Designing bio-mimetic moxifloxacin loaded hydrogel wound dressing to improve antioxidant and pharmacology properties

The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.

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Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays

Journal of Applied Biomaterials & Functional Materials ◽

10.1177/2280800021989698 ◽

2021 ◽

Vol 19 ◽

pp. 228080002198969

Author(s):

Min-Xia Zhang ◽

Wan-Yi Zhao ◽

Qing-Qing Fang ◽

Xiao-Feng Wang ◽

Chun-Ye Chen ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Type I Collagen ◽

Inhibition Zone ◽

Negative Control ◽

Type I ◽

Nih3t3 Cells ◽

Post Operation

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

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Keratin Scaffolds Containing Casomorphin Stimulate Macrophage Infiltration and Accelerate Full-Thickness Cutaneous Wound Healing in Diabetic Mice

Molecules ◽

10.3390/molecules26092554 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2554

Author(s):

Marek Konop ◽

Anna K. Laskowska ◽

Mateusz Rybka ◽

Ewa Kłodzińska ◽

Dorota Sulejczak ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Healing Process ◽

Skin Wound ◽

Wound Healing Process ◽

Diabetic Mice ◽

Full Thickness ◽

Skin Wound Healing ◽

Impaired Wound Healing

Impaired wound healing is a major medical challenge, especially in diabetics. Over the centuries, the main goal of tissue engineering and regenerative medicine has been to invent biomaterials that accelerate the wound healing process. In this context, keratin-derived biomaterial is a promising candidate due to its biocompatibility and biodegradability. In this study, we evaluated an insoluble fraction of keratin containing casomorphin as a wound dressing in a full-thickness surgical skin wound model in mice (n = 20) with iatrogenically induced diabetes. Casomorphin, an opioid peptide with analgesic properties, was incorporated into keratin and shown to be slowly released from the dressing. An in vitro study showed that keratin-casomorphin dressing is biocompatible, non-toxic, and supports cell growth. In vivo experiments demonstrated that keratin-casomorphin dressing significantly (p < 0.05) accelerates the whole process of skin wound healing to the its final stage. Wounds covered with keratin-casomorphin dressing underwent reepithelization faster, ending up with a thicker epidermis than control wounds, as confirmed by histopathological and immunohistochemical examinations. This investigated dressing stimulated macrophages infiltration, which favors tissue remodeling and regeneration, unlike in the control wounds in which neutrophils predominated. Additionally, in dressed wounds, the number of microhemorrhages was significantly decreased (p < 0.05) as compared with control wounds. The dressing was naturally incorporated into regenerating tissue during the wound healing process. Applied keratin dressing favored reconstruction of more regular skin structure and assured better cosmetic outcome in terms of scar formation and appearance. Our results have shown that insoluble keratin wound dressing containing casomorphin supports skin wound healing in diabetic mice.

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Preparation of biocompatible wound dressings with dual release of antibiotic and platelet-rich plasma for enhancing infected wound healing

Journal of Biomaterials Applications ◽

10.1177/0885328221996013 ◽

2021 ◽

pp. 088532822199601

Author(s):

Linying Shi ◽

Fang Lin ◽

Mou Zhou ◽

Yanhui Li ◽

Wendan Li ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Platelet Rich Plasma ◽

Healing Process ◽

Wound Dressings ◽

Inflammatory Factors ◽

Gelatin Microspheres ◽

Infected Wounds ◽

Dual Release

The ever-growing threats of bacterial infection and chronic wound healing have provoked an urgent need for novel antibacterial wound dressings. In this study, we developed a wound dressing for the treatment of infected wounds, which can reduce the inflammatory period (through the use of gentamycin sulfate (GS)) and enhance the granulation stage (through the addition of platelet-rich plasma (PRP)). Herein, the sustained antimicrobial CMC/GMs@GS/PRP wound dressings were developed by using gelatin microspheres (GMs) loading GS and PRP, covalent bonding to carboxymethyl chitosan (CMC). The prepared dressings exhibited high water uptake capability, appropriate porosity, excellent mechanical properties, sustain release of PRP and GS. Meanwhile, the wound dressing showed good biocompatibility and excellent antibacterial ability against Gram-negative and Gram-positive bacteria. Moreover, in vivo experiments further demonstrated that the prepared dressings could accelerate the healing process of E. coli and S. aureus-infected full-thickness wounds i n vivo, reepithelialization, collagen deposition and angiogenesis. In addition, the treatment of CMC/GMs@GS/PRP wound dressing could reduce bacterial count, inhibit pro-inflammatory factors (TNF-α, IL-1β and IL-6), and enhance anti-inflammatory factors (TGF-β1). The findings of this study suggested that biocompatible wound dressings with dual release of GS and PRP have great potential in the treatment of chronic and infected wounds.

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Nanofiber Wound Dressing Materials—A Comparative Study of Wound Healing on a Porcine Model

Military Medicine ◽

10.1093/milmed/usab155 ◽

2021 ◽

Author(s):

Katerina Menclová ◽

Petr Svoboda ◽

Jan Hadač ◽

Štefan Juhás ◽

Jana Juhásová ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Blood Count ◽

Serum Amyloid A ◽

Porcine Model ◽

Serum Amyloid ◽

Wound Dressings ◽

Amyloid A ◽

Inflammatory Phase ◽

Dressing Materials

ABSTRACT Background Nanofiber wound dressings remain the domain of in vitro studies. The purpose of our study was to verify the benefits of chitosan (CTS) and polylactide (PLA)-based nanofiber wound dressings on a porcine model of a naturally contaminated standardized wound and compare them with the conventional dressings, i.e., gauze and Inadine. Material and Methods The study group included 32 pigs randomized into four homogeneous groups according to the wound dressing type. Standardized wounds were created on their backs, and wound dressings were regularly changed. We evaluated difficulty of handling individual dressing materials and macroscopic appearance of the wounds. Wound swabs were taken for bacteriological examination. Blood samples were obtained to determine blood count values and serum levels of acute phase proteins (serum amyloid A, C-reactive protein, and haptoglobin). The crucial point of the study was histological analysis. Microscopic evaluation was focused on the defect depth and tissue reactions, including formation of the fibrin exudate with neutrophil granulocytes, the layer of granulation and cellular connective tissue, and the reepithelialization. Statistical analysis was performed by using SPSS software. The analysis was based on the Kruskal–Wallis H test and Mann–Whitney U test followed by Bonferroni correction. Significance was set at P < .05. Results Macroscopic examination did not show any difference in wound healing among the groups. However, evaluation of histological findings demonstrated that PLA-based nanofiber dressing accelerated the proliferative (P = .025) and reepithelialization (P < .001) healing phases, while chitosan-based nanofiber dressing potentiated and accelerated the inflammatory phase (P = .006). No statistically significant changes were observed in the blood count or acute inflammatory phase proteins during the trial. Different dynamics were noted in serum amyloid A values in the group treated with PLA-based nanofiber dressing (P = .006). Conclusion Based on the microscopic examination, we have documented a positive effect of nanofiber wound dressings on acceleration of individual phases of the healing process. Nanofiber wound dressings have a potential to become in future part of the common wound care practice.

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Temperature-sensitive poly(N-isopropylacrylamide) (PNIPAAm) stabilized size controllable synthesis of silver nanoparticles and its improved antimicrobial activity for wound healing and nursing care after femoral fracture during surgery

Materials Express ◽

10.1166/mex.2021.1878 ◽

2021 ◽

Vol 11 (1) ◽

pp. 73-84

Author(s):

Xiaoyun Wang ◽

Jing Zhang

Keyword(s):

Wound Healing ◽

Silver Nanoparticles ◽

Femoral Fracture ◽

Bacterial Resistance ◽

Polymer Nanocomposite ◽

Temperature Sensitive ◽

Solution Stability ◽

Uniform Dispersion ◽

Significant Temperature ◽

Thermosensitive Copolymer

In this research, a thermo-sensitive copolymer PNIPAAm-co-MHq was used to successfully synthesize different nano sizes silver nanoparticles in the ranges between 1.5 to 4 nm with uniform dispersion. The polymeric assisted synthesized Ag nanoparticles (AgNPs@PM) exhibited reasonable solution stability and thermalresponsive behaviour. In specific, AgNPs@PM3 (1.59 nm) displayed improved bacterial resistance against clinically approved anti-biotic resistant bacterial pathigens with very low MIC value (4.05 μg/mL). Subsequently, the thermal responsive polymeric molecular structure on AgNPs synthesis has been established that significant temperature dependened anti-bacterial efficiency. It was also observed that the nonparticipants size, temperature responses and proportion of thermosensitive copolymer also influenced the antibacterial efficacy of AgNPs@PM. Resulting thermal sensitive polymer nanocomposite can be extremely beneficial for wound healing treatment after femoral fracture surgery.

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In-Vitro Wound Healing and Release Kinetics of β-Cyclodextrin Encapsulated Curcumin Loaded Carrageenan Hydrogel Film: An Efficient Wound Dressing Material

Proceedings of the International Conference on Nanomedicine (ICON-2019) - Springer Proceedings in Materials ◽

10.1007/978-3-030-25135-2_11 ◽

2019 ◽

pp. 107-119 ◽

Cited By ~ 1

Author(s):

Aswni Sundara Rajan ◽

Kerensa Miriam Sheen ◽

Balaji Sadhasivam ◽

Nachimuthu Saraswathy

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Release Kinetics ◽

Hydrogel Film ◽

Kinetics Of

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Photo-Crosslinked Polymeric Matrix with Antimicrobial Functions for Excisional Wound Healing in Mice

Nanomaterials ◽

10.3390/nano8100791 ◽

2018 ◽

Vol 8 (10) ◽

pp. 791 ◽

Cited By ~ 3

Author(s):

Ming-Hsiang Chang ◽

Yu-Ping Hsiao ◽

Chia-Yen Hsu ◽

Ping-Shan Lai

Keyword(s):

Wound Healing ◽

Wound Infection ◽

Polymer Solution ◽

Wound Dressing ◽

Glycol Diacrylate ◽

Excisional Wound ◽

Poly Ethylene ◽

Systemic Infections

Wound infection extends the duration of wound healing and also causes systemic infections such as sepsis, and, in severe cases, may lead to death. Early prevention of wound infection and its appropriate treatment are important. A photoreactive modified gelatin (GE-BTHE) was synthesized by gelatin and a conjugate formed from the 3,3′,4,4′-benzophenone tetracarboxylic dianhydride (BTDA) and the 2-hydroxyethyl methacrylate (HEMA). Herein, we investigated the photocurable polymer solution (GE-BTHE mixture) containing GE-BTHE, poly(ethylene glycol) diacrylate (PEGDA), chitosan, and methylene blue (MB), with antimicrobial functions and photodynamic antimicrobial chemotherapy for wound dressing. This photocurable polymer solution was found to have fast film-forming property attributed to the photochemical reaction between GE-BTHE and PEGDA, as well as the antibacterial activity in vitro attributed to the ingredients of chitosan and MB. Our in vivo results also demonstrated that untreated wounds after 3 days had the same scab level as the GE-BTHE mixture-treated wounds after 20 s of irradiation, which indicates that the irradiated GE-BTHE mixture can be quickly transferred into artificial scabs to protect wounds from an infection that can serve as a convenient excisional wound dressing with antibacterial efficacy. Therefore, it has the potential to treat nonhealing wounds, deep burns, diabetic ulcers and a variety of mucosal wounds.

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An innovative bi-layered wound dressing made of silk and gelatin for accelerated wound healing

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2012.06.046 ◽

2012 ◽

Vol 436 (1-2) ◽

pp. 141-153 ◽

Cited By ~ 90

Author(s):

Sorada Kanokpanont ◽

Siriporn Damrongsakkul ◽

Juthamas Ratanavaraporn ◽

Pornanong Aramwit

Keyword(s):

Wound Healing ◽

Wound Dressing

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