Optimization of the sample preparation method for adherent cell metabolomics based on ultra-performance liquid chromatography coupled to mass spectrometry

2019 ◽  
Vol 11 (29) ◽  
pp. 3678-3686 ◽  
Author(s):  
Kun Zhang ◽  
Xiaohan Zhang ◽  
Yunfan Bai ◽  
Lijun Yang ◽  
Tong Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Preparation Method ◽  
Ultra Performance Liquid Chromatography ◽  
Adherent Cell ◽  
Sample Preparation Method ◽  
Cell Metabolomics ◽  
Cells Cultured ◽  
Satisfactory Quality

Sample preparation plays a crucial role in generating data with satisfactory quality and stability for metabolomics studies, especially those conducted on cells cultured in vitro.

scholarly journals Determination of trans fatty acids in foodstuffs by gas chromatography-mass spectrometry after simultaneous microwave-assisted extraction-esterification

2010 ◽  
Vol 75 (6) ◽  
pp. 803-812 ◽  
Author(s):  
Snezana Kravic ◽  
Zvonimir Suturovic ◽  
Jaroslava Svarc-Gajic ◽  
Zorica Stojanovic ◽  
Mira Pucarevic
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Preparation Method ◽  
Reference Method ◽  
Gas Chromatography Mass Spectrometry ◽  
Microwave Assisted Extraction ◽  
Sample Preparation Method ◽  
Microwave Assisted ◽  
Assisted Extraction

A sample preparation method based on the simultaneous microwave-assisted extraction-esterification (SMAEE) was developed for the determination of the fatty acid composition of foodstuffs by gas chromatography-mass spectrometry. The proposed sample preparation method was validated by comparison with the reference Soxhlet extraction method followed by derivatisation by ester formation and the same determination step. The fatty acid compositions and the extraction efficiencies obtained using the proposed SMAEE method and the reference method were statistically similar. The results showed that compared to the conventional method, the SMAEE method offered the advantages of short sample preparation time, low consumption of expensive organic solvents and lower energy consumption. This good agreement between results provided by both the SMAEE and the reference method demonstrates the usefulness of the former as a routine method for the treatment of food samples prior to trans fatty analysis.

scholarly journals Profiling embryonic stem cell differentiation by MALDI TOF mass spectrometry: development of a reproducible and robust sample preparation workflow

The Analyst ◽  
2019 ◽  
Vol 144 (21) ◽  
pp. 6371-6381 ◽  
Author(s):  
Rachel E. Heap ◽  
Anna Segarra-Fas ◽  
Alasdair P. Blain ◽  
Greg M. Findlay ◽  
Matthias Trost
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mammalian Cells ◽  
Preparation Method ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Sample Preparation Method ◽  
Maldi Tof ◽  
Tof Ms

A fast and robust sample preparation method for MALDI TOF MS analysis of whole mammalian cells and phenotypic profiling of mESCs.

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