Quantification of CP4 EPSPS in genetically modified Nicotiana tabacum leaves by LC-MS/MS with18O-labelling

Soybean MON 89788 expresses the cp4 epsps gene from the plant pathogenic bacterium Agrobacterium tumefaciens (Rhizobium radiobacter) sp. strain CP4. The encoded enzyme 5enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein confers tolerance to the active herbicidal substance glyphosate. Updated bioinformatics analyses of the inserted DNA and flanking sequences in soybean MON 89788 have not indicated a potential production of putative harmful proteins or polypeptides caused by the genetic modification. Genomic stability of the functional insert and consistent expression of the cp4 epsps gene, have been shown over several generations of soybean MON 89788. With the exception of the intended changes caused by the trans-genetically introduced trait, data from several field trials performed in USA and Argentina show that soybean MON 89788 is compositionally, morphologically and agronomically equivalent to its conventional counterpart and other commercial soybean varieties. A sub-chronic feeding study with rats, as well as a nutritional assessment trial with broilers has not revealed adverse effects of soybean MON 89788. These studies indicate that soybean MON 89788 is nutritionally equivalent to, and as safe as conventional soybean varieties. The CP4 EPSPS protein produced in soybean MON 89788 does not show sequence resemblance to known toxins or IgE-dependent allergens, nor has it been reported to cause IgE-mediated allergic reactions. Soybean is not cultivated in Norway, and there are no cross-compatible wild or weedy relatives of soybean in Europe. Based on current knowledge, the VKM GMO Panel concludes that with the intended usage, there are no discernible safety concerns associated with soybean MON 89788 regarding human or animal health or to the environment in Norway.

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The pros and cons of GM crops

Functional Plant Biology ◽

10.1071/fp17031 ◽

2018 ◽

Vol 45 (3) ◽

pp. 297 ◽

Cited By ~ 2

Author(s):

Jennifer A. Thomson

Keyword(s):

Developing Countries ◽

Genetically Modified ◽

Environmental Benefits ◽

Gm Crops ◽

Foreseeable Future ◽

Main Ingredient ◽

The World ◽

Herbicide Tolerant ◽

Pros And Cons ◽

Insect Resistant

The year 2015 marked the 20th year of the commercialisation of genetically modified (GM) crops. During the period from 1996 to 2014, the global hectarage of these crops increased 100-fold, making it the fastest adopted crop technology in recent times. The overall economic gains from these crops have been estimated to be USD133.4 billion over the period from 1996 to 2013, and have been divided roughly 50% each to farmers in developed and developing countries. The environmental benefits include contributing to the practice of minimal till agriculture and a decrease in the use of pesticides. But what are the downsides of this technology? In this review I look at some of the problems related to weeds becoming resistant to glyphosate (the main ingredient that is used on herbicide tolerant crops), how these can be overcome and whether glyphosate can cause cancer. I also discuss the problem of insects becoming resistant to the toxins that are used in insect resistant crops and how these are being addressed. I look at what scientists around the world are saying on this topic and then consider GM crops that are in the pipeline of benefit to developing countries and whether any of these are likely to be commercialised in the foreseeable future.

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Expression patterns of cp4-epsps gene in diverse transgenic Saccharum officinarum L. genotypes

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-019-00650-y ◽

2019 ◽

Vol 25 (3) ◽

pp. 779-786

Author(s):

Muhammad Imran ◽

Andre Luiz Barboza ◽

Shaheen Asad ◽

Zafar M. Khalid ◽

Zahid Mukhtar

Keyword(s):

Expression Patterns ◽

Saccharum Officinarum ◽

Epsps Gene ◽

Cp4 Epsps Gene ◽

Cp4 Epsps

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A necessity of introduction of the system of registration of GMO is for Ukraine

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9428 ◽

2019 ◽

Vol 21 (94) ◽

pp. 152-156

Author(s):

B. I. Nazar ◽

H. V. Kushnir ◽

H. I. Boiko ◽

S. D. Murska

Keyword(s):

Genetically Modified ◽

The State ◽

Feed Additives ◽

Chain Reaction ◽

Genetically Modified Soybean ◽

Epsps Gene ◽

Polymerase Chain ◽

State Registration ◽

Cp4 Epsps Gene ◽

Herbicide Glyphosate

The article presents the main methodological approaches and criteria for the necessity of development and introduction of the State Registration of GMOs sources in Ukraine. The list of analytical researches that are to be carried out at the registration of the GMO source, expert evaluation of GMO identification methods, reproduction of detection methods, identification and quantification of GMOs are given. Submitted and analyzed legislative documents regulating the procedure for registration and control of GMO circulation in the countries of the European Union and Ukraine. DNDKI of veterinary preparations and fodder supplements on the tasks of the State Committee of Veterinary Medicine of Ukraine during 2010-2018 a number of normative documents were developed regarding procedures related to the use and circulation of feed, feed additives, premixes and veterinary preparations that are composed, containing or made using genetically modified sources in Ukraine: the procedure for state registration, the procedure for carrying out the state veterinary and sanitary examination of GMOs for sources of forage feed additives and veterinary preparations ativ plan state monitoring GMO sources of feed, feed additives, premixes for livestock and poultry. The procedure determines the procedure for state registration of GMOs for sources of feed, feed additives and veterinary preparations containing genetically modified organisms or obtained from their use. It is envisaged that the state register of GMO sources is conducted in the form approved by the State Service of Ukraine for Food Safety and Consumer Protection. On the basis of the analysis, a draft list of feeds, feed materials, protein-vitamin concentrates and premixes that have to be monitored for GMO content has been developed. This project list includes soy, corn, rape and products of their processing, fodder, feed mixes, feed concentrates, etc. In the SSRCI of veterinary drugs and supplements, in 2018, the “Guidelines for identifying the GM soya line (Soybean MON40-3-2) GTS 40-3-2” were developed and approved. “Methodological recommendations are intended to identify the GM soya line (Soybean MON40-3 -2) GTS 40-3-2 (Genetically modified soybean that contains – genetically modified soybean containing (cp4 epsps gene inserted to confer tolerance to herbicide glyphosate) cp4 epsps gene inserted to provide resistance to glyphosate herbicide (glyphosate) in plant material, feed and food products by polymerase chain reaction in real time (PCR-RF )”. The qualitative determination of GMOs is based on the discovery of genetically modified (GM) targeted regulatory sequences of the 35S Cauliflower Mosaic Virus (CaMV) promoter or FMV promoter and/or the NOS (T-NOS) T1 terminator of Agrobacterium tumefaciens plasmids that are introduced into the genetic constructs of GM based on the use of the multiplex polymerase chain reaction method to obtain real-time research results – PCR RF.

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