Propidium monoazide pretreatment on a 3D-printed microfluidic device for efficient PCR determination of ‘liveversusdead’ microbial cells

ABSTRACT This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.

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Evaluation of the Ability to Accurately Produce Angular Details by 3D Printing of Plastic Parts

Machines ◽

10.3390/machines9080150 ◽

2021 ◽

Vol 9 (8) ◽

pp. 150

Author(s):

Andrei Marius Mihalache ◽

Gheorghe Nagîț ◽

Laurențiu Slătineanu ◽

Adelina Hrițuc ◽

Angelos Markopoulos ◽

...

Keyword(s):

3D Printing ◽

Point Of View ◽

Vertex Angle ◽

Function Type ◽

Constant Height ◽

3D Printers ◽

3D Printed ◽

Mathematical Processing ◽

Plastic Parts

3D printing is a process that has become widely used in recent years, allowing the production of parts with relatively complicated shapes from metallic and non-metallic materials. In some cases, it is challenging to evaluate the ability of 3D printers to make fine details of parts. For such an assessment, the printing of samples showing intersections of surfaces with low angle values was considered. An experimental plan was designed and materialized to highlight the influence of different factors, such as the thickness of the deposited material layer, the printing speed, the cooling and filling conditions of the 3D-printed part, and the thickness of the sample. Samples using areas in the form of isosceles triangles with constant height or bases with the same length, respectively, were used. The mathematical processing of the experimental results allowed the determination of empirical mathematical models of the power-function type. It allowed the detection of both the direction of actions and the intensity of the influence exerted by the input factors. It is concluded that the strongest influence on the printer’s ability to produce fine detail, from the point of view addressed in the paper, is exerted by the vertex angle, whose reduction leads to a decrease in printing accuracy.

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Colorimetric Determination of Sulphur Dioxide and Sulphites in Various Environmental Samples

Journal of the Chinese Chemical Society ◽

10.1002/jccs.200300038 ◽

2003 ◽

Vol 50 (2) ◽

pp. 257-260 ◽

Cited By ~ 2

Author(s):

Sukumar Chatterjee ◽

Ajai K. Pillai ◽

V. K. Gupta

Keyword(s):

Sulphur Dioxide ◽

Environmental Samples ◽

Colorimetric Determination

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Field analysis Cr(VI) in water samples by a smartphone-based ultralong absorption path reflection colorimetric device

New Journal of Chemistry ◽

10.1039/d0nj05948j ◽

2021 ◽

Author(s):

Xiaolan Chen ◽

Cheng Ma ◽

Qi Kang ◽

Yu-Qin Chen ◽

Dazhong Shen

Keyword(s):

Hexavalent Chromium ◽

Water Samples ◽

Environmental Samples ◽

Field Analysis ◽

Absorption Path

Hexavalent chromium is highly toxic and regarded as one of the main pollutants in environmental samples. Sophisticated laboratory instruments are commercially available for in-field determination of chromium. It is highly...

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The universal dual-mode aptasensor for simultaneous determination of different bacteria based on naked eyes and microfluidic-chip together with magnetic DNA encoded probes

Talanta ◽

10.1016/j.talanta.2020.122062 ◽

2021 ◽

Vol 225 ◽

pp. 122062

Author(s):

Jiale Yu ◽

Huihui Wu ◽

Liyong He ◽

Lei Tan ◽

Zhijian Jia ◽

...

Keyword(s):

Simultaneous Determination ◽

Microfluidic Chip ◽

Dual Mode

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Interfacial Tension Sensor for Low Dosage Surfactant Detection

Colloids and Interfaces ◽

10.3390/colloids5010009 ◽

2021 ◽

Vol 5 (1) ◽

pp. 9

Author(s):

Piotr Pawliszak ◽

Bronwyn H. Bradshaw-Hajek ◽

Christopher Greet ◽

William Skinner ◽

David A. Beattie ◽

...

Keyword(s):

Interfacial Tension ◽

Microfluidic Device ◽

Profile Analysis ◽

Simple Method ◽

Bubble Generation ◽

Flotation Process ◽

Line Measurement ◽

Tension Sensor ◽

Low Dosage

Currently there are no available methods for in-line measurement of gas-liquid interfacial tension during the flotation process. Microfluidic devices have the potential to be deployed in such settings to allow for a rapid in-line determination of the interfacial tension, and hence provide information on frother concentration. This paper presents the development of a simple method for interfacial tension determination based on a microfluidic device with a flow-focusing geometry. The bubble generation frequency in such a microfluidic device is correlated with the concentration of two flotation frothers (characterized by very different adsorption kinetic behavior). The results are compared with the equilibrium interfacial tension values determined using classical profile analysis tensiometry.

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