stress.

scholarly journals Transcriptome profiling at osmotic and ionic phases of salt stress response in bread wheat uncovers trait-specific candidate genes

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Diana Duarte-Delgado ◽  
Said Dadshani ◽  
Heiko Schoof ◽  
Benedict C. Oyiga ◽  
Michael Schneider ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Stress Tolerance ◽  
Candidate Genes ◽  
Bread Wheat ◽  
Calcium Binding ◽  
Molecular Mechanisms ◽  
Salt Stress Tolerance ◽  
Salt Stress Response ◽  
Tolerant Genotype

Abstract Background Bread wheat is one of the most important crops for the human diet, but the increasing soil salinization is causing yield reductions worldwide. Improving salt stress tolerance in wheat requires the elucidation of the mechanistic basis of plant response to this abiotic stress factor. Although several studies have been performed to analyze wheat adaptation to salt stress, there are still some gaps to fully understand the molecular mechanisms from initial signal perception to the onset of responsive tolerance pathways. The main objective of this study is to exploit the dynamic salt stress transcriptome in underlying QTL regions to uncover candidate genes controlling salt stress tolerance in bread wheat. The massive analysis of 3′-ends sequencing protocol was used to analyze leave samples at osmotic and ionic phases. Afterward, stress-responsive genes overlapping QTL for salt stress-related traits in two mapping populations were identified. Results Among the over-represented salt-responsive gene categories, the early up-regulation of calcium-binding and cell wall synthesis genes found in the tolerant genotype are presumably strategies to cope with the salt-related osmotic stress. On the other hand, the down-regulation of photosynthesis-related and calcium-binding genes, and the increased oxidative stress response in the susceptible genotype are linked with the greater photosynthesis inhibition at the osmotic phase. The specific up-regulation of some ABC transporters and Na+/Ca2+ exchangers in the tolerant genotype at the ionic stage indicates their involvement in mechanisms of sodium exclusion and homeostasis. Moreover, genes related to protein synthesis and breakdown were identified at both stress phases. Based on the linkage disequilibrium blocks, salt-responsive genes within QTL intervals were identified as potential components operating in pathways leading to salt stress tolerance. Furthermore, this study conferred evidence of novel regions with transcription in bread wheat. Conclusion The dynamic transcriptome analysis allowed the comparison of osmotic and ionic phases of the salt stress response and gave insights into key molecular mechanisms involved in the salt stress adaptation of contrasting bread wheat genotypes. The leveraging of the highly contiguous chromosome-level reference genome sequence assembly facilitated the QTL dissection by targeting novel candidate genes for salt tolerance.

scholarly journals Overexpression of the DEAD-Box RNA Helicase Gene AtRH17 Confers Tolerance to Salt Stress in Arabidopsis

2018 ◽  
Vol 19 (12) ◽  
pp. 3777 ◽  
Author(s):  
Linh Nguyen ◽  
Hye-Yeon Seok ◽  
Dong-Hyuk Woo ◽  
Sun-Young Lee ◽  
Yong-Hwan Moon
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Rna Helicase ◽  
Activation Tagging ◽  
Salt Stress Tolerance ◽  
Salt Stress Response ◽  
Dead Box ◽  
Stress Responsive Genes

Plants adapt to abiotic stresses by complex mechanisms involving various stress-responsive genes. Here, we identified a DEAD-box RNA helicase (RH) gene, AtRH17, in Arabidopsis, involved in salt-stress responses using activation tagging, a useful technique for isolating novel stress-responsive genes. AT895, an activation tagging line, was more tolerant than wild type (WT) under NaCl treatment during germination and seedling development, and AtRH17 was activated in AT895. AtRH17 possesses nine well-conserved motifs of DEAD-box RHs, consisting of motifs Q, I, Ia, Ib, and II-VI. Although at least 12 orthologs of AtRH17 have been found in various plant species, no paralog occurs in Arabidopsis. AtRH17 protein is subcellularily localized in the nucleus. AtRH17-overexpressing transgenic plants (OXs) were more tolerant to high concentrations of NaCl and LiCl compared with WT, but no differences from WT were detected among seedlings exposed to mannitol and freezing treatments. Moreover, in the mature plant stage, AtRH17 OXs were also more tolerant to NaCl than WT, but not to drought, suggesting that AtRH17 is involved specifically in the salt-stress response. Notably, transcriptions of well-known abscisic acid (ABA)-dependent and ABA-independent stress-response genes were similar or lower in AtRH17 OXs than WT under salt-stress treatments. Taken together, our findings suggest that AtRH17, a nuclear DEAD-box RH protein, is involved in salt-stress tolerance, and that its overexpression confers salt-stress tolerance via a pathway other than the well-known ABA-dependent and ABA-independent pathways.

Progress in understanding salt stress response in plants using biotechnological tools

2021 ◽  
Vol 329 ◽  
pp. 180-191
Author(s):  
Ulkar İbrahimova ◽  
Pragati Kumari ◽  
Saurabh Yadav ◽  
Anshu Rastogi ◽  
Michal Antala ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response

scholarly journals Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 215 ◽  
Author(s):  
Guido Mastrobuoni ◽  
Susann Irgang ◽  
Matthias Pietzke ◽  
Heike E Aßmus ◽  
Markus Wenzel ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Chlamydomonas Reinhardtii ◽  
Salt Stress Response ◽  
Photosynthetic Organism

scholarly journals Interaction of SOS2 with Nucleoside Diphosphate Kinase 2 and Catalases Reveals a Point of Connection between Salt Stress and H2O2 Signaling in Arabidopsis thaliana

2007 ◽  
Vol 27 (22) ◽  
pp. 7771-7780 ◽  
Author(s):  
Paul E. Verslues ◽  
Giorgia Batelli ◽  
Stefania Grillo ◽  
Fernanda Agius ◽  
Yong-Sig Kim ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Stress Responses ◽  
Double Mutant ◽  
Nucleoside Diphosphate Kinase ◽  
Salt Resistance ◽  
Interacting Proteins ◽  
Salt Stress Response ◽  
Oxygen Signaling ◽  
Nucleoside Diphosphate Kinase 2

ABSTRACT SOS2, a class 3 sucrose-nonfermenting 1-related kinase, has emerged as an important mediator of salt stress response and stress signaling through its interactions with proteins involved in membrane transport and in regulation of stress responses. We have identified additional SOS2-interacting proteins that suggest a connection between SOS2 and reactive oxygen signaling. SOS2 was found to interact with the H2O2 signaling protein nucleoside diphosphate kinase 2 (NDPK2) and to inhibit its autophosphorylation activity. A sos2-2 ndpk2 double mutant was more salt sensitive than a sos2-2 single mutant, suggesting that NDPK2 and H2O2 are involved in salt resistance. However, the double mutant did not hyperaccumulate H2O2 in response to salt stress, suggesting that it is altered signaling rather than H2O2 toxicity alone that is responsible for the increased salt sensitivity of the sos2-2 ndpk2 double mutant. SOS2 was also found to interact with catalase 2 (CAT2) and CAT3, further connecting SOS2 to H2O2 metabolism and signaling. The interaction of SOS2 with both NDPK2 and CATs reveals a point of cross talk between salt stress response and other signaling factors including H2O2.

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