An acyclic enediyne with a furyl tethering group for efficient inhibition of tumor cell viability

2015 ◽  
Vol 3 (43) ◽  
pp. 8584-8588 ◽  
Author(s):  
Depeng Song ◽  
Yu Tian ◽  
Shuai Huang ◽  
Baojun Li ◽  
Yuan Yuan ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Viability ◽  
Design Strategy ◽  
Ph Sensitive ◽  
Antitumor Antibiotics ◽  
Normal Cells

An acyclic enediyne with a furyl tethering group and two pH-sensitive orthoester groups at the alkynyl termini was synthesized. The introduction of a furyl tethering group represents a new design strategy of “intelligent” antitumor antibiotics that can distinguish tumor and normal cells.

Using a hybrid radioenhancer to realize tumor cell-targeted treatment for osteosarcoma: an in vitro study

2020 ◽  
Vol 27 ◽  
Author(s):  
Fu-I Tung ◽  
Li-Chin Chen ◽  
Yu-Chi Wang ◽  
Ming-Hong Chen ◽  
Pei-Wei Shueng ◽  
...  
Keyword(s):  
Western Blot ◽  
Tumor Cell ◽  
Cell Viability ◽  
Tumor Cells ◽  
Clonogenic Assay ◽  
Normal Cells ◽  
Targeted Radiotherapy ◽  
In Vitro Investigation ◽  
Dose Radiation

: Osteosarcoma is insensitive to radiation. High-dose radiation is often used as a treatment, but causes side effects in patients. Hence, it is important to develop tumor cell-targeted radiotherapy that could improve radiotherapy efficiency on tumor cells and reduce the toxic effect on normal cells during radiation treatment. In this study, we developed an innovative method for treating osteosarcoma by using a novel radiation-enhancer (i.e., carboxymethyl-hexanoyl chitosan-coated selfassembled Au@Fe3O4 nanoparticles; CSAF NPs). CSAF NPs were employed together with 5-aminolevulinic acid (5-ALA) to achieve tumor cell-targeted radiotherapy. In this study, osteosarcoma cells (MG63) and normal cells (MC3T3-E1) were used for an in vitro investigation, in which a reactive oxygen species (ROS) assay, cell viability assay, clonogenic assay, and western blot were used to confirm the treatment efficiency. The ROS assay showed that the combination of CSAF NPs and 5-ALA enhanced radiation-induced ROS production in tumor cells (MG63); however, this was not observed in normal cells (MC3T3-E1). The cell viability ratio of normal cells to tumor cells after treatment with CSAF NPs and 5-ALA reached 2.79. Moreover, the clonogenic assay showed that the radiosensitivity of MG63 cells was increased by the combination use of CSAF NPs and 5-ALA. This was supported by performing a western blot that confirmed expression of cytochrome c (a marker of cell mitochondria damage) and caspase-3 (a marker of cell apoptosis). The results provide an essential basis for developing tumor-cell targeted radiotherapy by means of low-dose radiation.

BIOCOMPATIBILITY STUDY OF α-Fe2O3 NANOPARTICLES PREPARED BY HYDROTHERMAL METHOD

2019 ◽  
Vol 26 (09) ◽  
pp. 1950058
Author(s):  
SADEQ H. LAFTA ◽  
ALI ABDULRAHMAN TAHA ◽  
MUHAMMAD M. FARHAN ◽  
SHAIMA Y. ABDULFATTAH
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Hydrothermal Method ◽  
Ferric Oxide ◽  
Tumor Cell Line ◽  
Oxide Nanoparticles ◽  
Average Particle ◽  
Normal Cells ◽  
Prepared Nanoparticles ◽  
Biocompatibility Study

Nanoparticles of alpha ferric oxide ([Formula: see text]-Fe2O3) were prepared by the hydrothermal method. Structural properties of [Formula: see text]-Fe2O3 were determined by XRD, SEM and AFM measurements. The particles had a good matching with standard pattern. Average particle size was about 90[Formula: see text]nm and the distribution extended from about 20[Formula: see text]nm to 120[Formula: see text]nm. Biocompatibility study of ferric oxide nanoparticles against bacteria, parasites, tumor cell line and normal cells was determined. No antibacterial activity was observed for the concentration, of ferric oxide nanoparticles in distilled water, up to 1.5[Formula: see text]mg/ml vs. E. coli and S. aureus. Moreover, MTT assay was used to determine the cytotoxicity against parasites and cells. Intermediate cytotoxicity (53.30%) of 1.5[Formula: see text]mg/ml of prepared nanoparticles was noted against L. tropica, while weak cytotoxicity of 5.20% was observed against L. donovani at the same concentration of ferric oxide nanoparticles. On the other hand, the prepared nanoparticles revealed low cytotoxicity (47.28%) against SR tumor cell line, while no cytotoxicity was shown against lymphocytes, as a model of normal cells.

Antibodies against Mucin-Based Glycopeptides AffectTrypanosoma cruziCell Invasion and Tumor Cell Viability

ChemBioChem ◽  
2014 ◽  
Vol 15 (10) ◽  
pp. 1495-1507 ◽  
Author(s):  
Vanessa L. Campo ◽  
Thalita B. Riul ◽  
Ivone Carvalho ◽  
Marcelo-Dias Baruffi
Keyword(s):  
Tumor Cell ◽  
Cell Viability

Citrus flavonoid naringenin reduces mammary tumor cell viability, adipose mass, and adipose inflammation in obese ovariectomized mice

2017 ◽  
Vol 61 (9) ◽  
pp. 1600934 ◽  
Author(s):  
Jia-Yu Ke ◽  
Taylor Banh ◽  
Yung-Hsuan Hsiao ◽  
Rachel M. Cole ◽  
Shana R. Straka ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Viability ◽  
Mammary Tumor ◽  
Mammary Tumor Cell ◽  
Ovariectomized Mice ◽  
Adipose Inflammation ◽  
Adipose Mass

scholarly journals Assessing cell viability for physiological concentration of uric acid in normal cells

2019 ◽  
Vol 92 (0) ◽  
pp. 2-O-46
Author(s):  
Asuka Morita ◽  
Motoshi Ouchi ◽  
Keitaro Satoh ◽  
Ken-ichi Inoue ◽  
Keitaro Hayashi ◽  
...  
Keyword(s):  
Uric Acid ◽  
Cell Viability ◽  
Physiological Concentration ◽  
Normal Cells

scholarly journals Chk1 is Essential for Tumor Cell Viability Following Activation of the Replication Checkpoint

Cell Cycle ◽  
2004 ◽  
Vol 4 (1) ◽  
pp. 131-139 ◽  
Author(s):  
Song H. Cho ◽  
Christian D. Toouli ◽  
Gregory H. Fujii ◽  
Chad Crain ◽  
David Parry
Keyword(s):  
Tumor Cell ◽  
Cell Viability ◽  
Replication Checkpoint

scholarly journals Hepatocyte-tumor cell interaction in vitro. I. Conditions for rosette formation and inhibition by anti-H-2 antibody.

1980 ◽  
Vol 151 (4) ◽  
pp. 984-989 ◽  
Author(s):  
V Schirrmacher ◽  
R Cheingsong-Popov ◽  
H Arnheiter
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Metastatic Tumor ◽  
Cell Interaction ◽  
Rosette Formation ◽  
Normal Cells ◽  
Neuraminidase Treatment

Murine hepatocytes, isolated by an in situ collagenase-perfusion technique and cultured in Petri dishes, were shown to form rosettes with liver-metastasizing syngeneic tumor cells. Pretreatment of the tumor cells with neuraminidase generally increased the binding, whereas pretreatment of the liver cells with neuraminidase abolished the binding completely. The tumor-cell binding may be mediated by the previously described lectin-like receptor of hepatocytes that also was sensitive to neuraminidase treatment and that bound desialylated cells better than normal cells. Anti-H-2 sera could efficiently inhibit the rosette formation of metastatic tumor cells with the hepatocytes, which points to a possible role of H-2 molecules in this interaction of neoplastic and normal cells.

In Vitro Effect of Focused Ultrasound or Thermal Stress on HSP70 Expression and Cell Viability in Three Tumor Cell Lines

2007 ◽  
Vol 14 (7) ◽  
pp. 859-870 ◽  
Author(s):  
Walter Hundt ◽  
Caitlin E. O’Connell-Rodwell ◽  
Mark D. Bednarski ◽  
Silke Steinbach ◽  
Samira Guccione
Keyword(s):  
Thermal Stress ◽  
Tumor Cell ◽  
Cell Viability ◽  
Cell Lines ◽  
Focused Ultrasound ◽  
Hsp70 Expression ◽  
Tumor Cell Lines ◽  
Vitro Effect
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