AIE active piperazine appended naphthalimide-BODIPYs: photophysical properties and applications in live cell lysosomal tracking

The Analyst ◽  
2019 ◽  
Vol 144 (1) ◽  
pp. 331-341 ◽  
Author(s):  
Bhupendra Kumar Dwivedi ◽  
Roop Shikha Singh ◽  
Afsar Ali ◽  
Vinay Sharma ◽  
Shaikh M. Mobin ◽  
...  
Keyword(s):  
High Selectivity ◽  
Photophysical Properties ◽  
Living Cells ◽  
Live Cell ◽  
Aggregation Induced Emission ◽  
Lysosomal Ph

Piperazine appended naphthalimide-BODIPYs (NPB1–NPB4) exhibiting solvatochromism, aggregation-induced emission, and high selectivity towards lysosomal pH in living cells have been described.

scholarly journals A Novel Coumarin-Based Fluorescent Probe With Aggregation Induced Emission for Detecting Cn− and Its Applications in Bioimaging

2021 ◽  
Author(s):  
Sha Deng ◽  
Qiao Lei ◽  
Junzhe Cai ◽  
Yuliang Jiang ◽  
Jian Shen
Keyword(s):  
Optical Properties ◽  
Excited State ◽  
High Selectivity ◽  
High Sensitivity ◽  
Intramolecular Proton Transfer ◽  
Living Cells ◽  
Aggregation Induced Emission ◽  
Channel Response ◽  
Dual Channel ◽  
Low Toxicity

Abstract Although cyanogen ion (CN-) plays important role in industry which also bring acute environmental pollution. More serious, trace CN- enters the human body can cause serious consequences and even death. Therefore, it is of great significance to detect trace CN- with high sensitivity. Herein, a novel aggregation-induced emission (AIE) probe C-BH was synthesized based on coumarin matrix. Probe C-BH showed high selectivity and sensitivity toward CN- by dual channel response due to the excited state intramolecular proton transfer (ESIPT). The low detection limit was calculated to be 0.05 µM. Moreover, probe C-BH was successfully used for imaging CN- in living cells and zebrafish due to its low toxicity and excellent optical properties.

A rhodamine B-based lysosomal pH probe

2015 ◽  
Vol 3 (5) ◽  
pp. 919-925 ◽  
Author(s):  
Shi-Li Shen ◽  
Xin-Peng Chen ◽  
Xiao-Fan Zhang ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Response Time ◽  
Rhodamine B ◽  
High Selectivity ◽  
High Sensitivity ◽  
Living Cells ◽  
Acidic Ph ◽  
Ph Change ◽  
Short Response Time ◽  
Lysosomal Ph ◽  
Ph Probe

A novel rhodamine B-based lysosomal pH probe RML was developed. RML responded to acidic pH with short response time, high selectivity and high sensitivity and could detect lysosomal pH change in living cells.

Fluorescent sensor based on triphenylamine for Zn2+ with high selectivity and imaging in living cells

2021 ◽  
Vol 251 ◽  
pp. 119480
Author(s):  
Dongyuan Wang ◽  
Qiang Yin ◽  
Ming Zheng ◽  
Yongbo Xie ◽  
Wen He ◽  
...  
Keyword(s):  
High Selectivity ◽  
Fluorescent Sensor ◽  
Living Cells

scholarly journals Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Linda S. Forero-Quintero ◽  
William Raymond ◽  
Tetsuya Handa ◽  
Matthew N. Saxton ◽  
Tatsuya Morisaki ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Single Molecule ◽  
Computational Models ◽  
Spatial Organization ◽  
Fluorescent Antibody ◽  
Single Gene ◽  
Single Copy ◽  
Living Cells ◽  
Live Cell

AbstractThe carboxyl-terminal domain of RNA polymerase II (RNAP2) is phosphorylated during transcription in eukaryotic cells. While residue-specific phosphorylation has been mapped with exquisite spatial resolution along the 1D genome in a population of fixed cells using immunoprecipitation-based assays, the timing, kinetics, and spatial organization of phosphorylation along a single-copy gene have not yet been measured in living cells. Here, we achieve this by combining multi-color, single-molecule microscopy with fluorescent antibody-based probes that specifically bind to different phosphorylated forms of endogenous RNAP2 in living cells. Applying this methodology to a single-copy HIV-1 reporter gene provides live-cell evidence for heterogeneity in the distribution of RNAP2 along the length of the gene as well as Serine 5 phosphorylated RNAP2 clusters that remain separated in both space and time from nascent mRNA synthesis. Computational models determine that 5 to 40 RNAP2 cluster around the promoter during a typical transcriptional burst, with most phosphorylated at Serine 5 within 6 seconds of arrival and roughly half escaping the promoter in ~1.5 minutes. Taken together, our data provide live-cell support for the notion of efficient transcription clusters that transiently form around promoters and contain high concentrations of RNAP2 phosphorylated at Serine 5.

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

A ratiometric fluorescence sensor for HOCl based on a FRET platform and application in living cells

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17059-17063 ◽  
Author(s):  
Yan-Ru Zhang ◽  
Zhi-Min Zhao ◽  
Le Su ◽  
Jun-Ying Miao ◽  
Bao-Xiang Zhao
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Fast Response ◽  
Living Cells ◽  
Fluorescence Sensor ◽  
Ratiometric Fluorescence ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe CRSH based on a FRET platform for detecting HOCl. CRSH showed high selectivity, excellent sensitivity and a fast response toward HOCl.

Use of rhodamine-allyl Schiff base in chemodosimetric processes for total palladium estimation and application in live cell imaging

2018 ◽  
Vol 42 (21) ◽  
pp. 17351-17358 ◽  
Author(s):  
Anup Kumar Bhanja ◽  
Snehasis Mishra ◽  
Ketaki Kar ◽  
Kaushik Naskar ◽  
Suvendu Maity ◽  
...  
Keyword(s):  
Schiff Base ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

An allyl-rhodamine Schiff base shows excellent palladium sensitivity (LOD, 95 nM) irrespective of Pd(0,ii,iv) and practical applicability is judged in living cells of RAW 264.7 (macrophage) cells.

A new ratiometric fluorescent probe for detecting endogenous HClO in living cells

2018 ◽  
Vol 42 (4) ◽  
pp. 2989-2993 ◽  
Author(s):  
Li-Jie Zhang ◽  
Jun-Ya Ning ◽  
Jun-Ying Miao ◽  
Jin-Ting Liu ◽  
Bao-Xiang Zhao
Keyword(s):  
Fluorescent Probe ◽  
High Selectivity ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

A new benzimidazole-hemicyanine-based ratiometric fluorescent probe (ZBM-H) was developed, which showed high selectivity and sensitivity for detecting HClO.

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