Fish oil diet modulates epididymal and inguinal adipocyte metabolism in mice

2016 ◽  
Vol 7 (3) ◽  
pp. 1468-1476 ◽  
Author(s):  
Thereza Cristina Lonzetti Bargut ◽  
Vanessa Souza-Mello ◽  
Carlos Alberto Mandarim-de-Lacerda ◽  
Marcia Barbosa Aguila
Keyword(s):  
Adipose Tissue ◽  
Fish Oil ◽  
White Adipose Tissue ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets ◽  
Fish Oil Diet ◽  
The Impact

We aimed to investigate the impact of different high-fat diets containing fish oil on adiposity and white adipose tissue (WAT) function in mice, comparing the effects on epididymal (eWAT) and subcutaneous (sWAT) depots.

scholarly journals The intake of high-fat diets induces the acquisition of brown adipocyte gene expression features in white adipose tissue

2015 ◽  
Vol 39 (11) ◽  
pp. 1619-1629 ◽  
Author(s):  
E García-Ruiz ◽  
B Reynés ◽  
R Díaz-Rúa ◽  
E Ceresi ◽  
P Oliver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Brown Adipocyte ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

scholarly journals Dietary n-3 fatty acids affect mRNA level of brown adipose tissue uncoupling protein 1, and white adipose tissue leptin and glucose transporter 4 in the rat

2000 ◽  
Vol 84 (2) ◽  
pp. 175-184 ◽  
Author(s):  
Yoko Takahashi ◽  
Takashi Ide
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Mrna Level ◽  
Safflower Oil ◽  
High Fat ◽  
Low Fat ◽  
Low Fat Diet ◽  
Brown Adipose ◽  
High Fat Diets ◽  
Fat Diets

We examined the effect of dietary fats rich in n-3 polyunsaturated fatty acids (PUFA) on mRNA levels in white and brown adipose tissues in rats. Four groups of rats were fed on a low-fat diet (20 g safflower oil/kg) or a high-fat diet (200 g/kg) containing safflower oil, which is rich in n-6 PUFA (linoleic acid), or perilla (α-linolenic acid) or fish oil (eicosapentaenoic and docosahexaenoic acids), both of which are rich in n-3 PUFA, for 21 d. Energy intake was higher in rats fed on a high-safflower-oil diet than in those fed on low-fat or high-fish-oil diet, but no other significant differences were detected among the groups. Perirenal white adipose tissue weight was higher and epididymal white adipose tissue weight tended to be higher in rats fed on a high-safflower-oil diet than in those fed on a low-fat diet. However, high-fat diets rich in n-3 PUFA, compared to a low-fat diet, did not increase the white adipose tissue mass. High-fat diets relative to a low-fat diet increased brown adipose tissue uncoupling protein 1 mRNA level. The increases were greater with fats rich in n-3 PUFA than with n-6 PUFA. A high-safflower-oil diet, compared to a low-fat diet, doubled the leptin mRNA level in white adipose tissue. However, high-fat diets rich in n-3 PUFA failed to increase it. Compared to a low-fat diet, high-fat diets down-regulated the glucose transporter 4 mRNA level in white adipose tissue. However, the decreases were attenuated with high-fat diets rich in n-3 PUFA. It is suggested that the alterations in gene expression in adipose tissue contribute to the physiological activities of n-3 PUFA in preventing body fat accumulation and in regulating glucose metabolism in rats.

scholarly journals Oil composition of high-fat diet affects metabolic inflammation differently in connection with endotoxin receptors in mice

2012 ◽  
Vol 302 (3) ◽  
pp. E374-E386 ◽  
Author(s):  
Fabienne Laugerette ◽  
Jean-Pierre Furet ◽  
Cyrille Debard ◽  
Patricia Daira ◽  
Emmanuelle Loizon ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Palm Oil ◽  
Rapeseed Oil ◽  
Low Grade ◽  
Oil Composition ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets ◽  
The Impact ◽  
Low Grade Inflammation

Low-grade inflammation observed in obesity is a risk factor for cardiovascular disease. Recent studies revealed that this would be linked to gut-derived endotoxemia during fat digestion in high-fat diets, but nothing is known about the effect of lipid composition. The study was designed to test the impact of oil composition of high-fat diets on endotoxin metabolism and inflammation in mice. C57/Bl6 mice were fed for 8 wk with chow or isocaloric isolipidic diets enriched with oils differing in fatty acid composition: milk fat, palm oil, rapeseed oil, or sunflower oil. In vitro, adipocytes (3T3-L1) were stimulated or not with lipopolysaccharide (LPS; endotoxin) and incubated with different fatty acids. In mice, the palm group presented the highest level of IL-6 in plasma ( P < 0.01) together with the highest expression in adipose tissue of IL-1β and of LPS-sensing TLR4 and CD14 ( P < 0.05). The higher inflammation in the palm group was correlated with a greater ratio of LPS-binding protein (LBP)/sCD14 in plasma ( P < 0.05). The rapeseed group resulted in higher sCD14 than the palm group, which was associated with lower inflammation in both plasma and adipose tissue despite higher plasma endotoxemia. Taken together, our results reveal that the palm oil-based diet resulted in the most active transport of LPS toward tissues via high LBP and low sCD14 and the greatest inflammatory outcomes. In contrast, a rapeseed oil-based diet seemed to result in an endotoxin metabolism driven toward less inflammatory pathways. This shows that dietary fat composition can contribute to modulate the onset of low-grade inflammation through the quality of endotoxin receptors.

The impact of High Fat Diet on bone: potential mechanisms

2021 ◽  
Author(s):  
Qiao Jie ◽  
Yue-Zhong Ren ◽  
Yi-wen Wu
Keyword(s):  
Total Energy ◽  
High Fat Diet ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets ◽  
The Impact

High-fat diets(HFD)are defined as lipids accounting for exceeded 30% of total energy in-take, and current research is mostly 45% and 60%. With a view of the tendency that patients who...

Effects of high fat diets and supplemental tart cherry and fish oil on obesity and type 2 diabetes in male and female C57BL/6J and TALLYHO/Jng mice

2021 ◽  
pp. 108644
Author(s):  
Jacaline K. Parkman ◽  
Kristiana Sklioutovskaya-Lopez ◽  
Kalhara R. Menikdiwela ◽  
Logan Freeman ◽  
Naima Moustaid-Moussa ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Fish Oil ◽  
Tart Cherry ◽  
High Fat ◽  
Male And Female ◽  
High Fat Diets ◽  
Fat Diets

scholarly journals The triglyceride synthesis enzymes DGAT1 and DGAT2 have distinct and overlapping functions in adipocytes

2019 ◽  
Vol 60 (6) ◽  
pp. 1112-1120 ◽  
Author(s):  
Chandramohan Chitraju ◽  
Tobias C. Walther ◽  
Robert V. Farese
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Convergent Evolution ◽  
Metabolic Energy ◽  
Chow Diet ◽  
High Fat ◽  
Functional Differences ◽  
High Fat Diets ◽  
Fat Stores ◽  
Fat Diets

Mammals store metabolic energy as triacylglycerols (TGs) in adipose tissue. TG synthesis is catalyzed by the evolutionarily unrelated acyl-CoA:diacylglycerol acyltransferase (DGAT) enzymes DGAT1 and DGAT2, which catalyze the same reaction and account for nearly all TG synthesis. The reasons for their convergent evolution to synthesize TGs remain unclear. Mice lacking DGAT1 are viable with reduced fat stores of TGs, whereas DGAT2 KO mice die postnatally just after birth with >90% reduction of TGs, suggesting that DGAT2 is the predominant enzyme for TG storage. To better understand the functional differences between the DGATs, we studied mice fed chow or high-fat diets lacking either enzyme in adipose tissue. Unexpectedly, mice lacking DGAT2 in adipocytes have normal TG storage and glucose metabolism on regular or high-fat diets, indicating DGAT2 is not essential for fat storage. In contrast, mice lacking DGAT1 in adipocytes have normal TG storage on a chow diet but moderately decreased body fat accompanied by glucose intolerance when challenged with a high-fat diet. The latter changes were associated with the activation of ER stress pathways. We conclude that DGAT1 and DGAT2 can largely compensate for each other for TG storage but that DGAT1 uniquely has an important role in protecting the ER from the lipotoxic effects of high-fat diets.

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