scholarly journals Soybean GmMYB133 Inhibits Hypocotyl Elongation and Confers Salt Tolerance in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Binghui Shan ◽  
Wei Wang ◽  
Jinfeng Cao ◽  
Siqi Xia ◽  
Ruihua Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Agronomic Traits ◽  
Salt Stress Tolerance ◽  
Developmental Defects ◽  
Functional Roles ◽  
Regulatory Module

REVEILLE (RVE) genes generally act as core circadian oscillators to regulate multiple developmental events and stress responses in plants. It is of importance to document their roles in crops for utilizing them to improve agronomic traits. Soybean is one of the most important crops worldwide. However, the knowledge regarding the functional roles of RVEs is extremely limited in soybean. In this study, the soybean gene GmMYB133 was shown to be homologous to the RVE8 clade genes of Arabidopsis. GmMYB133 displayed a non-rhythmical but salt-inducible expression pattern. Like AtRVE8, overexpression of GmMYB133 in Arabidopsis led to developmental defects such as short hypocotyl and late flowering. Seven light-responsive or auxin-associated genes including AtPIF4 were transcriptionally depressed by GmMYB133, suggesting that GmMYB133 might negatively regulate plant growth. Noticeably, the overexpression of GmMYB133 in Arabidopsis promoted seed germination and plant growth under salt stress, and the contents of chlorophylls and malondialdehyde (MDA) were also enhanced and decreased, respectively. Consistently, the expressions of four positive regulators responsive to salt tolerance were remarkably elevated by GmMYB133 overexpression, indicating that GmMYB133 might confer salt stress tolerance. Further observation showed that GmMYB133 overexpression perturbed the clock rhythm of AtPRR5, and yeast one-hybrid assay indicated that GmMYB133 could bind to the AtPRR5 promoter. Moreover, the retrieved ChIP-Seq data showed that AtPRR5 could directly target five clients including AtPIF4. Thus, a regulatory module GmMYB133-PRR5-PIF4 was proposed to regulate plant growth and salt stress tolerance. These findings laid a foundation to further address the functional roles of GmMYB133 and its regulatory mechanisms in soybean.

scholarly journals Melatonin: A Small Molecule but Important for Salt Stress Tolerance in Plants

2019 ◽  
Vol 20 (3) ◽  
pp. 709 ◽  
Author(s):  
Haoshuang Zhan ◽  
Xiaojun Nie ◽  
Ting Zhang ◽  
Shuang Li ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Ion Homeostasis ◽  
Free Radical Scavenger ◽  
Limiting Factors ◽  
Polyamine Metabolism ◽  
Antioxidant Systems ◽  
Radical Scavenger ◽  
Salt Stress Tolerance

Salt stress is one of the most serious limiting factors in worldwide agricultural production, resulting in huge annual yield loss. Since 1995, melatonin (N-acetyl-5-methoxytryptamine)—an ancient multi-functional molecule in eukaryotes and prokaryotes—has been extensively validated as a regulator of plant growth and development, as well as various stress responses, especially its crucial role in plant salt tolerance. Salt stress and exogenous melatonin lead to an increase in endogenous melatonin levels, partly via the phyto-melatonin receptor CAND2/PMTR1. Melatonin plays important roles, as a free radical scavenger and antioxidant, in the improvement of antioxidant systems under salt stress. These functions improve photosynthesis, ion homeostasis, and activate a series of downstream signals, such as hormones, nitric oxide (NO) and polyamine metabolism. Melatonin also regulates gene expression responses to salt stress. In this study, we review recent literature and summarize the regulatory roles and signaling networks involving melatonin in response to salt stress in plants. We also discuss genes and gene families involved in the melatonin-mediated salt stress tolerance.

scholarly journals Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis

2020 ◽  
Vol 21 (5) ◽  
pp. 1595 ◽  
Author(s):  
Hye-Yeon Seok ◽  
Linh Vu Nguyen ◽  
Doai Van Nguyen ◽  
Sun-Young Lee ◽  
Yong-Hwan Moon
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Rna Helicase ◽  
Rna Seq ◽  
Salt Stress Tolerance ◽  
Salt Stress Condition ◽  
Dead Box ◽  
Helicase Gene ◽  
Upregulated Genes

Previously, we reported that overexpression of AtRH17, an Arabidopsis DEAD-box RNA helicase gene, confers salt stress-tolerance via a pathway other than the well-known salt stress-responsive pathways. To decipher the salt stress-responsive pathway in AtRH17-overexpressing transgenic plants (OXs), we performed RNA-Sequencing and identified 397 differentially expressed genes between wild type (WT) and AtRH17 OXs. Among them, 286 genes were upregulated and 111 genes were downregulated in AtRH17 OXs relative to WT. Gene ontology annotation enrichment and KEGG pathway analysis showed that the 397 upregulated and downregulated genes are involved in various biological functions including secretion, signaling, detoxification, metabolic pathways, catabolic pathways, and biosynthesis of secondary metabolites as well as in stress responses. Genevestigator analysis of the upregulated genes showed that nine genes, namely, LEA4-5, GSTF6, DIN2/BGLU30, TSPO, GSTF7, LEA18, HAI1, ABR, and LTI30, were upregulated in Arabidopsis under salt, osmotic, and drought stress conditions. In particular, the expression levels of LEA4-5, TSPO, and ABR were higher in AtRH17 OXs than in WT under salt stress condition. Taken together, our results suggest that a high AtRH17 expression confers salt stress-tolerance through a novel salt stress-responsive pathway involving nine genes, other than the well-known ABA-dependent and ABA-independent pathways.

scholarly journals Foliar Application of 24-Epibrassinolide Improved Salt Stress Tolerance of Perennial Ryegrass

HortScience ◽  
2015 ◽  
Vol 50 (10) ◽  
pp. 1518-1523 ◽  
Author(s):  
Shanshan Sun ◽  
Mengying An ◽  
Liebao Han ◽  
Shuxia Yin
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Tolerance ◽  
Perennial Ryegrass ◽  
Foliar Application ◽  
Soluble Sugar ◽  
Salt Stress Tolerance ◽  
Salt Stress Condition ◽  
Relative Water ◽  
Antioxidant Defense Systems

Perennial ryegrass (Lolium perenne L.) is a widely used turfgrass. In this study, the effect of exogenously applied 24-epibrassinolide (EBR) on salt stress tolerance of perennial ryegrass was investigated. The results indicated that pretreatment with four concentrations of EBR (0, 0.1, 10, 1000 nM) improved salt tolerance of perennial ryegrass. Exogenous EBR treatment decreased electrolyte leakage (EL), malondialdehyde (MDA), and H2O2 contents and enhanced the leaf relative water content (RWC), proline, soluble sugar, and soluble protein content under salt stress condition. Meanwhile, EBR reduced the accumulation of Na+ and increased K+, Ca2+, and Mg2+ contents in leaves after salt treatment. Moreover, EBR pretreatment also increased superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) activity, as well as ascorbic acid (AsA) and glutathione contents. These results suggested that EBR improved salt tolerance by enhancing osmotic adjustment and antioxidant defense systems in perennial ryegrass.

scholarly journals Temporal salt stress-induced transcriptome alterations and regulatory mechanisms revealed by PacBio long-reads RNA sequencing in Gossypium hirsutum

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Delong Wang ◽  
Xuke Lu ◽  
Xiugui Chen ◽  
Shuai Wang ◽  
Junjuan Wang ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Gossypium Hirsutum ◽  
Stress Tolerance ◽  
Economic Losses ◽  
Salt Tolerant ◽  
Salt Stress Tolerance ◽  
Long Reads

Abstract Background Cotton (Gossypium hirsutum) is considered a fairly salt tolerant crop however, salinity can still cause significant economic losses by affecting the yield and deteriorating the fiber quality. We studied a salt-tolerant upland cotton cultivar under temporal salt stress to unfold the salt tolerance molecular mechanisms. Biochemical response to salt stress (400 mM) was measured at 0 h, 3 h, 12 h, 24 h and 48 h post stress intervals and single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) combined with the unique molecular identifiers approach was used to identify differentially expressed genes (DEG). Results Antioxidant enzymes including, catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) were found significantly induced under temporal salt stress, suggesting that reactive oxygen species scavenging antioxidant machinery is an essential component of salt tolerance mechanism in cotton. We identified a wealth of novel transcripts based on the PacBio long reads sequencing approach. Prolonged salt stress duration induces high number of DEGs. Significant numbers of DEGs were found under key terms related to stress pathways such as “response to oxidative stress”, “response to salt stress”, “response to water deprivation”, “cation transport”, “metal ion transport”, “superoxide dismutase”, and “reductase”. Key DEGs related to hormone (abscisic acid, ethylene and jasmonic acid) biosynthesis, ion homeostasis (CBL-interacting serine/threonine-protein kinase genes, calcium-binding proteins, potassium transporter genes, potassium channel genes, sodium/hydrogen exchanger or antiporter genes), antioxidant activity (POD, SOD, CAT, glutathione reductase), transcription factors (myeloblastosis, WRKY, Apetala 2) and cell wall modification were found highly active in response to salt stress in cotton. Expression fold change of these DEGs showed both positive and negative responses, highlighting the complex nature of salt stress tolerance mechanisms in cotton. Conclusion Collectively, this study provides a good insight into the regulatory mechanism under salt stress in cotton and lays the foundation for further improvement of salt stress tolerance.

scholarly journals Down-regulation ofGIGANTEA-likegenes increases plant growth and salt stress tolerance in poplar

2016 ◽  
Vol 15 (3) ◽  
pp. 331-343 ◽  
Author(s):  
Qingbo Ke ◽  
Ho Soo Kim ◽  
Zhi Wang ◽  
Chang Yoon Ji ◽  
Jae Cheol Jeong ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Salt Stress Tolerance ◽  
Down Regulation

scholarly journals Leucine-rich repeat extensin proteins regulate plant salt tolerance in Arabidopsis

2018 ◽  
Vol 115 (51) ◽  
pp. 13123-13128 ◽  
Author(s):  
Chunzhao Zhao ◽  
Omar Zayed ◽  
Zheping Yu ◽  
Wei Jiang ◽  
Peipei Zhu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Leucine Rich Repeat ◽  
Salt Stress Tolerance ◽  
Triple Mutant ◽  
Underlying Mechanisms ◽  
Plant Salt Tolerance ◽  
Rapid Alkalinization Factor

The perception and relay of cell-wall signals are critical for plants to regulate growth and stress responses, but the underlying mechanisms are poorly understood. We found that the cell-wall leucine-rich repeat extensins (LRX) 3/4/5 are critical for plant salt tolerance in Arabidopsis. The LRXs physically associate with the RAPID ALKALINIZATION FACTOR (RALF) peptides RALF22/23, which in turn interact with the plasma membrane-localized receptor-like protein kinase FERONIA (FER). The lrx345 triple mutant as well as fer mutant plants display retarded growth and salt hypersensitivity, which are mimicked by overexpression of RALF22/23. Salt stress promotes S1P protease-dependent release of mature RALF22 peptides. Treatment of roots with mature RALF22/23 peptides or salt stress causes the internalization of FER. Our results suggest that the LRXs, RALFs, and FER function as a module to transduce cell-wall signals to regulate plant growth and salt stress tolerance.

scholarly journals Genetic regulation of salt stress tolerance revealed by RNA-Seq in cotton diploid wild species, Gossypium davidsonii

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Feng Zhang ◽  
Guozhong Zhu ◽  
Lei Du ◽  
Xiaoguang Shang ◽  
Chaoze Cheng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Genetic Regulation ◽  
Ion Homeostasis ◽  
Rna Seq ◽  
Salt Stress Tolerance ◽  
Crop Resistance ◽  
Cotton Species ◽  
Diploid Cotton

Abstract Cotton is an economically important crop throughout the world and is a pioneer crop in salt stress tolerance research. Investigation of the genetic regulation of salinity tolerance will provide information for salt stress-resistant breeding. Here, we employed next-generation RNA-Seq technology to elucidate the salt-tolerant mechanisms in cotton using the diploid cotton species Gossypium davidsonii which has superior stress tolerance. A total of 4744 and 5337 differentially expressed genes (DEGs) were found to be involved in salt stress tolerance in roots and leaves, respectively. Gene function annotation elucidated salt overly sensitive (SOS) and reactive oxygen species (ROS) signaling pathways. Furthermore, we found that photosynthesis pathways and metabolism play important roles in ion homeostasis and oxidation balance. Moreover, our studies revealed that alternative splicing also contributes to salt-stress responses at the posttranscriptional level, implying its functional role in response to salinity stress. This study not only provides a valuable resource for understanding the genetic control of salt stress in cotton, but also lays a substantial foundation for the genetic improvement of crop resistance to salt stress.

The R2R3-type MYB gene OsMYB91 has a function in coordinating plant growth and salt stress tolerance in rice

Plant Science ◽  
2015 ◽  
Vol 236 ◽  
pp. 146-156 ◽  
Author(s):  
Ning Zhu ◽  
Saifeng Cheng ◽  
Xiaoyun Liu ◽  
Hao Du ◽  
Mingqiu Dai ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Salt Stress Tolerance ◽  
Myb Gene

scholarly journals PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway

2018 ◽  
Vol 19 (9) ◽  
pp. 2609 ◽  
Author(s):  
Ping Li ◽  
Hong Yang ◽  
Gaojing Liu ◽  
Wenzhang Ma ◽  
Chuanhong Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Physcomitrella Patens ◽  
Stress Conditions ◽  
Negative Regulator ◽  
Salt Stress Response ◽  
Functional Roles ◽  
Related Pathway

Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss.

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