Bioinspired nanostructured hydroxyapatite/collagen three-dimensional porous scaffolds for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (46) ◽  
pp. 36175-36184 ◽  
Author(s):  
Junjie Guan ◽  
Jun Yang ◽  
Junqi Dai ◽  
Yunhao Qin ◽  
Yang Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Needle Punching

A needle punching and bioinspired mineralization strategy has been developed to fabricate a collagen/hydroxyapatite porous scaffold for bone tissue engineering.

Fabrication of three-dimensional porous scaffold based on collagen fiber and bioglass for bone tissue engineering

2014 ◽  
Vol 103 (7) ◽  
pp. 1455-1464 ◽  
Author(s):  
Teng Long ◽  
Jun Yang ◽  
Shan-Shan Shi ◽  
Ya-Ping Guo ◽  
Qin-Fei Ke ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Fiber ◽  
Porous Scaffold ◽  
Three Dimensional

Hydroxyapatite/Biopolymers Composite Scaffolds for Bone Tissue Engineering

2011 ◽  
Vol 493-494 ◽  
pp. 826-831
Author(s):  
A.C.B.M. Fook ◽  
Thiago Bizerra Fideles ◽  
R.C. Barbosa ◽  
G.T.F.S. Furtado ◽  
G.Y.H. Sampaio ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
X Ray Diffraction ◽  
X Ray ◽  
Interconnected Pores

The application of a hybrid composite consisting of biopolymer and calcium phosphate, similar morphology and properties of natural bone, may be a way to solve the problem of the fragility of ceramics without reducing its mechanical properties, retaining the properties of biocompatibility and high bioactivity. This work aims at the preparation and characterization of three-dimensional scaffolds composite HA / biopolymers (chitosan and gelatin). The freeze-drying technique was employed in this study to obtain these frameworks and partial results showed the effectiveness of this method. This involved the study of structural, chemical and morphological frameworks, in order to direct the research suggested the application. The X Ray Diffraction (XRD) and infrared spectroscopy and Fourier transform (FTIR) results confirmed the formation of hydroxyapatite (HA) phase and the presence of characteristic bands of HA and biopolymers in all compositions. The microstructure of the scaffolds study conducted by Scanning Electron Microscopy (SEM) revealed the formation of longitudinally oriented microchannels with interconnected pores. In all compositions the porous scaffolds showed varying sizes and mostly larger than 100μm, and is therefore considered materials with potential for application in bone tissue engineering.

Fabrication of a chitosan/bioglass three-dimensional porous scaffold for bone tissue engineering applications

2014 ◽  
Vol 2 (38) ◽  
pp. 6611-6618 ◽  
Author(s):  
Jun Yang ◽  
Teng Long ◽  
Nan-Fei He ◽  
Ya-Ping Guo ◽  
Zhen-An Zhu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Bone Defects ◽  
Engineering Applications

A chitosan/bioglass three-dimensional porous scaffold with excellent biocompatibility and mechanical properties has been developed for the treatment of bone defects.

APPLICATIONS OF CALCIUM PHOSPHATE NANOPARTICLES IN POROUS HARD TISSUE ENGINEERING SCAFFOLDS

NANO ◽  
2012 ◽  
Vol 07 (04) ◽  
pp. 1230004 ◽  
Author(s):  
ZHE WANG ◽  
ZHURONG TANG ◽  
FANGZHU QING ◽  
YOULIANG HONG ◽  
XINGDONG ZHANG
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Important Approach ◽  
Calcium Phosphate Nanoparticles ◽  
Hard Tissue Engineering

To repair bone defects, an important approach is to fabricate tissue engineering scaffolds as substitutions to replace auto-/allologous bones. Currently, processing a biomaterial into three-dimensional porous scaffolds and incorporating the calcium phosphate (Ca–P) nanoparticles into scaffolds profile two main characteristics of bone tissue engineering scaffolds. Based on this fact, in this paper we describe the design principles of the Ca–P nanoparticle-based and porous bone tissue engineering scaffolds. Then we summarize a variety of the Ca–P nanoparticle-based scaffolds, including discussion of the integration of the Ca–P nanoparticles with ceramics and polymers, followed by introduction of safety of the Ca–P nanoparticles in scaffolds.

Structure and properties of nano-hydroxyapatite/poly(butylene succinate) porous scaffold for bone tissue engineering prepared by using ethanol as porogen

2018 ◽  
Vol 33 (6) ◽  
pp. 776-791 ◽  
Author(s):  
Gang Li ◽  
Shuhao Qin ◽  
Xiaonan Liu ◽  
Daohai Zhang ◽  
Min He
Keyword(s):  
Tissue Engineering ◽  
Pore Structure ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Degradation Products ◽  
Porous Scaffolds ◽  
Butylene Succinate ◽  
Uniform Dispersion ◽  
Polybutylene Succinate

Biodegradable polymers, because their degradation products are small molecules that do not cause immune system rejection, have been increasingly used by researchers to explore the preparation of scaffold with excellent mechanical properties, biocompatibility and biodegradability. In this study, nano-hydroxyapatite and polybutylene succinate were mixed by solution-blending to prepare a porous scaffold that could be used in the biomedical industry. Based on the viewpoint of bionics, porous scaffold with well pore structure and uniform dispersion of nano-hydroxyapatite particles was prepared using ethanol as a porogen. When ethanol was used as a porogen to prepare the porous scaffold, the effects of different mass ratios of nano-hydroxyapatite and polybutylene succinate on the porosity and pore structure of the porous scaffold were investigated under the same amount of ethanol. The mercury intrusion tests showed that the porosity of the 30 nano-hydroxyapatite/polybutylene succinate porous scaffold was 38.987%. The experiment results of in vitro mineralization and cell culture showed that the porous scaffolds have good osteogenic capacity and cell compatibility, including attachment and proliferation. All experiment results indicated that ethanol can be used as a porogen to prepare nano-hydroxyapatite/polybutylene succinate porous scaffold, and it has great potential as a scaffold for bone tissue engineering.

Conceptual design of three-dimensional scaffolds of powder-based materials for bone tissue engineering applications

2015 ◽  
Vol 21 (6) ◽  
pp. 716-724 ◽  
Author(s):  
Ramakrishna Vasireddi ◽  
Bikramjit Basu
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Bone Repair ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Content Type ◽  
Pore Sizes ◽  
Pore Size Distributions

Purpose – The purpose of this paper is to investigate the possibility to construct tissue-engineered bone repair scaffolds with pore size distributions using rapid prototyping techniques. Design/methodology/approach – The fabrication of porous scaffolds with complex porous architectures represents a major challenge in tissue engineering and the design aspects to mimic complex pore shape as well as spatial distribution of pore sizes of natural hard tissue remain unexplored. In this context, this work aims to evaluate the three-dimensional printing process to study its potential for scaffold fabrication as well as some innovative design of homogeneously porous or gradient porous scaffolds is described and such design has wider implication in the field of bone tissue engineering. Findings – The present work discusses biomedically relevant various design strategies with spatial/radial gradient in pore sizes as well as with different pore sizes and with different pore geometries. Originality/value – One of the important implications of the proposed novel design scheme would be the development of porous bioactive/biodegradable composites with gradient pore size, porosity, composition and with spatially distributed biochemical stimuli so that stem cells loaded into scaffolds would develop into complex tissues such as those at the bone–cartilage interface.

scholarly journals Characterization and In Vitro Assessment Of Three-Dimensional Extrusion Mg-Sr Codoped SiO2-Complexed Porous Micro-Hydroxyapatite Whisker Scaffolds For Bone Tissue Engineering

2021 ◽  
Author(s):  
Chengyong Li ◽  
Tingting Yan ◽  
Zhenkai Lou ◽  
Zhimin Jiang ◽  
Zhi Shi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Repair ◽  
Three Dimensional ◽  
Chemical Properties ◽  
Treatment Method ◽  
Porous Scaffolds ◽  
Active Elements

Abstract Background Orthopedics has made great progress with the development of medical treatment; however, large bone defects are still great challenges for orthopedic surgeons. A good bone substitute that can be obtained through bone tissue engineering may be an effective treatment method. Artificial hydroxyapatite is the main inorganic component of bones, but its applications are limited due to its fragility and lack of bone-active elements. Therefore, it is necessary to reduce its fragility and improve its biological activity. Methods In this study, we developed micro-hydroxyapatite whiskers (mHAws), which were doped with the essential trace active elements Mg2+ and Sr2+ through a low-temperature sintering technique, used silica complexes to improve the mechanical properties, and then manufactured the bionic porous scaffolds by extrusion molding and freeze-drying. Results Four types of scaffolds were obtained: mHAw-SiO2, Mg-doped mHAw-SiO2, Sr-doped mHAw-SiO2 and Mg-Sr-codoped mHAw-SiO2. These composite porous scaffolds have been suggested to have a sufficiently porous morphology with appropriate mechanical strength, are noncytotoxic, are able to support cell proliferation and spreading, and, more importantly, can promote the osteogenic differentiation of rBMSCs. Conclusion Therefore, these doped scaffolds not only have physical and chemical properties suitable for bone tissue engineering, but also have higher osteogenic bioactivity, and can be possibly serve as potential bone repair material.

Effect of Hydroxyapatite: Fibroin Ratio on Hydroxyapatite/Fibroin/Chitosan Porous Scaffold Characteristics

2016 ◽  
Vol 705 ◽  
pp. 315-319
Author(s):  
Wassanai Wattanutchariya ◽  
Anirut Chaijaruwanich ◽  
Tarin Sukhachiradet
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Porous Scaffolds ◽  
Fixed Amount ◽  
Replacement Technique ◽  
A New Technique

Autografting is a bone replacement technique used in orthopedic surgery. Bone tissue engineering is a new technique that offers promise, and could help alleviate this risk. Bioceramics, biopolymers or composite can be fabricated for artificial bone scaffold and used for bone regeneration. This study used three types of biomaterials – hydroxyapatite (HA), fibroin, and chitosan – to form porous scaffold. HA and fibroin were prepared from natural materials. HA was synthesized from mollusk shell by wet chemical precipitation method, while silk fibroin was extracted from silk worm’s cocoons. The HA and fibroin were mixed in a variety of ratios along with a fixed amount of chitosan before fabricating composite porous scaffolds by freeze-drying. The resulting scaffolds were evaluated for biodegradability, biocompatibility, porosity pore morphology and mechanical property. The fabricated scaffolds had an interconnected porous structure with a pore size of 200-400 μm and porosity in a range of 93-95%. The average degradation rate of the scaffold in lysozyme was between 7-17% at 7 days. A biocompatibility test showed that the scaffold was non-cytotoxic, making it a good candidate for future bone tissue engineering applications.

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