A novel bifunctional chemosensor for bioimaging in living cells with highly sensitive colorimetric and fluorescent detection of CN- and Al3+

A novel colorimetric/fluorescent chemosensor (1o) was designed and synthesized for the detection of CN- and Al3+. 1o exhibited a selective dual channel response to CN-, while it showed fluorescence changes...

DFT-Based Channel Estimation with Channel Response Mirroring for MIMO OFDM Systems

In this paper, DFT-Based channel estimation with channel response mirroring is proposed and analyzed. In General, pilot symbols for channel estimation in MIMO(Multi-Input Multi-Output) OFDM(Orthogonal Frequency-Division Multiplexing) Systems have a diamond shape in the time-frequency plane. An interpolation technique to estimate the channel response of sub-carriers between reference symbols is needed. Various interpolation techniques such as linear interpolation, low-pass filtering interpolation, cubic interpolation and DFT interpolation are employed to estimate the non-pilot sub-carriers. In this paper, we investigate the conventional DFT-based channel estimation for noise reduction and channel response interpolation. The conventional method has performance degradation by distortion called “edge effect” or “border effect”. In order to mitigate the distortion, we propose an improved DFT-based channel estimation with channel response mirroring. This technique can efficiently mitigate the distortion caused by the DFT of channel response discontinuity. Simulation results show that the proposed method has better performance than the conventional DFT-based channel estimation in terms of MSE.

Subtype specific responses in hKv7.4 and hKv7.5 channels to polyunsaturated fatty acids

The KV7.4 and KV7.5 subtypes of voltage-gated potassium channels are expressed in several tissues where they play a role in physiological processes such as sound amplification in the cochlea and adjusting vascular smooth muscle tone. Therefore, the mechanisms that regulate KV7.4 and KV7.5 channel function are of interest. Here, we study the effect of polyunsaturated fatty acids (PUFAs) on human KV7.4 and KV7.5 channels expressed in Xenopus oocytes. We report that KV7.5 is activated by PUFAs, which shift the V50 of the conductance versus voltage (G(V)) curve towards more negative voltages. This response depends on the charge of the head group as an uncharged PUFA analogue has no effect and a positively charged PUFA analogue induces positive V50 shifts. In contrast, we find that the KV7.4 channel is inhibited by PUFAs, which shift V50 towards more positive voltages. No effect on V50 of KV7.4 is observed by an uncharged or a positively charged PUFA analogue. Oocytes co-expressing KV7.4 and KV7.5 display an intermediate response to PUFAs. Altogether, the KV7.5 channel's response to PUFAs is like that previously observed in KV7.1-7.3 channels, whereas the KV7.4 channel response is opposite, revealing subtype specific responses to PUFAs.

An outer-pore gate modulates the pharmacology of the TMEM16A channel

TMEM16A Ca2+-activated chloride channels are involved in multiple cellular functions and are proposed targets for diseases such as hypertension, stroke, and cystic fibrosis. This therapeutic endeavor, however, suffers from paucity of selective and potent modulators. Here, exploiting a synthetic small molecule with a biphasic effect on the TMEM16A channel, anthracene-9-carboxylic acid (A9C), we shed light on sites of the channel amenable for pharmacological intervention. Mutant channels with the intracellular gate constitutively open were generated. These channels were entirely insensitive to extracellular A9C when intracellular Ca2+ was omitted. However, when physiological Ca2+ levels were reestablished, the mutants regained sensitivity to A9C. Thus, intracellular Ca2+ is mandatory for the channel response to an extracellular modulator. The underlying mechanism is a conformational change in the outer pore that enables A9C to enter the pore to reach its binding site. The explanation of this structural rearrangement highlights a critical site for pharmacological intervention and reveals an aspect of Ca2+ gating in the TMEM16A channel.

Signal Classification Algorithms over Time Selective Channels

In this work, we propose a general framework to design a signal classification algorithm over time selective channels for wireless communications applications. We derive an upper bound on the maximum number of observation samples over which the channel response is an essential invariant. The proposed framework relies on dividing the received signal into blocks, and each of them has a length less than the mentioned bound. Then, these blocks are fed into a number of classifiers in a parallel fashion. A final decision is made through a well-designed combiner and detector. As a case study, we employ the proposed framework on a space-time block-code classification problem by developing two combiners and detectors. Monte Carlo simulations show that the proposed framework is capable of achieving excellent classification performance over time selective channels compared to the conventional algorithms.

A Novel Coumarin-Based Fluorescent Probe With Aggregation Induced Emission for Detecting Cn− and Its Applications in Bioimaging

Although cyanogen ion (CN-) plays important role in industry which also bring acute environmental pollution. More serious, trace CN- enters the human body can cause serious consequences and even death. Therefore, it is of great significance to detect trace CN- with high sensitivity. Herein, a novel aggregation-induced emission (AIE) probe C-BH was synthesized based on coumarin matrix. Probe C-BH showed high selectivity and sensitivity toward CN- by dual channel response due to the excited state intramolecular proton transfer (ESIPT). The low detection limit was calculated to be 0.05 µM. Moreover, probe C-BH was successfully used for imaging CN- in living cells and zebrafish due to its low toxicity and excellent optical properties.