Simultaneous detection of roxithromycin and dopamine using a sensor platform based on poly(sulfosalicylic acid) and its application in human serum studies

2014 ◽  
Vol 6 (10) ◽  
pp. 3316-3321 ◽  
Author(s):  
Xin Zhang ◽  
Shuqing Gu ◽  
Yaping Ding
Keyword(s):  
Detection Limit ◽  
Human Serum ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Simultaneous Detection ◽  
Sensitive Detection ◽  
Serum Samples ◽  
Sulfosalicylic Acid ◽  
Sensor Platform

An electrochemical sensor based on poly(sulfosalicylic acid) was fabricated for individual and simultaneous determination of roxithromycin and dopamine with a sensitive detection limit and a satisfactory detection result using human serum samples.

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

scholarly journals MnO2@Reduced Graphene Oxide Nanocomposite-Based Electrochemical Sensor for the Simultaneous Determination of Trace Cd(II), Zn(II) and Cu(II) in Water Samples

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 517
Author(s):  
Siyamthanda Hope Mnyipika ◽  
Tshimangadzo Saddam Munonde ◽  
Philiswa Nosizo Nomngongo
Keyword(s):  
Heavy Metals ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Rapid Detection ◽  
Electrochemical Sensors ◽  
Simultaneous Detection ◽  
Cyclic Voltammograms ◽  
Current Response ◽  
Mno2 Nanoparticles

The rapid detection of trace metals is one of the most important aspect in achieving environmental monitoring and protection. Electrochemical sensors remain a key solution for rapid detection of heavy metals in environmental water matrices. This paper reports the fabrication of an electrochemical sensor obtained by the simultaneous electrodeposition of MnO2 nanoparticles and RGO nanosheets on the surface of a glassy carbon electrode. The successful electrodeposition was confirmed by the enhanced current response on the cyclic voltammograms. The XRD, HR-SEM/EDX, TEM, FTIR, and BET characterization confirmed the successful synthesis of MnO2 nanoparticles, RGO nanosheets, and MnO2@RGO nanocomposite. The electrochemical studies results revealed that MnO2@RGO@GCE nanocomposite considerably improved the current response on the detection of Zn(II), Cd(II) and Cu(II) ions in surface water. These remarkable improvements were due to the interaction between MnO2 nanomaterials and RGO nanosheets. Moreover, the modified sensor electrode portrayed high sensitivity, reproducibility, and stability on the simultaneous determination of Zn(II), Cd(II), and Cu(II) ions. The detection limits of (S/N = 3) ranged from 0.002–0.015 μg L−1 for the simultaneous detection of Zn(II), Cd(II), and Cu(II) ions. The results show that MnO2@RGO nanocomposite can be successfully used for the early detection of heavy metals with higher sensitivity in water sample analysis.

Simultaneous determination of ascorbic and uric acids and dopamine in human serum samples using three-way calibration with data from square wave voltammetry

2016 ◽  
Vol 129 ◽  
pp. 205-212 ◽  
Author(s):  
Adrian Marcelo Granero ◽  
Gastón Darío Pierini ◽  
Sebastián Noel Robledo ◽  
María Susana Di Nezio ◽  
Héctor Fernández ◽  
...  
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Square Wave Voltammetry ◽  
Serum Samples ◽  
Square Wave ◽  
Human Serum Samples ◽  
Wave Voltammetry

scholarly journals Direct Injection Liquid Chromatographic Technique for Simultaneous Determination of Two Antihistaminic Drugs and Their Main Metabolites in Serum

2007 ◽  
Vol 90 (2) ◽  
pp. 384-390 ◽  
Author(s):  
Samy Emara ◽  
Alaa El-Gindy ◽  
Mostafa K Mesbah ◽  
Ghada M Hadad
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Chromatographic Technique ◽  
Simple Liquid ◽  
Good Precision ◽  
Active Metabolites ◽  
Serum Samples ◽  
Antihistaminic Drugs ◽  
Liquid Chromatographic

Abstract A very simple liquid chromatographic technique was developed and validated for the simultaneous determination of 2 antihistaminic drugs, loratadine (LT) and terfenadine (TR), and their major active metabolites, desloratadine (DL) and fexofenadine (FX), respectively, in human serum. LT, DL, TR, and FX from directly injected serum samples were enriched on a protein-coated RP8 silica precolumn (10 4.6 mm id) while serum constituents, such as proteins and salts, were eluted to waste. Using an online column-switching system, the drugs and their metabolites were quantitatively transferred and separated on a second analytical column (Shim-pack 5 μm particle size cyanopropyl, 250 × 4.6 mm id) followed by ultraviolet detection at 243 nm for LT and DL and 220 nm for TR and FX. Very good precision, accuracy, and linearity were obtained over the range of 101000 ng/mL for LT and DL, 10500 ng/mL for TR, and 103000 ng/mL for FX in human serum. High extraction recoveries from serum ranging from 96.03 to 98.19, 95.44 to 97.26, 95.61 to 98.17, and 95.60 to 97.89 for LT, DL, TR, and FX, respectively, were obtained.

A Rapid and Sensitive HPLC-FLD Method for the Determination of Retinol and Vitamin E Isomers in Human Serum

2019 ◽  
Vol 15 (7) ◽  
pp. 745-752
Author(s):  
Yi Yang ◽  
Dan Lu ◽  
Danni Yang ◽  
Shuo Yin ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Vitamin E ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
High Performance ◽  
Correlation Coefficients ◽  
Serum Samples ◽  
Rapid Separation ◽  
Relative Standard

Background: Retinol and vitamin E are fat-soluble vitamins crucial for human health, yet their isomers’ distributions in the human body are still known roughly. In order to figure out the physical condition and evaluate the nutritional status of an individual, it is imperative to analyze retinol and VE isomers in human serum. Objective: This work aims to establish a rapid and simple high-performance liquid chromatography with fluorescence detection for simultaneous determination of retinol and vitamin E isomers in human serum. Methods: Separation was accomplished on a common C18 column thermostated at 25 oC, using a simple isocratic elution program of methanol/acetonitrile (85:15, v/v) at a flow rate of 1.0 mL/min. Fluorescence detection was operated using excitation/emission wavelengths of 329 nm/472 nm for retinol and 294 nm/338 nm for VE isomers, respectively. Results: Rapid separation was achieved within 13 min. Linear ranges of the method were 0.020-50.0 µg/mL, with correlation coefficients greater than 0.999. Detection limits and the quantification limits were 0.001-0.004 µg/mL and 0.003- 0.013 µg/mL, respectively. Mean recoveries were 84.1%- 98.2%, with intra-day and inter-day relative standard deviations less than 12.3% and 13.6%, respectively. This method has been applied to the simultaneous determination of retinol and 8 VE isomers in human serum samples with satisfactory results. Conclusion: A rapid, simple and robust method was developed for routine analysis of retinol and eight vitamin E isomers in human serum, providing a useful tool for clinical diagnosis and nutritional evaluation.

A Novel Electrochemical Sensor for the Simultaneous Determination of Fat-Soluble Vitamins Using a Screen-Printed Graphene/Nafion Electrode

2018 ◽  
Vol 777 ◽  
pp. 597-601 ◽  
Author(s):  
Jeerakit Thangphatthanarungruang ◽  
Aroonsri Ngamaroonchote ◽  
Rawiwan Laocharoensuk ◽  
Chuleekorn Chotsuwan ◽  
Weena Siangproh
Keyword(s):  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Sodium Perchlorate ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Morphological Characterization ◽  
Quiet Time ◽  
Fat Soluble Vitamins ◽  
Screen Printed

In this work, a novel electrochemical sensor was proposed for the simultaneous determination of fat-soluble vitamins (A, D, E, K) using a screen-printed graphene/Nafion electrode (SPGNE). The scanning electron microscopy was used for morphological characterization of the electrode surface. The electrochemical behaviors of fat-soluble vitamins have been studied in a mixture of ethanol and sodium perchlorate monohydrate using square-wave voltammetry (SWV). The results obtained indicated that the oxidation peak of each fat-soluble vitamin appeared at different potentials leading to the possibility for the simultaneous detection. The influences of experimental parameters such as the effects of proportions of ethanol, potential increment, amplitude, frequency and quiet time were examined. Under the optimized conditions, the linearity between oxidative currents and concentrations of fat-soluble vitamins ranged from 0.1 μg mL-1 to 5 μg mL-1 for vitamin A, 0.08 μg mL-1 to 5 μg mL-1 for vitamin D and E, and 0.2 μg mL-1 to 1.6 μg mL-1 for total vitamin K, with the limits of detection of 0.018, 0.013, 0.012 and 0.004 μg mL-1, respectively. These developed sensors provide high sensitivity in detection and offer high potential to apply them for the simultaneous determination of fat-soluble vitamins in dietary supplements.

Glycation ratio determination through simultaneous detection of human serum albumin and glycated albumin on an advanced lateral flow immunoassay sensor

Lab on a Chip ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 844-851 ◽  
Author(s):  
Hangil Ki ◽  
Jusung Oh ◽  
Gyeo-Re Han ◽  
Min-Gon Kim
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Glycated Albumin ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Ratio Determination

We developed two advanced lateral flow immunoassays (LFIAs) for the simultaneous determination of total human serum albumin and glycated albumin concentrations with wide detection ranges and improved glycated albumin selectivity.

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