scholarly journals Long-term protein packaging in cholinium-based ionic liquids: improved catalytic activity and enhanced stability of cytochrome c against multiple stresses

2017 ◽  
Vol 19 (20) ◽  
pp. 4900-4911 ◽  
Author(s):  
Meena Bisht ◽  
Dibyendu Mondal ◽  
Matheus M. Pereira ◽  
Mara G. Freire ◽  
P. Venkatesu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Catalytic Activity ◽  
Ionic Liquids ◽  
Cytochrome C ◽  
Multiple Stresses ◽  
Improved Stability ◽  
Cyt C ◽  
Enhanced Stability

Cholinium-based ionic liquids are shown to be promising packaging media of Cyt c with >50 fold enhancement in the enzyme activity and improved stability against multiple stresses.

Strategic planning of proteins in ionic liquids: future solvents for the enhanced stability of proteins against multiple stresses

2019 ◽  
Vol 21 (42) ◽  
pp. 23269-23282 ◽  
Author(s):  
Awanish Kumar ◽  
Kavya Bhakuni ◽  
Pannuru Venkatesu
Keyword(s):  
Ionic Liquids ◽  
Strategic Planning ◽  
Organic Solvents ◽  
Biomedical Applications ◽  
Protein Stabilization ◽  
Multiple Stresses ◽  
Toxic Organic Solvents ◽  
Enhanced Stability

Ionic liquids (ILs) represent as solvents or co-solvents for protein stabilization and refolding. Thus, ILs are replacement to toxic organic solvents in chemical, biotechnology and biomedical applications.

Engineering Cytochrome C with Quantum Dots and Ionic Liquids: A Win-Win Strategy for Protein Packaging against Multiple Stresses

2021 ◽  
Author(s):  
Sachin M. Shet ◽  
Sarath Kumar Thayallath ◽  
Meena Bisht ◽  
Matheus M. Pereira ◽  
João A. P. Coutinho ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Ionic Liquids ◽  
Cytochrome C ◽  
Multiple Stresses

scholarly journals Effect of confinement of horse heart cytochrome c and formate dehydrogenase from Candida boidinii on mesoporous carbons on their catalytic activity

2021 ◽  
Author(s):  
Naiara Hernández-Ibáñez ◽  
Vicente Montiel ◽  
Alicia Gomis-Berenguer ◽  
Conchi Ania ◽  
Jesús Iniesta
Keyword(s):  
Catalytic Activity ◽  
Cytochrome C ◽  
Formic Acid ◽  
Formate Dehydrogenase ◽  
Direct Electron Transfer ◽  
Candida Boidinii ◽  
Mesoporous Carbons ◽  
Horse Heart Cytochrome ◽  
Pore Sizes ◽  
Cyt C

AbstractThis study reports the immobilization of two biocatalysts (e.g., cytochrome c—Cyt c—and the non-metalloenzyme formate dehydrogenase from Candida boidinii–cbFDH) on a series of mesoporous carbons with controlled pore sizes. The catalytic activity of the nanoconfined proteins was correlated with the pore size distribution of the carbon materials used as supports. The electrochemical behaviour of nanoconfined Cyt c showed direct electron transfer electroactivity in pore sizes matching tightly the protein dimension. The pseudo-peroxidase activity towards H2O2 reduction was enhanced at pH 4.0, due to the protein conformational changes. For cbFDH, the reduction of CO2 towards formic acid was evaluated for the nanoconfined protein, in the presence of nicotinamide adenine dinucleotide (NADH). The carbons displayed different cbFDH uptake capacity, governed by the dimensions of the main mesopore cavities and their accessibility through narrow pore necks. The catalytic activity of nanoconfined cbFDH was largely improved, compared to its performance in free solution. Regardless of the carbon support used, the production of formic acid was higher upon immobilization with lower nominal cbFDH:NADH ratios.

scholarly journals Biomimetic Mineralization of Cytochrome c Improves the Catalytic Efficiency and Confers a Functional Multi-Enzyme Composite

Catalysts ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 648 ◽  
Author(s):  
Xiao-Qing Gong ◽  
Chuan-Wan Wei ◽  
Jia-Kun Xu ◽  
Xiao-Juan Wang ◽  
Shu-Qin Gao ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Enzymatic Activity ◽  
Catalytic Efficiency ◽  
Biomimetic Mineralization ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Metal Organic ◽  
Cyt C ◽  
Increased Activity

The encapsulated enzyme system by metal-organic frameworks (MOFs) exhibits great potential in biofuel cells, pharmaceuticals, and biocatalysis. However, the catalytic efficiency and the enzymatic activity are severely hampered due to enzyme leaching and deficiency of storage stability. In this study, we immobilized cytochrome c (Cyt c) into dimethylimidazole-copper (Cu(Im)2) by biomimetic mineralization, and constructed a bioinorganic hybrid material, termed Cyt c@Cu(Im)2. Encapsulated Cyt c in Cu(Im)2 with a nanosheet structure exhibited significantly improved catalytic efficiency, enzymatic activity and kinetic performance. The catalytic efficiency (kcat/Km) for Cyt c@Cu(Im)2 was ~20-fold higher compared to that of free Cyt c. Moreover, the increased activity was not affected by long-term storage. Based on this system, we further constructed a multi-enzyme composite with glucose-oxidase (GOx), termed GOx-Cyt c@Cu(Im)2, which exhibited greatly improved enzymatic activity, stability, and excellent selectivity for the detection of low concentrations of glucose. This strategy may provide new insights into the design of enzymes with high activity and stability, as well as the construction of multi-enzyme systems.

scholarly journals Treatment of the Fluoroquinolone-Associated Disability: The Pathobiochemical Implications

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Krzysztof Michalak ◽  
Aleksandra Sobolewska-Włodarczyk ◽  
Marcin Włodarczyk ◽  
Justyna Sobolewska ◽  
Piotr Woźniak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Enzyme Activity ◽  
Antibiotic Therapy ◽  
Effective Treatment ◽  
Social Problem ◽  
Fenton Reaction ◽  
Current Paper ◽  
Bivalent Cations

Long-term fluoroquinolone-associated disability (FQAD) after fluoroquinolone (FQ) antibiotic therapy appears in recent years as a significant medical and social problem, because patients suffer for many years after prescribed antimicrobial FQ treatment from tiredness, concentration problems, neuropathies, tendinopathies, and other symptoms. The knowledge about the molecular activity of FQs in the cells remains unclear in many details. The effective treatment of this chronic state remains difficult and not effective. The current paper reviews the pathobiochemical properties of FQs, hints the directions for further research, and reviews the research concerning the proposed treatment of patients. Based on the analysis of literature, the main directions of possible effective treatment of FQAD are proposed: (a) reduction of the oxidative stress, (b) restoring reduced mitochondrion potential ΔΨm, (c) supplementation of uni- and bivalent cations that are chelated by FQs and probably ineffectively transported to the cell (caution must be paid to Fe and Cu because they may generate Fenton reaction), (d) stimulating the mitochondrial proliferation, (e) removing FQs permanently accumulated in the cells (if this phenomenon takes place), and (f) regulating the disturbed gene expression and enzyme activity.

Enhanced stability of conductive polyacetylene in ladder-like surface-grafted brushes

2016 ◽  
Vol 7 (36) ◽  
pp. 5664-5670 ◽  
Author(s):  
Michał Szuwarzyński ◽  
Karol Wolski ◽  
Szczepan Zapotoczny
Keyword(s):  
Term Stability ◽  
Long Term Stability ◽  
Enhanced Stability

Formation and characterization of polyacetylene-based brushes that exhibit exceptional long term stability in air is presented here.

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