Ligand-mediated reversal of the oxidation state dependent ROS scavenging and enzyme mimicking activity of ceria nanoparticles

The present paper describes a versatile route to modify and stabilize ceria nanoparticles with a radical initiator, 4,4′-azobis(4-cyanovaleric acid) (ACVA), allowing a strong interface to be formed via grafting of polymers from the surface.

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Different approaches to synthesise cerium oxide nanoparticles and their corresponding physical characteristics, ROS scavenging and anti-inflammatory capabilities

Journal of Materials Chemistry B ◽

10.1039/d1tb01091c ◽

2021 ◽

Author(s):

Yuao Wu ◽

Hang Thu Ta

Keyword(s):

Cerium Oxide ◽

Physical Characteristics ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Biological Applications ◽

Ros Scavenging ◽

Trivalent Cerium ◽

Anti Inflammatory ◽

Tetravalent Cerium

The biological applications of cerium oxide nanoparticles (nanoceria) have received extensive attention in recent decades. The coexistence of trivalent cerium and tetravalent cerium on the surface of nanoceria allows the...

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Poly(acrylic acid)-mediated synthesis of cerium oxide nanoparticles with variable oxidation state and their effect on regulating intracellular ROS level

Journal of Materials Chemistry B ◽

10.1039/d1tb00706h ◽

2021 ◽

Author(s):

Xiaohui Ju ◽

Marie Hubalek Kalbacova ◽

Břetislav Šmíd ◽

Viktor Johánek ◽

Martin Janata ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Acrylic Acid ◽

Cerium Oxide ◽

Oxidation State ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Oxygen Species ◽

Redox Enzyme ◽

Enzyme Mimetic ◽

Intracellular Ros

Cerium oxide nanoparticles (CeNPs) possess multiple redox enzyme mimetic activities to scavenge reactive oxygen species (ROS) as potential biomedicine. These enzymatic activities of CeNPs are closely related to their surface...

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Contraction, cation oxidation state and size effects in cerium oxide nanoparticles

Nanotechnology ◽

10.1088/1361-6528/aa926f ◽

2017 ◽

Vol 28 (49) ◽

pp. 495702 ◽

Cited By ~ 3

Author(s):

Jacopo Stefano Pelli Cresi ◽

Maria Chiara Spadaro ◽

Sergio D’Addato ◽

Sergio Valeri ◽

Lucia Amidani ◽

...

Keyword(s):

Cerium Oxide ◽

Oxidation State ◽

Size Effects ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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Cerium Oxide Nanoparticles: A Brief Review of Their Synthesis Methods and Biomedical Applications

Antioxidants ◽

10.3390/antiox7080097 ◽

2018 ◽

Vol 7 (8) ◽

pp. 97 ◽

Cited By ~ 94

Author(s):

Atul Dhall ◽

William Self

Keyword(s):

Cerium Oxide ◽

Biomedical Applications ◽

Antioxidant Properties ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Synthesis Methods ◽

Ros Scavenging ◽

Corona Formation

Cerium oxide nanoparticles (CeNPs) exhibit antioxidant properties both in vitro and in vivo. This is due to the self-regeneration of their surface, which is based on redox-cycling between 3+ and 4+ states for cerium, in response to their immediate environment. Additionally, oxygen vacancies in the lattice structure allow for alternating between CeO2 and CeO2−x during redox reactions. Research to identify and characterize the biomedical applications of CeNPs has been heavily focused on investigating their use in treating diseases that are characterized by higher levels of reactive oxygen species (ROS). Although the bio-mimetic activities of CeNPs have been extensively studied in vitro, in vivo interactions and associated protein corona formation are not well understood. This review describes: (1) the methods of synthesis for CeNPs, including the recent green synthesis methods that offer enhanced biocompatibility and a need for establishing a reference CeNP material for consistency across studies; (2) their enzyme-mimetic activities, with a focus on their antioxidant activities; and, (3) recent experimental evidence that demonstrates their ROS scavenging abilities and their potential use in personalized medicine.

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Aminopolycarboxylic acids as a versatile tool to stabilize ceria nanoparticles – a fundamental model experimentally demonstrated

RSC Advances ◽

10.1039/c3ra45875j ◽

2014 ◽

Vol 4 (18) ◽

pp. 9048-9055 ◽

Cited By ~ 11

Author(s):

Eric Johansson Salazar-Sandoval ◽

Mats K. G. Johansson ◽

Anwar Ahniyaz

Keyword(s):

Cerium Oxide ◽

Nitrilotriacetic Acid ◽

Colloidal Synthesis ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Ceria Nanoparticles ◽

Water Dispersion ◽

Fundamental Model ◽

Aminopolycarboxylic Acids ◽

Versatile Tool

An extremely stable water dispersion of cerium oxide nanoparticles was prepared by colloidal synthesis, using nitrilotriacetic acid (NTA) as a stabilizer.

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Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes

International Journal of Molecular Sciences ◽

10.3390/ijms20235959 ◽

2019 ◽

Vol 20 (23) ◽

pp. 5959 ◽

Cited By ~ 2

Author(s):

Silvia Carvajal ◽

Meritxell Perramón ◽

Gregori Casals ◽

Denise Oró ◽

Jordi Ribera ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Viability ◽

Cerium Oxide ◽

Antioxidant Properties ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Oxidant Injury ◽

Ros Scavenging ◽

Alcoholic Fatty Liver ◽

The Impact

Cerium oxide nanoparticles (CeO2NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO2NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO2NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO2NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO2NPs did not modify HepG2 cell viability in basal conditions but reduced H2O2- and lipopolysaccharide (LPS)-induced cell death and prevented H2O2-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO2NPs reverted the H2O2-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H2O2 effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO2NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways.

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Polyacrylic Acid Modified Cerium Oxide Nanoparticles: Synthesis and Characterization as a Peroxidase Mimic for Non-Enzymatic H2O2 Sensor

Current Nanoscience ◽

10.2174/1573413715666191204124329 ◽

2020 ◽

Vol 16 (5) ◽

pp. 816-828

Author(s):

Gurdeep Rattu ◽

Nishtha Khansili ◽

Prayaga M. Krishna

Keyword(s):

Hydrogen Peroxide ◽

Cerium Oxide ◽

Polyacrylic Acid ◽

Spectroscopic Analysis ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Peroxidase Mimic ◽

Ft Ir ◽

H2o2 Sensing ◽

Fluorescence Spectrometer

Background: Cerium oxide nanoparticles (nanoceria) are efficient free-radical scavengers due to their dual valence state and thus exhibit optical and catalytic properties. Therefore, the main purpose of this work was to understand the peroxidase mimic activity of polymer-stabilized nanoceria for enzyme-less H2O2 sensing by fluorescence spectrometer. Objective: This research revealed the development of fluorescence hydrogen peroxide nanosensor based on the peroxidase-like activity of polyacrylic acid stabilized nanoceria (PAA-CeO2 Nps). Methods: PAA-CeO2 Nps were synthesized by simple cross-linking reaction at a low temperature and characterized by XRD, SEM, Zeta potential, TGA, FT-IR and UV-VIS spectroscopic analysis. H2O2 sensing was performed by a fluorescence spectrometer. Results:: The synthesized polymer nanocomposite was characterized by XRD, SEM, TGA, FT-IR and UV-VIS spectroscopic analysis. The XRD diffraction patterns confirmed the polycrystalline nature and SEM micrograph showed nanoparticles having hexagonal symmetry and crystallite size of 32 nm. The broad peak of Ce–O bond appeared at 508 cm-1. UV-VIS measurements revealed a welldefined absorbance peak around 315 nm and an optical band-gap of 3.17 eV. As synthesized PAACeO2 Nps effectively catalysed the decomposition of hydrogen peroxide (H2O2) into hydroxyl radicals. Then terephthalic acid was oxidized by hydroxyl radical to form a highly fluorescent product. Under optimized conditions, the linear range for determination of hydrogen peroxide was 0.01 - 0.2 mM with a limit of detection (LOD) of 1.2 μM. Conclusion: The proposed method is ideally suited for the sensing of H2O2 at a low cost and this detection system enabled the sensing of analytes (sugars), which can enzymatically generate hydrogen peroxide.

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